Purpose of future metagenomic (DNA), metaproteomic (protein) and metatranscriptomic (RNA) analysis: For each sample, two drums (~200L each) of seawater were collected. Samples were taken from CTD sites, and surface samples (2m depth) taken at each of these sites. At most of these CTD sites, a deeper sample was taken according to the location of the DCM at that site. The 200L seawater is pumped through a 20 micron mesh to remove the largest particles, then the biomass is collected on three consecutive filters corresponding to decreasing pore size (3.0 microns, 0.8 microns, 0.1 microns). This is repeated for each sample using the second 200L of seawater to generate duplicates for each sample. The overall aim is to determine the identity of microbes present in the Southern Ocean, and what microbial metabolic processes are in operation. In other words: who is there, and what they are doing. Special emphasis was placed on the SR3 transect. Samples were collected as below. For each sample, a total of six filters were obtained (3x pore sizes, 2x replicates). Each filter is stored in a storage buffer in a 50mL tube, and placed at -80 degrees C for the remainder of the voyage.

FRRF deployments were conducted at 22 sites in conjunction with ship stop times when the CTD was deployed. See event log for locations. Some underway FRRF sampling was conducted on the return voyage. This work was conducted as part of the VMS (Voyage Marine Science) voyage of the Aurora Australis in the 2010-2011 season. A report providing further details about the FRRF work is available as part of the download file. The download file also contains a word document (also included in the download file for metadata record ASAC_1307) explaining the data columns in the excel spreadsheets.

Oceanographic measurements were collected aboard Aurora Australis cruise au1121, voyage "Marine Science" (i.e. voyage 2.1) 2010/2011, from 4th January to 6th February 2011. The cruise commenced with a full north to south occupation of the CLIVAR/WOCE meridional repeat section SR3, followed by work around the Antarctic continental margin in the region of the Adelie Depression and the former Mertz Glacier ice tongue. A total of 149 CTD vertical profile stations were taken on the cruise, most to within 15 metres of the bottom. Over 2000 Niskin bottle water samples were collected for the measurement of salinity, dissolved oxygen, nutrients (phosphate, nitrate+nitrite and silicate), oxygen-18, dissolved inorganic carbon (i.e. TCO2), alkalinity, pH, helium, tritium, and biological parameters, using a 24 bottle rosette sampler. Upper water column current profile data were collected by a ship mounted ADCP. Meteorological and water property data were collected by the array of ship's underway sensors. An array of 3 bottom mounted ADCP moorings were deployed near the Adelie Depression, for recovery in the 2012/13 season. Underway data were also collected on this voyage, and are linked to this metadata record at the provided URL. A detailed readme is available as part of the download. Finally, ADCP (Acoustic Doppler Current Profiler) data are also linked, and are in Matlab format.

Geoscience Australia and the Australian Antarctic Division conducted a benthic community survey using underwater still photographs on the shelf around the Mertz Glacier region. The purpose of the work was to collect high resolution still photographs of the seafloor across the shelf to address three main objectives: 1. to investigate benthic community composition in the area previously covered by the Mertz Glacier tongue and to the east, an area previously covered by fast ice 2. to investigate benthic community composition (or lack thereof) in areas of known iceberg scours 3. to investigate the lateral extent of cold water coral communities in canyons along the shelf break. Benthic photos were captured using a Canon EOS 20D SLR 8 megapixel stills camera fitted with a Canon EF 35mm f1.4 L USM lens in a 2500m rated flat port anodised aluminium housing. Two Canon 580EX Speedlight strobes were housed in 6000m rated stainless steel housings with hemispherical acrylic domes. The camera and strobes were powered with a 28V 2.5Ah cyclone SLA battery pack fitted in the camera housing and connected using Brantner Wetconn series underwater connectors. The results were obtained with 100 ASA and a flash compensation value of +2/3 of a stop. The focus was set manually to 7m and the image was typically exposed at f2.8 and a shutter speed of 1/60 sec. The interval between photos was set to 10 or 15 seconds. The camera was fitted to either the CTD frame or the beam trawl frame and lowered to approximately 4-5 m from the bottom. Two laser pointers, set 50 cm apart, were used for scale. The camera was deployed at 93 stations, 7 using the beam trawl frame and 86 using the CTD frame. The stations were named by: 1. Camera deployment frame (e.g. CTD or beam trawl, BT) 2. Frame sequence number (e.g. CTD53) 3. Instrument (e.g. camera = CAM) 4. Sequence of camera deployments through the survey overall (e.g. first deployment = CAM01, second deployment = CAM02 etc). For example, BT5_CAM16 is the sixteenth camera deployment of the survey overall, and was the fifth deployment using the beam trawl frame. From the 93 stations, there were 75 successful camera deployments. There were no photos captured at 9 stations. This was due to the camera or strobes malfunctioning, the camera being too far from the bottom, or the camera or strobes being in the mud at the bottom. The photos at a further 9 stations are considered poor due to the camera being out of focus, the camera being a little too far from the bottom or because very few photos were captured of the bottom. The benthic photo will be used to document the fauna and communities associated with representative habitats in the study area. The post-cruise analysis of the benthic photos will involve recording seabed geology and biology (class or order, and whatever is significant for the habitat) for each image