Full title: Diatom and associated data for a manipulative field experiment examining the effects of heavy metal and petroleum hydrocarbon contamination on benthic diatom communities in the Windmill Islands, Antarctica. A manipulative field experiment was performed to assess the effects of heavy metals and petroleum hydrocarbons on benthic diatom communities in the Windmill Islands. Three treatments were used (control, metal contaminated, and petroleum hydrocarbon contaminated), with replicates of each treatment deployed at three locations (Sparkes Bay, Brown Bay and O'Brien Bay). The datasets associated with this experiment include the concentrations of metals and hydrocarbons within samples, as well as diatom data (raw counts, and the relative abundance of benthic species). This work was completed as part of ASAC project 1130 (ASAC_1130) and project 2201 (ASAC_2201). Public summary from project 1130: Algal mats grow on sea floor in most shallow marine environments. They are thought to contribute more than half of the total primary production in many of these areas, making them a critical food source for invertebrates and some fish. We will establish how important they are in Antarctic marine environments and determine the effects of local sewerage and tip site pollution. We will also investigate the impact on the algal mats of the additional UV radiation which results from the ozone hole. Public summary from project 2201: As a signatory to the Protocol on Environmental Protection to the Antarctic Treaty Australia is committed to comprehensive protection of the Antarctic environment. This protocol requires that activities in the Antarctic shall be planned and conducted on the basis of information sufficient to make prior assessments of, and informed judgements about, their possible impacts on the Antarctic environment. Most of our activities in the Antarctic occur along the narrow fringe of ice-free rock adjacent to the sea and many of our activities have the potential to cause environmental harm to marine life. The Antarctic seas support the most complex and biologically diverse plant and animal communities of the region. However, very little is known about them and there is certainly not sufficient known to make informed judgements about possible environmental impacts. The animals and plants of the sea-bed are widely accepted as being the most appropriate part of the marine ecosystem for indicating disturbance caused by local sources. Attached sea-bed organisms have a fixed spatial relationship with a given place so they must either endure conditions or die. Once lost from a site recolonisation takes some time, as a consequence the structure of sea-bed communities reflect not only present conditions but they can also integrate conditions in the past. In contrast, fish and planktonic organisms can move freely so their site of capture does not indicate a long residence time at that location. Because sea-bed communities are particularly diverse they contain species with widely differing life strategies, as a result different species can have very different levels of tolerance to stress; this leads to a range of subtle changes in community structure as a response to gradually increasing disturbance, rather than an all or nothing response. This project will examine sea-bed communities near our stations to determine how seriously they are affected by human activities. This information will be used to set priorities for improving operational procedures to reduce the risk of further environmental damage. The fields in this dataset are: Species Site Abundance Treatment Type Antimony Arsenic Cadmium Chromium Copper Iron Lead Manganese Mercury Nickel Silver Tin Zinc Special Antarctic Blend Lube

These results are for the 4 hour extraction of HCl. See also the metadata records for the 0.5 hour extraction of HCl, and the time trial data for 1 M HCl extractions. A regional survey of potential contaminants in marine or estuarine sediments is often one of the first steps in a post-disturbance environmental impact assessment. Of the many different chemical extraction or digestion procedures that have been proposed to quantify metal contamination, partial acid extractions are probably the best overall compromise between selectivity, sensitivity, precision, cost and expediency. The extent to which measured metal concentrations relate to the anthropogenic fraction that is bioavailable is contentious, but is one of the desired outcomes of an assessment or prediction of biological impact. As part of a regional survey of metal contamination associated with Australia's past waste management activities in Antarctica, we wanted to identify an acid type and extraction protocol that would allow a reasonable definition of the anthropogenic bioavailable fraction for a large number of samples. From a kinetic study of the 1 M HCl extraction of two certified Certified Reference Materials (MESS-2 and PACS-2) and two Antarctic marine sediments, we concluded that a 4 hour extraction time allows the equilibrium dissolution of relatively labile metal contaminants, but does not favour the extraction of natural geogenic metals. In a regional survey of 88 marine samples from the Casey Station area of East Antarctica, the 4 h extraction procedure correlated best with biological data, and most clearly identified those sediments thought to be contaminated by runoff from abandoned waste disposal sites. Most importantly the 4 hour extraction provided better definition of the low to moderately contaminated locations by picking up small differences in anthropogenic metal concentrations. For the purposes of inter-regional comparison, we recommend a 4 hour 1 M HCl acid extraction as a standard method for assessing metal contamination in Antarctica. The fields in this dataset are Location Site Replicate Antimony Arsenic Cadmium Chromium Copper Iron Lead Manganese Nickel Silver Tin Zinc

These results are for the time trial extraction of HCl. See also the metadata records for the 0.5 and 4 hour extractions of HCl. A regional survey of potential contaminants in marine or estuarine sediments is often one of the first steps in a post-disturbance environmental impact assessment. Of the many different chemical extraction or digestion procedures that have been proposed to quantify metal contamination, partial acid extractions are probably the best overall compromise between selectivity, sensitivity, precision, cost and expediency. The extent to which measured metal concentrations relate to the anthropogenic fraction that is bioavailable is contentious, but is one of the desired outcomes of an assessment or prediction of biological impact. As part of a regional survey of metal contamination associated with Australia's past waste management activities in Antarctica, we wanted to identify an acid type and extraction protocol that would allow a reasonable definition of the anthropogenic bioavailable fraction for a large number of samples. From a kinetic study of the 1 M HCl extraction of two certified Certified Reference Materials (MESS-2 and PACS-2) and two Antarctic marine sediments, we concluded that a 4 hour extraction time allows the equilibrium dissolution of relatively labile metal contaminants, but does not favour the extraction of natural geogenic metals. In a regional survey of 88 marine samples from the Casey Station area of East Antarctica, the 4 h extraction procedure correlated best with biological data, and most clearly identified those sediments thought to be contaminated by runoff from abandoned waste disposal sites. Most importantly the 4 hour extraction provided better definition of the low to moderately contaminated locations by picking up small differences in anthropogenic metal concentrations. For the purposes of inter-regional comparison, we recommend a 4 hour 1 M HCl acid extraction as a standard method for assessing metal contamination in Antarctica. The fields in this dataset are Concentration Extraction Time Antimony Arsenic Cadmium Chromium Copper Iron Lead Manganese Nickel Silver Tin Zinc

These results are for the 0.5 hour extraction of HCl. See also the metadata records for the 4 hour extraction of HCl, and the time trial data for 1 M HCl extractions. A regional survey of potential contaminants in marine or estuarine sediments is often one of the first steps in a post-disturbance environmental impact assessment. Of the many different chemical extraction or digestion procedures that have been proposed to quantify metal contamination, partial acid extractions are probably the best overall compromise between selectivity, sensitivity, precision, cost and expediency. The extent to which measured metal concentrations relate to the anthropogenic fraction that is bioavailable is contentious, but is one of the desired outcomes of an assessment or prediction of biological impact. As part of a regional survey of metal contamination associated with Australia's past waste management activities in Antarctica, we wanted to identify an acid type and extraction protocol that would allow a reasonable definition of the anthropogenic bioavailable fraction for a large number of samples. From a kinetic study of the 1 M HCl extraction of two certified Certified Reference Materials (MESS-2 and PACS-2) and two Antarctic marine sediments, we concluded that a 4 hour extraction time allows the equilibrium dissolution of relatively labile metal contaminants, but does not favour the extraction of natural geogenic metals. In a regional survey of 88 marine samples from the Casey Station area of East Antarctica, the 4 h extraction procedure correlated best with biological data, and most clearly identified those sediments thought to be contaminated by runoff from abandoned waste disposal sites. Most importantly the 4 hour extraction provided better definition of the low to moderately contaminated locations by picking up small differences in anthropogenic metal concentrations. For the purposes of inter-regional comparison, we recommend a 4 hour 1 M HCl acid extraction as a standard method for assessing metal contamination in Antarctica. The fields in this dataset are Location Site Replicate Antimony Arsenic Cadmium Chromium Copper Iron Lead Manganese Nickel Silver Tin Zinc

Sediment samples were collected from nine points along 3 parallel transects within the contaminated Brown Bay. The diatom spreadsheet (diatom_data) contains both initial diatom counts and the relative abundance of benthic species. The abbreviation used to identify species are explained in the separate file called sp_list. Metal, Total Purgeable Hydrocarbons (TPH), and grain-size data are all presented as separate files. This work was completed as part of ASAC project 1130 (ASAC_1130) and project 2201 (ASAC_2201). Public summary from project 1130: Algal mats grow on sea floor in most shallow marine environments. They are thought to contribute more than half of the total primary production in many of these areas, making them a critical food source for invertebrates and some fish. We will establish how important they are in Antarctic marine environments and determine the effects of local sewerage and tip-site pollution. We will also investigate the impact on the algal mats of the additional UV radiation which results from the ozone hole. Public summary from project 2201: As a signatory to the Protocol on Environmental Protection to the Antarctic Treaty Australia is committed to comprehensive protection of the Antarctic environment. This protocol requires that activities in the Antarctic shall be planned and conducted on the basis of information sufficient to make prior assessments of, and informed judgements about, their possible impacts on the Antarctic environment. Most of our activities in the Antarctic occur along the narrow fringe of ice-free rock adjacent to the sea and many of our activities have the potential to cause environmental harm to marine life. The Antarctic seas support the most complex and biologically diverse plant and animal communities of the region. However, very little is known about them and there is certainly not sufficient known to make informed judgements about possible environmental impacts The animals and plants of the sea-bed are widely accepted as being the most appropriate part of the marine ecosystem for indicating disturbance caused by local sources. Attached sea-bed organisms have a fixed spatial relationship with a given place so they must either endure conditions or die. Once lost from a site recolonisation takes some time, as a consequence the structure of sea-bed communities reflect not only present conditions but they can also integrate conditions in the past. In contrast, fish and planktonic organisms can move freely so their site of capture does not indicate a long residence time at that location. Because sea-bed communities are particularly diverse they contain species with widely differing life strategies, as a result different species can have very different levels of tolerance to stress; this leads to a range of subtle changes in community structure as a response to gradually increasing disturbance, rather than an all or nothing response. This project will examine sea-bed communities near our stations to determine how seriously they are affected by human activities. This information will be used to set priorities for improving operational procedures to reduce the risk of further environmental damage. The fields in this dataset are: bbg_lat spreadsheet Site Latitude Longitude Easting Northing Diatoms spreadsheet Species Site Abundance Transect Metals Spreadsheet Sample Antimony Arsenic Cadmium Chromium Copper Iron Lead Manganese Mercury Nickel Silver Tin Zinc Total Organic Carbon Easting Northing TPH Spreadsheet Site Total Purgeable Hydrocarbons Fraction of Purgeable Hydrocarbons

Blood samples from 35 seals have been collected for serum biochemistry analysis. Scats from 20 animals have been collected for parasitology. Estimated weights and morphometric measurements from 35 animals have been collected. The data for this project are presented in a number of excel worksheets. In addition, a word document is also included in the download file which fully explains each spreadsheet. A precis of that word document is copied below. Haematology Data from haematological analysis performed on fresh whole blood collected from leopard seals between 27.12.1999-22.01.2002 and Weddell seals between 02.01.2001-21.01.2002 off Davis Station in the Austral summer seasons of 1999/2000, 2000/2001 and 2001/2002. Serum protein electrophoresis (SPE) Data from SPE analysis performed on serum (stored at -80 degrees C) collected from leopard seals between 27.12.1999-22.01.2002 and Weddell seals between 02.01.2001-21.01.2002 off Davis Station in the Austral summer seasons of 1999/2000, 2000/2001 and 2001/2002. Serum Biochemistry Data from biochemistry analysis performed on serum (stored at -80 degrees C) collected from leopard seals between 27.12.1999-22.01.2002 and Weddell seals between 02.01.2001-21.01.2002 off Davis Station in the Austral summer seasons of 1999/2000, 2000/2001 and 2001/2002. Trace element and heavy metal analysis Data from trace element and heavy metal analysis performed on serum (-80 degrees C), fur, frozen (-20 degrees C) and formalin (10%) fixed tissues, plasma (-80 degrees C), EDTA plasma (-80 degrees C), washed red blood cells (-80 degrees C) and urine (-20 degrees C) collected from leopard seals between 27.12.1999-17.02.2002 and Weddell seals between 02.01.2001-21.01.2002 off Davis Station in the Austral summer seasons of 1999/2000, 2000/2001 and 2001/2002 using inductively coupled plasma mass spectroscopy. The spreadsheet is organised into six worksheets: 1.Serum (micro g/L) 2.Fur (micro g/g dry weight) 3.Frozen tissues (micro g/g dry weight) 4.Plasma and RBC (red blood cells) (micro g/L) 5.Urine (micro g/L) 6.Formalin tissues (micro g/g dry weight) Faecal Parasites Data from faecal flotation in saturated salt solution performed on fresh and frozen (- 20 degrees C) scats collected from leopard seals between 23.11.1999-17.02.2002 and Weddell seals between 06.12.2000-16.01.2002 off Davis Station in the Austral summer seasons of 1999/2000, 2000/2001 and 2001/2002. The fields in this dataset are: Glucose Urea Creatinine Fibrinogen Protein Albumin Globulin Bilirubin ALP AST ALT Creatinine Kinase Cholesterol Calcium Phosphate Sodium Potassium Chloride Bicarbonate Anion Gap Amylase Lipase Date Faeces Cestode eggs Ascarid Eggs PCV WCC Neutrophil Eosinophil Basophil Lymphocyte Monocyte Band Neutrophil Serum Magnesium Aluminium Vandium Chromium Manganese Iron Cobalt Nickel Copper Zinc Arsenic Selenium Cadmium Mercury Lead Bismuth Date