Dimethylsulfide and its precursors and derivatives constitute a major sulfate aerosol source. This dataset incorporates the potential for increased UV radiation effects due to stratospheric ozone depletion over spring and summer in Antarctica, using large-scale incubation systems and 13-14 day incubation periods. Surface seawater (200 micron filtered) from the Davis coastal embayment was incubated during four experiments over the 2002-03 Antarctic Summer. The data incorporates seawater measurements of DMS, DMSP and DMSO over a temporal progression during each incubation experiment. Six polyethylene tanks of varying PAR and UV irradiances were incubated. Water was collected stored and analysed by gas chromatography according to a specific sampling protocol, employed by all investigators associated with the project. The data are organised according to analysis day, with each days calibration data displayed at the top of each sheet. The sample code is followed by GC run number and then the raw count data from the GC. This is calculated to nanomoles DMS, DMSP or DMSO. Sample Codes: Codes for temporal data follow format X.XXXX 1st X gives experiment number, 1 to 4. 2nd X gives sampling day, 0, 0.5, 1, 2, 4, 7, 14 (will result in digit code for day no. less than 10 3rd X gives tank number relating to irradiance level(one to six) 4th and 5th X is replicate number, (01, 02, 03, DMS), (04, 05,06, DMSP total), (07, 08, 09, DMSP dissolved), (10, 11, 12, DMSO total). The fields in this dataset are: Sample Code Run Number from the GC Counts - GC generated raw data Log Counts - logarithmic conversion of the count data Log -c - logarithmic conversion minus the y-intercept determined by calibration of the GC. (log -c)/m - log -c divided by m, determined by calibration of the GC. ngS anti log - nanograms of Sulfur NaOH - NaOH adjustment ngS/L - adjustment per litre nM-DMSP/L - nanoMol's DMSP per litre nm-DMS/L - nanoMol's DMS per litre September 2013 Update: DMSO was analysed in these experiments according to an adaptation of the sodium borohydride (NaBH4) reduction method of Andreae (1980). The method has since been superseded and the data here probably displays inaccuracies as a result of the analytical method used. This DMSO data should be treated with caution.

Metadata record for data from ASAC Project 2297: Iron content of Southern Ocean phytoplankton: implications for carbon transfer to the deep sea. Data on size-fractionated distribution of suspended trace elements (including iron) in marine particles taken from the surface Southern Ocean south of Australia. Data for 4 size-fractions at 4 stations along ~142 degrees E are included. Explanation of codes used in the dataset: The isotope of the element of interest is listed down column A. LR refers to low resolution and MR medium resolution (that is the resolution of the ICPMS analytical instrument). So Mn55(LR) is the manganese isotope 55 ran in low resolution. SFP1_2um_B2_1: SFP=size-fractionated particles 1=station 1 2um=2micron filter size B2=blank 2 1=replicate 1 RSD%=relative standard deviation in % Blank=field blank Blk/sample%=blank-to-sample ratio in % CRMs_261102=certified reference materials (ran on 26 Nov 2002) Scaled up=previous column times multiplication factor (a serial dilution was used) Blk=blank subtracted certified=the value certified by the manufacturer of the reference material SLRS_1in25_1= the CRM 'SLRS', ran with a 1 in 25 dilution factor, replicate 1 DigBlk1_1=digestion blank 1, replicate 1 See the link below for public details on this project.

The data set includes information relevant for the study and description of sea-ice bacteria contains the following dataset subgroups and is organised by REFERENCE number. 1) Isolation data: strain designations (e.g. culture collection names are indicated for type cultures); media used for isolation and routine cultivation; temperature used for incubation; any special conditions (e.g. enrichment conditions) used for isolation; isolation site and type (e.g. sea-ice); availability of the indicated strain from the chief investigator (J. Bowman) 2) Phenotypic data: Includes morphological, physiological and biochemical tests performed. Details on how these were performed are indicated in the relevant reference. 3) Growth/temperature data: data for temperature related growth curves are given where available. Methods are indicated in the associated reference. 4) Fatty acid/chemotaxonomy data: fatty acid and other related data are given where available. Methods are indicated in the associated reference. 5) Genotypic data: data for DNA-guanosine/cytosine-content and genomic DNA:DNA hybridization are shown where available. Methods are indicated in the associated reference. 6) Phylogenetic data: data for sequences are cross-referenced to the GenBank database. In some cases, aligned sequence datasets are available in FASTA format and can be viewed in the programs BIOEDIT (www.mbio.ncsu.edu/BioEdit/bioedit.html) or CLUSTAL W (www.ebi.ac.uk/clustalw). 7) Other related published references which are useful or relevant to the dataset e.g. related sequences published subsequent to the ASAC study

Preliminary Metadata record for data expected from ASAC Project 1343 See the link below for public details on this project. Comparative study of the processes controlling carbon export in Southern Ocean environments characterised by a different hydrodynamical and ecological functioning. Work on this project was carried out on Voyage 3 of the Aurora Australis (CLIVAR) of the 2001 and 2002 season. Work at sea target sampling sites were the 8 'particle stations' along the CLIVAR SR3 repeat transect: the SAZ at 47 degrees and 49 degrees S; the SAF at 51 degrees S; the PFZ at 54 degrees S; the IPFZ at 57 degrees S; the SPZ at 59 degrees and 61 degrees S; the SACCF at 63 degrees S and the SSIZ at 64 degrees S. Some of these (64 degrees, 61 degrees and 51 degrees S) were sampled again on the way back to assess temporal evolution. All proxy studies (new production; Ba; delta30Si; 234Th-deficit) were done at each particle station but not necessarily on the same CTD casts. New production assessment Surface water (at 5, 25, 50 and 70m) was sampled with the CTD rosette at all particle stations. Different aliquots of 1L seawater were spiked with 15N-nitrate, 15N-ammonium or 15N-urea. All samples were spiked with 13C-bicarbonate; the latter in order to assess net primary production rates. Incubations (12 H) were done in a thermo stated algal cabinet, using appropriate neutral density screens for samples from depths below 5m. The samples were submitted to a constant light flux of 0.7x10power16 quanta/cm2/sec. Furthermore, samples from 5m depth were amended with increasing doses of ammonium (+0.1 micro M; +0.25 micro M; +0.5 micro M and +1 micro M) having natural 15N/14N abundance to assess susceptibility of N-uptake (ammonium, nitrate, urea) to ammonium. Similar experiments were run for three iron amended and control cultures in collaboration with Pete Sedwick, Dave Hutchins and Phil Boyd. Analysis of ammonium related to the incubation work was done on board by colorimetry. As a side product we obtained ammonium profiles at all particle stations and also six shallow CTD's in the southern part of the transect (greater than 61 degrees S). Suspended particle sampling for trace element analysis and isotopic composition of Si For biogenic-Ba was also carried out. Typically 14 depths were sampled between the surface and 1000m. On board filtration was performed on Nuclepore membranes. These were dried (60 degrees C) and stored for analysis in the shore-based lab. Occasionally, we also sampled large particles - size fractions (greater than 70 micro m and 20 less than 70 micro m) - from the upper 150m for Ba, using the bow pump system of Tom Trull. Ba and Sr incubations on large settling particles sampled with the Snatcher were also performed at 5 particle stations. For delta30Si, all 24 depths of the deep CTD casts at the particle stations 1 to 8 were sampled. Filtered seawater and suspended matter filtered on Nuclepore membranes (dried at 60 degrees C) were saved for later analysis in the home based laboratory. 234Th work - we refer to the report by Ken Buesseler for the major part of this work. In addition we performed some work using the 'Snatcher' Large Volume sampler and sedimentation column. Total 234Th deficit and 234Th activity on particles and solution was assessed at T0 and T4 H after return of the sampling device on board, in an attempt to construct the 234Th mass balance and eventually get at the settling speed (and flux) of 234Th carrying particles. These analyses went together with flow cytometry analyses (collaboration with Clive Crossley) to check for sedimentation by (fluorescent) particles and also with POC and biogenic silica in order to determine the elemental ratios of suspended and sinking particles. Flow cytometer results did not indicate there was significant sedimentation of life cells going on at this time of the year. Dissolved Ba Seawater samples were taken at all depths sampled by deep CTD's during the southward transect. Samples were acidified and kept for later analysis of dissolved barium by isotope dilution ICP-MS. Comparison of the dissolved Ba distribution along the transect with the one reconstructed through a multiple end-member mixing model will help understanding of the relative contribution of in-situ processes (uptake, dissolution) versus conservative mixing, thus improving our understanding of the oceanic Ba biogeochemistry. Analysis New production. Isotope ratio analysis of the 15N and 13C spiked natural plankton samples will be conducted in the home lab., using emission spectrometry and mass spectrometry. Mass balance calculations will allow assessing relative importance of new production as well as the fraction of new production that is in the particulate form and represents the potential for export. Ba and trace elements. Suspended matter samples will be acid digested (HNO3, HCl, HF) and analysed per ICP-MS and ICP-AES for contents of Ba, Ca, Sr, Al, Fe, Mn, Th, U, REE, Ti. The vertical concentration profiles will inform on the latitudinal and temporal variability of the biogeochemical control processes between SAZ, PFZ, ACC and SSIZ subsystems. For the sites with sediment trap deployments, particulate trace element distributions in the water column will be compared with trace element composition of fast settling particles intercepted by the traps. Ba-uptake / barite formation. Isotope ratio analysis (135Ba/138Ba; 86Sr/87Sr) of suspended matter incubated after spiking with 135Ba and 86Sr will be analysed by ICP-MS to investigate on the barite formation process. Abundance and type of barite crystals will be studied by SEM-EMP (mapping + photographs). delta30Si, In the home based lab. particle samples will be extracted using base (NaOH). Silicates in filtered seawater will be precipitated and analysed using a multi collector ICP-sectorial Mass Spectrometer (MC-ICP-MS) once this new method is set up. 234Th. Total, particulate and dissolved 234Th measurements were performed on board using low beta counters. Background (after 6 months decay) and chemical yields will be measured at Ken Buesseler's lab (WHOI, USA), using beta counters and ICP-MS respectively. The worksheets contained in the excel spreadsheet are: Phyo biomass New production and cell counts Particulate barium Dissolved barium d29Si isotope signature of dissolved silicic acid The fields in this dataset are: Carbon Seawater CLIVAR temperature pressure salinity depth barium latitude longitude oxygen silicate phophate nitrate flagellates diatoms picoplankton plankton urea ammonia coccolithophores