The impact of freeze-thaw cycling on a ZVI and inert medium was assessed using duplicated Darcy boxes subjected to 42 freeze-thaw cycles. This dataset consists of particle sizing during the decommissioning process of the experiment. Two custom built Perspex Darcy boxes of bed dimensions: length 362 mm, width 60 mm and height 194 mm were filled with a mixture of 5 wt% Peerless iron (Peerless Metal Powders and Abrasive, cast iron aggregate 8-50 US sieve) and 95 wt% glass ballotini ground glass (Potters Industries Inc. 25-40 US sieve). This ratio of media was selected to ensure that most aqueous contaminant measurements were above the analytical limit of quantification (LOQ) for feed solutions at a realistic maximum Antarctic metal contaminant concentration at a realistic field water flow rate. All solutions were pumped into and out of the Darcy boxes using peristaltic pumps and acid washed Masterflex FDA vitron tubing. Dry media was weighed in 1 kg batches and homogenised by shaking and turning end over end in a ziplock bag for 1 minute. To ensure that the media was always saturated, known amounts of Milli-Q water followed by the homogenised media were added to each box in approximately 1 cm layers. 20 mm of space was left at the top of the boxes to allow for frost heave and other particle rearrangement processes. On completion of freeze-thaw cycling and solution flow (refer to Statham 2014), an additional series of assessments was conducted. The media from between the entry weir and the first sample port was removed in five approximately 400 g samples of increasing depth. This procedure was repeated between the last sample port and the exit weir. These samples were left to dry in a fume cabinet before duplicated particle sizing using a Endcotts minor sieve shaker.

Microscopy imaging of live Antarctic krill using a Leica M205C dissecting stereo-microscope with a Leica DFC 450 camera and Leica LAS V4.0 software. Krill were held in a custom made 'krill trap', details provided in manuscript in section eight of this form. The data are available as a single video file. These data are part of Australian Antarctic Science (AAS) projects 4037 and 4050. Project 4037 - Experimental krill biology: Response of krill to environmental change The experimental krill research project is designed to focus on obtaining life history information of use in managing the krill fishery - the largest Antarctic fishery. In particular, the project will concentrate on studies into impacts of climate change on key aspects of krill biology and ecology. Project 4050 - Assessing change in krill distribution and abundance in Eastern Antarctica Antarctic krill is the key species of the Southern Ocean ecosystem. Its fishery is rapidly expanding and it is vulnerable to changes in climate. Australia has over a decade of krill abundance and distribution data collected off Eastern Antarctica. This project will analyse these datasets and investigate if krill abundance and distribution has altered over time. The results are important for the future management of the fishery, as well as understanding broader ecological consequences of change in this important species.

Metadata record for data from ASAC Project 2547 See the link below for public details on this project. Pue (greater than 90% as determined by SDS-PAGE) samples of nitrate reductase have been isolated from the Antarctic bacterium, Shewanella gelidimarina (ACAM 456T; Accession number U85907 (16S rDNA)). The protein is ~90 kDa (similar to nitrate reductase enzymes characterised from alternate bacteria) and stains positive in an in-situ nitrate reduction (native) assay technique. The protein may be N-terminal blocked, although further sequencing experiments are required to confirm this. This work is based upon phenotyped Antarctic bacteria (S. gelidimarina; S.frigidimarina) that was collected during other ASAC projects. (Refer: Psychrophilic Bacteria from Antarctic Sea-ice and Phospholipids of Antarctic sea ice algal communities new sources of PUFA [ASAC_708] and Biodiversity and ecophysiology of Antarctic sea-ice bacteria [ASAC_1012]). The download file contains 4 scientific papers produced from this work - one of these papers also contains a large set of accession numbers for data stored at GenBank.

This video is supplementary data for the publication entitled 'Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques'. The video is high resolution microscopy video of a live krill captured in the krill containment trap placed within the water bath. File size: 1.8 GB, 32 s duration. The optical microscopy was carried out using a Leica M205C dissecting stereomicroscope with a Leica DFC 450 camera and Leica LAS V4.0 software to collect high-resolution video. The experimental krill research project is designed to focus on obtaining life history information of use in managing the krill fishery - the largest Antarctic fishery. In particular, the project will concentrate on studies into impacts of climate change on key aspects of krill biology and ecology.

General description: The associated file contains sediment pigment data from the antFOCE project 4127. Units: all pigment data in ug/g, 0 = below detection limit of HPLC. Sample collection details: At the start and end of the antFOCE experiment, four sediment core samples were taken from inside and outside each chamber or open plot by divers. The top 1 cm of the cores was then removed and placed in the dark, first at -20ºC for 2 hours, then at -80ºC until analysis at the Australian Antarctic division. Pigment analysis Frozen samples were transported under liquid N2 to a freeze drier (Dynavac, model FD-5), in pre-chilled flasks with a small amount of liquid N2 added. Custom made plumbing fitted to the freeze drier enabled samples to be purged with N2 to prevent photo-oxidation up until solvent extraction. Prior to pigment extraction five 2 g stainless steel ball bearings were added to homogenise the freeze dried sediment. The samples were bead beaten for 1 minute (Biospec products). Subsamples (~0.05 g) were immediately transferred to cryotubes with 700 µl of dimethylformamide (DMF) for two hours. Samples were kept at -80ºC and under a safe light (IFORD 902) at all times. All pigment concentrations are standardised to sediment weight. Pigments were extracted with dimethylformamide (DMF 700 µl) over a two hour period at -20ºC. Zirconia beads, and 100 µl of Apo 8 and an internal standard were added to each sub-sample. After a two hour extraction, sub-samples were bead beaten for 20 seconds and then placed in a centrifuge with filter cartridge inserts for 14 minutes at 2500 rpm at -9ºC to separate the solvent from the sediment. The supernatant was transferred into to a vial and placed in a precooled rpHPLC autosampler. The rpHPLC system used is described in Hodgson et al. (1997). Pigment detection was at 435, 470 and 665 nm for all chlorophylls and carotenoids, with spectra from 300–700 nm being collected every 0.2 seconds. Pigment identification was carried out using a combination of rpHPLC and normal phase HPLC retention times, light absorbance spectra and reference standards (see Hodgson et al., 1997). These techniques assisted in the accurate identification of pigments and their derivatives to a molecular level and enabled several pigment derivatives to be analysed. The HPLC was previously calibrated with authentic standards and protocols outlined in SCOR (1988). Data set headers: (A)Treatment: Example code 4127_SOP7_6-1-15_PlotB_R1, = prodject code_Standard Operating Procedure(SOP) used to collect samples(see antFOCE parent file)_ Date_Chamber/plot(A,B,C,D)_replicate core within Chamber/plot(1,2,3) (B) BB carot= BB caroten, type of pigment detected by HPLC. See Wright, S.W., Jeffrey, S.W. and Mantoura, R.F.C. eds., 2005. Phytoplankton pigments in oceanography: guidelines to modern methods. Unesco Pub for more details. (C) Chl c1 = Chlorophyll derivatives see Wright, S.W., Jeffrey, S.W. and Mantoura, R.F.C. eds., 2005. Phytoplankton pigments in oceanography: guidelines to modern methods. Unesco Pub for more information. (D) Chl c2 = Chlorophyll derivatives see Wright, S.W., Jeffrey, S.W. and Mantoura, R.F.C. eds., 2005. Phytoplankton pigments in oceanography: guidelines to modern methods. Unesco Pub for more information. (E) Chl c3 = Chlorophyll derivative see Wright, S.W., Jeffrey, S.W. and Mantoura, R.F.C. eds., 2005. Phytoplankton pigments in oceanography: guidelines to modern methods. Unesco Pub for more information. (F) Chla = Chlorophyll a see Wright, S.W., Jeffrey, S.W. and Mantoura, R.F.C. eds., 2005. Phytoplankton pigments in oceanography: guidelines to modern methods. Unesco Pub for more information. (G) Ddx =Diadinoxanthin see Wright, S.W., Jeffrey, S.W. and Mantoura, R.F.C. eds., 2005. Phytoplankton pigments in oceanography: guidelines to modern methods. Unesco Pub for more information (H) dtx = Diatoxanthin pigment. see Wright, S.W., Jeffrey, S.W. and Mantoura, R.F.C. eds., 2005. Phytoplankton pigments in oceanography: guidelines to modern methods. Unesco Pub for more information (I) epi = Chlorophyll epimer pigment. see Wright, S.W., Jeffrey, S.W. and Mantoura, R.F.C. eds., 2005. Phytoplankton pigments in oceanography: guidelines to modern methods. Unesco Pub for more information. (j) Fuc = Fucoxanthin pigment. see Wright, S.W., Jeffrey, S.W. and Mantoura, R.F.C. eds., 2005. Phytoplankton pigments in oceanography: guidelines to modern methods. Unesco Pub for more information. (k) Gyro2 = Gyroxanthin pigment. see Wright, S.W., Jeffrey, S.W. and Mantoura, R.F.C. eds., 2005. Phytoplankton pigments in oceanography: guidelines to modern methods. Unesco Pub for more information. (L) Pras = Prasanthin pigment. see Wright, S.W., Jeffrey, S.W. and Mantoura, R.F.C. eds., 2005. Phytoplankton pigments in oceanography: guidelines to modern methods. Unesco Pub for more information. (m) Zea = Zeaxanthin pigment. see Wright, S.W., Jeffrey, S.W. and Mantoura, R.F.C. eds., 2005. Phytoplankton pigments in oceanography: guidelines to modern methods. Unesco Pub for more information. (n) Date = Samples taken at the start of antFOCE experiment or at the end (o) chamber = The antFOCE chamber (A,B,C,D) (p) Treatment = The associated pH level in chambers (Acidified ~7.8, Control ~8.2) (Q) Position = Samples were taken within chambers and outside chambers (outside, inside) (r) rep= Subsamples were taken within each chamber/position (R1=replicate one, R1-R4) Spatial coordinates: 66.311500 S, 110.514216 E Dates: between 1/12/2014 and 1/3/2015 Timezone:UTC+11

This metadata record was created in error and a DOI assigned to it before the error was noticed. The correct metadata record is available here: https://data.aad.gov.au/metadata/records/AAS_4015_Krill_Gonad_Transcriptome with the DOI doi:10.26179/5cd3c8fec9ad8.

Trace metal concentrations are reported in micrograms per gram of sediment in core C012-PC05 (64⁰ 40.517’ S, 119⁰ 18.072’ E, water depth 3104 m). Each sediment sample (100-200mg) was ground using a pestle and mortar and digested following an initial oxidation step (1:1 mixture of H2O2 and HNO3 acid) and open vessel acid on a 150 degree C hotplate using 2:5:1 mixture of concentrated distilled HCl, HNO3 and Baseline Seastar HF acid. After converting the digested sample to nitric acid, an additional oxidation step was performed with 1:1 mixture of concentrated distilled HNO3 and Baseline Seastar HClO4 acid. A 10% aliquot of the final digestion was sub-sampled for trace metal analyses. Trace metal concentrations were determined by external calibration using an ELEMENT 2 sector field ICP-MS from Thermo Fisher Scientific (Bremen, Germany) at Central Science Laboratory (University of Tasmania). The following elements were analysed in either low (LR) or medium resolution (MR): Sr88(LR), Y89(LR), Mo95(LR), Ag107(LR), Cd111(LR), Cs133(LR), Ba137(LR), Nd146(LR), Tm169(LR), Yb171(LR), Tl205(LR), Pb208(LR), Th232(LR), U238(LR), Na23(MR), Mg24(MR), Al27(MR), P31(MR), S32(MR), Ca42(MR), Sc45(MR), Ti47(MR), V51(MR), Cr52(MR), Mn55(MR), Fe56(MR), Co59(MR), Ni60(MR), Cu63(MR), Zn66(MR).

To quantify the dietary preferences and trophic level consumption of post-breeding adult female Antarctic fur seals (Arctocephalus gazella), we analysed the carbon:nitrogen composition of whiskers and blood samples from the females. Females were captured towards the end of the lactation period (March/April) and whiskers and a blood sample were collected at this time. Females were generally recaptured just prior to or after giving birth the following season and a further whisker and blood sample were collected at this time. Metadata for each individual include: Site, GLS ID, year, flipper tag number, season, sampling date, tissue type, whisker segment number, cumulative length along whisker of the segment, d15N, d13C, percentage N, percentage C and CN ratio.

Public Ocean acidification and warming are global phenomena that will impact marine biota through the 21st century. This project will provide urgently needed predictive information on the likely survivorship of benthic invertebrates in near shore Antarctic environments that is crucial for risk assessment of potential future changes to oceans. As oceans acidify carbonate saturation decreases, reducing the material required to produce marine skeletons. By examining the effects of increased ocean temperature and acidification on planktonic and benthic life stages of both calcifying and non-calcifying ecologically important organisms, predictions can be made on the potential vulnerability of marine biota to climatic change. Project Objectives: This project aims to deliver one of the first assessments of the impacts that ocean warming and acidification through rising CO2 levels will have on Antarctic benthic marine invertebrates and of the adaptive capacity of common Antarctic biota to climate change. The developmental success of species that have a skeleton will be compared to those that do not under controlled conditions of increased sea water temperature and CO2. A comparison of the responses and sensitivity of developmental stages of calcifiers (echinoids, bivalves) and non-calcifiers (asteroids) to elevated CO2 and temperature will generate much needed empirical data for assessment of risk and adaptive capacity of Antarctica's marine biota and will enable predictions of how benthic invertebrates will fare with respect to climate change scenarios. This dataset addresses objective 3, and part of objective 5: 3 - compare the dynamics of biomineralisation with respect to the elemental composition in response to increased temperature and CO2 in species with aragonite and calcite exoskeletons (bivalves) and porous high magnesium calcite endoskeletons (echinoids) to assess the potential for an in-built adaptive response in calcification 5 - compare biomineralisation and elemental signatures in skeletons in larvae of Antarctic molluscs and echinoderms under climate change scenarios with that determined for related species at lower latitudes to assess the relative sensitivity and vulnerability of Antarctic biota. These data are XRD - x-ray diffractometry of the skeleton to provide data on the element content of the calcite mineral. The Mg2+ level is of interest because the higher the Mg content the more vulnerable the skeleton is to ocean acidification. Wt% MgCO3 in the calcite sample - for each category; test (- "shell"); Spines (-= lg primary spines) and secondary spines

This data describes the cellular metal concentrations of Phaeocystis antarctica and Cryothecomonas armigera following exposure to metals singly and in mixtures in laboratory studies. Microalgae were cultured in 80 mL of filtered (less than 0.45 um) seawater and low concentrations of nutrients supplemented with metal stocks to give a range of single and mixture exposures to the metals cadmium, copper, nickel, lead, and zinc. The cellular accumulation and partitioning are used to explain the metal's toxicity (cellular metal fractions are compared to the toxicity data provided in 10.4225/15/5ae93ff723ff8) and assess the risk bioaccumulation of metals to Antarctic marine microalgae may pose in the Southern Ocean food web.