Sampling strategy: Samples from trawls or sledges are sieved on the trawl deck then sorted in the wet lab per taxonomic group. Sorting may vary from high taxonomic levels (order, family) to specific ones according to expertise on board. For some taxa, sampling includes: up to 10 voucher specimens with a unique batch number; photos; tissue samples in 80% ethanol for DNA analysis (Barcoding and Phylogeny); 30 samples minimum for population genetics (for abundant species); sampling for isotopic measures; fish chromosomes preparations; primary fish cell lines and cryopreservation of fish tissues for permanent cell lines The database was intended to contain information about stations, events, gear, all material collected and associated samples listed above. currently only contains information on material collected and samples. Data was recorded on log sheets then transcribed into an Oracle database called cabo. Tailor made user interace for entering data. No export functionality. SQL database dump has been provided but there was no-one on the voyage to elaborate on the structure, this was promised post voyage along with some simple data exports to match the log sheets, so we have access to the data without the unfriendly database.

Accompanying excel spreadsheet details the DNA samples taken and venom glands obtained and associated date/location/depth collection site data. Each specimen will be DNA barcoded using the diagnostic 600bp region of the mitochondrial COI gene and sequences lodged on GenBank. DNA barcoding will aid in evaluating the extent of cephalopod biodiversity including revealing cryptic species and phylogeographic structure. Confirmation of species identities is also essential as glands from multiple individuals will be required for detailed proteomic analysis of the proteins produced by the octopus posterior salivary glands. The chemical composition of the secretions will be determined through the application of multidisciplinary techniques, including cDNA library construction, mass spectrometry, molecular modelling and bioactivity testing. The studies will commence in 2008 and run for several years, being the major element in a PhD project run through Dr. Fry's laboratory. Data_Format Excel file with the following columns: Sample ID Diagnosis Description Other Ref Gland Storage Tissue Tissue Storage Voucher Preservation Images Order Family Genus Species Location Site Depth (start-stop) Latitude Longitude Date Time (UT) Capture Event Other Information

Percent-cover estimates from forward facing still-images collected during the benthic trawls of the 2007/08 CEAMARC voyage (raw data-set here: https://data.aad.gov.au/metadata/records/CEAMARC_CASO_200708_V3_IMAGES). All fauna in the bottom third of each image was scored to the lowest taxonomic resolution possible. The images originate from 32 transects, but were split by their lon-lat-position within a spatial grid of environmental variables into 41 sites. This dataset contains: (1) - species/ morphotypes identified to the highest taxonomic resolution possible - broader taxonomic classification (phylum/class) - each species mobility, feeding-type and body-shape if possible - average abundances in percent-cover at each site (2) - the mean longitude of all images aggregated per site - the mean latitude of all images aggregated per site - the number of images scored per site

This dataset contains scanned copies of the RMT and bottom trawl logs from Voyage 6 1990-91 (AAMBER2) of the Aurora Australis. This was primarily a marine science voyage. Surveys of krill, other zooplankton and pelagic fish were taken in Prydz Bay, Antarctica between January and February 1991. 177 midwater trawls were successfully completed at 59 stations. Midwater fish were sampled using an International Young Gadoid Pelagic Trawl (IYGPT). At each station, hauls were taken at depths of 20-30m, approximately halfway down the water column, and 20-30m above the bottom. At six stations, the lowest sample was duplicated using a light fitted to the net. Where samples were made off the shelf, standard depths of 20-30m, 400m, and 800m were fished. All hauls were of 30 minutes fishing time. Bottom trawls were made using a 35m headline length otter trawl fitted with 40cm diameter bobbin gear. A 2" mesh cod end liner was used to retain small fish. On both nets, a Simrad trawl surveillance sonar was used.

This project aims to assess the vulnerability of and risks to habitats in Australian fisheries in the Australian Exclusive Economic Zone (EEZ)/Australian Fishing Zone (AFZ) of the Southern Ocean to impacts by different demersal gears - trawl, longline and traps. The project which is a collaborative initiative between the Australian Antarctic Division (AAD), the Australian Fisheries Management Authority (AFMA), industry and research partners, and substantially funded by the Fisheries Research and Development Corporation, was developed in order to resolve outstanding questions relating to the potential impacts and sustainability of demersal fishing practices in the AFZ at Heard Island and the McDonald Islands (HIMI). It will also help resolve similar outstanding questions for other fisheries in the Commission for the Conservation of Antarctic Marine Living Resources (CCAMLR) in which Australian industry participates and provide technology for use in other fisheries to address similar questions. The proposed project will assess the degree to which demersal gears interact with and possibly damage benthic habitats. It will also assess the degree to which these habitats might be damaged within the AFZ in the HIMI region. The project is not intended to estimate rates of recovery of benthic habitats following damage by demersal gears. However, information from the literature on rates of recovery of different benthic species and habitats will be used to assess the risks of long-term sustainability of these habitats. Objectives To develop deep sea camera technologies that can be easily deployed during fishing operations, to facilitate widespread observations of demersal fishing activities (trawl, longline and trap) and their interactions with benthic environments. To assess the vulnerability of benthic communities in Sub-Antarctic (Australian AFZ) and high latitude areas of the Southern Ocean (Australian EEZ) to demersal fishing using trawls, long-lines or traps, using video and still camera technologies. To assess the risk of demersal fishing to long-term sustainability of benthic communities in these areas, based on the assessment of vulnerability and information from the literature on potential recovery of benthic species and habitats. To recommend mitigation strategies by avoidance or gear modification, where identified to be needed, and practical guidelines to minimise fishing impacts on benthic communities. Field work: Field work for this project is well advanced. Sampling of benthic habitats was conducted off East Antarctica from the AA in the summer season of 2009/10. Sampling yielded biological samples and camera footage over a number of sites spread across a large section of the East Antarctic coast and across a range of benthic habitats, however sampling was limited by the extent of ice and number of ship days (10) allocated (the project was originally planned for 16 ship days and later in the summer, when ice was predicted to be less extensive). The camera units are currently deployed on commercial vessels fishing the sub-Antarctic. The close of the 2010 commercial fishing season in September 2010 will mark the conclusion of field activities for this project.

The Kerguelen Axis voyage was planned to collect data to enhance the realism of end-to-end ecosystem models being developed in the Antarctic Climate and Ecosystems Cooperative Research Centre, to investigate the effects of climate change and ocean acidification on Southern Ocean ecosystems in the Indian Sector (particularly in relation to factors affecting the northern distribution of Antarctic krill) and to contribute to assessment of the spatial relationship of mesopelagic mid-trophic level species, in particular zooplanktivores, to foraging strategies by marine mammals and birds on the Kerguelen Plateau. Nine projects were undertaken aboard the Aurora Australis. Each project had individual objectives and outputs, and there are metadata records for each data set collected. They were designed to be complementary in order that the whole data set and project analyses could be used to address the objectives of the Kerguelen Axis program. Observations will be contributed to the Southern Ocean Observing System (SOOS) and will facilitate the design of future ecosystem observing in the region.