Exopolysaccharide (EPS) is complex sugar made by many microbes in the Antarctic marine environment. This project seeks to understand the ecological role of microbial EPS in the Southern Ocean, where it is known to strongly influence primary production. We will investigate the chemical composition and structure of EPS obtained from Antarctic microbes, which will improve our knowledge of its ecological significance and biotechnological potential. Dataset includes the following: 1) Information on Exopolysaccharide-producing bacterial isolates, isolation sites, media used and growth conditions. 2) 16S rRNA gene sequence and fatty acid data of isolates for strain identification. 3) Exopolysaccharide chemistry data including EPS carbohydrate composition, organic acid composition, sulfate content, molecular weight. 4) Physiology of exopolysaccharide synthesis. Effects of temperature and other factors on EPS yield and glucose conversion efficiency. 5) Iron binding properties. The download file includes: 11 files File 1. Bacterial isolate 16S rRNA gene sequences obtained from Southern Ocean seawater or ice samples. The sequences are all deposited on the GenBank nucleotide (NCBI) database. Sequences are in FASTA format. File 2. Seawater and sea-ice sample information including sites samples, sample type. File 3. Data for exopolysaccharide (EPS)-producing bacteria isolated and subsequently selected for further studied. Information indicates special treatments used to obtain strains including plankton towing, filtration method, and enrichment. Identification to species level was determined by 16S rRNA gene sequence analysis. File 4. EPS-producing bacterial isolate fatty acid content determined using GC/MS procedures. File 5. Basic chemical data for EPS from Antarctic isolates including protein, sulfate, and sugar type relative content (determined by chemical procedures), molecular weight in kilodaltons and polydispersity (determined by gel-based molecular seiving). File 6 Monosaccharide unit composition determined by GC/MS of EPS from Antarctic isolates. File 7. Effect of temperature on culture viscosity and growth of EPS-producing bacterium Pseudoalteromonas sp. CAM025 as affected by temperature. File 8. Effect of temperature on EPS and cell yields and EPS synthesis efficiency (as indicated by glucose consumption) of EPS-producing bacterium Pseudoalteromonas sp. CAM025 as affected by temperature. File 9. Efficiency of copper and cadmium metal ion adsorption onto EPS from EPS-producing bacterium Pseudoalteromonas sp. CAM025. File 10. Phenotypic characteristics data for novel EPS-producing Antarctic strain CAM030. Represents type strain of Olleya marilimosa. File 11. Effect of temperature on chemical make up of EPS from EPS-producing bacterium Pseudoalteromonas sp. CAM025.

Antarctic sediments and sea-ice are important regulators in global biogeochemical and atmospheric cycles. These ecosystems contain a diverse range of bacteria whose biogeochemical roles remains largely unknown and which inhabit what are continually low temperature habitats. An integrated molecular and chemical approach will be used to investigate the coupling of microbial biogeochemical processes with community structure and cold adaptation within coastal Antarctic marine sediments and within sea-ice. Overall the project expects to make an important contribution to our understanding of biological processes within low temperature habitats. DATA SET ORGANISATION: The dataset is organised on the basis of publication and is organised on the basis of the following sections: 1. SEDIMENT SAMPLES and ISOLATES Samples collected are described in terms of location, type and where data were obtained chemical features. The designation, source, media used for cultivation and isolation and availability of sediment and other related isolates are provided. Samples included are from the following locations: Clear Lake, Pendant Lake, Scale Lake, Ace Lake, Burton Lake, Ekho Lake, Organic Lake, Deep lake and Taynaya Bay (Burke Basin), Vestfold Hills region; and the Mertz Glacier Polynya region. 2. BIOMASS and ENZYME ACTIVITY DATA Biomass, numbers and extracellular enzyme activity data are provided for Bacteria and Archaea populations from Mertz Glacier Polynya shelf sediments. 3. FATTY ACID and TETRAETHER LIPID DATA Phospholipid and tetraether lipid data are provided for Mertz Glacier Polynya shelf sediments. Whole cell fatty acid data are provided for various bacterial isolates described officially as new genera or species. 4. RNA HYBRIDISATION DATA RNA hybridisation data for Mertz Glacier Polynya sediment samples is provided, including data for oligonucleotide probes specifc for total Bacteria, Archaea, the Desulfosarcina group (class Deltaproteobacteria, sulfate reducing bacterial clade), phylum Planctomycetes, phylum Bacteroidetes (Cytophaga-Flavobacterium-Bacteroides), class Gammaproteobacteria, sulfur-oxidizing and related bacteria (a subset of class Gammaproteobacteria) and Eukaryota. 5. PHYLOGENETIC DATA 16S rRNA gene sequence data are indicated including aligned datasets for three clone libraries derived from the Mertz Glacier Polynya including GenBank accession numbers. Sequence accession numbers are provided for Vestfold Hills lake sediment samples. In addition GenBank numbers are provided for denaturing gradient gel electrophoresis band sequence data from Mertz Glacier Polynya shelf sediment. Other forms of this DGGE data (banding profile analysis) are available in reference Bowman et al. 2003 (AAD ref 10971).

This record relates to the Australian component of the Latitudinal Gradient Project. The LGP is largely a New Zealand, US and Italian venture, but a small contribution has been made by Australian scientists. The Australian component of this work was completed as part of ASAC projects 2361 and 2682 (ASAC_2361, and ASAC_2682). Data from this project were entered into the herbarium access database, which has been linked to this record. The list below contains details of where and when samples were collected, and also the type of sample and the method of sampling. Cape Hallett and vicinity (2000, 2004): Biodiversity assessment of terrestrial plants (mosses, lichens); Invertebrate collections (mites, Collembola); plant ecology and community analysis; photosynthetic physiology of mosses and lichens; molecular genetics of mosses and lichens. Random sampling for biodiversity studies; point quadrats, releves for vegetation analysis, field laboratory experiments for physiological studies. Dry Valleys: Taylor Valley (1989, 1996), Garwood Valley (2001), Granite Harbour (1989; 1994, 1996) - plant ecology; plant physiology; biodiversity; invertebrate collections; molecular genetics of mosses. Random sampling for biodiversity studies; point quadrats, releves for vegetation analysis, field laboratory experiments for physiological studies. Beaufort Island (1996) - plant biodiversity; molecular genetics of mosses. Random sampling for biodiversity studies; point quadrats, releves for vegetation analysis, laboratory studies for molecular genetics. Darwin Glacier (1994): plant biodiversity; molecular genetics of invertebrates and mosses (random sampling for biodiversity; laboratory studies of invertebrate and moss molecular genetics). Project objectives: 1. Investigate the distribution of bryophytes and lichens in continental Antarctica 1a). to test the null hypothesis that species diversity does not change significantly with latitude; 1b). to explore the relationships between species and key environmental attributes including latitude, distance from the coast, temperature, substrate, snow cover, age of ice-free substrate. 2. To continue to participate in the Ross Sea Sector Latitudinal Gradient Project and develop an Australian corollary in the Prince Charles Mountains, involving international collaborators, incorporating the first two objectives of this project. 3. To develop an international collaborative biodiversity and ecophysiological program in the Prince Charles Mountains that will provide a parallel N-S latitude gradient study to mirror the LGP program in the Ross Sea region as part of the present RISCC cooperative program (to be superseded by the EBA (Evolution and Biodiversity of Antarctica) program) to address the above objectives. Taken from the 2008-2009 Progress Report: Progress against objectives: Continuing identification of moss and lichen samples previously collected from Cape Hallett, Granite Harbour and Darwin Glacier region. Lecidea s.l. lichens currently being studied in Austria by PhD student. Field work in Dry Valleys significantly curtailed by adverse weather. Field work planned for Darwin Glacier region and McMurdo Dry Valleys, particularly Taylor Valley and Granite Harbour region was severely curtailed due to adverse weather, helicopter diversions due to a Medical Evacuation, and other logistic constraints. 10 days of field time were lost. Limitations on field travel in Darwin Glacier region restricted the field work to a biologically depauperate region. The Prince Charles Mountains N-S transect, the only continental transect possibility for comparison with the Ross Sea area, unfortunately appears to have been abandoned through lack of logistic support. Taken from the 2009-2010 Progress Report: Identification of samples collected from AAT and Ross Sea Region continued during the year, interrupted significantly by the packing of the collection and transfer of specimens to the Tasmanian Herbarium. Work is now proceeding at the Herbarium with sorting, databasing and incorporation of packets into the Herbarium collection. The merging of the collection provides long-term security of curation and significantly boosts the cryptogam collections (35000 numbers) of the Tasmanian Herbarium.