Ozone depletion over Antarctica increases UVB irradiances reaching the Earth's surface in the region. Marine microbes, that support the Antarctic food web and play an integral part in carbon cycling, are damaged by UVB. This research determines Antarctic UV climate, biological responses to UV from the molecular to community level, and combines these elements to predict UV-induced changes in Antarctic marine microbiology. A season of field work was undertaken over November and December 1994 based from Davis Station with the intention of making field measurements of ultraviolet radiation in the fast ice environment, as well as some of the lakes in the Vestfold Hills. Instrumentation The instrument for the measurements was a Macam spectral radiometer, owned by Geography and Environmental Studies, University of Tasmania. Field personnel were Dr Kelvin Michael (IASOS) and Mr Michael Wall (Honours student, Geography and Environmental Studies, UTas). The radiometer was equipped with a 25-metre quartz light pipe, with a cosine sensor attachment at the end. To make a measurement of ultraviolet irradiance, the sensor would be oriented so that its sensing surface was horizontal, and it would collect light which was then transmitted along the light pipe to the radiometer - a suitcase-sized unit which ran on battery power in the field. The radiometer was encased in a wooden box lined with polystyrene foam to provide protection from the elements and heat insulation. The radiometer was controlled via a laptop PC and the data were stored on the hard disk of the PC. Measurements Measurements of the attenuation of ultraviolet and visible radiation as a function of wavelength in water were made at the ice edge and lake measurement sites. At the ice edge, the light pipe was spooled over a wheel and lowered to preset depths (typically 1,2,4,8,16 and 32 m below the water surface). On a lake, a 25-cm augur hole was drilled, and the light pipe was lowered by hand to various depths, the exact depths chosen depended on the depth of the lake. Where the lake ice conditions permitted, a frame was lowered through the hole and used to lever the light pipe against the underside of the ice and a measurement of the ultraviolet and visible transmission of the sea ice was collected. In all cases, measurements of the ultraviolet and visible surface irradiance were collected before and/or after the sub-surface measurements. When the sky conditions were sufficiently clear, the direct and diffuse components of the ultraviolet and visible irradiance values were estimated, via the use of a shading apparatus. This would ensure that the radiometer would measure the diffuse component of the radiation field, allowing the direct component to be estimated by subtraction of the diffuse from the global (unshaded) measurement. On some occasions, the upwelling irradiance from the snow or ice surface was also measured, providing information on the spectral albedo of the surface. At each measurement, spectral irradiance values were generally collected for two spectral ranges: UV-B (280 - 400 nm, in 1-nm steps) and visible (400 - 700 nm, in 5-nm steps). In some cases, the wavelength boundaries were different - eg 280 - 350 nm for the UV-B, or 550 - 680 nm in the visible (corresponding to channel 1 of the NOAA AVHRR sensor). The data were stored by the PC as raw data files. The names of these files are automatically defined from the time on the logging PC as 'hhmmss.dti'. Note that the PC was operating on Australian Eastern Summer Time, 4 hours ahead of DLT. These data files were later read into Excel spreadsheets for manipulation. See the linked report for further information. The measurements are all in units of watts per metre squared per nanometre (Wm^-2 nm_-1) The heading UV-B refers to the fact that the data are collected in the ultraviolet part of the spectrum (280 - 400 nm) The heading AVHRR refers to the fact that the data are collected in the visible part of the spectrum (400 - 700 nm) The fields in this dataset are: UV Radiation Wavelength Depth AVHRR

Increased ultraviolet radiation (UV-A and/or UV-B) may impact on the establishment and structure of a shallow water benthic invertebrate assemblages. A global experiment in more than 8 countries, using an identical methodology (transparent UV filters) will add significantly to our understanding of the impacts of anthropogenically induced global change on natural systems. To appraise the effects of increased UVR on shallow marine benthic assemblages, five experimental rafts were deployed in protected bays west of Shirley Island near Casey Station, Antarctica (66.16oS 110.30oE). Each raft consisted of eight experimental units, each of which contained a colonization panel (ceramic tile) positioned horizontally and submerged 4-6 cm underwater. Irradiation treatments were randomly assigned to each unit with the use of UV cut-off filters. The treatments were as follows: No UVR (transmits photosynthetically active radiation or PAR, 400-700nm), No UVB (transmits PAR + UVA, 320-700nm), Perspex (full spectrum, 280-700nm, procedural control), or No filter (full spectrum, treatment control). In addition there were three levels of consumer treatments: With consumption (container sides removed), without consumption (container sides perforated with 4 mm holes), and a control (3 sides perforated, 1 side removed). After seven weeks tiles were removed to the laboratory for examination. All tiles were dominated by diatoms and no sessile invertebrates were apparent. The first trial has been completed, but several other panels are still in place. A conference will be held in early 2002 between participating countries to discuss results and findings. The 2001\2002 summer season consisted of experimental designs divided up into three separate projects. The aims were all to provide a corrollary to the previous seasons data. Project 1 consisted of the extraction and redeployment of settlement depth arrays situated in Geoffrey's Bay and Hollin Island Channel. Due to prevailing weather conditions resulting in limited boating hours and diving program, only one array was retrieved. On inspection of the array it was decided to deploy further replicates to gain a better temporal understanding of the communities. Projects 2 and 3 consisted of a similar experimental design, however monitoring the shallower depths of settlement (depths of 1m and 2m below sea level) for a period of one month. Project 2 consisted of arrays with two depths and 2 panels per depth, triple replicated, under the icesheet in O'Brien Bay and Shirley Channel, with a substrate depth of 20m. Diatom samples are to be analyzed in Australia. Project 3 was of a similar design to project 2 though it was measuring recruitment in shallow open water. The two sites consisted of Noonan Cove and Geoffrey's Bay at substrate depths of 5m. These tiles are also to be analyzed on return to Australia. There were 5 rafts used in this study - they are listed as R1 to R5 there were two factors in the design -(i) predator access: Caged (C) Half caged (H) and Open (O) and ii) UV exposure: Perspex (P), Macrolon (M), No filter (N) and Film + perspex (F). A list of the diatoms found on the settlement panels is provided at the URL below. The fields in this dataset are: Species Sample