These data were generated by Raffaella Tolotti (raffaella.tolotti@virgilio.it) thanks to a scholarship founded by the Italian P.N.R.A. ‘TYTAN Project (PdR 14_00119): ‘Totten Glacier dYnamics and Southern Ocean circulation impact on deposiTional processes since the mid-lAte CeNozoic’ (Principal Investigator Dr. Donda Federica, Dr. Caburlotto A. - OGS, Trieste) and University of Genova (DISTAV - Prof. Corradi Nicola). These data are based on samples collected during research cruise IN2017_V01 of the RV Investigator, co-chief scientists, Leanne Armand and Phil O’Brien and were collected to provide paleoceanographic and bio/ stratigraphic information on Aurora Basin Antarctic margin evolution. The IN2017-V01post-cruise report is available through open access via the e-document portal through the ANU library. https://openresearch-repository.anu.edu.au/handle/1885/142525 The document DOI: 10.4225/13/5acea64c48693 The preferred citation are: L.K. Armand, P.E. O’Brien and On-board Scientific Party. 2018. Interactions of the Totten Glacier with the Southern Ocean through multiple glacial cycles (IN2017-V01): Post-survey report, Research School of Earth Sciences, Australian National University: Canberra, http://dx.doi.org/10.4225/13/5acea64c48693 Donda F., Leitchenkov, Brancolini G., Romeo R., De Santis L., Escutia C., O'Brien P., Armand L., Caburlotto, A., Cotterle, D., 2020. The influence of Totten Glacier on the Late Cenozoic sedimentary record. Antarctic Science, 1 -3; http://doi:10.1017/S0954102020000188 O’Brien, P.E., Post, A.L., Edwards, S., Martin, T., Carburlotto, A., Donda, F., Leitchenkov, G., Romero, R., Duffy, M., Evangelinos, D., Holder, L., Leventer, A., López-Quirós, A., Opdyke, B.N., and Armand, L.K. in press. Continental slope and rise geomorphology seaward of the Totten Glacier, East Antarctica (112°E-122°E). Marine Geology. Samples for diatom analysis were collected on board ship immediately after core recovery. Sub-samples were sent, according to the Australian standard procedures, to the DISTAV sedimentological laboratory in Genoa (Italy) and prepared for the micro-paleontological analysis according to the laboratory’s protocol (imported and tested from Salamanca University lab.; Referring Prof. Bárcena). Smear-slides and the qualitative-quantitative analyses were performed every 20 cm. Previous onboard smear slides analyses on PC03 highlighted notable variations from the other piston cores, containing some older diatom species. Moreover this core exceptionally did not exhibit a clear cyclicity like the others. It was so assumed to target a condensed sedimentary sequence giving access to older sediments. The further, more in-depth diatom biostratigraphic and quantitative analyses were performed in accordance with the international stratigraphic guide (https://stratigraphy.org/guide/), with the pluri-decennial DSDP and IODP Antarctic diatom biostratigraphic reports and specific papers (see References). Sample preparation, diatom species identification and counting were those described in Schrader and Gersonde (1978), Barde (1981 - modified) and Bodén (1991). Diatom analysis was performed with an immersion 1000x LM Reichert Jung-Polyvar microscope (Wien). Whenever possible, almost 300 diatom valves were counted per slide following the counting methodology presented in Schrader and Gersonde (1978). When diatom concentration proved too low or too concentrated, slides with modified concentrations have been prepared to optimize counting and identification while at least one hundred fields-of-view per poor concentration slide have been analyzed. For samples that were too diatom-poor, the over-concentration of material on the slides resulted in limiting resolution and taxonomic identification of the rare and mostly fragmented valves. Where diatom occurrence was rare only major fragments (>50%) or entire valves were counted. The file (.xls) contains 2 sheets: Sheet: PC03 diatoms dataset. The absolute diatom valve concentration (ADA= Absolute Valves Abundance) was then calculated following Abrantes et al. (2005), Warnock and Scherer (2014) and ADA in Taylor, Silva and Riesselmann (2018), taking in account initial weights, concentration of the samples and microscope’s characteristics, as the number of valves per gram of dry sediment. Diatoms were identified to species level following Crosta et al. (2005), Armand et al. (2005), Cefarelli et al. (2010) for modern assemblages. Older diatom taxa were identified following Gersonde et Bárcena, 1998, Witkowski et al., 2014; Bohaty et al., 2011; Gombos, 1985; Gombos, 2007; Gersonde et al., 1990; Barron et al., 2004; Harwood et al., 2001; Harwood etal., 1992. Species were considered extinct when observed stratigraphically higher than extinction boundaries as identified by Cody et al. (2008) but the coexistence or the alternation in the stratigraphic sequence of taxa referring to different biostratigraphic age ranges were considered signs of reworking. Sheet: PC03 tephra dataset. During LM microscopic observations some volcanic glass shards were observed first in smear slides and then counted during the activities of microfossils count for diatoms. This allowed to obtain the number of glass shards/g. dry sed. useful to compare with diatom and sediment datasets. Core location: Station_core Longitude Latitude A006_PC03 115.043 -64.463 Depth: The core was taken at Site A006 that was chosen into an overbank deposit on the upper western side of a turbidite channel (Minang-a Canyon) (Fig. 39 – Armand et al., 2017; O’Brien et al., 2020). The setting is at 1862 m depth, shallower respect the other cores. A possible higher energy environment, with a lower sedimentation rate has been first supposed. Temporal coverage: Start date: 2017-01-14 - Stop date: 2018-11-30 References: Armand, L.K., X. Crosta, O. Romero, J. J. Pichon (2005). The biogeography of major diatom taxa in Southern Ocean sediments: 1. Sea ice related species, Paleogeography, Paleoclimatology, Paleoecology, 223, 93-126. Cefarelli, A.O., M. E. Ferrario, G. O. Almandoz, A. G. Atencio, R. Akselman, M. Vernet (2010). Diversity of the diatom genus Fragilariopsis in the Argentine Sea and Antarctic waters: morphology, distribution and abundance, Polar Biology, 33(2), 1463-1484. Cody, R., R. H. Levy, D. M. Harwood, P. M. Sadler (2008). Thinking outside the zone: High-resolution quantitative diatom biochronology for the Antarctic Neogene, Palaeogeography, Palaeoclimatology, Palaeoecology, 260, 92-121; doi:10.1016/j.palaeo.2007.08.020 Crosta, X., O. Romero, L. K. Armand, J. Pichon (2005). The biogeography of major diatom taxa in Southern Ocean sediments: 2. Open ocean related species, Palaeogeography, Palaeoclimatology, Palaeoecology, 223, 66-92. Rebesco, M., E. Domack, F. Zgur, C. Lavoie, A. Leventer, S. Brachfeld, V. Willmott, G. Halverson, M. Truffer, T. Scambos, J. Smith, E. Pettit (2014). Boundary condition of grounding lines prior to collapse, Larsen-B Ice Shelf, Antarctica, Science, 345, 1354-1358. Warnock, J. P., R. P. Scherer (2014). A revised method for determining the absolute abundance of diatoms, J. Paleolimnol.; doi:10.1007/s10933-014-9808-0 Witkowski, J., Bohaty, S.M., McCartney, K., Harwood, D.M., (2012) . Enhanced siliceous plankton productivity in response to middle Eocene warming at Southern Ocean ODP Sites 748 and 749 Palaeogeog., Palaeoclimat., Palaeoecol., 326–328, 78–94; doi:10.1016/j.palaeo.2012.02.006 Witkowski, J., Bohaty, S.M., Edgar, K.M., Harwood, D.M., (2014). Rapid fluctuations in mid-latitude siliceous plankton production during the Middle Eocene Climatic Optimum (ODP Site 1051, Western North Atlantic). Mar. Micropal., 106, 110–129. http://dx.doi.org/10.1016/j.marmicro.2014.01.001 Raffaella Tolotti unpublished data

Project 565: The database provides a list of species of ciliates and testate amoebae (Protozoa: Ciliophora; Testacea) recorded in various edaphic habitats, e.g., mineral soils (fellfield), ornithogenic soils, terrestrial mosses, from ice-free coastal areas and inshore islands in the area of Casey Station, Wilkes Land, coastal continental Antarctica. 26 ciliate (9 first records for continental Antarctica, 1 undescribed) and 5 testacean species (3 new records) were found. Sea ice study (Weddell Sea): The ciliate biodivesity was studied in several types of sea ice (mainly young pancake ice) from the Weddell Sea, Antarctica, in the austral autumn 1992 (March-May) during the cruise ANT X/3 of RV Polarstern. 49 ciliate species were predominantly found in sea ice and 6 spp. in the pelagial; 20 of these were new to science. A word document containing a list of species that were recorded as part of the project is available for download from the provided URL. These data have also been incorporated into the biodiversity database.

The biodiversity database is planned to be a reference on Antarctic and subantarctic flora and fauna collated by the Regional Sensitivity to Climate Change (RiSCC) group and developed by the Australian Antarctic Data Centre. Searches are available in the following areas: Taxonomy Protection and convention measures (protected species) Observations Scientific Bibliographies

Antarctic sediments and sea-ice are important regulators in global biogeochemical and atmospheric cycles. These ecosystems contain a diverse range of bacteria whose biogeochemical roles remains largely unknown and which inhabit what are continually low temperature habitats. An integrated molecular and chemical approach will be used to investigate the coupling of microbial biogeochemical processes with community structure and cold adaptation within coastal Antarctic marine sediments and within sea-ice. Overall the project expects to make an important contribution to our understanding of biological processes within low temperature habitats. DATA SET ORGANISATION: The dataset is organised on the basis of publication and is organised on the basis of the following sections: 1. SEDIMENT SAMPLES and ISOLATES Samples collected are described in terms of location, type and where data were obtained chemical features. The designation, source, media used for cultivation and isolation and availability of sediment and other related isolates are provided. Samples included are from the following locations: Clear Lake, Pendant Lake, Scale Lake, Ace Lake, Burton Lake, Ekho Lake, Organic Lake, Deep lake and Taynaya Bay (Burke Basin), Vestfold Hills region; and the Mertz Glacier Polynya region. 2. BIOMASS and ENZYME ACTIVITY DATA Biomass, numbers and extracellular enzyme activity data are provided for Bacteria and Archaea populations from Mertz Glacier Polynya shelf sediments. 3. FATTY ACID and TETRAETHER LIPID DATA Phospholipid and tetraether lipid data are provided for Mertz Glacier Polynya shelf sediments. Whole cell fatty acid data are provided for various bacterial isolates described officially as new genera or species. 4. RNA HYBRIDISATION DATA RNA hybridisation data for Mertz Glacier Polynya sediment samples is provided, including data for oligonucleotide probes specifc for total Bacteria, Archaea, the Desulfosarcina group (class Deltaproteobacteria, sulfate reducing bacterial clade), phylum Planctomycetes, phylum Bacteroidetes (Cytophaga-Flavobacterium-Bacteroides), class Gammaproteobacteria, sulfur-oxidizing and related bacteria (a subset of class Gammaproteobacteria) and Eukaryota. 5. PHYLOGENETIC DATA 16S rRNA gene sequence data are indicated including aligned datasets for three clone libraries derived from the Mertz Glacier Polynya including GenBank accession numbers. Sequence accession numbers are provided for Vestfold Hills lake sediment samples. In addition GenBank numbers are provided for denaturing gradient gel electrophoresis band sequence data from Mertz Glacier Polynya shelf sediment. Other forms of this DGGE data (banding profile analysis) are available in reference Bowman et al. 2003 (AAD ref 10971).