Rapid toxicity tests (Kefford et al. 2005) were used to test the sensitivity of a wide range of intertidal and shallow sub-tidal marine invertebrates collected off the northern end of Macquarie Island. The tests were 10 days long, with a water change at 4 days. Resulted in the data set are non-modelled LCx (concentrations lethal to x% of the test populations) values for Copper (Cu) 10 days of exposure. Kefford, B.J., Palmer, C.G., Jooste, S., Warne, M.St.J. and Nugegoda, D. (2005). What is it meant by '95% of species'? An argument for the inclusion of rapid tolerance testing. Human and Ecological Risk Assessment 11: 1025-1046. Invertebrates collected from a range of coastal waters off the northern end of Macquarie Island . The columns in the spreadsheet are as follows: Lowest ID = the lowest identification the taxa is ID to (can be species, genus, family, etc.) Group = major taxonomic group the taxa comes from Letter = a convent identifier to split the taxa LC50 discpt = a string description of the10 day LC50 (lethal concentration for 50% of the test population) LC50 point estimate = a point estimate of the 10 day LC50 (lethal concentration for 50% of the test population) Cencor = indicates if the LC50 is right censored (that is greater than the value indicated in the point estimate) Case = a number to identify the record Project Public Summary: Despite pollution concerns in Antarctic and southern oceans, there is little ecotoxicological data and none from the sub-Antarctic. Ecological risk assessments and water quality guidelines should use local data, especially in the polar environment as organisms may respond differently to pollutants. The sub-Antarctic is, however, between Antarctica and the temperate zone and in the absence of local data, it maybe appropriate to use temperate data. This project will assess how the sensitivity to metals of marine invertebrates varies latitudinally and in which region of the Antarctic, if at all, it is appropriate to use temperate data.

The intention of the Deep Krill Camera and Trap System was to monitor and capture krill found during deep CTD operations. Two traps were installed on the CTD in place of Niskin Bottles. At pre-determined depths an internal light was illuminated and the traps were opened. After a set period of time a second trigger signal was sent to the traps, closing the entry point, encapsulating any Krill that were inside. The Krill Camera system was installed onto the CTD rosette. It consisted of a high-definition video camera (a GoPro Hero 2) within a pressure housing, flanked by two LED light sources. The power for this system was supplied via a rechargeable battery pack also mounted to the CTD. The camera system was remotely controlled from the surface via the CTD communications link. At specific depths the lights and camera were activated, recording the water column and ocean floor by adjusting focus length for fixed durations in an attempt to document Krill at lower depths. An additional camera was introduced into the system, mounted to allow video capture of the Krill Trap Operation. This camera was set to record at the beginning of the operations and left running for the duration of the deployment. Video data from the Krill camera is in MTS format, which can be opened with VLC Media Player. Trap footage is recorded in MP4 format, which can be opened with Quicktime or VLC Media Player. Trap triggering and camera operation data was recorded manually by Rob King.

This metadata record will contain the results of analyses of tissue samples from Antarctic Rock-cod (Trematomus bernacchii) collected at sites around Davis station to determine wastewater exposure and sub-lethal impact. AAS Project 4177. The results of metal analysis, stable isotope analysis and images of histological analysis of fish from Davis Station are in this dataset. Sample sites and fish collection Antarctic Rock-cod were collected at 6 sites from Prydz Bay near Davis Station East Antarctica, during the 2012/13 summer. Approximately twenty fish were collected from each site by line and in box traps from four sites along a (9 km) spatial gradient starting from the Davis Station wastewater outfall, southward 0km (within 250m of the point of discharge), 1km, 4km and 9km, in the direction of the predominant current. Additionally, two reference sites were sampled 9 km and 16 km north of the discharge point. Once collected, fish were immediately returned to the Davis Station laboratories and sacrificed individually by immersion in an Aqui-s solution (~15ml/L). Once no signs of life were present (approximately 5 min), fish length and weight were measured. Tissues were preserved in various ways for a number of analyses to be conducted at a later date. Stable Isotope analysis. Davis Station Laboratory Dorsolateral muscle tissue from the left side of each individual was removed, placed in aluminium foil and frozen at -20 degrees C for later analysis. Tissue processing A section of frozen tissue was removed (approximately 1 x 1 cm cubed), placed into a clean, acid washed glass crucible and cut into small pieces. This was then dried at 80 degrees C for 48 h. Tissue from each fish was carefully removed and placed into separate 2 ml Eppendorf tubes, each containing an washed, dried stainless steel ball bearing and the lids closed tightly to ensure no moisture could enter. Tissue was crushed into a fine powder by shaking in a Tissue II Lyser. Ball bearings were removed from vials and crushed tissue samples were sent to Cornell University Stable Isotope laboratory for d13C (carbon stable isotope) and d15N (nitrogen stable isotope) analysis. Stable isotope ratios are expressed in parts per thousand units using the standard delta (d) notation d13C and d15N. Data Set This data set consists of an Excel spreadsheet containing raw data of Nitrogen and Carbon Stable Isotope analysis from 6 sites in the Prydz Bay area of East Antarctica. It includes site distance and direction from wastewater discharge point. The file name code stable isotope analysis is; Project number_Season_Taxa_analysis type AAS_4177_12_13_Trematomus_Isotopes Project number : AAS_4177 Season : 2012/13 season Taxa: Trematomus Analysis type: Stable Isotope Metal analysis. Davis Station Laboratory Dorsolateral muscle tissue from the right side of each individual was removed, placed in a plastic zip lock bag and frozen at -20 degrees C for later analysis. Tissue processing 10g of frozen muscle tissue was sent to Advanced Analytical Australia for metal analysis of a suite of metals (Cd, Cr, Cu, Hg, Mn, Zn, Al, Ni, Pb). Data Set This data set consists of an Excel spreadsheet containing raw data of metal analysis (mg/kg) from 6 sites in the Prydz Bay area of East Antarctica. It includes site distance and direction from wastewater discharge point. The file name code stable isotope analysis is; Project number_Season_Taxa_analysis type AAS_4177_12_13_Trematomus_Metals Project number : AAS_4177 Season : 2012/13 season Taxa: Trematomus Analysis type: Metal analysis Histological analysis Davis Station Laboratory A small piece of a number of fish tissues (gill, liver, spleen, head kidney, gonad), were collected immediately after death of the fish to ensure no degradation of tissue and preserved in 10% seawater buffered formalin for later analysis Tissue processing Each piece of tissue was dehydrated in ascending grades of ethanol (30-100%), cleared in Histolene and embedded in paraffin wax. Tissue was sectioned using a HM 32 Micron microtome at 4 microns. Standard haematoxylin and eosin (H and E) stain was used to stain all tissue sections. Each section was examined blind (i.e. the examiner did not know the field location of the tissue samples) using a Zeiss AxioPlan microscope at 100-400 x magnification. Histological analysis is ongoing. Data Set This data set consists of a pdf file with images of normal and potential sub-lethal histological alterations. The file name code stable isotope analysis is; Project number_Season_Taxa_analysis type AAS_4177_12_13_Trematomus_Histology Project number : AAS_4177 Season : 2012/13 season Taxa: Trematomus Analysis type: Histopathology

Metadata record for data from ASAC Project 2388 See the link below for public details on this project. ---- Public Summary from Project ---- The HIMI Ecosystem Project is aimed at examining key predators based on Heard Island, including albatross, penguins and seals, and their interactions with prey, the ocean and benthic environment and commercial fisheries. Work will include examining the diet of the animals, tracking them to determine where they feed, and examining prey available in the feeding areas. Scientists from a variety of backgrounds including bird and mammal biologists, marine biologists, oceanographers and marine geologists will be involved. These data were collected on Aurora Australis voyage 4 2004 ('HIPPIES'). The data are stored in a Microsoft Access 97 file. The data includes sampling information for trawls using an IYGPT (International Young Gadoid Pelagic Trawl) Net and RMT 8 (8 square meter Rectangular Midwater Trawl) Nets. Sampling data, including location and time, are stored in the 'Hauls' table. Data from the analysis of the contents of each trawl, including species identifications, mass and counts of each taxon of mesopelagic fish and zooplankton are stored in the 'Bycatch' table. Weights, lengths and other biological data collected from individual mesopelagic fish are stored in the 'Fish Data' table. On opening the file the Haul form launches automatically giving access to the data. The fields in this dataset are: Latitude Longitude Species Date Haul Number Time Depth (m) Tow distance Wire out Tow Speed (knots) Headline height (m) Headline width (m) Gear Comments Fishing Ground Fishery Traps Line Hooks Magazines Fish length Otoliths Scale sample Fish Weight Stomach Gonads Stomach contents