The productivity of Antarctic waters may be controlled by the amount of iron. Experiments have shown that this is probably the case for phytoplankton but as yet we do not know if iron limits the growth of sea ice algae. This study will assess whether iron limits sea ice algae production and will conduct experiments to work out how these algae use iron. Measurements have been made to determine whether sea ice algae are limited by Fe. Sea ice samples were taken and this spreadsheet refers to those ice cores Columns A-G are self explanatory Column G is the depth in the ice core from the bottom Column H is the chlorophyll concentration in mg Chl m-2 Column I is the phaeophytin concentration in mg m-2 J is the total amount of protein in the sample ng m-2 K is the total amount of the protein flavodoxin ng m-2 L is the total amount of ferrodoxin ng m-2 These last two enable the Fe limitation status to calculated (not completed).

Blood samples from 35 seals have been collected for serum biochemistry analysis. Scats from 20 animals have been collected for parasitology. Estimated weights and morphometric measurements from 35 animals have been collected. The data for this project are presented in a number of excel worksheets. In addition, a word document is also included in the download file which fully explains each spreadsheet. A precis of that word document is copied below. Haematology Data from haematological analysis performed on fresh whole blood collected from leopard seals between 27.12.1999-22.01.2002 and Weddell seals between 02.01.2001-21.01.2002 off Davis Station in the Austral summer seasons of 1999/2000, 2000/2001 and 2001/2002. Serum protein electrophoresis (SPE) Data from SPE analysis performed on serum (stored at -80 degrees C) collected from leopard seals between 27.12.1999-22.01.2002 and Weddell seals between 02.01.2001-21.01.2002 off Davis Station in the Austral summer seasons of 1999/2000, 2000/2001 and 2001/2002. Serum Biochemistry Data from biochemistry analysis performed on serum (stored at -80 degrees C) collected from leopard seals between 27.12.1999-22.01.2002 and Weddell seals between 02.01.2001-21.01.2002 off Davis Station in the Austral summer seasons of 1999/2000, 2000/2001 and 2001/2002. Trace element and heavy metal analysis Data from trace element and heavy metal analysis performed on serum (-80 degrees C), fur, frozen (-20 degrees C) and formalin (10%) fixed tissues, plasma (-80 degrees C), EDTA plasma (-80 degrees C), washed red blood cells (-80 degrees C) and urine (-20 degrees C) collected from leopard seals between 27.12.1999-17.02.2002 and Weddell seals between 02.01.2001-21.01.2002 off Davis Station in the Austral summer seasons of 1999/2000, 2000/2001 and 2001/2002 using inductively coupled plasma mass spectroscopy. The spreadsheet is organised into six worksheets: 1.Serum (micro g/L) 2.Fur (micro g/g dry weight) 3.Frozen tissues (micro g/g dry weight) 4.Plasma and RBC (red blood cells) (micro g/L) 5.Urine (micro g/L) 6.Formalin tissues (micro g/g dry weight) Faecal Parasites Data from faecal flotation in saturated salt solution performed on fresh and frozen (- 20 degrees C) scats collected from leopard seals between 23.11.1999-17.02.2002 and Weddell seals between 06.12.2000-16.01.2002 off Davis Station in the Austral summer seasons of 1999/2000, 2000/2001 and 2001/2002. The fields in this dataset are: Glucose Urea Creatinine Fibrinogen Protein Albumin Globulin Bilirubin ALP AST ALT Creatinine Kinase Cholesterol Calcium Phosphate Sodium Potassium Chloride Bicarbonate Anion Gap Amylase Lipase Date Faeces Cestode eggs Ascarid Eggs PCV WCC Neutrophil Eosinophil Basophil Lymphocyte Monocyte Band Neutrophil Serum Magnesium Aluminium Vandium Chromium Manganese Iron Cobalt Nickel Copper Zinc Arsenic Selenium Cadmium Mercury Lead Bismuth Date