Metadata record for data from AAS (ASAC) project 3051. Public Environmental change is by far one of the major crises facing our planet in recent times. This project will contribute specifically to understanding the effects of climate change and other human-induced impacts on marine species in Antarctica. Through studying key ecological and biological processes in marine benthic invertebrates we will better understand the spatial scale of populations, the nature of the processes that maintain those populations, how environmental change will affect those processes, and the levels of genetic diversity and resilience in Antarctic marine communities. Taken together this information will enable better, more informed management of Antarctic marine ecosystems. Project objectives: The project objectives, as stated in the project application round 2008/09, appear below: This project will combine experimental tests of demographic change with genetic tests of population isolation and diversity to enable predictions of the resilience of Antarctic marine invertebrates to current and predicted environmental change. The specific objectives of the project are; 1. Effects of change. Understand the effects of environmental change on reproduction (fecundity, reproductive success) and the early life history (larval behaviour, survivorship, and recruitment) of key Antarctic marine benthic invertebrates. 2. Isolation. Determine the degree of isolation/connectivity among populations as well as the levels of genetic diversity of key Antarctic marine benthic invertebrates. 3. Resilience. Assess the resilience (ability to cope with or adapt) of Antarctic marine benthic invertebrates to environmental change. 4. Practical Outcomes. Develop improved predictive capacity to contribute towards the development of management strategies for the conservation of Antarctic marine benthic invertebrates. Taken from the 2008-2009 Progress Report: Progress against objectives: This project commenced in 2008/9. Objective 1 - Effects of change - Collected live echinoderms (Abatus spp, Sterechinus numeyeri, Diploasterias) from around Casey Station and transported these on the A319 back to Kingston. A preliminary fertilisation trial has been run using Sterechinus individuals, and the remaining individuals are now being maintained in aquaria for future reproductive studies. Objective 2 - Isolation - Tissue samples from over 200 Sterechinus numeyeri were collected from 5 sites around Casey Station. These will form the foundation for genetic connectivity studies, and will complement exisiting Abatus samples from the same location. Laboratory processing of these samples has commenced, and development of microsatellite markers for both species is underway. Objectives 3 and 4 represent late-stage components of the project, so no progress can be reported on these at this stage. Taken from the 2009-2010 Progress Report: Progress against the objectives: Objective 1 - Effects of change - Collected live urchins (Abatus spp and Sterechinus numeyeri) from around Davis Station. Ran a series of spawning trials, although these were largely unsuccessful, with most individuals having spawned prior to the beginning of the season. We ran one successful fertilisation trial with S. neumeyeri to look at the effects of water temperature and salinity on fertilisation success. Preliminary analysis of the data indicates these environmental parameters do have an effect on fertilisation. Objective 2 - Isolation - Tissue samples from over 350 Sterechinus numeyeri were collected from 12 sites around Davis Station. These will be used for genetic connectivity studies, and will complement samples collected from Casey in the previous season. Larval Sterechinus were also collected from the water column and preserved for genetic analysis along with adult and juvenile Abatus ingens. Microsatellite markers (11 polymorphic loci) have now been developed for Sterechinus, and microsatellite deveopment is partially completed for Abatus ingens; the library has been created but optimisation of loci still needs to be done. We have completed DNA sequencing for Sterechinus and Abatus from Casey Station for 1 gene region (16S) and are optimising an additional 2 regions. This will be used to compare populations from Davis and Casey to understand large-scale connectivity. Objectives 3 and 4 represent late-stage components of the project. As this is only the second year of a 5-year programme, no progress can be reported on these at this stage.

This metadata record is a 'Parent' metadata record for ASAC project 2720. See the link for the related 'Child' metadata records. The overall objective is to characterise Southern Ocean marine ecosystems, their influence on carbon dioxide exchange with the atmosphere and the deep ocean, and their sensitivity to past and future global change including climate warming, ocean stratification, and ocean acidification from anthropogenic CO2 emissions. In particular we plan to take advantage of naturally-occurring, persistent, zonal variations in Southern Ocean primary production and biomass in the Australian Sector to investigate the effects of iron addition from natural sources, and CO2 addition from anthropogenic sources, on Southern Ocean plankton communities of differing initial structure and composition. SAZ-SENSE is a study of the sensitivity of Sub-Antarctic Zone waters to global change. A 32-day oceanographic voyage onboard Australia's ice-breaker Aurora Australis was undertaken in mid-summer (Jan 17 - Feb. 20) 2007 to examine microbial ecosystem structure and biogeochemical processes in SAZ waters west and east of Tasmania, and also in the Polar Frontal Zone south of the SAZ. The voyage brought together research teams from Australasia, Europe, and North America, and was led by the ACE CRC, CSIRO Marine and Atmospheric Research, and the Australian Antarctic Division. The overall goal is to understand the controls on Sub-Antarctic Zone productivity and carbon cycling, and to assess their sensitivity to climate change. The strategy is to compare low productivity waters west of Tasmania (areas with little phytoplankton) with higher productivity waters to the east, with a focus on the role of iron as a limiting micro-nutrient. The study also seeks to examine the effect of rising CO2 levels on phytoplankton - both via regional intercomparisons and incubation experiments. Available for download from this metadata record are various datasets collected from the voyage: - An image showing a map of the cruise track. - An excel document detailing hourly position checks of the ship. - An excel document detailing the event log for the voyage. - A word document detailing prospective papers produced from the voyage. Finally a link is available for users to access the special volume of publications produced as a result of this voyage.

Metadata record for data from ASAC Project 2720 See the link below for public details on this project. The overall objective is to characterize Southern Ocean marine ecosystems, their influence on carbon dioxide exchange with the atmosphere and the deep ocean, and their sensitivity to past and future global change including climate warming, ocean stratification, and ocean acidification from anthropogenic CO2 emissions. In particular we plan to take advantage of naturally-occurring, persistent, zonal variations in Southern Ocean primary production and biomass in the Australian Sector to investigate the effects of iron addition from natural sources, and CO2 addition from anthropogenic sources, on Southern Ocean plankton communities of differing initial structure and composition. These samples were collected on the SAZ-SENSE scientific voyage of the Australian Antarctic Program (Voyage 3 of the Aurora Australis, 2006-2007 season). SAZ-SENSE is a study of the sensitivity of Sub-Antarctic Zone waters to global change. A 32-day oceanographic voyage onboard Australia's ice-breaker Aurora Australis was undertaken in mid-summer (Jan 17 - Feb. 20) 2007 to examine microbial ecosystem structure and biogeochemical processes in SAZ waters west and east of Tasmania, and also in the Polar Frontal Zone south of the SAZ. The voyage brought together research teams from Australasia, Europe, and North America, and was led by the ACE CRC, CSIRO Marine and Atmospheric Research, and the Australian Antarctic Division. The overall goal is to understand the controls on Sub-Antarctic Zone productivity and carbon cycling, and to assess their sensitivity to climate change. The strategy is to compare low productivity waters west of Tasmania (areas with little phytoplankton) with higher productivity waters to the east, with a focus on the role of iron as a limiting micro-nutrient. The study also seeks to examine the effect of rising CO2 levels on phytoplankton - both via regional intercomparisons and incubation experiments. The data described in this metadata record are for seawater samples collected for HPLC pigments, microscopy and flow cytometry. Samples were collected either by Niskin Bottles (on a CTD), from the ocean surface with a bucket, or via a clean seawater line (at a depth of 7 metres), directly into the onboard laboratories. Samples for microscopy were examined either with an electron microscope, or a light microscope (lugol samples). The data are presented in an excel spreadsheet, available for download at the URL given below. The 'Notes' worksheet provides further information about the data contained in the spreadsheet, including a description of column headings, units used, etc. The fields used in this dataset are: Tube Label Site CTD Niskin bottle Depth (m) Date (UT) Start Time (UT) Stop Time (UT) Latitude Longitude Lugols Glutaraldehyde fixed samples Flow Coccolithophorids Volume HPLC Volume Turner Fluorometer reading (PAR) Photosynthetically Active Radiation Temperature (degrees C) Comment