Two 16S rDNA clone libraries, one from a Brown Bay sample and one from an O'Brien Bay sample were generated. These samples were originally collected as part of ASAC project 868 and the microbiology of the samples is now being investigated as part of ASAC 1228. Two data files are included in the download. Both are in "fasta" format, a text-based format for representing either nucleotide sequences or peptide sequences, in which base pairs or amino acids are represented using single-letter codes. Further information about the dataset can also be found in the referenced paper.

Data shows carbon and nitrogen stable isotope concentration in siphon tissue of laternula elliptica from three sites adjacent to Casey Station. McGrady Cove, Brown Bay Inner and Shannon Bay. All shellfish were collected by divers during the 2014/15 summer season. Samples were sent to Cornell University Stable Isotope laboratory for analysis.

Data shows gross body measurements of lantern shellfish (Laternula elliptica) collected by divers at McGrady Cove; Brown Bay Inner, and Shannon Bay. Measurements include length, width, and height of shell and weight with shell on and shell off.

Gross body measurements of fish length (cm), weight (g), and sex (M/F). Fish were collected on line and in box traps at Brown Bay, Shannon Bay, near Wilkes Station, O'Brien Bay and Sparkes Bay. Sex was determined after dissection for other analyses.

Data show results of carbon and nitrogen stable isotopes in muscle tissue of Trematomus bernacchii collected at 5 sites adjacent to Casey Station. Sites are contaminated Brown Bay, near Wilkes Station, Shannon Bay and reference O'Brien Bay and Sparkes Bay. Approximately 1cm3 of muscle tissue from the left side of each fish was taken for stable isotopes analysis.

Sediment samples which were originally collected as part of ASAC 868 (ASAC_868) are now being investigated using molecular microbial techniques as part of ASAC 1228 (ASAC_1228). Samples were collected in a nested survey design in two hydrocarbon impacted areas and two unimpacted areas. Denaturing gradient gel electrophoresis (DGGE) of a region of the 16S RNA gene was used to investigate the microbial community structure. Banding patterns obtained from the DGGE were transformed into a presence / absence matrix and analysed with a multivariate statistical approach. The download file contains an excel spreadsheet, a csv version of the data, plus a readme file.

A survey of the epibenthic fauna of hard-substrata was made around Casey Station, using a camera mounted on a frame to take photoquadrats. A nested sampling design was used with several spatial scales. At each location there were two sites, approximately 50-100 m apart. At each site there were three by 15 m long transects, approximately 5 m apart. On each transect there were 2 plots, 5 m long by 2 m wide. Six photoquadrats were taken in each plot. This metadata record describes one component of this survey, photoquadrats taken in and around boulders and boulder fields. One of the aims of this survey was to determine if there is any evidence of human impacts in these assemblages. There were several control and several potentially impacted locations. The data have yet to be analysed. Also links to ASAC 1100.

The effect of location, depth and sediment contamination on recruitment of soft-sediment assemblages were examined in a pilot experiment at Casey Station, East Antarctica. Two locations were used, a polluted bay adjacent to an old disused tip site (Brown Bay) and an undisturbed control (O'Brien Bay). At each location two types of defaunated sediment (polluted and control) were placed at 2 depths, 15 m and 25 m. Sediments were left in place over the Austral winter, from March - November. There were large differences in recruitment between the two locations and depths and some differences between the two sediment types. Brown Bay had greater recruitment than O'Brien Bay. Shallow sites had generally greater recruitment than deep, but deep sites had greater diversity (H'), richness (d) and evenness (J'). Control sediment recruited greater numbers of arthropod, gammarid and isopod taxa. There were not only differences in abundance of taxa and assemblage structure but also in spatial variability and variability of populations of certain taxa, with recruitment to the control and deep locations more variable, and recruitment in the control sediment more variable than the polluted sediment. Recruitment was influenced by a combination of location, depth and sediment type. There is some evidence of an environmental impact at the polluted site. The majority of fauna recruiting to the experiment were highly motile colonizing species with non-pelagic lecithotrophic larvae, usually brooded and released as dispersing juveniles, such as gammarids, tanaids, isopods and gastropods. A total of 56 recruitment samples were collected. Samples were sieved at 500 micro metres and sorted mainly to species. Metal concentrations and total organic carbon concentrations are also included. Also links to ASAC 1100. The fields in this dataset are: Species Location Site Treatment (tmt) Site and replicate Toxicity Arsenic Cadmium Copper Lead Silver Zinc

A survey of macrobenthic assemblages in soft-sediments was done at Casey Station, East Antarctica. Samples were taken along four transects in Brown Bay, along a pollution gradient that has its source at an abandoned waste tip on the shore of Brown Bay. The transects were up to 300 M long and there were 9 sampling stations on each transect except for transect four which had only 4 sampling stations. Sampling stations were at the following distances from the shoreline and tip site: 10 m, 20 m, 30 m, 40 m, 50 m, 100 m, 150 m, 200 m, and 300 m. Two cores of the soft-sediment assemblages were taken at each sampling station and two cores for analysis of heavy metals and hydrocarbons. Samples were taken by divers using hand-held corers (fauna core size - 10 cm diameter by 10 cm deep; sediment analysis cores - 5 cm diam. by 10 cm deep). The aims were: 1) to determine the nature and extent of the contamination gradient in front of the tip site and; 2) to determine if there was a corresponding response in the soft-sediment assemblages along the contamination gradient. A total of 62 samples of soft-sediment assemblages and 62 samples for heavy metal and hydrocarbon analysis were taken. Sediment grain size was also examined at each sampling point. The benthic diatom communities were also analysed in each sample. Links to ASAC 1100. The fields in this dataset are: Distance Position Site and replicate Species

Marine soft-sediment assemblages were sampled from shallow (5 - 35m) nearshore regions around Casey Station, Windmill Islands, East Antarctica in late summer (Feb-March) 1997, using a van-Veen grab (surface area 20 x 25 cm). Samples were sieved through a 1 mm mesh and sorted to species where possible. A hierarchical, spatially nested sampling design was used with locations (km's apart), sites (100s of metres apart), and plots (10s of m). Two potentially impacted, polluted locations (adjacent to a sewage outfall and an old garbage tip) were compared with two control locations. Data was analysed using both multivariate and univariate statistical methods. Significant differences in assemblages were found between locations and between sites within locations. Significant differences in the abundances of taxa at several taxonomic levels (species, family, order, phylum) were found at all three spatial scales. Significant differences were also detected between the polluted and control locations. Compared with other Antarctic locations, the assemblages were dominated by crustaceans (90 to 97 % of individuals) and there was a paucity of polychaete fauna at the locations sampled. This study represents the first description of benthic assemblages from this region. A total of 70 samples are included in this data set. Also links to ASAC 1100. The fields in this dataset are: Location Site Plot/replicate Weight Volume Species