Metadata record for data from AAS (ASAC) project 3051. Public Environmental change is by far one of the major crises facing our planet in recent times. This project will contribute specifically to understanding the effects of climate change and other human-induced impacts on marine species in Antarctica. Through studying key ecological and biological processes in marine benthic invertebrates we will better understand the spatial scale of populations, the nature of the processes that maintain those populations, how environmental change will affect those processes, and the levels of genetic diversity and resilience in Antarctic marine communities. Taken together this information will enable better, more informed management of Antarctic marine ecosystems. Project objectives: The project objectives, as stated in the project application round 2008/09, appear below: This project will combine experimental tests of demographic change with genetic tests of population isolation and diversity to enable predictions of the resilience of Antarctic marine invertebrates to current and predicted environmental change. The specific objectives of the project are; 1. Effects of change. Understand the effects of environmental change on reproduction (fecundity, reproductive success) and the early life history (larval behaviour, survivorship, and recruitment) of key Antarctic marine benthic invertebrates. 2. Isolation. Determine the degree of isolation/connectivity among populations as well as the levels of genetic diversity of key Antarctic marine benthic invertebrates. 3. Resilience. Assess the resilience (ability to cope with or adapt) of Antarctic marine benthic invertebrates to environmental change. 4. Practical Outcomes. Develop improved predictive capacity to contribute towards the development of management strategies for the conservation of Antarctic marine benthic invertebrates. Taken from the 2008-2009 Progress Report: Progress against objectives: This project commenced in 2008/9. Objective 1 - Effects of change - Collected live echinoderms (Abatus spp, Sterechinus numeyeri, Diploasterias) from around Casey Station and transported these on the A319 back to Kingston. A preliminary fertilisation trial has been run using Sterechinus individuals, and the remaining individuals are now being maintained in aquaria for future reproductive studies. Objective 2 - Isolation - Tissue samples from over 200 Sterechinus numeyeri were collected from 5 sites around Casey Station. These will form the foundation for genetic connectivity studies, and will complement exisiting Abatus samples from the same location. Laboratory processing of these samples has commenced, and development of microsatellite markers for both species is underway. Objectives 3 and 4 represent late-stage components of the project, so no progress can be reported on these at this stage. Taken from the 2009-2010 Progress Report: Progress against the objectives: Objective 1 - Effects of change - Collected live urchins (Abatus spp and Sterechinus numeyeri) from around Davis Station. Ran a series of spawning trials, although these were largely unsuccessful, with most individuals having spawned prior to the beginning of the season. We ran one successful fertilisation trial with S. neumeyeri to look at the effects of water temperature and salinity on fertilisation success. Preliminary analysis of the data indicates these environmental parameters do have an effect on fertilisation. Objective 2 - Isolation - Tissue samples from over 350 Sterechinus numeyeri were collected from 12 sites around Davis Station. These will be used for genetic connectivity studies, and will complement samples collected from Casey in the previous season. Larval Sterechinus were also collected from the water column and preserved for genetic analysis along with adult and juvenile Abatus ingens. Microsatellite markers (11 polymorphic loci) have now been developed for Sterechinus, and microsatellite deveopment is partially completed for Abatus ingens; the library has been created but optimisation of loci still needs to be done. We have completed DNA sequencing for Sterechinus and Abatus from Casey Station for 1 gene region (16S) and are optimising an additional 2 regions. This will be used to compare populations from Davis and Casey to understand large-scale connectivity. Objectives 3 and 4 represent late-stage components of the project. As this is only the second year of a 5-year programme, no progress can be reported on these at this stage.

This dataset comprises the actual video footage and audio recordings made during a number of experiments made as part of ASAC project 1148 (ASAC_1148). The primary objective was to measure the responses of Antarctic wildlife to various human disturbance stimuli. An excel spreadsheet of a catalogue of the video and audio tapes is available for download from the url given below. The video and audio tapes themselves are stored at the Australian Antarctic Division. For descriptions of (and access to) processed data see the metadata records with the following titles: Measuring the effects of human activity on Weddell Seals (Leptonychotes weddellii) Effects of helicopter operations on emperor penguin chicks Effects of helicopters on Southern Antarctic Fulmars Effects of helicopters on Antarctic wildlife Effects of human activity on Gentoo penguins on Macquarie Island Effects of human activity on King penguins on Macquarie Island Effects of human activity on Royal penguins on Macquarie Island Behavioural responses of Weddell seals to human activity. A copy of the full dataset of video and audio files, as well as another Excel spreadsheet catalogue is available for download from the provided URL. These data were digitised in 2021, and the excel spreadsheet created from the available files.

From the abstract of the referenced paper: One hundred and sixty four plastic particles (mean length 4.1 mm) recovered from the scats of fur seals (Arctocephalus spp.) on Macquarie Island were examined. Electron micrographs of 41 of the plastic particles showed that none could be identified as plastic pellet feedstock from their shapes. Commonly, such pellets are cylindrical and spherical. Instead, all the 164 plastic particles from the seal scats were angular particles of 7 colors (feedstock particles are normally opaque or white) and could be classified into 2 categories: i) fragmented along crystal lines and likely to be the result of UV breakdown; and ii) worn by abrasion (where striations were clearly visible) into irregular shapes with rounded corners. White, brown, green, yellow and blue were the most common colors. In composition, they came from 5 polymer groups; polyethylene 93%, polypropylene 4%, poly(1-Cl-1-butenylene) polychloroprene 2%, melamine-urea (phenol) (formaldehyde) resin 0.5%, and cellulose (rope fiber) 0.5%. The larger groups are buoyant with a specific gravity less than that of seawater. These small plastic particles are formed from the breakdown of larger particles (fragments). Their origin seems to be from the breakdown of user plastics washed ashore and ground down on cobbled beaches. Certainly most particles (70%) had attained their final form by active abrasion. It is hypothesized that the plastic particles were washed out to sea and then selected by size and consumed by individuals of a pelagic fish species, Electrona subaspera, who in turn were consumed by the fur seals. Thus, the particles were accumulated both by the fish and the seals in the usual process of their feeding. The download file contains a pdf of the paper listed in the reference section below, as well as 48 scanning electron micrograph images of plastics recovered from fur seal scats.

From the abstract of the referenced paper: 1. Incidental mortality in fisheries is causing declines in many albatross populations around the world. To assess potential interactions with regional fisheries satellite tags were used to track black-browed albatrosses (Thalassarche melanophrys) and light-mantled sooty albatrosses (Phoebetria palpebrata) breeding on Heard Island during the chick-rearing periods of 2003/2004. This was the first time individuals from either population had been tracked. 2. Black-browed albatrosses foraged largely within the Heard and McDonald Islands Economic Exclusion Zone (EEZ) north-east of the island, although 20% of foraging trips were to areas north of the EEZ into Commission for the Conservation of Antarctic Marine Living Resources (CCAMLR) areas 58.5.1 and 58.5.2 and into the Iles Kerguelen EEZ. 3. In contrast, the light-mantled sooty albatrosses foraged well south of Heard Island along the southern boundary of the Antarctic Circumpolar Current. Both species appear to face minimal risk of incidental mortality during the chick-rearing period in the regulated, legal fisheries, but are threatened by illegal, unreported and unregulated (IUU) fishing vessels operating in the southern Indian Ocean. The data have been loaded into the ARGOS database held by the Australian Antarctic Data Centre. An excel spreadsheet detailing PTT number (position tracking terminal), date of attachment, date of retrieval and species is also provided as an aide to searching the ARGOS database. Two articles are also associated with this record, a refereed journal article, plus an article in the Australian Antarctic Division's Antarctic Magazine. The fields in this dataset are: Species PTT number Date of attachment Date of retrieval Latitude Longitude Time

This study aimed to quantify the effects of helicopter operations on Antarctic wildlife, with an emphasis on determining minimum safe over-flight altitudes and landing distances for a range of species. An experimental approach was adopted whereby wildlife were exposed to helicopters either over-flying or landing at specific altitudes or distances while the behaviour, and in some cases physiology, of individual animals were recorded. Two types of helicopters were used in the study: a Sikorsky S-76 (twin engine) and a Squirrel AS350 (single engine). This metadata record relates to the responses of Adelie Penguins (Pygoscelis adeliae) over a number of phases of their breeding cycle. The fields in this dataset are: Time Action Date

Creching emperor penguin (Aptenodytes forsteri) chickswere exposed to two overflights by an S-76 twin engine helicopter at 1000 m: a current operational guideline for helicopter activity in Antarctica. The flights were conducted on the same day but under different wind conditions: a morning flight with a 10 kt (18 km.hr-1) katabatic blowing perpendicular to the direction of helicopter travel and an afternoon flight with virtually no wind. Background noise levels recorded in the morning, before the helicopter flight, were significantly higher than in the afternoon, but these differences were not detectable when the helicopter was overhead. There were also no significant differences in the way chicks responded to helicopters between the morning and afternoon flight. All chicks became more vigilant when the helicopter approached and 69% either walked or ran, generally moving less than 10 m toward other chicks (i.e. not scattering). Most chicks (83%) displayed flipper-flapping, probably indicating nervous apprehension. This behaviour was seldom displayed in the absence of disturbance. Although all effects were relatively transitory, results support the introduction of more conservative guidelines for helicopter operations around breeding localities of this species. The fields in this dataset are: Time Action Date Lying Standing Walking Preening Flapping

Sediment cores (5cm diameter x 10cm deep), collected as part of the long-term monitoring of the Thala Valley waste disposal site clean-up (Casey station), were sectioned and a portion of each core analysed for a range of heavy metals. Metals were extracted from the sediment via a 4 hour 1M HCl acid extraction. Concentrations were gained from ICP-MS analysis of the resulting extracts (ICP-MS conducted at the School of Chemistry, University of Tasmania). Cores were collected from various control and potentially impacted sites in the Windmill Islands around Casey station. This work was conducted as part of ASAC 2201 (ASAC_2201).

This project empirically measures the effects of human activity on the behaviour and reproductive success of Gentoo penguins on Macquarie Island. This was achieved by 1) collecting behavioural responses of individual penguins exposed to pedestrian approaches across the breeding stages of guard, creche and moult, and 2) collecting reproductive success data (chicks raised to creche age per nesting pair) for gentoo penguins colonies in areas of high and low human activity. Information produced includes minimum approach guidelines. As of April 2003 all data are stored on Hi-8 digital tape, due to be transformed during 2003 - 2004 into a timecoded tab-delimited text format for analysis using the Observer (Noldus Information Technology 2002). This work was carried out as part of ASAC project 1148 (ASAC_1148). The fields in this dataset are: Sample Date Breeding Phase Stimulus Type Colony Focal birds tape number Wide angle tape number Location within colony Weather Time Windspeed Temperature Precipitation Cloud Pre-approach control Approach Post-approach control Maximum approach distance

This project empirically measures the effects of human activity on the behaviour of King penguins on Macquarie Island, under ASAC project 1148. This was achieved by collecting behavioural responses of individual penguins exposed to pedestrian approaches across the breeding stages of incubation and guard. Information produced includes minimum approach guidelines. As of April 2003 all data are stored on Hi-8 digital tape, due to be transformed during 2003 - 2004 into a timecoded tab-delimited text format for analysis using the Observer (Noldus Information Technology 2002). The fields in this dataset are: Sample Date Breeding Phase Approach Colony Focal birds tape number Wide angle tape number Weather Time Windspeed Temperature Precipitation Cloud Pre-approach control Post-approach control Maximum approach distance

This project empirically measures the effects of human activity on the behaviour, heart rate and egg-shell surface temperature of Royal penguins on Macquarie Island, as part of ASAC project 1148. This was achieved by collecting behavioural and physiological responses of individual penguins exposed to pedestrian approaches across the breeding stages of incubation, guard, creche and moult. Both single person and group approaches were also investigated. Information produced includes minimum approach guidelines. As of April 2003 all data are stored on Hi-8 digital tape, due to be transformed during 2003 - 2004 into a timecoded tab-delimited text format for analysis using the Observer (Noldus Information Technology 2002). Some notes about some of the fields in this dataset: Temp file refers to whether or not egg shell surface temperature was also recorded for the sample, with the code below refering to the file name. Neighbour refers to the behavioural control file for each sample (neighbouring nests did not recieve an artificial egg, and provide a behavioural control for responses to human approaches without the scientific treatment). Nest refers to the randomly used nest markers for each sample. Heart rate refers to whether heart rate was concurrently recorded with behaviour on the sample (both stored on Hi-8 tape). Stimulus refers to whether single persons or groups of persons (5 -7, recorded within each sample) were used for the human approaches. Environment refers to whether approaches were conducted from colony sections abuting pebbly beach or from poa tussock environs (tussock approaches less than 50 m of the poa / pebbly beach junction). The code system for nest simply refers to the numbered tag placed at the nest (using three colours, g=green, w=white, b=brown) which were used randomly. The fields in this dataset are: Sample Date Breeding Phase Stimulus Type Environment Colony Nest Tape Heart Rate Temp File Neighbour