This data set contains concentrations of phytoplankton, protozoa, total bacteria and metabolically active bacteria assessed by flow cytometry on transects 12, 1, 3, 5, 7, 9 and 11 of the BROKE-West survey of the Southern Ocean between January and March 2006. Only total bacterial concentrations were assessed for transect 11. Between 4 and 12 depths were sampled for marine microbes and concentrations were assessed using FACScan flowcytometer. Phytoplankton were identified and counted based on the autofluorescense of chlorophyll a when excited by the 488 nm laser of the FACScan. Protozoa were identified and counted after staining with the acid vacuole stain Lysotracker Green. Total bacteria were identified and counted using the cell permeant SYTO 13 nucleic stain. Metabolically active bacteria were identified and counted after staining for intracellular esterases with the esterase stain 6CFDA. The fields in this dataset are: Latitude Longitude Transect Number CTD number, flow file Depth (m) Total bacteria (per millilitre) Active bacteria (per millilitre) Dead bacteria (per millilitre) Protozoa (per millilitre) Phytoplankton (per millilitre) This work was completed as part of ASAC project 40 (ASAC_40).

Data acquisition Samples were collected at the 3 sites at 9 different depths. Depths included the bottom depth as well as 8 samples in the top 200M. 50ml samples were collected from the niskin bottles attached to the CTD. 20ml was filtered through a 0.02 microlitre filter to remove the viruses and larger organisms from the sample. 1ml of this virus free water was then pipetted into individual cryovials. A further 1ml was then taken from the original seawater sample and was then passed through a 0.2 microlitre filter, which retained all the bacteria and algae. The 0.2 microlitre filter was then placed in the cryovial with the 1ml of virus free seawater. Samples were then fixed with glutaraldehyde (5 microlitre, 50% concentration) at hourly intervals for 4 hours. After fixation samples were placed in liquid nitrogen and later stored at -80 degrees C. All samples will be analysed using a flowcytometer. The fields in these datasets are: Date Leg number Latitude Longitude Subsample Depth (m) Bottle Number Viscosity (cP) This work was completed as part of ASAC projects 2655 and 2679 (ASAC_2655, ASAC_2679).

Metadata record for data from ASAC Project 2720 See the link below for public details on this project. The overall objective is to characterize Southern Ocean marine ecosystems, their influence on carbon dioxide exchange with the atmosphere and the deep ocean, and their sensitivity to past and future global change including climate warming, ocean stratification, and ocean acidification from anthropogenic CO2 emissions. In particular we plan to take advantage of naturally-occurring, persistent, zonal variations in Southern Ocean primary production and biomass in the Australian Sector to investigate the effects of iron addition from natural sources, and CO2 addition from anthropogenic sources, on Southern Ocean plankton communities of differing initial structure and composition. These samples were collected on the SAZ-SENSE scientific voyage of the Australian Antarctic Program (Voyage 3 of the Aurora Australis, 2006-2007 season). SAZ-SENSE is a study of the sensitivity of Sub-Antarctic Zone waters to global change. A 32-day oceanographic voyage onboard Australia's ice-breaker Aurora Australis was undertaken in mid-summer (Jan 17 - Feb. 20) 2007 to examine microbial ecosystem structure and biogeochemical processes in SAZ waters west and east of Tasmania, and also in the Polar Frontal Zone south of the SAZ. The voyage brought together research teams from Australasia, Europe, and North America, and was led by the ACE CRC, CSIRO Marine and Atmospheric Research, and the Australian Antarctic Division. The overall goal is to understand the controls on Sub-Antarctic Zone productivity and carbon cycling, and to assess their sensitivity to climate change. The strategy is to compare low productivity waters west of Tasmania (areas with little phytoplankton) with higher productivity waters to the east, with a focus on the role of iron as a limiting micro-nutrient. The study also seeks to examine the effect of rising CO2 levels on phytoplankton - both via regional intercomparisons and incubation experiments. The data described in this metadata record are for seawater samples collected for HPLC pigments, microscopy and flow cytometry. Samples were collected either by Niskin Bottles (on a CTD), from the ocean surface with a bucket, or via a clean seawater line (at a depth of 7 metres), directly into the onboard laboratories. Samples for microscopy were examined either with an electron microscope, or a light microscope (lugol samples). The data are presented in an excel spreadsheet, available for download at the URL given below. The 'Notes' worksheet provides further information about the data contained in the spreadsheet, including a description of column headings, units used, etc. The fields used in this dataset are: Tube Label Site CTD Niskin bottle Depth (m) Date (UT) Start Time (UT) Stop Time (UT) Latitude Longitude Lugols Glutaraldehyde fixed samples Flow Coccolithophorids Volume HPLC Volume Turner Fluorometer reading (PAR) Photosynthetically Active Radiation Temperature (degrees C) Comment

This dataset contains results from the Second International BIOMASS Experiment II (SIBEX II) cruise of the Nella Dan, taken in January 1985. This cruise was the fourth cruise in a series of six. Phytoplankton samples were taken off Antarctica in the Australian sector (Mawson to Davis region) and Prydz Bay in January 1985. Taxonomic identity, distribution and abundance data were obtained, together with an extensive range of pigment analysis, using high performance liquid chromatography (HPLC). Over 60 pigments were analysed (only the major ones are listed here). The major phytoplankton investigated were diatoms, dinoflagellates and flagellates. This dataset is a subset of the full cruise. An excel spreadsheet containing the full pigment analysis obtained from the cruise is available for download from the URL given below. The spreadsheet is a digital version of the data presented in ANARE Research Notes 58, which was a report written based on this dataset. There are three worksheets to the spreadsheet: 1) Abbrev. - details the abbreviations used in worksheets 2 and 3. 2) Table 3 - Table 3 data entered from ANARE Research Notes 58. 3) Transposed Table 3 - The same data as worksheet 2, but arranged differently. A pdf copy of ANARE Research Notes 58 is also available for download at the URL given below. A paper written in 2006 about pigments in microalgae, which provides some up-to-date explanations about pigmentation, is also available for download, but owing to copyright restrictions, is only available for download by Australian Antarctic Division personnel. The fields in this dataset are: Date Time (GMT) Latitude Longitude Depth (metres) Pigment concentration (nanograms per litre) chlorophyllide a chlorophyll c methyl chlorophyllide a phaeophorbide a peridinin 19'-butanoyloxyfucoxanthin fucoxanthin 19'-hexanoyloxyfucoxanthin Neoxanthin Prasinoxanthin Violaxanthin Diadinoxanthin Alloxanthin diatoxanthin Zeaxanthin Canthaxanthin Unknown Chlorophyll b Chlorophyll a allomer Chlorophyll a Chlorophyll a epimer Phaeophytin a derivative Phaeophytin b Phaeophytin a Chlorophyll a total % Degradation Pigment total This work was completed as part of ASAC project 40 (ASAC_40).