This is a scanned copy of the report written by Simon Townsend on work undertaken at Davis Station during the wintering year of 1989. The report covers the following topics: - Tierny Drainage System - The hypersaline density current hypothesis tested - Ellis Fjord temperature and salinity data - Ellis Fjord long-term instrument deployment - Water tracer experiment - Organic Lake - Ellis Fjord in-situ chlorophyll-a profiles - Appendices: Platypus notes, Platypus software, Seabird instrument notes, assessment of Chelsea suspended solids meter, winches for biological use, advise under-ice instrument deployment.

Colonisation of Lake Fletcher, a hypersaline, meromictic lake in the Vestfold Hills, Antarctica, by the calanoid copepod Drepanopus bispinosus, the cyclopoid copepod Oncea curvata and an undescribed cydippid ctenophore is discussed. In 1978, salinity direstly under the ice was 66 ppt and repeated net hauls found no zooplankton. In 1983, adults of D. bispinosus were found, and in 1984, a reproductively active population of this species. Surface water salinity in 1984 was 56 ppt. During winter 1986, surface salinity was 54 ppt and three zooplankton species (D. bispinosus, O curvata and an undescribed cydippid ctenophore) had established populations in the lake. In 1986/87, high tides caused nearby Taynaya Bay to flood into the lake, and three further species (the calanoid, Paralabidocera antarctica, and two harpacticoids, Harpacticus furcatus and Idomene sp.) were found in the lake. It appears that periodic flooding after 1978 caused a salinity decrease in the lake from 66 to 54 ppt, and this enabled some invertebrate species to maintain year-round populations, whereas others require marine incursions to re-establish summer only populations. The fields in this dataset are: Date Salinity Record Species

The sedimentological, chemical and isotopic characteristics of sediment cores from three slightly saline to hypersaline lakes (Highway, Ace and Organic Lakes) and two marine inlets (Ellis Fjord and Taynaya Bay) in the Vestfold Hills, Antarctica have been examined. Sections of the cores deposited in marine environments are characterised by uniform, regularly laminated, fine grained, organic-rich sediments, with uniform organic delta 13C values (-18.0 to 19.4 ppt vs. PDB) and sulfur contents. In contrast, sediments deposited in lacustrine environments are extremely heterogeneous, varying from finely laminated mat-like sequences to poorly sorted clastic-rich sediments. Authigenic monohydrocalcite and aragonite occur in some lake sediments. The delta 13C values of organic matter in the lacustrine sediments exhibit an extremely wide range (-10.5 to -25.3 ppt) that can be related to variations in physico-chemical conditions in the lake waters. Strongly negative organic-delta 13C values coupledwith high sulfur contents are indicative of an anoxic zone in the overlying lake waters, whereas less negative organic-delta 13C values coupled with low sulfur contents are indicative of well-mixed oxic conditions. Particularly high organic-delta 13C values result during high levels of microbial activity in the lakes, due to high rates of photosynthetic CO2 fixation. The large shifts in organic-delta 13C are not necessarily accompanied by any change in macroscopic sedimentological characteristics, illustrating the utility if isotopic investigations in these environments. The delta 13C composition of authigenic carbonate in hypersaline Organic Lake sediments provides a record of changes in palaeoproductivity, while the delta 18O of the carbonate provides information on rates of meltwater input and evaporation in the lake. 14C-dating suggests that Highway Lake was isolated from the sea by isostatic uplift at least 4600 years before present (BP) whereas Organic Lake was isolated at approximately 2700 years BP. Apparent emergence rates calculated from the 14C ages range from 1.0 to 2.1 mm per year. The 'reservoir effect' in the lacustrine and marine environments is variable, but probably does not exceed ~ 1000 years in any of the lakes examined.

From the abstracts of some of the referenced papers: The relationship between surface sediment diatom assemblages and measured limnological variables in 33 coastal Antarctic lakes was examined by constructing a diatom-water chemistry dataset. Canonical correspondence analysis revealed that salinity and silicate each explain significant amounts of variation in the distribution and abundance of the surface sediment diatom taxa. Salinity has the strongest influence, revealing its value for limnological inference models in this coastal Antarctic region. A comprehensive diatom stratigraphy is used to calculate a palaeosalinity history for an Antarctic lake via an established diatom-salinity transfer function for the Vestfold Hills, Antarctica. A sediment core taken from Ace Lake in 1995 shows three distinct changes in diatom assemblage constituents: initial benthic hyposaline - freshwater taxa are replaced by marine planktonic and sea-ice taxa with these taxa in turn replaced by the benthic hypersaline taxa dominant in the lake today. These changes in assemblage composition enable the lakewater salininty of each stage to be determined, and the Holocene evolution of the lake to be refined. Deglaciation of the Vestfold Hills at the beginning of the Holocene exposed Ace Lake basin; following this, fresh lacustrine diatoms were deposited from ~11 380 to ~8110 corrected 14C yrBP. Relative sea-level rise after this time led to the progressive marine inundation of the lake and the deposition of marine diatom taxa. Marine taxa were dominant in the sediment for more than 6000 years. Isostatic rebound and stabilisation of the sea-level isolated Ace Lake and at ~1480 corrected 14C yrBP saline lacustrine diatoms became the dominant taxa, indicative of the concentration of dissolved salts through evaporation after isolation.

Antarctic sediments and sea-ice are important regulators in global biogeochemical and atmospheric cycles. These ecosystems contain a diverse range of bacteria whose biogeochemical roles remains largely unknown and which inhabit what are continually low temperature habitats. An integrated molecular and chemical approach will be used to investigate the coupling of microbial biogeochemical processes with community structure and cold adaptation within coastal Antarctic marine sediments and within sea-ice. Overall the project expects to make an important contribution to our understanding of biological processes within low temperature habitats. DATA SET ORGANISATION: The dataset is organised on the basis of publication and is organised on the basis of the following sections: 1. SEDIMENT SAMPLES and ISOLATES Samples collected are described in terms of location, type and where data were obtained chemical features. The designation, source, media used for cultivation and isolation and availability of sediment and other related isolates are provided. Samples included are from the following locations: Clear Lake, Pendant Lake, Scale Lake, Ace Lake, Burton Lake, Ekho Lake, Organic Lake, Deep lake and Taynaya Bay (Burke Basin), Vestfold Hills region; and the Mertz Glacier Polynya region. 2. BIOMASS and ENZYME ACTIVITY DATA Biomass, numbers and extracellular enzyme activity data are provided for Bacteria and Archaea populations from Mertz Glacier Polynya shelf sediments. 3. FATTY ACID and TETRAETHER LIPID DATA Phospholipid and tetraether lipid data are provided for Mertz Glacier Polynya shelf sediments. Whole cell fatty acid data are provided for various bacterial isolates described officially as new genera or species. 4. RNA HYBRIDISATION DATA RNA hybridisation data for Mertz Glacier Polynya sediment samples is provided, including data for oligonucleotide probes specifc for total Bacteria, Archaea, the Desulfosarcina group (class Deltaproteobacteria, sulfate reducing bacterial clade), phylum Planctomycetes, phylum Bacteroidetes (Cytophaga-Flavobacterium-Bacteroides), class Gammaproteobacteria, sulfur-oxidizing and related bacteria (a subset of class Gammaproteobacteria) and Eukaryota. 5. PHYLOGENETIC DATA 16S rRNA gene sequence data are indicated including aligned datasets for three clone libraries derived from the Mertz Glacier Polynya including GenBank accession numbers. Sequence accession numbers are provided for Vestfold Hills lake sediment samples. In addition GenBank numbers are provided for denaturing gradient gel electrophoresis band sequence data from Mertz Glacier Polynya shelf sediment. Other forms of this DGGE data (banding profile analysis) are available in reference Bowman et al. 2003 (AAD ref 10971).