This metadata record contains the results from bioassays conducted to show the response of larval Antarctic Sterechinus neumayeri sea urchins to contamination from combinations if IFO 180 fuel and the fuel dispersant Slickgone NS. AAS project 4142. Experiments used an intermediate grade Fuel Oil (IFO 180) and an internationally approved fuel dispersant, Slickgone NS, produced by Dasic International LTD. Treatments included a physically dispersed treatment of IFO 180 only, a chemically dispersed treatment of IFO 180 treated with Slickgone NS and a Slickgone NS only treatment to determine the toxicity of the dispersant. Treatments were experimentally mixed using a magnetic stirrer to combine treatment substances and filtered seawater (FSW) in temperature-controlled cabinets at 0oC to create a Water Accommodated Fraction (WAF). WAFs were produced in 2 L and 5 L glass aspirator bottles following the methods of Singer, Aurand et al. (2000) with adaptations by Barron and Ka'aihue (2003) and Kostzakoulakis (chemistry section, project 4142) stirring for 42 h with a settling time of 6 h. WAF treatments used concentrations of 100%, 50%, 20% and 10%, CEWAF and dispersant only treatments used concentrations of 10%, 5%, 1% and 0.1%. Toxicity tests were conducted in temperature-controlled cabinets at 0 oC using uncapped, forty-millilitre glass headspace vials, each containing 15.5 ml of test solution and 0.5 ml of embryo suspension. Fertilisation methods followed standard procedures for Sterechinus neumayeri. Two tests were conducted to determine the effect of a single pollution event (test 1) compared with a recurring repeated pulse pollution event (test 2). Test 1 required no water changes, while test 2 required renewal of the test treatments on a 4-day basis. Three endpoints were used, un-hatched blastula (48 h to 48.5 h) to represent the embryonic phase, gastrula (10 d) and 4-armed pluteus (16 d to 18 d). At the termination of each endpoint, 1 ml of 10% buffered formalin was added to each relevant vial and recapped. At the conclusion of the experiments, preserved embryos were observed under a dissecting microscope to determine the number of normal, abnormal and unfertilised embryos relative to controls. Samples for analysis of total petroleum hydrocarbon content were taken throughout the 2 experiments to determine the actual concentrations to which embryos and larvae were exposed. The measured concentrations were integrated following the methods of Payne et al. (2014) to obtain a profile of hydrocarbon content over each test period. Two spreadsheets are included in this metadata record detailing survival data and results of hydrocarbon analysis. The survival data file includes test condition details on the first tab, with data for tests 1 and 2 on the second and third tabs. Test treatment and concentration are listed on the left of each data block and count categories are defined in the top left panel. Development stage, date preserved and age of organism is defined for each data block, representing the three endpoints included in the experiments: unhatched blastula, gastrula and 4-armed pluteus. The hydrocarbon analysis, TPH (total petroleum hydrocarbon) file details chemical analysis results produced by K. Kotzakoulais at Macquarie University as part of project 4142. Row terminology explanations are as follows: TPH metadata Test name- indicates the tested species Exp number-indicates whether the data belongs to test 1 or test 2 Water change- details the identification of the sample in relation to the 4-day water change regime. Start samples represent the beginning of the experiment. Pre samples are taken at the end of the corresponding 4-day period, before the water is changed. 'Post' samples are taken of newly made test solutions. The chronological order of sampling is therefore: Start, pre4d, post4d, pre8d, post 8d etc. Only 'pre' samples were taken for test 1, as there were no water changes. less than C9 - greater than C28- Hydrocarbon content of samples was broken down into four compound size classes detailed for each analysis. Contamination- contamination was detected in samples, the source of contamination remains unclear, however it was established that contamination occurred during the sampling process and therefore did not come into contact with organisms. Contamination was therefore excluded from calculations. The hydrocarbon content of 0.1% dilutions was unable to be reliably analysed due to accuracy of the equipment and interfering contamination. Control data indicates spot checks to confirm the presence or absence of fuel. Very small amounts of hydrocarbons were detected as lighter fuel components evaporated and dissolved into control water within the cabinet. These very small amounts are negligible. Abnormality metadata Tab 1 details test conditions Tab 2 'Test 1' includes the data for test 1 Tab 2 'Test 2' includes the data for test 2. All observational categories are defined within the spreadsheet.

Untreated, macerated wastewater effluent has been discharged to the sea at Davis Station since 2005, when the old wastewater treatment infrastructure was removed. This environmental assessment was instigated to guide the choice of the most suitable wastewater treatment facility at Davis. The assessment will support decisions that enable Australia to meet the standards set for the discharge of wastewaters in Antarctica in national legislation (Waste Management Regulations of the Antarctic Treaty Environmental Protection Act - ATEP) and to meet international commitments (the Madrid Protocol) and to meet Australia's aspirations to be a leader in Antarctic environmental protection. The overall objective was to provide environmental information in support of an operational infrastructure project to upgrade wastewater treatment at Davis. This information is required to ensure that the upgrade satisfies national legislation (ATEP/Waste Management Regulations), international commitments (the Madrid Protocol) and maintain the AAD's status as an international leader in environmental management. The specific objectives were to: 1. Wastewater properties: Determine the properties of discharged wastewater (contaminant levels, toxicity, microbiological hazards) as the basis for recommendations on the required level of treatment and provide further consideration of what might constitute adequate dilution and dispersal for discharge to the nearshore marine environment 2. Dispersal and dilution characteristics of marine environment: Assess the dispersing characteristics of the immediate nearshore marine environment in the vicinity of Davis Station to determine whether conditions at the existing site of effluent discharge are adequate to meet the ATEP requirement of initial dilution and rapid dispersal. 3. Environmental impacts: Describe the nature and extent of impacts to the marine environment associated with present wastewater discharge practices at Davis and determine whether wastewater discharge practices have adversely affected the local environment. 4. Evaluate treatment options: Evaluate the different levels of treatment required to mitigate and/or prevent various environmental impacts and reduce environmental risks.

The data set includes information relevant for the study and description of sea-ice bacteria contains the following dataset subgroups and is organised by REFERENCE number. 1) Isolation data: strain designations (e.g. culture collection names are indicated for type cultures); media used for isolation and routine cultivation; temperature used for incubation; any special conditions (e.g. enrichment conditions) used for isolation; isolation site and type (e.g. sea-ice); availability of the indicated strain from the chief investigator (J. Bowman) 2) Phenotypic data: Includes morphological, physiological and biochemical tests performed. Details on how these were performed are indicated in the relevant reference. 3) Growth/temperature data: data for temperature related growth curves are given where available. Methods are indicated in the associated reference. 4) Fatty acid/chemotaxonomy data: fatty acid and other related data are given where available. Methods are indicated in the associated reference. 5) Genotypic data: data for DNA-guanosine/cytosine-content and genomic DNA:DNA hybridization are shown where available. Methods are indicated in the associated reference. 6) Phylogenetic data: data for sequences are cross-referenced to the GenBank database. In some cases, aligned sequence datasets are available in FASTA format and can be viewed in the programs BIOEDIT (www.mbio.ncsu.edu/BioEdit/bioedit.html) or CLUSTAL W (www.ebi.ac.uk/clustalw). 7) Other related published references which are useful or relevant to the dataset e.g. related sequences published subsequent to the ASAC study