Metadata record for data from ASAC Project 867 See the link below for public details on this project. Dataset Sea-ice bacteria data are associated with ASAC_1012 and included there Data for bacteria from ornithogenic soil samples collected from the Vestfold Hills Region is included (associated with ref 9899): 1) Isolate designations, availability, media used and growth conditions. 2) Phenotypic data - morphology, nutritional and biochemical traits 3) Chemical data - fatty acids, wax esters 4) Genotypic data - DNA base composition, DNA:DNA hybridisation analysis 5) Phylogenetic data - 16S rRNA gene sequences The download file contains: Sample data obtained. Includes sea-ice sampling sites, location, information on ice cores including presence or absence of algal assemblage band communities and whether under-ice seawater was collected or not. Samples were melted and/or melted then filtered (0.2 micron size) for cultivation and DNA-related analyses carried out primarily in AAS project 1012.

The data set includes information relevant for the study and description of sea-ice bacteria contains the following dataset subgroups and is organised by REFERENCE number. 1) Isolation data: strain designations (e.g. culture collection names are indicated for type cultures); media used for isolation and routine cultivation; temperature used for incubation; any special conditions (e.g. enrichment conditions) used for isolation; isolation site and type (e.g. sea-ice); availability of the indicated strain from the chief investigator (J. Bowman) 2) Phenotypic data: Includes morphological, physiological and biochemical tests performed. Details on how these were performed are indicated in the relevant reference. 3) Growth/temperature data: data for temperature related growth curves are given where available. Methods are indicated in the associated reference. 4) Fatty acid/chemotaxonomy data: fatty acid and other related data are given where available. Methods are indicated in the associated reference. 5) Genotypic data: data for DNA-guanosine/cytosine-content and genomic DNA:DNA hybridization are shown where available. Methods are indicated in the associated reference. 6) Phylogenetic data: data for sequences are cross-referenced to the GenBank database. In some cases, aligned sequence datasets are available in FASTA format and can be viewed in the programs BIOEDIT (www.mbio.ncsu.edu/BioEdit/bioedit.html) or CLUSTAL W (www.ebi.ac.uk/clustalw). 7) Other related published references which are useful or relevant to the dataset e.g. related sequences published subsequent to the ASAC study