We studied the gut contents of four dominant copepod species (Calanoides acutus, Calanus propinquus, Calanus simillimus and Rhincalanus gigas) during the summer (2014-2015) along a latitudinal gradient (sampled every 5° between 40°S and 65°S) in the Indian sector of the SO. Diatoms were the most abundant food item found in the guts, comprising 24 of the 25 species found, and 15 were common to the four species of copepod studied. Diatoms accounted for the lowest proportion of the diet in the warmer, northern waters while all the large diatoms (e.g. Chaetoceros atlanticus, C. criophilus, C. dichaeta, Corethron spp.) were only found at 65oS. The most frequent species in the guts were the centric diatoms Thalassiosira spp. (4 to 57%) and the pennate diatoms Fragilariopsis kerguelensis (27 to 80%) and Trichoctoxon reinboldii (2 to 50%); proportions varied within a species across locations. These species were found at all sites examined, whereas some diatoms were specific to one copepod species: Asteromphalus spp. (in R. gigas), C. criophilus and C. dichaeta (in C. acutus), Nitzschia lecointei and N. sicula (in C. propinquus).

Metadata record for data from ASAC Project 1101 See the link below for public details on this project. ---- Public Summary from Project ---- Most of our knowledge of the Antarctic marine ecosystems comes from summer surveys. There are very few observations of this ecosystem in winter and there is a fundamental lack of knowledge of understanding of even basic questions such as 'what is there?' and 'what's it doing?'. The proposed visit to the sea ice zone in winter is a rare opportunity to conduct observations on phytoplankton, krill, birds, seals and whales, so that we can begin to understand the biological processes that go on in winter. Data for this project were intended to be collected on a 1998 winter voyage of the Aurora Australis, but a fire on board meant that the voyage had to return to port before work could be carried out. Data were then collected the following year during a 1999 winter voyage of the Aurora Australis (IDIOTS), which ran from July to September. Data attached to this metadata record, include protist, bacteria and virus data collected from the Mertz Glacier region. The purpose of this research was to quantify and identify the different assemblages of phytoplankton, change in virus populations and the number of live/dead bacteria between Tasmania and the polynya, within the polynya and sea ice. A variety of methods were used including HPLC, light and fluorescent microscopy and the collection of samples for subsequent analysis upon return to the Antarctic Division. More information is available in the download file. The samples were collected by Rick van den Enden.

This data set was collected during an ocean acidification mesocosm experiment performed at Davis Station, Antarctica during the 2014/15 summer season. It includes: - description of methods for all data collection and analyses. - diatom cell volume - bulk silicification - species specific silicification via fluorescence microscopy - bulk community Fv/Fm on day 12 - single-cell PAM fluorometry data (maximum quantum yield of PSII: Fv/Fm) A natural community of Antarctic marine microbes from Prydz Bay, East Antarctica were exposed to a range of CO2 concentrations in 650 L minicosms to simulate possible future ocean conditions up to the year ~2200. Diatom silica precipitation rates were examined at CO2 concentrations between 343 to 1641 micro atm, measuring both the total diatom community response and that of individual species, to determine whether ocean acidification may influence future diatom ballast and therefore alter carbon and silica fluxes in the Southern Ocean. Described and analysed in: Antarctic diatom silicification diminishes under ocean acidification (submitted for review) Methods described in: Antarctic diatom silicification diminishes under ocean acidification (submitted for review) Location: Prydz bay, Davis Station, Antarctica (68 degrees 35'S, 77 degrees 58' E) Date: Summer 2014/2015 Worksheet descriptions: Bulk silicification - raw data Measured total and incorporated biogenic silica using spectrophotometer for all tanks on day 12 after 24 h incubation with PDMPO - raw data Bulk Fv/Fm - dark-adapted maximum quantum efficiency of PSII (Fv/Fm) on whole community - raw data Measured Fv/Fm of individual cells from 3 mesocosm tanks. Single-cell silicificiation, Fluorescence microscopy - raw data Measured autofluorescence and PDMPO fluorescence of individual diatoms from 6 mesocosm tanks Single-cell PAM, dark-adapted maximum quantum efficiency of PSII (Fv/Fm) - raw data Measured Fv/Fm of individual cells from 3 mesocosm tanks. Cell volume Calculated cell volume (um3) of 7 species from minicosm tanks 1 and 6 - raw data Abbreviations: Fv/Fm Maximum quantum yield of PSII PDMPO 2-(4-pyridyl)-5-((4-(2-dimethylaminoethylaminocarbamoyl)methoxy)phenyl)oxazole Tant Thalassiosira antarctica DiscLg Large Discoid centric diatoms Stella Stellarima microtrias Chaeto Chaetoceros spp. Prob Proboscia truncata Pseu Pseudonitzschia turgiduloides FragLg Fragilariopsis cylindrus / curta Centric Large Discoid centric diatoms LargeThalassiosira Large Discoid centric diatoms

The data set includes information relevant for the study and description of sea-ice bacteria contains the following dataset subgroups and is organised by REFERENCE number. 1) Isolation data: strain designations (e.g. culture collection names are indicated for type cultures); media used for isolation and routine cultivation; temperature used for incubation; any special conditions (e.g. enrichment conditions) used for isolation; isolation site and type (e.g. sea-ice); availability of the indicated strain from the chief investigator (J. Bowman) 2) Phenotypic data: Includes morphological, physiological and biochemical tests performed. Details on how these were performed are indicated in the relevant reference. 3) Growth/temperature data: data for temperature related growth curves are given where available. Methods are indicated in the associated reference. 4) Fatty acid/chemotaxonomy data: fatty acid and other related data are given where available. Methods are indicated in the associated reference. 5) Genotypic data: data for DNA-guanosine/cytosine-content and genomic DNA:DNA hybridization are shown where available. Methods are indicated in the associated reference. 6) Phylogenetic data: data for sequences are cross-referenced to the GenBank database. In some cases, aligned sequence datasets are available in FASTA format and can be viewed in the programs BIOEDIT (www.mbio.ncsu.edu/BioEdit/bioedit.html) or CLUSTAL W (www.ebi.ac.uk/clustalw). 7) Other related published references which are useful or relevant to the dataset e.g. related sequences published subsequent to the ASAC study

Antarctic sediments and sea-ice are important regulators in global biogeochemical and atmospheric cycles. These ecosystems contain a diverse range of bacteria whose biogeochemical roles remains largely unknown and which inhabit what are continually low temperature habitats. An integrated molecular and chemical approach will be used to investigate the coupling of microbial biogeochemical processes with community structure and cold adaptation within coastal Antarctic marine sediments and within sea-ice. Overall the project expects to make an important contribution to our understanding of biological processes within low temperature habitats. DATA SET ORGANISATION: The dataset is organised on the basis of publication and is organised on the basis of the following sections: 1. SEDIMENT SAMPLES and ISOLATES Samples collected are described in terms of location, type and where data were obtained chemical features. The designation, source, media used for cultivation and isolation and availability of sediment and other related isolates are provided. Samples included are from the following locations: Clear Lake, Pendant Lake, Scale Lake, Ace Lake, Burton Lake, Ekho Lake, Organic Lake, Deep lake and Taynaya Bay (Burke Basin), Vestfold Hills region; and the Mertz Glacier Polynya region. 2. BIOMASS and ENZYME ACTIVITY DATA Biomass, numbers and extracellular enzyme activity data are provided for Bacteria and Archaea populations from Mertz Glacier Polynya shelf sediments. 3. FATTY ACID and TETRAETHER LIPID DATA Phospholipid and tetraether lipid data are provided for Mertz Glacier Polynya shelf sediments. Whole cell fatty acid data are provided for various bacterial isolates described officially as new genera or species. 4. RNA HYBRIDISATION DATA RNA hybridisation data for Mertz Glacier Polynya sediment samples is provided, including data for oligonucleotide probes specifc for total Bacteria, Archaea, the Desulfosarcina group (class Deltaproteobacteria, sulfate reducing bacterial clade), phylum Planctomycetes, phylum Bacteroidetes (Cytophaga-Flavobacterium-Bacteroides), class Gammaproteobacteria, sulfur-oxidizing and related bacteria (a subset of class Gammaproteobacteria) and Eukaryota. 5. PHYLOGENETIC DATA 16S rRNA gene sequence data are indicated including aligned datasets for three clone libraries derived from the Mertz Glacier Polynya including GenBank accession numbers. Sequence accession numbers are provided for Vestfold Hills lake sediment samples. In addition GenBank numbers are provided for denaturing gradient gel electrophoresis band sequence data from Mertz Glacier Polynya shelf sediment. Other forms of this DGGE data (banding profile analysis) are available in reference Bowman et al. 2003 (AAD ref 10971).