Exopolysaccharides from Antarctic bacteria

Exopolysaccharide (EPS) is complex sugar made by many microbes in the Antarctic marine environment. This project seeks to understand the ecological role of microbial EPS in the Southern Ocean, where it is known to strongly influence primary production. We will investigate the chemical composition and structure of EPS obtained from Antarctic microbes, which will improve our knowledge of its ecological significance and biotechnological potential.


Dataset includes the following:


1) Information on Exopolysaccharide-producing bacterial isolates, isolation sites, media used and growth conditions.


2) 16S rRNA gene sequence and fatty acid data of isolates for strain identification.


3) Exopolysaccharide chemistry data including EPS carbohydrate composition, organic acid composition, sulfate content, molecular weight.


4) Physiology of exopolysaccharide synthesis. Effects of temperature and other factors on EPS yield and glucose conversion efficiency.


5) Iron binding properties.


The download file includes:

11 files

File 1. Bacterial isolate 16S rRNA gene sequences obtained from Southern Ocean seawater or ice samples. The sequences are all deposited on the GenBank nucleotide (NCBI) database. Sequences are in FASTA format.

File 2. Seawater and sea-ice sample information including sites samples, sample type.

File 3. Data for exopolysaccharide (EPS)-producing bacteria isolated and subsequently selected for further studied. Information indicates special treatments used to obtain strains including plankton towing, filtration method, and enrichment. Identification to species level was determined by 16S rRNA gene sequence analysis.

File 4. EPS-producing bacterial isolate fatty acid content determined using GC/MS procedures.

File 5. Basic chemical data for EPS from Antarctic isolates including protein, sulfate, and sugar type relative content (determined by chemical procedures), molecular weight in kilodaltons and polydispersity (determined by gel-based molecular seiving).

File 6 Monosaccharide unit composition determined by GC/MS of EPS from Antarctic isolates.

File 7. Effect of temperature on culture viscosity and growth of EPS-producing bacterium Pseudoalteromonas sp. CAM025 as affected by temperature.

File 8. Effect of temperature on EPS and cell yields and EPS synthesis efficiency (as indicated by glucose consumption) of EPS-producing bacterium Pseudoalteromonas sp. CAM025 as affected by temperature.

File 9. Efficiency of copper and cadmium metal ion adsorption onto EPS from EPS-producing bacterium Pseudoalteromonas sp. CAM025.

File 10. Phenotypic characteristics data for novel EPS-producing Antarctic strain CAM030. Represents type strain of Olleya marilimosa.

File 11. Effect of temperature on chemical make up of EPS from EPS-producing bacterium Pseudoalteromonas sp. CAM025.

Simple

Identification info

Alternate title
Exopolysaccharides from Antarctic bacteria
Date (Publication)
2011-12-16
Edition
1
Citation identifier
Dataset DOI

Title
Information and documentation - Digital object identifier system
Date (Publication)
2012-04-23
Citation identifier
ISO 26324:2012

Citation identifier
doi:10.4225/15/574BBB8462E3F

Originator

Bowman, J.

Publisher

Australian Antarctic Data Centre

Principal investigator

BOWMAN, JOHN
School of Agricultural Science
University of Tasmania
Private Bag 54
Hobart
Tasmania
7001
Australia

Collaborator

BOWMAN, JOHN
School of Agricultural Science
University of Tasmania
Private Bag 54
Hobart
Tasmania
7001
Australia
Name
CAASM Metadata
Website
https://data.aad.gov.au/metadata/records/ASAC_2348

Status
Completed

Custodian

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Topic category
  • Biota
  • Oceans

Extent

N
S
E
W


Extent

Description
Temporal Coverage

Temporal extent

TimePeriod
2003-07-01 2004-06-30

Extent

Description
Vertical Extent - Depth

Vertical element

Minimum value
0
Maximum value
10
Identifier
urn:x-ogc:def:cs:EPSG:6498
Name
Vertical CS. Axis: depth (D). Orientation: down. UoM: m.
Identifier
urn:x-ogc:def:axis:EPSG:113
Name
depth
Title
Exopolysaccharides from Antarctic bacteria.
Date (Publication)
2004
Citation identifier
96

Author

Mancuso Nichols CA, Bowman JP, Geron S, Raguenes G, Guezennec J.
Name
Journal of Applied Microbiology
Page
1056-1065
NASA/GCMD Earth Science Keywords
  • EARTH SCIENCE > CRYOSPHERE > SEA ICE > PACK ICE
  • EARTH SCIENCE > TERRESTRIAL HYDROSPHERE > WATER QUALITY/WATER CHEMISTRY > CONTAMINANTS > TRACE METALS
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NUTRIENTS
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ORGANIC CARBON
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ORGANIC MATTER
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > TRACE ELEMENTS
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA
  • EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > COASTAL
  • EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > PELAGIC
  • EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > SPECIES COMPETITION
  • EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > EVOLUTIONARY ADAPTATION
  • EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > SURVIVAL RATES
  • EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > BIOGEOCHEMICAL CYCLES
  • EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > FOOD-WEB DYNAMICS
  • EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > NUTRIENT CYCLING
  • EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > DECOMPOSITION
  • EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > BIOMASS DYNAMICS
  • EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > SECONDARY PRODUCTION
  • EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOTOXICOLOGY > BIOAVAILABILITY
Keywords
  • BACTERIA
  • BIODIVERSITY
  • BRINE CHANNELS
  • CARBOHYDRATE CHEMISTRY
  • COLD ADAPTATION
  • EPS
  • EXOPOLYSACCHARIDES
  • FLAVOBACTERIA
  • IRON
  • LIGANDS
  • PSEUDOALTEROMONAS
  • PSYCHROPHILIC BACTERIA
  • SEA ICE
NASA/GCMD Earth Science Keywords
  • NISKIN BOTTLES
  • PLANKTON NETS
  • MICROSCOPES
  • SPECTROPHOTOMETERS
  • FTIR SPECTROMETER > Fourier Transform Infrared Spectrometer
  • Spectrometers
  • GC-MS > Gas Chromatograph/Mass Spectrometry
NASA/GCMD Earth Science Keywords
  • AMD/AU
  • CEOS
  • AMD
NASA/GCMD Earth Science Keywords
  • OCEAN > SOUTHERN OCEAN
  • CONTINENT > ANTARCTICA
  • GEOGRAPHIC REGION > POLAR

Resource constraints

Use limitation
This metadata record is publicly available.

Resource constraints

Access constraints
licence
Other constraints
These data are publicly available for download from the provided URL.

Resource constraints

File type
Portable Network Graphic
Linkage
Creative Commons by Attribution logo

Title
Attribution 4.0 International (CC BY 4.0)
Website
https://creativecommons.org/licenses/by/4.0/legalcode

Legal code for Creative Commons by Attribution 4.0 International license

Use constraints
licence
Other constraints
This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_2348 when using these data.
Language
English
Character encoding
UTF8

Distribution Information

Distributor

Distributor

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Distributor

Fees
Free
Planned available datetime
2011-12-16T00:00:00
Units of distribution
kb
Transfer size
9
Distribution format
  • csv, text

OnLine resource
GET DATA

Download point for the data

OnLine resource
PROJECT HOME PAGE

Public information for ASAC project 2348

OnLine resource
VIEW RELATED INFORMATION

Citation reference for this metadata record and dataset

Maintenance and update frequency
As needed
Maintenance note
2011-12-16 - record updated by Dave Connell after data were supplied by John Bowman. 2016-04-113 - record updated by Dave Connell - basic updates.

Metadata

Metadata identifier
string/ASAC_2348

Language
English
Character encoding
UTF8

Author

BOWMAN, JOHN
School of Agricultural Science
University of Tasmania
Private Bag 54
Hobart
Tasmania
7001
Australia

Sponsor

Australian Antarctic Division

Owner

AADC

Type of resource

Resource scope
Dataset

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
da4058f7-1afb-43b8-8e7e-ae01b8cfc76f

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Date (Last Revision)
2015-11-30T04:52:14

Identifier

Description
metadata.extraction_date

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
8.6

Metadata linkage
http://data.aad.gov.au/metadata/records/ASAC_2348

Point of truth for the metadata record

Date info (Creation)
2003-05-26T00:00:00
Date info (Last Update)
2017-04-26

Metadata standard

Title
ISO 19115-3
Edition
2014
Other citation details
Version 1
Title
DIF to ISO 19115-1 Profile
 
 

Overviews

Spatial extent

N
S
E
W


Keywords


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