This video is supplementary data for the publication entitled 'Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques'. The video is high resolution microscopy video of a live krill captured in the krill containment trap placed within the water bath. File size: 1.8 GB, 32 s duration. The optical microscopy was carried out using a Leica M205C dissecting stereomicroscope with a Leica DFC 450 camera and Leica LAS V4.0 software to collect high-resolution video. The experimental krill research project is designed to focus on obtaining life history information of use in managing the krill fishery - the largest Antarctic fishery. In particular, the project will concentrate on studies into impacts of climate change on key aspects of krill biology and ecology.

These aerial survey data of southern right whales (Eubalaena australis) off southern Australia were collected in August 2018. Such annual flights in winter/spring between Cape Leeuwin (Western Australia) and Ceduna (South Australia) have now been conducted over a 26-year period 1993-2018. These surveys have provided evidence of a population trend of around 6% per year, and a current (at 2014) population size of approximately 2300 of what has been regarded as the 'western' Australian right whale subpopulation. With estimated population size in the low thousands, it is presumed to be still well below carrying capacity. No trend information is available for the 'eastern' subpopulation of animals occurring around the remainder of the southern Australian Coast, to at least as far as Sydney, New South Wales and the populations size is relatively small, probably in the low hundreds. A lower than expected 'western' count in 2015 gives weak evidence that the growth rate may be starting to show signs of slowing, though an exponential increase remains the best description of the data. If the low 2015 count is anomalous, future counts may be expected to show an exponential increase, but if it is not, modelling growth as other than simple exponential may be useful to explore in future.

This is a local copy of a metadata record and dataset stored at Dryad. This local copy is maintained in order to provide a link to the originating Australian Antarctic program project. See the link to the Dryad site at the provided URL for full details on this data set. Age is a fundamental aspect of animal ecology, but is difficult to determine in many species. Humpback whales exemplify this as they have a lifespan comparable to humans, mature sexually as early as four years and have no reliable visual age indicators after their first year. Current methods for estimating humpback age cannot be applied to all individuals and populations. Assays for human age have recently been developed recently based on age-induced changes in DNA methylation of specific genes. We used information on age-associated DNA methylation in human and mouse genes to identify homologous gene regions in humpbacks. Humpback skin samples were obtained from individuals with a known year of birth and employed to calibrate relationships between cytosine methylation and age. Seven of 37 cytosines assayed for methylation level in humpback skin had significant age-related profiles. The three most age-informative cytosine markers were selected for a humpback epigenetic age assay. The assay has an R2 of 0.787 (p = 3.04e-16) and predicts age from skin samples with a standard deviation of 2.991 years. The epigenetic method correctly determined which of parent-offspring pairs is the parent in more than 93% of cases. To demonstrate the potential of this technique, we constructed the first modern age profile of humpback whales off eastern Australia and compared the results to population structure five decades earlier. This is the first epigenetic age estimation method for a wild animal species and the approach we took for developing it can be applied to many other non model organisms.

These aerial survey data of southern right whales (Eubalaena australis) off southern Australia were collected in September 2015. Such annual flights in winter/spring between Cape Leeuwin (Western Australia) and Ceduna (South Australia) have now been conducted over a 23-year period 1993-2015. These surveys have provided evidence of a population trend of around 6% per year, and a current (at 2014) population size of approximately 2300 of what has been regarded as the 'western' Australian right whale subpopulation. With estimated population size in the low thousands, it is presumed to be still well below carrying capacity. No trend information is available for the 'eastern' subpopulation of animals occurring around the remainder of the southern Australian Coast, to at least as far as Sydney, New South Wales and the populations size is relatively small, probably in the low hundreds. A lower than expected 'western' count in 2015 gives weak evidence that the growth rate may be starting to show signs of slowing, though an exponential increase remains the best description of the data. If the low 2015 count is anomalous, future counts may be expected to show an exponential increase, but if it is not, modelling growth as other than simple exponential may be useful to explore in future. A data update was provided in August, 2020 to correct some incorrectly given longitude values.

These aerial survey data of southern right whales (Eubalaena australis) off southern Australia were collected in August 2017. Such annual flights in winter/spring between Cape Leeuwin (Western Australia) and Ceduna (South Australia) have now been conducted over a 25-year period 1993-2017. These surveys have provided evidence of a population trend of around 6% per year, and a current (at 2014) population size of approximately 2300 of what has been regarded as the 'western' Australian right whale subpopulation. With estimated population size in the low thousands, it is presumed to be still well below carrying capacity. No trend information is available for the 'eastern' subpopulation of animals occurring around the remainder of the southern Australian Coast, to at least as far as Sydney, New South Wales and the populations size is relatively small, probably in the low hundreds. A lower than expected 'western' count in 2015 gives weak evidence that the growth rate may be starting to show signs of slowing, though an exponential increase remains the best description of the data. If the low 2015 count is anomalous, future counts may be expected to show an exponential increase, but if it is not, modelling growth as other than simple exponential may be useful to explore in future

RNA was extracted from pooled gonad tissues and tails of five sexually mature males and females, respectively, originating from the krill aquarium at the AAD in Tasmania, Australia. For RNA extractions, RNeasy mini kits (QIAGEN) were used and total RNA (8 micrograms each) was sent to Geneworks, South Australia (www.geneworks.com.au), for Illumina TruSeq 75 bp paired-end sequencing in two technical replica. Reads Yield Total Yield Krill_Male_sex_a_read1_sequence.txt 8,120,993 609,074,475 bases 1,218,148,950 bases Krill_Male_sex_a_read2_sequence.txt 8,120,993 609,074,475 bases Krill_Male_sex_b_read1_sequence.txt 10,465,586 784,918,950 bases 1,569,837,900 bases Krill_Male_sex_b_read2_sequence.txt 10,465,586 784,918,950 bases Krill_Male_tissue_a_read1_sequence.txt 7,867,804 590,085,300 bases 1,180,170,600 bases Krill_Male_tissue_a_read2_sequence.txt 7,867,804 590,085,300 bases Krill_Male_tissue_b_read1_sequence.txt 10,956,251 821,718,825 bases 1,793,118,450 bases Krill_Male_tissue_b_read2_sequence.txt 10,956,251 821,718,825 bases Krill_Female_sex_read1a_sequence.txt 29,447,654 2,208,574,050 bases 4,417,148,100 bases Krill_Female_sex_read2a_sequence.txt 29,447,654 2,208,574,050 bases Krill_Female_sex_read1b_sequence.txt 18,223,515 1,366,763,625 bases 2,733,527,250 bases Krill_Female_sex_read2b_sequence.txt 18,223,515 1,366,763,625 bases The insert size for these libraries is approx 160bp.

This data record has been compiled for a statistical methods study, conducted by Abigael Proctor as part of her PhD research in 2018. The data in this record have been used to showcase a new statistical method for determining no effect concentration (NEC). The study uses the data in this record to compare NEC and LCx estimates for copper in four Antarctic marine invertebrate species. The data associated with this record are a subset of four existing larger datasets: 1. amphipod: AAS_2933_Orchomenella_pinguides_Sensitivity_metals_Davis_2010-11 2. copepod: AAS_4100_Toxicity_Copepods 3. gastropod: AAS_2933_MetaToxicityMarine_JuvenileGastropods_Kingston2007 4. ostracod: AAS_2933_MetalToxicityMarine_BrownOstracods_Kingston2007 Subset details are described in the excel file provided.

We use RNA sequencing to investigate which genetic/physiological pathways in Antarctic krill are affected by increased CO2 levels. We carried out larval CO2 exposure experiments in March 2012 at the AAD aquarium. Two developmental stages were used (Calyptopis I and Furcilia V) and three CO2 levels (control, 1000 and 2000 ppm). These were short term experiments (2 days) - since initial longer experiments starting with fertilized eggs resulted in differences in developmental stages between treatments and control which could confound the data. RNA was extracted from larvae and high-throughput RNA sequencing (RNA-seq) was carried out on 6 samples (2 stages * 3 treatments). Sequencing was carried out on an Illumina sequencer (Genome Analyzer II). We collected ~ 60 million sequence reads per sample (Data in FASTA format each read gives 100 base pairs of sequence), so a total of ~360 million reads (36 billion bp of data).

Satellite tracks of humpback whales tagged off the Sunshine Coast, Queensland, Australia and tracked to Antarctic feeding grounds. Data can be found here: https://data.aad.gov.au/aadc/argos/display_campaign.cfm?campaign_id=87 Satellite tags were deployed on adult humpback whales with a modified version of the Air Rocket Transmitter System (ARTS, Restech) and a purpose-designed projectile carrier at a pressure of 7.5 – 10 bar. A custom-designed, 80mm anchor section is attached to a stainless steel cylindrical housing containing a location-only transmitter (SPOT-5 by Wildlife Computers, Redmond, Washington, USA and Kiwisat 202 Cricket by Sirtrack, Havelock North, New Zealand). This superseded anchor design resulted in the anchor section disarticulating upon deployment in order to achieve improved tag retention times while minimising impact. The tags were sterilised with ethylene oxide prior to deployment and implanted up to 290mm into the skin, blubber, interfacial layers and outer muscle mass of the whale. Tags were programmed to transmit to the Argos satellite system at various duty cycles and repetition rates for a maximum of 720 transmissions per day. These transmissions are relayed to processing centres which calculate the transmitter’s location by measuring the Doppler Effect on transmission frequency.

Annual aerial surveys of southern right whales have been conducted off the southern Australian coast, between Cape Leeuwin (W.A.) and Ceduna (S.A.) over a 28 year period between 1993 and 2020, to monitor the recovery of this species following commercial whaling. We conducted an aerial survey of southern right whales between the 20th and 24th August 2020, to continue these annual series of surveys and inform the long-term population trend. The comparable count for the 2020 survey utilised the maximum count for each leg and incorporated a correction for the unsurveyed area between Head of the Bight to Ceduna due to the inability to cover whole survey as a result of COVID-19 restrictions between State borders. This resulted in 384 individuals, consisting of 156 cows accompanied by calves of the year and 72 unaccompanied adults. Of these, 126 images of individual whales have been selected for photo-identification matching. This is a significant decrease in overall sightings that has not been observed for over 13 years when compared to long term trend data for the population; last seen in 2007 (N = 286 individuals). The subsequent population estimate for the Australian ‘western’ subpopulation is 2,585 whales, which is also a significant decrease in estimated population size from 3,164 in 2019 to 2,585 in 2020. The extremely low number of unaccompanied adults (N = 68) had the greatest impact on the overall number of sightings in 2020, and is the lowest number sighted since 1993 (N = 47). Previous surveys in 2007 and 2015 have been noted as years of low whale counts that had been deemed anomalous years, although the low numbers from this survey questions this and may suggest the 3-year female breeding cycle is becoming more unpredictable. Considerable inter-annual variation in whale numbers, and cycles in population growth, makes it difficult to detect consistent and reliable changes in abundance from one year to the next, or even over longer periods of time. This severely inhibits our ability to identify immediate threats to the population and strongly supports continued annual population surveys.