INDICATOR DEFINITION Count of all adult females, fully weaned pups and dead pups hauled out on, or close to, the day of maximum cow numbers, set for October 15. TYPE OF INDICATOR There are three types of indicators used in this report: 1.Describes the CONDITION of important elements of a system; 2.Show the extent of the major PRESSURES exerted on a system; 3.Determine RESPONSES to either condition or changes in the condition of a system. This indicator is one of: CONDITION RATIONALE FOR INDICATOR SELECTION Elephant seals from Macquarie Island are long distance foragers who can utilise the Southern Ocean both west as far as Heard Island and east as far as the Ross Sea. Thus their populations reflect foraging conditions across a vast area. The slow decline in their numbers (-2.3% annually from 1988-1993) suggests that their ocean foraging has been more difficult in recent decades. Furthermore, interactions with humans are negligible due to the absence of significant overlap in their diet with commercial fisheries. This suggests that changes in 'natural' ocean conditions may have altered aspects of prey availability. It is clear that seal numbers are changing in response to ocean conditions but at the moment these conditions cannot be specified. DESIGN AND STRATEGY FOR INDICATOR MONITORING PROGRAM Spatial Scale: Five beaches on Macquarie Island (lat54 degrees 37' 59.9' S, long 158 degrees 52' 59.9' E): North Head to Aurora Point; Aurora Point to Caroline Cove; Garden Cove to Sandy Bay; Sandy Bay to Waterfall Bay; Waterfall Bay to Hurd Point. Frequency: Annual census on 15th October Measurement Technique: Monitoring the Southern Elephant Seal population on Macquarie island requires a one day whole island adult female census on October 15 and a daily count of cow numbers, fully weaned pups and dead pups on the west and east isthmus beaches throughout October. Daily cow counts during October, along the isthmus beaches close to the Station, provide data to identify exactly the day of maximum numbers. The isthmus counts are recorded under the long-established (since 1950) harem names. Daily counts allow adjustment to the census totals if the day of maximum numbers of cows ashore happens to fall on either side of October 15. Personnel need to be dispersed around the island by October 15 so that all beaches are counted for seals on that day. This has been achieved successfully for the last 15 years. On the day of maximum haul out (around 15th October) the only Elephant seals present are cows, their young pups and adult males. The three classes can be readily distinguished and counted accurately. Lactating pups are not counted, their numbers are provided by the cow count on a 1:1 proportion. The combined count of cows, fully weaned pups and dead pups provides an index of pup production. The count of any group is made until there is agreement between counts to better than +/- 5%. Thus there is always a double count as a minimum; the number of counts can reach double figures when a large group is enumerated. The largest single group on Macquarie Island is that at West Razorback with greater than 1,000 cows; Multiple counts are always required there. RESEARCH ISSUES Much research has been done already to acquire demographic data so that population models can be produced. Thus there will be predicted population sizes for elephant seals on Macquarie Island in 2002 onwards and the annual censuses will allow these predictions to be tested against the actual numbers. The censuses are also a check on the population status of this endangered species. LINKS TO OTHER INDICATORS

Elephant seals use a suite of physiological and behavioural mechanisms to maximise the time they can be submerged. Of these hypo-metabolism is one of the most important, so this study quantified maximum O2 consumptions relative to dove depth and swim speed. From the abstract of the referenced paper: The ability of air-breathing marine predators to forage successfully depends on their ability to remain submerged. This is in turn related to their total O2 stores and the rate at which these stores are used up while submerged. Body size was positively related to dive duration in a sample of 34 adult female southern elephant seals from Macquarie Island. However, there was no relationship between body size and dive depth. This indicates that smaller seals, with smaller total O2 stores, make shorter dives than larger individuals but operate at similar depths, resulting in less time being spent at depth. Nine adult female elephant seals were also equipped with velocity time depth recorders. In eight of these seals, a plot of swimming speed against dive duration revealed a cloud of points with a clear upper boundary. This boundary could be described using regression analysis and gave a significant negative relationship in most cases. These results indicate that metabolic rate varies with activity levels, as indicated by swimming speed, and that there are quantifiable limits to the distance that a seal can travel on a dive of a given swimming speed. However, the seals rarely dive to these physiological limits, and the majority of their dives are well within their aerobic capacity. Elephant seals therefore appear to dive in a way that ensures that they have a reserve of O2 available. Data were collected on Time Depth Recorders (TDRs), and stored in hexadecimal format. Hexadecimal files can be read using 'Instrument Helper', a free download from Wildlife Computers (see the url given below). Data for this project is the same data that was collected for ASAC projects 857 and 589 (ASAC_857 and ASAC_589).

This database is a compendium of histories of known age seals (Weddell and Southern elephant) from observations across the Southern Ocean but focussed on Macquarie Island, Marion Island, Heard Island, Mawson and the Vestfold Hills. At Macquarie Island 1000 seals were weighed per annum between 1993-2003 at birth and individually marked with two plastic flipper tags in the inter-digital webbing of their hind flippers. These tagged seals were weighed again at weaning, when length, girth, fat depth, and flipper measurements were made. Three weeks after weaning 2000 seals were permanently and individually marked by hot-iron branding. Recaptures and re-weighings of these known aged individuals were used to calculate growth and age-specific survival of the seals. Similar data were collected from elephant seals between 1950 and 1965 when seals were individually marked by hot-iron branding. Mark-recapture data from these cohorts were used to assess the demography of the declining population. Length and mass data were also collected for these cohorts and were used, for the first time, to assess the growth of individual seals without killing them. At Marion Island all the elephant seals have been individually marked with two plastic flipper tags in their rear flippers. Recaptures of these seals were used to compare survival at Marion and Macquarie Islands. At Heard Island, seals were branded between 1949-1953. Seal length was measured in feet and inches. Recaptures of seals were made up until 1955, and growth and age-specific survival was calculated. Survival data from Heard Island were compared with concurrent data from Macquarie Island. The database was held by the Australian Antarctic Data Centre, but was taken offline due to maintenance problems. A snapshot of the database was taken in June 2018 and stored in an access database. This work was completed as part of ASAC project 90.

This database is a compendium of histories of known age seals (Southern elephant) from observations across the Southern Ocean but focussed on Macquarie Island, Marion Island, Heard Island, Mawson and the Vestfold Hills. At Macquarie Island 1000 seals were weighed per annum between 1993-2003 at birth and individually marked with two plastic flipper tags in the inter-digital webbing of their hind flippers. These tagged seals were weighed again at weaning, when length, girth, fat depth, and flipper measurements were made. Three weeks after weaning 2000 seals were permanently and individually marked by hot-iron branding. Recaptures and re-weighings of these known aged individuals were used to calculate growth and age-specific survival of the seals. Similar data were collected from elephant seals between 1950 and 1965 when seals were individually marked by hot-iron branding. Mark-recapture data from these cohorts were used to assess the demography of the declining population. Length and mass data were also collected for these cohorts and were used, for the first time, to assess the growth of individual seals without killing them. At Marion Island all the elephant seals have been individually marked with two plastic flipper tags in their rear flippers. Recaptures of these seals were used to compare survival at Marion and Macquarie Islands. At Heard Island, seals were branded between 1949-1953. Seal length was measured in feet and inches. Recaptures of seals were made up until 1955, and growth and age-specific survival was calculated. Survival data from Heard Island were compared with concurrent data from Macquarie Island. The database was held by the Australian Antarctic Data Centre, but was taken offline due to maintenance problems. A snapshot of the database was taken in January 2015 and stored in an access database and several csv files. This work was completed as part of ASAC project 90.

The objectives for this project were: The project aims to quantify the patterns of dispersal and survival of newly weaned southern elephant seal pups to provide information on position at sea and foraging behaviour of the pups once they leave Macquarie Island, and to examine how this is related to position at sea and foraging behaviour in the second year. This information will be used to test the hypothesis that first year survival is a consequence of the young animals exploiting different foraging grounds to adults, and that fishing activity on the Campbell Plateau may be a contributing factor. In addition, stable isotope analysis and fatty acid signature analysis will be used to examine differences in foraging behaviour from animals while they are at sea. The raw data from this project is added to the long term database described by the metadata records 'Macquarie Island Elephant Seal Populations 1950-1965', and 'Macquarie Island Elephant Seal Populations 1985 Onwards'. This database has been taken offline, however. A snapshot of the database was taken in January, 1995, and is linked at the provided URL. For access, contact the Australian Antarctic Data Centre. A number of papers have been produced from this project. Some of these papers are included in the reference section below. The data collected for the database is as follows: Seal Number Status (new or resight) Date Location Age Class Status (cow, beachmaster, pregnant cow, dead etc) Sex Weight Length Size Back Fat Flipper Body Water Time Depth Recorder

Twenty three juvenile (8-14 months of age) southern elephant seals (Mirounga leonina L.) from Macquarie Island were tracked during 1993 and 1995. Migratory tracks and ocean areas with concentrated activity, presumed to be foraging grounds, were established from location data gathered by attached geolocation time depth recorders. The seals ranged widely (811-3258 km) and foraging activity centred on oceanographic frontal systems, especially the Antarctic Polar Front and bathymetric features such as the Campbell Plateau region. The seals spent 58.6% of their sea time within managed fishery areas while the remainder was spent on the high seas, an area of unregulated fishing. The Commission for the Conservation of Antarctic Marine Living Resources (CCAMLR) areas 58.4.1, 88.2 and especially 88.1 were important and distant foraging areas for these juvenile elephant seals. From fisheries records, diet and the foraging ecology studies of the seals there appears to be little, if any, overlap or conflict between the seals and commercial fishing operations within the regulated commercial areas. However, attention is drawn to the possibility of future interactions if Southern Ocean fisheries expand or new ones commence. Furthermore... The dive duration of 16 underyearling (6-12 months old) southern elephant seals Mirounga leonina during their second trip to sea was investigated using geolocating time depth recorders. Underyearling seals had a lesser diving ability, with respect to duration and depth, than adult southern elephant seals. Individual underyearlings dived for average durations of up to 20.3 minutes and depths up to 416m compared to durations and depths of 36.9 minutes and 589m, respectively for adults. Dive duration was positively related to their body mass at departure, indicating that smaller seals were limited to shorter dive durations, perhaps as a result of their lesser aerobic capacity. All seals often exceeded their theoretical aerobic dive limit (average of 22.1 plus/minus 18.1%). The number of dives exceeding the theoretical aerobic dive limit was not related to mass, suggesting that factors other than mass, such as foraging location or prey availability, may have been responsible for the differences in diving effort. Foraging ability, indicated by the ability of the seals to follow vertically moving prey, was positively related to seal mass, indicating that small mass restricted foraging ability. The shorter dive durations of the smaller seals inferred that they had shallower dive depths in which to search for prey, thus restricting foraging ability. Although foraging ability was restricted by size, foraging success was found to be inversely related to mass, the smaller seals gaining a higher proportion of blubber than larger seals during their foraging trips. Thus, despite smaller seals being restricted to shallower depths and shorter durations, their foraging success was not affected. The fields in this dataset are: Area Perimeter ID Latitude Longitude Time Percent CCAMLR EEZ Season Seal Sex Age (months) Days at Sea Range (km) Bearing (degrees) Sea Surface Temperatures (degrees C) Foraging Areas Departure Mass (kg) At sea mass gain (kg) Rate of mass gain (kg) Survival estimates Length (m) Girth (m) Dives Divers per hour Total Time Diving % trip diving Dive Duration Surface Time Theoretical Aerobic Dive Limit Drift

A variety of different chemical restraints, or anaesthetics, were trialed on Southern Elephant Seals at Heard Island and Macquarie Island. The trials were performed on female and juvenile seals, in their pre-moult stages. Further information can be found in the papers listed in the reference section below. The chemicals used in this study include: doxapram ketamine xylazine midazolam pethidine thiopentone cyclohexamine based compounds diazepam tiletamine zolazepam 4-aminopyridine Sarmazenil Yohimbine The fields in this dataset are: Anaesthetic Antagonist Dose Mass Time Heart Rate Respiratory Rate Side Effects pH PvO2 PvCO2 HCO3 Total CO2

APIS data were collected between 1994 and 1999. This dataset also includes some historical data collected between 1985 and 1987. Both aerial and ship-board surveys were conducted. Studies on the behaviour of Pack-ice or Crabeater Seal (Lobodon carcinophagus) in the Southern Ocean and in the Australian Sector of Antarctica were also conducted as part of this study. Satellite tracking was used to determine their movement, durations on land and at sea, dive depths and dive duration etc. The four species of Antarctic pack ice seals (crabeater, leopard, Weddell, and Ross seals) are thought to comprise up to 50% or more of the world's total biomass of seals. As long-lived, top level predators in Southern Ocean ecosystems, pack ice seals are scientifically interesting because they can assist in monitoring shifts in ecosystem structure and function, especially changes that occur in sensitive polar areas in response to global climate changes. The APIS Program focuses on the ecological importance of pack ice seals and their interactions with physical and biotic features of their environment. This program is a collaborative, multi-disciplinary research initiative whose planning and implementation has involved scientists from more than a dozen countries. It is being developed and coordinated by the Group of Specialists on Seals of the Scientific Committee on Antarctic Research (SCAR), and represents an important contribution to SCAR's Antarctic Global Change Program. Australian researchers have undertaken an ambitious science program studying the distribution and abundance of pack ice seals in support of the APIS Program. An excellent overview of this work is provided at the Australian Antarctic Division's web site. The following paragraphs provide a brief progress report of some of that work through 1998. ------------------------------------------------------------------------------- Four years of developmental work have now been completed in preparation for the Australian contribution to the circumpolar survey that will take place in December 1998. Until recently the main effort has been directed towards designing and building a system for automatic data logging of line transect data by double observers. Two systems identical in concept have been designed for aerial survey and shipboard survey. The systems consist of a number of sighting guns and keypads linked to a central computer. The sightings guns are used to measure the exact time and angle of declination from the horizon of seals passing abeam of the survey platform. Also logged regularly (10 second intervals) are GPS position and altitude (aerial survey only). The aerial survey system also has an audio backup. The aerial survey system has been trialled over three seasons and the shipboard system over one season. Preliminary analysis of aerial data indicates that the essential assumption of the line transect method is badly violated, reinforcing the need for double observers. Assumption violation is likely to be less in shipboard survey, but assessment of the assumption of perfect sightability on the line is still important. User manuals have been written for both the aerial and shipboard systems. An aerial survey system is being constructed for use by BAS in the coming season. A backup manual system for aerial and shipboard survey has also been developed in the event of the automatic system failing. The aerial backup system uses the perspex sighting frame developed by the US. A database has been designed for storage and analysis of aerial and shipboard data. Importing of data is fast and easy, allowing post-survey analysis and review immediately after each day's survey effort. Aides for training observers have been developed. A video on species identification has been produced. A Powerpoint slide show has been designed to simulate aerial survey conditions and use of the automatic data logging system. Currently effort has been directed toward developing an optimal survey design. While a general survey plan is necessary, it must be flexible to deal with unpredictable ice and weather conditions. It is planned to use both the ship and two Sikorsky 76 helicopters as survey platforms. The ship will be used to survey into and out from stations, and inwards from the ice edge for approximately 60 miles. The helicopters will be used to survey southwards from the ship for distances up to 140 miles in favourable weather. Helicopters will fly in tandem, with transects 10 miles apart. Studies of crabeater seal haul-out behaviour have been conducted over the past four seasons. Twenty SLTDRs have been deployed in the breeding season (September-October). The length of deployments varies from a few days to 3 months. No transmissions have been received after mid-January, probably due to loss of instruments during the moult. Most instruments have transmitted data through the survey period of November-December. Haul-out behaviour is consistent between animals and years. However, five more instruments will be deployed in the survey season to ensure there is haul-out data concurrent with the survey effort. Some observations of penguins and whales were also made. The accompanying dataset includes three Microsoft Access databases (stored in both Access 97 and Access 2002 formats), as well as two Microsoft Word documents, which provide additional information about these data. The fields in this dataset are: Date Time Time since previous sighting Side (of aircraft/ship) Seen by (observer) Latitude Longitude Number of adults Number of pups Species (LPD - Leopard Seal, WED - Weddell Seal, SES - Southern Elephant Seal, CBE - Crabeater Seal, UNS - Unknown Seal, ADE - Adelie Penguin, ROS - Ross Seal, EMP - Emperor Penguin, MKE - Minke Whale, ORC - Orca Whale, UNP - Unknown Penguin, UNW - Unknown Whale) SpCert - How certain the observer was of correct identification - a tick indicates certainty Distance from Observer (metres) Movement Categories - N: no data, S: stationary, MB: moved body, MBP: moved body and position, movement distance: -99 no data, negative values moved towards flight line, positive distance moved away from flight line Distance dart gun fired from animal (in metres) Approach method (S = ship, H = helicopter, Z = unknown) Approach distance (metres) Group (S = single, P = pair, F = family (male, female and pup)) Sex Guessed Weight (kg) Drugs used Maximum Sedation Level (CS = Colin Southwell, MT = Mark Tahmidjis) Time to maximum sedation level Time to return to normal Heart rate (maximum, minimum) Respiration rate (maximum, minimum, resting) Arousal Level (1 = calm, 2 = slight, 3 = strong) Arousal Level Cat1 (1 = calm, 2 = 2+3 from above) Apnoea (maximum length of apnoea in minutes) Comments Time at depth - reading taken every 10 seconds, and whichever depth incremented upwards by 1. Time period (NT - 21:00-03:00, MN - 03:00-09:00, MD - 09:00-15:00, AF - 15:00-21:00) Seal Age - (A = Adult, SA = sub-Adult) WCId - Wildlife Computers Identification Number for SLTDR Length, width, girth (body, head, flippers) (cm) Blood, blubber, skin, hair, tooth, scat, nasal swab - sample taken, yes or no. In general, Y = Yes, N = No, ND = No Data This work was also completed as part of ASAC projects 775 and 2263.