These are the scanned electronic copies of field and lab books used at Davis Station, and Kingston between 2009 and 2011 as part of ASAC (AAS) project 3217 - Environmental assessment of Davis sewage treatment plant up-grade.

Sediment Recruitment Experiment 4 (SRE4) was a large, long term (5 year) field experiment run at Casey Station (from 2001 to 2006) testing the effects of 4 different hydrocarbons on marine sediment ecosystems. Four different types of hydrocarbons were individually mixed with defaunated marine sediments and deployed in trays on the seabed at O'Brien Bay-1. Trays were collected after deployment periods of 5 weeks, 56 weeks, 62 weeks, 2 years and 5 years. In addition there was a bioturbation treatment using the burrowing urchin Abatus (at 56 weeks only). Samples were collected from 4 replicate trays of each treatment at each sampling time. Analyses were done of sediment hydrocarbon chemistry, microbial communities, meiofaunal communities, macrofaunal communities and diatom communities. The hydrocarbon treatments were: a synthetic Mobil lubricating oil; the same Mobil lubricating oil after 125? hours use in a vehicle engine; a Fuchs synthetic lubricating oil marketed as highly biodegradable; and Special Antarctic Blend diesel fuel (SAB). A control uncontaminated sediment treatment was used for comparison.

Sediment Recruitment Experiment 4 (SRE4) was a large, long term (5 year) field experiment run at Casey Station (from 2001 to 2006) testing the effects of 4 different hydrocarbons on marine sediment ecosystems. Four different types of hydrocarbons were individually mixed with defaunated marine sediments and deployed in trays on the seabed at O'Brien Bay-1. Trays were collected after deployment periods of 5 weeks, 56 weeks, 62 weeks, 2 years and 5 years. In addition there was a bioturbation treatment using the burrowing urchin Abatus (at 56 weeks only). Samples were collected from 4 replicate trays of each treatment at each sampling time. Analyses were done of sediment hydrocarbon chemistry, microbial communities, meiofaunal communities, macrofaunal communities and diatom communities. The hydrocarbon treatments were: a synthetic Mobil lubricating oil; the same Mobil lubricating oil after 125? hours use in a vehicle engine; a Fuchs synthetic lubricating oil marketed as highly biodegradable; and Special Antarctic Blend diesel fuel (SAB). A control uncontaminated sediment treatment was used for comparison.

Sediment Recruitment Experiment 4 (SRE4) was a large, long term (5 year) field experiment run at Casey Station (from 2001 to 2006) testing the effects of 4 different hydrocarbons on marine sediment ecosystems. Four different types of hydrocarbons were individually mixed with defaunated marine sediments and deployed in trays on the seabed at O'Brien Bay-1. Trays were collected after deployment periods of 5 weeks, 56 weeks, 62 weeks, 2 years and 5 years. In addition there was a bioturbation treatment using the burrowing urchin Abatus (at 56 weeks only). Samples were collected from 4 replicate trays of each treatment at each sampling time. Analyses were done of sediment hydrocarbon chemistry, microbial communities, meiofaunal communities, macrofaunal communities and diatom communities. The hydrocarbon treatments were: a synthetic Mobil lubricating oil; the same Mobil lubricating oil after 125? hours use in a vehicle engine; a Fuchs synthetic lubricating oil marketed as highly biodegradable; and Special Antarctic Blend diesel fuel (SAB). A control uncontaminated sediment treatment was used for comparison.

Sediment Recruitment Experiment 4 (SRE4) was a large, long term (5 year) field experiment run at Casey Station (from 2001 to 2006) testing the effects of 4 different hydrocarbons on marine sediment ecosystems. Four different types of hydrocarbons were individually mixed with defaunated marine sediments and deployed in trays on the seabed at O'Brien Bay-1. Trays were collected after deployment periods of 5 weeks, 56 weeks, 62 weeks, 2 years and 5 years. In addition there was a bioturbation treatment using the burrowing urchin Abatus (at 56 weeks only). Samples were collected from 4 replicate trays of each treatment at each sampling time. Analyses were done of sediment hydrocarbon chemistry, microbial communities, meiofaunal communities, macrofaunal communities and diatom communities. The hydrocarbon treatments were: a synthetic Mobil lubricating oil; the same Mobil lubricating oil after 125 hours use in a vehicle engine; a Fuchs synthetic lubricating oil marketed as highly biodegradable; and Special Antarctic Blend diesel fuel (SAB). A control uncontaminated sediment treatment was used for comparison.

Untreated, macerated wastewater effluent has been discharged to the sea at Davis Station since 2005, when the old wastewater treatment infrastructure was removed. This environmental assessment was instigated to guide the choice of the most suitable wastewater treatment facility at Davis. The assessment will support decisions that enable Australia to meet the standards set for the discharge of wastewaters in Antarctica in national legislation (Waste Management Regulations of the Antarctic Treaty Environmental Protection Act - ATEP) and to meet international commitments (the Madrid Protocol) and to meet Australia's aspirations to be a leader in Antarctic environmental protection. The overall objective was to provide environmental information in support of an operational infrastructure project to upgrade wastewater treatment at Davis. This information is required to ensure that the upgrade satisfies national legislation (ATEP/Waste Management Regulations), international commitments (the Madrid Protocol) and maintain the AAD's status as an international leader in environmental management. The specific objectives were to: 1. Wastewater properties: Determine the properties of discharged wastewater (contaminant levels, toxicity, microbiological hazards) as the basis for recommendations on the required level of treatment and provide further consideration of what might constitute adequate dilution and dispersal for discharge to the nearshore marine environment 2. Dispersal and dilution characteristics of marine environment: Assess the dispersing characteristics of the immediate nearshore marine environment in the vicinity of Davis Station to determine whether conditions at the existing site of effluent discharge are adequate to meet the ATEP requirement of initial dilution and rapid dispersal. 3. Environmental impacts: Describe the nature and extent of impacts to the marine environment associated with present wastewater discharge practices at Davis and determine whether wastewater discharge practices have adversely affected the local environment. 4. Evaluate treatment options: Evaluate the different levels of treatment required to mitigate and/or prevent various environmental impacts and reduce environmental risks.

Sediment Recruitment Experiment 4 (SRE4) was a large, long term (5 year) field experiment run at Casey Station (from 2001 to 2006) testing the effects of 4 different hydrocarbons on marine sediment ecosystems. Four different types of hydrocarbons were individually mixed with defaunated marine sediments and deployed in trays on the seabed at O'Brien Bay-1. Trays were collected after deployment periods of 5 weeks, 56 weeks, 62 weeks, 2 years and 5 years. In addition there was a bioturbation treatment using the burrowing urchin Abatus (at 56 weeks only). Samples were collected from 4 replicate trays of each treatment at each sampling time. Analyses were done of sediment hydrocarbon chemistry, microbial communities, meiofaunal communities, macrofaunal communities and diatom communities. The hydrocarbon treatments were: a synthetic Mobil lubricating oil; the same Mobil lubricating oil after 125? hours use in a vehicle engine; a Fuchs synthetic lubricating oil marketed as highly biodegradable; and Special Antarctic Blend diesel fuel (SAB). A control uncontaminated sediment treatment was used for comparison.

Sediment Recruitment Experiment 4 (SRE4) was a large, long term (5 year) field experiment run at Casey Station (from 2001 to 2006) testing the effects of 4 different hydrocarbons on marine sediment ecosystems. Four different types of hydrocarbons were individually mixed with defaunated marine sediments and deployed in trays on the seabed at O'Brien Bay-1. Trays were collected after deployment periods of 5 weeks, 56 weeks, 62 weeks, 2 years and 5 years. In addition there was a bioturbation treatment using the burrowing urchin Abatus (at 56 weeks only). Samples were collected from 4 replicate trays of each treatment at each sampling time. Analyses were done of sediment hydrocarbon chemistry, microbial communities, meiofaunal communities, macrofaunal communities and diatom communities. The hydrocarbon treatments were: a synthetic Mobil lubricating oil; the same Mobil lubricating oil after 125? hours use in a vehicle engine; a Fuchs synthetic lubricating oil marketed as highly biodegradable; and Special Antarctic Blend diesel fuel (SAB). A control uncontaminated sediment treatment was used for comparison.

Untreated, macerated wastewater effluent has been discharged to the sea at Davis Station since 2005, when the old wastewater treatment infrastructure was removed. This environmental assessment was instigated to guide the choice of the most suitable wastewater treatment facility at Davis. The assessment will support decisions that enable Australia to meet the standards set for the discharge of wastewaters in Antarctica in national legislation (Waste Management Regulations of the Antarctic Treaty Environmental Protection Act - ATEP) and to meet international commitments (the Madrid Protocol) and to meet Australia's aspirations to be a leader in Antarctic environmental protection. The overall objective was to provide environmental information in support of an operational infrastructure project to upgrade wastewater treatment at Davis. This information is required to ensure that the upgrade satisfies national legislation (ATEP/Waste Management Regulations), international commitments (the Madrid Protocol) and maintain the AAD's status as an international leader in environmental management. The specific objectives were to: 1. Wastewater properties: Determine the properties of discharged wastewater (contaminant levels, toxicity, microbiological hazards) as the basis for recommendations on the required level of treatment and provide further consideration of what might constitute adequate dilution and dispersal for discharge to the nearshore marine environment 2. Dispersal and dilution characteristics of marine environment: Assess the dispersing characteristics of the immediate nearshore marine environment in the vicinity of Davis Station to determine whether conditions at the existing site of effluent discharge are adequate to meet the ATEP requirement of initial dilution and rapid dispersal. 3. Environmental impacts: Describe the nature and extent of impacts to the marine environment associated with present wastewater discharge practices at Davis and determine whether wastewater discharge practices have adversely affected the local environment. 4. Evaluate treatment options: Evaluate the different levels of treatment required to mitigate and/or prevent various environmental impacts and reduce environmental risks.

Sediment Recruitment Experiment 4 (SRE4) was a large, long term (5 year) field experiment run at Casey Station (from 2001 to 2006) testing the effects of 4 different hydrocarbons on marine sediment ecosystems. Four different types of hydrocarbons were individually mixed with defaunated marine sediments and deployed in trays on the seabed at O'Brien Bay-1. Trays were collected after deployment periods of 5 weeks, 56 weeks (one year), 62 weeks, 2 years and 5 years. In addition there was a bioturbation treatment using the burrowing urchin Abatus (at 56 weeks only). Samples were collected from 4 replicate trays of each treatment at each sampling time. Analyses were done of sediment hydrocarbon chemistry, microbial communities, meiofaunal communities, macrofaunal communities and diatom communities. The hydrocarbon treatments were: a synthetic Mobil lubricating oil; the same Mobil lubricating oil after 125 hours use in a vehicle engine; a Fuchs synthetic lubricating oil marketed as highly biodegradable; and Special Antarctic Blend diesel fuel (SAB). A control uncontaminated sediment treatment was used for comparison. A randomised block design was used with a tray of each treatment in 24 blocks (5 trays per block). Four blocks were sampled at each time (4 trays of each treatment). Meiofauna Two replicate cores were taken from each tray at each time. Copepods were identified to family. Nematodes were identified to genus. Data is from one, two and five years of the experiment. At one year there is only one replicate per tray for copepod data (a total of 4 replicates per treatment).