This dataset is a document describing the Pelagic Tunicates of the Southern Ocean. It lists all the known Southern Ocean species and with illustrated diagrams provides a guide to their taxonomic identification. The document is available for download as a pdf from the provided URL.

This dataset is a document describing the Metazoan Zooplankton of the Southern Ocean. It lists all the known species and with illustrated diagrams provides a guide to their taxonomic identification. The document is available for download as a pdf from the provided URL.

Zooplankton were collected with a Rectangular Midwater Trawl (RMT 8+1 net) from 37 sampling sites on and near the Southern Kerguelen Plateau. Specimens of the euphausiid Thysanoessa macrura were selected for cohort analysis, based on lengths, and allometry (dry weights and lengths). Lipids were extracted from the animals to provide a lipid content (%) as a function of dry weight. A small number of individuals was examined further to produce profiles of the main fatty acids and fatty alcohols. Instantaneous growth rate experiments (IGR) were conducted onboard to determine growth rates of males, females and juveniles.

We studied the gut contents of four dominant copepod species (Calanoides acutus, Calanus propinquus, Calanus simillimus and Rhincalanus gigas) during the summer (2014-2015) along a latitudinal gradient (sampled every 5° between 40°S and 65°S) in the Indian sector of the SO. Diatoms were the most abundant food item found in the guts, comprising 24 of the 25 species found, and 15 were common to the four species of copepod studied. Diatoms accounted for the lowest proportion of the diet in the warmer, northern waters while all the large diatoms (e.g. Chaetoceros atlanticus, C. criophilus, C. dichaeta, Corethron spp.) were only found at 65oS. The most frequent species in the guts were the centric diatoms Thalassiosira spp. (4 to 57%) and the pennate diatoms Fragilariopsis kerguelensis (27 to 80%) and Trichoctoxon reinboldii (2 to 50%); proportions varied within a species across locations. These species were found at all sites examined, whereas some diatoms were specific to one copepod species: Asteromphalus spp. (in R. gigas), C. criophilus and C. dichaeta (in C. acutus), Nitzschia lecointei and N. sicula (in C. propinquus).

Zooplankton were collected during the winter-spring transition during two cruises of the Aurora Australis: SIPEX in 2007 and SIPEX II in 2012. The umbrella net was 2 metres long, 28 cm2 mouth area and mesh size of 100 um. The net was lowered through holes drilled through the pack ice and lowered to 100 m. It was pulled slowly by hand to the surface, closed and brought back through the ice hole. The contents were preserved in 5% buffered formaldehyde and examined under a Leica M12 in the laboratory. Species were identified to the lowest taxon possible.

This data set includes abundance and distribution of sea ice and water column micro-invertebrates. Data was collected form 8 stations over the course of the SIPEX 2 voyage. The data for this project consists of 2 separate collection regimes: 1. Ice cores - a number of ice were taken at stations 1-6, using a 15cm corer, they were sectioned and dissolved in filtered seawater before being fixed in formalin. At Station 6 sufficient biomass was present in the bottom 10cm of some cores to allow microscopic separation of copepod species, which were then frozen for stable isotope analysis (SIA). 2. Lazer optical plankton counter (LOPC) was deployed at stations 2 to 7 either from ice holes (IH) or from the trawl Deck (TD) to depths of 60m or 100m. These deployments were made throughout the day where possible and were accompanied by a 100um plankton net . Each deployment consisted of 2 drops for both the LOPC and the net. Collected plankton were filtered onto 50um mesh and backwashed into vials before being fixed in 5% formalin. All LOPC files .bin will require LOPC program manufactured by ODIM Rolls Royce Nova Scotia. These files are read to format .dat which maybe opened as a .txt file

This dataset contains scanned copies of the RMT and bottom trawl logs from Voyage 6 1990-91 (AAMBER2) of the Aurora Australis. This was primarily a marine science voyage. Surveys of krill, other zooplankton and pelagic fish were taken in Prydz Bay, Antarctica between January and February 1991. 177 midwater trawls were successfully completed at 59 stations. Midwater fish were sampled using an International Young Gadoid Pelagic Trawl (IYGPT). At each station, hauls were taken at depths of 20-30m, approximately halfway down the water column, and 20-30m above the bottom. At six stations, the lowest sample was duplicated using a light fitted to the net. Where samples were made off the shelf, standard depths of 20-30m, 400m, and 800m were fished. All hauls were of 30 minutes fishing time. Bottom trawls were made using a 35m headline length otter trawl fitted with 40cm diameter bobbin gear. A 2" mesh cod end liner was used to retain small fish. On both nets, a Simrad trawl surveillance sonar was used.

This dataset is a document describing the Scyphomedusae of the Southern Ocean. It lists all the known species and with illustrated diagrams provides a guide to their taxonomic identification. Distribution maps are given for each species. The document is available for download as a pdf from the provided URL.

Krill Ecology - Technical Reports and Systems Guides A series of documents detailing work completed and methods used at the Krill Aquarium located at the Australian Antarctic Division. Technical Report # Title and Author Technical Report 1. 26th January 1994. DAPI Epiflourescence Technique. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 2. 5th March 1995. Bag Culture - Cell Growth Count Protocol. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 3. 12th January 1996. Chemical 'Spiking' of Krill Aquarium Bio-filter T12. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 4. 24th June 1996. Cold Temperature Algal Bag Culture Methodology. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 5. 16th April 1997. Algal Bag Culture - Harvesting Method. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 6. 26th October 1999. Aquarium System Bulk Seawater Collection and Storage. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 7. 11th October 1999. Sodium Hypochlorite Treatment of Algal Bag Culture Filtration Unit. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 8. 18th October 1999. Feeding Krill - Algal Strains, Feeding Rate and Nutritional Values. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 9. 22nd November 1999. Krill Biology Section - Parental Algal Culture Maintenance. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 10. 10th April 2000. Krill Group Databases and Maintaining Daily Data Records. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 11. 11th May 2000. Making Up and Use of Iodine Solution as an Indicator of the Presence of Chlorine in Freshwater. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 12. 1st June 2000. Testing for Harmful Ammonia (NH3) in Aquarium Sea Water. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 13. 12th June 2000. Digitron Digilog 2088T Digital Temperature Logger/Gauge - Operating Instructions and Down-Loading Logged Data Guide. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 14. 27th June 2000. Krill Biology - Marine Science Support Shed Gear Storage. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 15. 15th October 2000. Making up of fe Growth Media Stock Solutions for Parental and Algal Bag Culture Production. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 16. 15th January 2001. Algal Bag Culture - Growth Rate Analysis. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 17. 19th July 2004. Protective Epoxy Coating of Onga Seawater Collection Fire Pump. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 18. 27th October 2004. New Krill Aquarium - Bulk Seawater Collection and Storage Logistics. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 19. 11th March 2005. New Krill Aquarium - Algal Bag Culture Filtration System. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 20. 6th April 2005. New Culture Cabinet Bag to Bag Inoculation Procedure. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 21. 17th June 2005. Agar Bacterial Plate Testing for Krill Algal Culture Stocks. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 22. 29th July 2004. New Algal Culture Cabinet - Bag Culture Setup Methodology. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 23. 24th May 2005. Protocol for Sterilization of Bag Culture Air Supply System. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 24. 30th May 2005. 200 litre tank Algal Batch Culture Setup. Author: P. M. Cramp. Australian Antarctic Division. Technical Report 25. 22nd June 2005. Making Up and Shaping Plastic Bags for Algal Culture. Author: P. M. Cramp. Australian Antarctic Division. Techincal Report 26. 19th December 2005. New Krill Aquarium - Algal Strains, Feeding Rates and Nutritional Values. Author: P. M. Cramp. Australian Antarctic Division.

Overview of the project and objectives: Sea-ice phytoplankton is significantly enriched in 13C (delta 13C-POC) compared to pelagic phytoplankton in adjacent open waters because of carbon limitation in the brine pockets and due to physiological properties such as the presence of Carbon Concentrating Mechanisms (CCM) and/or the uptake of bicarbonate (HCO3-). Melting of sea-ice with release of sea-ice phytoplankton occurs during the growth season, so these isotopically heavy particles, if sinking out of the surface waters, can be expected to be found deeper in the water column. One hypothesis is that the natural carbon isotopic signal of brassicasterol (phytosterol, mainly diatom indicator) in the south Antarctic Bottom Water (AABW), a water mass which is influenced by the Seasonal Ice Zone (SIZ), is enriched compared to northern deep waters signal due to an enhanced contribution of sea-ice diatoms. The objective of this dataset acquisition is to gain information on the delta 13C signal of brassicasterol in sea-ice diatoms and further estimate the contribution of sea-ice algae release in the Southern Ocean biological pump. In the course of the expedition, a second choice has been done to look at the presence of particulate barium in the sea-ice. In the open ocean, presence of particulate barium in the mesopelagic layer is an indicator of remineralisation process. The main idea is that marine snow composed of detritical organic matter (aggregates, faecal pellets, etc.) provides micro-environment favorable for precipitation of excess Barium or Baxs (total particulate Ba minus the lithogenic part; mainly constituted of barite crystals, BaSO4): is there such Baxs components in the sea-ice? Methodology and sampling strategy: Sampling strategy follows ice stations deployment via Bio ice-core type. Most of the time we worked close to / directly on the Trace Metal site following precautions concerning TM sampling (clean suits etc.). When we worked close to the TM site, precautions were not such important because we don't need the same drastic precautions for our own sampling. We work together because we want to propose a set of data which helps to characterize the system of functioning in close relation with TM availability (for that, sampling location have to be as close as possible). Ice melted from ice-core sections (see attached files for more details) is filtered on precombusted GF-F filters (0.7 microns porosity) and filters are stored at -20 degrees C. For particulate Barium sampling, same protocol but filtration on PC filters 0.4 microns, dry over night and store at ambient temperature. At home laboratory (VUB, Brussels, Belgium), sterols samples are analysed via Gas Chromatography - Mass Spectrometer (GC-MS) and Gas Chromatography-combustion column-Isotope Ratio Mass Spectrometer (GC-c-IRMS) after chemical treatment. Barium sample are analysed via Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES).