Biopsy samples (small amounts of skin and blubber) were collected from humpback and blue whales during the Antarctic blue whale voyage 2013. For more information please see: http://www.marinemammals.gov.au/sorp/antarctic-blue-whale-project This dataset consists of an access database summarising biopsy events containing the fields: Pod: a combination of date and where the biopsy was taken from (small boat - Remora, or main vessel - Amaltal Explorer) Ship reference: sighting number Number of individuals sighted Age class and sex Satellite tag number Biopsy sample number Biopsy collection location (longitude and latitude) Biopsy collection time (UTC) Reaction to biopsy Number of biopsy attempts Photo ID images Biopsy collection photo Comments An excel spreadsheet (Biopsy_info_ ABWV2013.xlsx) details the biopsy processing that occurred upon collection of a sample. Where possible, each blue whale sample was split and preserved in 2 x All Protect, 1 x EtOH and 1 x Nitrogen. Each humpback sample was additionally preserved in RNA later and stored at -20 degrees Celsius and individually detailed in spreadsheet Biopsy_info _ABWV2013(hbw).xlsx. Biopsy samples were processed to determine sex and relatedness using DLOOP analysis and the results are held in: 240513 BW Sexing Results.xls

Biopsy samples were collected from humpback (n=10) and blue whales (n=1) during the NZ/Aus Antarctic Ecosystems Voyage 2015. Biopsy collection from humpback and blue whales was attempted from the bow of the ship using Larsen rifles. Biopsying blue whales from the bow of the RV Tangaroa proved challenging due to the ship’s manoeuvrability and the limited capacity to change speed rapidly. Biopsy samples were split between All Protect (Qiagen), 70% ethanol, and freezing at -20C. . This dataset consists of an excel spreadsheet (biopsy_events.xlsx) summarising biopsy events containing the fields: Date_taken (in UTC) Location (general) where sample collected Latitude Longitude Individual ID Sample ID Name of sampler Sample type Preservative used Species sampled An excel spreadsheet (Biopsy sample info datasheet AEV 2015.xlsx) details the biopsy processing that occurred upon collection of a sample. Where possible, each sample was split and preserved in 2 x All Protect, 1 x EtOH and 1 x -80 degrees Celsius. Samples preserved in All Protect and 70% ethanol are stored at the Australian Antarctic Division and samples preserved at -80C are stored at NIWA Wellington. A subsample of the Antarctic blue whale biopsy was submitted to the IWC-recognised genetic repository for Antarctic blue whale at NOAA Southwest Fisheries, La Jolla. Biopsy samples were processed to determine sex and the results are held in: TAN1502_Whale biopsy samples.xls