Metadata record for data from AAS (ASAC) project 3046. Public The overall objective is to characterise the response of Southern Ocean calcareous zooplankton to ocean acidification resulting from anthropogenic CO2 emissions. Simulated increases in anthropogenic CO2 suggest a reduction in the calcification rates of calcareous organisms. A change in the calcification in the Southern Ocean may cause marine ecosystem shifts and in turn alter the capacity for the ocean to absorb CO2 from the atmosphere. We plan to take advantage of naturally-occurring, persistent, zonal variations in Southern Ocean primary production and biomass to investigate the effects of CO2 addition from anthropogenic sources on Southern Ocean calcareous zooplankton communities. A download file containing an excel spreadsheet of data can be found at the provided URL. Project objectives: The overall objective of this project is to characterise the impacts of recent, primarily anthropogenic, increases in atmospheric CO2 and related changes in the carbonate chemistry on shell formation by calcareous zooplankton in the Australian sector of the Southern Ocean. Calcareous zooplankton (e.g. planktonic foraminifera and pteropods) will be collected using plankton nets at five Southern Ocean localities during high seasonal flux periods. Planktonic foraminiferal and pteropod species and abundances, calcification rates and geochemistry (stable isotope and trace-metal) will be determined on plankton tow samples. Data from recent plankton tow samples will be compared with data deposited historically in the Southern Ocean and recovered from existing deep ocean sediment cores to provides insights about the extent to which modern carbon conditions may have already generated ecological impacts. The project will also provide a baseline of the present-day impact of ocean acidification and can be used to monitor the influence of future anthropogenic CO2 emissions in Southern Ocean ecosystems. Taken from the 2008-2009 Progress Report: Progress against objectives: Because of logistical delays to the Aurora Australis shipping schedule, ship time for this project was deferred to the 2009/2010 season. We have made progress in analysing other materials form previous voyages which will assist in the sampling design for the upcoming season. We are making good progress in planning the upcoming voyage currently scheduled for late 2009. Taken from the 2009-2010 Progress Report: Progress against objectives: Project scientists participated in Voyage 2 of the Aurora Australis, from Hobart to Casey Station in December 2009. Using the Rectangular Midwater Trawl we collected a total of eight plankton samples for examination of calcareous plankton distribution and shell characteristics in the summer Southern Ocean. We were targeting pteropods and planktonic foraminifera, two sets of calcifiers whose calcification response to ocean acidification we had previously reported on in publications in Nature Geoscience, Biogeosciences Discussions, and Deep-Sea Research Part II (in press). Project participants included collaborators from Australian National University and Scottish Natural Heritage, UK. There were low abundance of planktonic calclfiers in this particular seasons and sector, but we consider the initial collection a god start. Samples included approx. 18 pteropods; other samples are still being held by Biosecurity Australia and will be examined as soon as they are released. Other samples have already been sent to researchers at the Australian Institute of Marine Science for genetic (RNA) sequencing. This latter collaboration is a key one which will help answer questions about evolutionary responses to ocean acidification; if there are genotypes which are more or less vulnerable to acidification we may already be seeing selective pressure in the ecosystem and a change in the structure of assemblages as "winners" and "losers" are differentially affected by the impact.

This data set was collected from a ocean acidification minicosm experiment performed at Davis Station, Antarctica during the 2014/15 summer season. It includes: - description of methods for all data collection and analyses. - environmental data logged throughout the experiment; nutrients, temperature, light climate. - carbonate chemistry data; pH (on Total scale), fugacity of CO2, dissolved inorganic carbon concentration, practical alkalinity, Omega calculations for both araganite and calcite. - product datasheet (including transmission spectra) of Osram 150W HQI-TS/NDL metal halide lamps.

This data set was collected from a ocean acidification minicosm experiment performed at Davis Station, Antarctica during the 2014/15 summer season. It includes: - description of methods for all data collection and analyses. - marine microbial community data; Chlorophyll a concentration, particulate organic matter concentration (carbon and nitrogen), bacterial cell abundance. - phytoplankton primary productivity data; 14C-sodium bicarbonate incorporation raw data (decays per minute: DPM) and modelled productivity from photosynthesis versus irradiance (PE) curves, O2-evolution derived net community productivity, respiration, and gross primary productivity. - phytoplankton photophysiology data; community photosynthetic efficiency from PAM measurements (maximum quantum yield of PSII: Fv/Fm), PAM steady state light curve data and derived non-photochemical quenching of Chl a fluorescence (NPQ), relative electron transport rates (rETR), and effective quantum yield of PSII (delta F/Fm'). - phytoplankton carbon concentrating mechanism (CCM) data; maximum quantum yield of PSII (Fv/Fm) and effective quantum yield of PSII (∆F/Fm') from PAM measurements on size-fractionated phytoplankton samples (less than 10 microns and greater than 10 microns cells) exposed to; ethoxzolamide (EZA) which inhibits both intracellular carbonic anhydrase (iCA) and extracellular carbonic anhydrase (eCA), acetazolamide (AZA), which blocks eCA only, and a control (no inhibitor) sample. - bacterial productivity data; 14C-Leucine incorporation raw data (decays per minute: DPM) and calculated productivity.