This spreadsheet contains species lists and counts from four sediment trap records. The sediment traps were deployed for ~1 year north and south of the Chatham Rise, New Zealand, between 1996 and 1997. Sheets 1a and 1b refer to North Chatham Rise (NCR). 1a = the 300m trap. 1b = the 1000m trap. Sheets 2a and 2b are for the South Chatham Rise traps (SCR). 2a= 300m, 2b= 1000m. Counting was undertaken on 1/16th splits. Material was cleaned of organics before diatom counting under light microscopy. Coccolith counting on uncleaned material was only undertaken at the 300m traps. Radiolarians and silicoflagellates were counted but not identified. Diatoms and coccoliths were counted along non-overlapping transects until 300 specimens had been counted per sample, or until 10 transects had been made. This dataset includes counts of diatom, coccolithophores, radiolarians and silicoflagellates for four sediment trap records- North Chatham Rise (NCR) and South Chatham Rise (SCR) at two trap depths each (300 m and 1000 m). It is intended as supplementary material to Wilks et al. 2018 (submitted) "Diatom and coccolithophore assemblages from archival sediment trap samples of the Subtropical and Subantarctic Southwest Pacific." Numbers are raw count per sample cup. Authorities are given. Coordinates of traps given in degrees, minutes and seconds.

From the abstract of the referenced paper: Spring phytoplankton communities in the water column of Ellis Fjord are characterised by diatoms originating from the bottom sea-ice strand community. Upon ice break-out in early summer, these are replaced by blooms of the phytoflagellates, Phaeocystis puchetii, Cryptomonas cryophila, Pyramimonas gelidicola, silicoflagellates and dinoflagellates. The narrow entrance of the fjord and the development of summer stratification is probably limiting the availability of nutrients and containing the magnitude of the small bloom (maximum 2.8 million cells per litre).

From the abstract of some of the papers: It has been suggested that increased springtime UVB radiation caused by stratospheric ozone depletion is likely to reduce primary production and induce changes in the species composition of Antarctic marine phytoplankton. Experiments conducted at Arthur Harbour in the Antarctic Peninsula revealed a reduction in primary productivity at both ambient and increased levels of UVB. Laboratory studies have shown that most species in culture are sensitive to high UVB levels, although the level at which either growth or photosynthesis is inhibited is variable. Stratospheric ozone depletion, with resultant increased springtime UVB irradiance, has been occurring with increasing severity since the late 1970's. Thus the phytoplankton community has already experienced about 20 years' exposure to increasing levels of UVB radiation. Here we present analyses of diatom assemblages from high-resolution stratigraphic sequences from anoxic basins in fjords of the Vestfold HIlls, Antarctica. We find that compositional changes in the diatom component of the phytoplankton community over the past 20 years cannot be distinguished from long-term natural variability, although there is some indication of a decline in the production of some sea-ice diatoms. We anticipate that our results are applicable to other Antarctic coastal regions, where thick ice cover and the timing of the phytoplankton bloom protect the phytoplankton from the effects of increased UVB radiation. Growth rate, survival, and stimulation of the production of UV-B (280 to 320 nm) absorbing compounds were investigated in cultures of five commonly occurring Antarctic marine diatoms exposed to a range of UV-B irradiances. Experimental UV-B exposures ranged from 20 to 650% of the measured peak surface irradiance at an Antarctic coastal site (0.533 J per square metre per second). The five diatom species (Nitzschia lecointei, Proboscia alata, P. inermis, Thalassiosira tumida and Stellarima microtrias) appear capable of surviving two to four times this irradiance. In contrast to Phaeocystis cf. pouchetti, another major component of the Antarctic phytoplankton, the concentrations of pigments with discrete UV absorption peaks in diatoms were low and did not change significantly under increasing UV-B irradiance. Absorbance of UV-B by cells from which pigments had been extracted commonly exceeded that of the pigments themselves. Most of this absorbance was due to oxidisable cell contents, with the frustule providing the remainder. Survival of diatoms did not correlate with absorption by either pigments, frustules or oxidisable cell contents, indicating that their survival under elevated UV-B irradiances results from processes other than screening mechanisms. Springtime UV-B levels have been increasing in Antarctic marine ecosystems since the 1970's. Effects on natural phytoplankton and sea-ice algal communities, however, remain unresolved. At the Marginal Ice Edge Zone, enhanced springtime UV-B levels coincide with a shallow, stratified water column and a major phytoplankton bloom. In these areas it is possible that phytoplankton growth and survival is adversely impacted by enhanced UV-B. In coastal areas, however, the sea ice, which attenuates most of the UV-B before it reaches the water column, remains until December/January, by which time UV-B levels have returned to long-term seasonal averages. Phytoplankton from these areas are unlikely to show long-term changes resulting from the hole in the ozone layer. Fjords of the Vestfold Hills, eastern Antarctica, have anoxic basins which contain high-resolution, unbioturbated sedimentary sequences. Diatom assemblages from these sequences reflect the diatom component of the phytoplankton and sea-ice algal assemblages at the time of deposition. Twenty-year records from these sequences show no consistent record of change in species composition, diversity or species richness. Six-hundred-year records from the same area also show changes in species abundance greater than those seen in the last 20 years. From these records it can be seen that recent changes in diatom abundances generally fall within the limits of natural variability and there is little evidence of recent changes that might be associated with UV-B-induced change.

A collection of about 20 isolates of Antarctic microalgae from the Windmill Islands region, around Casey Station has been established in the University of Malaya Algae Culture Collection (UMACC). The Antarctic microalgae in the collection includes Chlamydomonas, Chlorella, Stichococcus, Navicula. Ulothrix and Chlorosarcina. Comparative studies on the effect of global warming and UVR stress on these Antarctic microalgae and the tropical collection are being conducted. From the abstract of one of the referenced papers: The growth, biochemical composition and fatty acid profiles of six Antarctic microalgae cultured at different temperatures, ranging from 4, 6, 9, 14, 20 to 30 degrees C, were compared. The algae were isolated from seawater, freshwater, soil and snow samples collected during our recent expeditions to Casey, Antarctica, and are currently deposited in the University of Malaya Algae Culture Collection (UMACC). The algae chosen for the study were Chlamydomonas UMACC 229, Chlorella UMACC 234, Chlorella UMACC 237, Klebsormidium UMACC 227, Navicula UMAC 231 and Stichococcus UMACC 238. All the isolates could grow at temperatures up to 20 degrees C; three isolates, namely Navicula UMACC 231 and the two Chlorella isolates (UMACC 234 and UMACC 237) grew even at 30 degrees C. Both Chlorella UMACC 234 and Stichococcus UMAC 238 had broad optimal temperatures for growth, ranging from 6 to 20 degrees C (growth rate = 0.19 - 0.22 per day) and 4 to 14 degrees C (growth rate = 0.13 - 0.16 per day), respectively. In constrast, optimal growth temperatures for Navicula UMACC 231 and Chlamydomonas UMACC 229 were 4 degrees C (growth rate = 0.34 per day) and 6 to 9 degrees C (growth rate = 0.39 - 0.40 per day), respectively. The protein content of the Antarctic algae was markedly affected by culture temperature. All except Navicula UMACC 231 and Stichococcus UMACC contained higher amount of proteins when grown at low temperatures (6-9 degrees C). The percentage of PUFA, especially 20:5 in Navicula UMACC 231 decreased with increasing culture temperature. However, the percentages of unsaturated fatty acids did not show consistent trend with culture temperature for the other algae studied. There are three spreadsheets available in the download file. ASAC_2590 - provides detail about where each species of algae was collected from. ASAC_2590a - provides data from Teoh Ming-Li et al (2004) ASAC_2590b - provides data from Wong Chiew-Yen et al (2004) The fields in this dataset are: Isolate Culture Collection number Origin (Location) Fatty acids saturated fatty acids polyunsaturated fatty acids monounsaturated fatty acids Temperature growth rate PAR UVB

Metadata record for data from ASAC Project 492 See the link below for public details on this project. From the abstracts of the referenced papers: Diatom assemblages in two Holocene sediment cores (GC1 and GC2) from the Mac. Robertson Shelf, East Antarctica, are compared with modern sedimentary diatom assemblages from the same area. Open marine deposition commenced in Iceberg Alley (GC1), on the outer continental shelf, greater than 10.7 adj. 14C kyr BP. Chaetoceros resting spores, which may indicate water-column stabilsation from melting glacial and/or sea ice or the maximum summer sea-ice retreat, dominate the diatom assemblage. Approximately 7.5 adj. 14C kyr BP, a sea-ice diatom assemblage was deposited. This assemblage is similar to that being deposited in the surface sediments of the Mac. Robertson Shelf today and suggests that perennial sea ice has persisted in the vicinity of Iceberg Alley since that time. Interbedded within the sea-ice assemblage, however, are Corethron-rich sediment layers that suggest mid- to late-Holocene high-productivity events associated with a climatic optimum. The diatom record from Nielsen Basin (GC2), on the inner continental shelf, is relatively uniform compared to that in GC1. Glacial ice was present over the region c. greater than 5.6 adj. 14C kyr BP and a dissolution diatom assemblage was deposited beneath it. following ice retreat, an ice-edge diatom assemblage was deposited briefly before sea-ice conditions similar to that on the continental shelf today developed. There is no evidence in GC2 for the mid- to late-Holocene high-productivity events identified in GC1. Four diatom assemblages are identified from the surface sediments of Prydz Bay and the Mac. Robertson Shelf using multivariate analysis. A coastal assemblage is characterised by the sea-ice diatoms Fragilariopsis curta, F. angulata, F. cylindrus and Pseudonitzschia turgiduloides. A continental shelf assemblage is characterised by the open-water diatoms Fragilariopsis kerguelensis, Thalassiosira lenuginosa, T. gracilis var. expecta and Trichotoxin reinboldii. The Cape Darnley assemblage contains both sea-ice and open-water diatoms, but all are characteristically large and heavily silicified. Multiple regression has been used to identify the relationships between the diatom assemblages and known environmental variables. There are strong correlations between the coastal, shelf and oceanic assemblages and ecological conditions, including latitude, sea-ice distribution and ocean currents. The Cape Darnley assemblage is thought to represent an assemblage from which the smaller and more lightly silicified species have been removed by current winnowing. The palaeo-depositional environment of inner Prydz Bay, East Antarctica, has been reconstructed for the past 21,320 14C yr B.P., using diatom assemblages and sediment facies from a short, 352 cm long gravity core. Between 21,320 and 11,650 14C yr B.P., compact tillite and diamicton are present in the core, and diatom frustules are rare to absent. These data suggest that an ice sheet grounded over the site during the last glacial maximum. Following glacial retreat, siliceous muddy ooze was deposited, from 11,650 to 2600 14C yr B.P., in an open marine setting. During this stage, diatom frustules are abundant and well preserved, and Thalassiosira antarctica resting spores and Fragilariopsis curta dominate the assemblage. This assemblage suggests open marine deposition in an environment where the spatial and temporal distribution of sea ice is less than today. Since 2600 14C yr B.P., sea-ice and ice-edge diatom species have become more abundant, and neoglacial cooling is inferred. The assemblage is similar to that forming currently in Prydz Bay, where sea-ice is absent (less than 10% cover) for 2-3 months of the year and permanent ice edge and/or multiyear sea ice remains in close proximity to the site.

This data set was collected during an ocean acidification mesocosm experiment performed at Davis Station, Antarctica during the 2014/15 summer season. It includes: - description of methods for all data collection and analyses. - diatom cell volume - bulk silicification - species specific silicification via fluorescence microscopy - bulk community Fv/Fm on day 12 - single-cell PAM fluorometry data (maximum quantum yield of PSII: Fv/Fm) A natural community of Antarctic marine microbes from Prydz Bay, East Antarctica were exposed to a range of CO2 concentrations in 650 L minicosms to simulate possible future ocean conditions up to the year ~2200. Diatom silica precipitation rates were examined at CO2 concentrations between 343 to 1641 micro atm, measuring both the total diatom community response and that of individual species, to determine whether ocean acidification may influence future diatom ballast and therefore alter carbon and silica fluxes in the Southern Ocean. Described and analysed in: Antarctic diatom silicification diminishes under ocean acidification (submitted for review) Methods described in: Antarctic diatom silicification diminishes under ocean acidification (submitted for review) Location: Prydz bay, Davis Station, Antarctica (68 degrees 35'S, 77 degrees 58' E) Date: Summer 2014/2015 Worksheet descriptions: Bulk silicification - raw data Measured total and incorporated biogenic silica using spectrophotometer for all tanks on day 12 after 24 h incubation with PDMPO - raw data Bulk Fv/Fm - dark-adapted maximum quantum efficiency of PSII (Fv/Fm) on whole community - raw data Measured Fv/Fm of individual cells from 3 mesocosm tanks. Single-cell silicificiation, Fluorescence microscopy - raw data Measured autofluorescence and PDMPO fluorescence of individual diatoms from 6 mesocosm tanks Single-cell PAM, dark-adapted maximum quantum efficiency of PSII (Fv/Fm) - raw data Measured Fv/Fm of individual cells from 3 mesocosm tanks. Cell volume Calculated cell volume (um3) of 7 species from minicosm tanks 1 and 6 - raw data Abbreviations: Fv/Fm Maximum quantum yield of PSII PDMPO 2-(4-pyridyl)-5-((4-(2-dimethylaminoethylaminocarbamoyl)methoxy)phenyl)oxazole Tant Thalassiosira antarctica DiscLg Large Discoid centric diatoms Stella Stellarima microtrias Chaeto Chaetoceros spp. Prob Proboscia truncata Pseu Pseudonitzschia turgiduloides FragLg Fragilariopsis cylindrus / curta Centric Large Discoid centric diatoms LargeThalassiosira Large Discoid centric diatoms

The actual piece of equipment used was an International Light IL 1700Radiometer equipped with broad band detectors to measure PAR, UV-A and erythemal UV-B. The effects of UV-B radiation on the fatty acid, total lipid and sterol composition and content of three Antarctic marine phytoplankton were examined in a preliminary culture experiment. Exponential growth phase cultures of the diatoms Odontella weissflogii and Chaetoceros simplex and the Haptophyte Phaeocystis antarctica were grown at 2 (plus or minus 1)degrees C and exposed to 16.3 (plus or minus 0.7) W.m-2 photosynthetically active radiation (PAR). UV-irradiated treatments were exposed to constant UV-A (4.39 (plus or minus 0.20) W.m-2) and low (0.37 W.m-2) or high UV-B (1.59 W.m-2). UV-B treatments induced species specific changes in lipid content and composition. The sterol, fatty acid and total lipid content and profiles for O. weissflogii changed little under low UV-B when compared with control conditions (PAR alone), but showed a decrease in the lipid content per cell under high UV-B treatment. In contrast, when P. antarctica was exposed to low UV-B irradiance, storage lipids were reduced and structural lipids increased indicating that low UV-B enhanced cell growth and metabolism. P. antarctica also contained a higher proportion of polyunsaturated fatty acids under low UV-B in comparison with PAR irradiated control cultures. The flagellate life stage of P. antarctica died under high UV-B irradiation. However, exposure of P. antarctica to high UV-B irradiance increased total lipid, triglyceride and free fat. The effect of UV-B irradiances on the lipid content of Antarctic marine phytoplankton is species specific. Changes in ambient UV-B may alter the nutritional quality of food available to higher trophic levels. EXPERIMENTAL All measurements of irradiance were made with an International Light IL 1700 Radiometer equipped with broad band detectors to measure PAR, UV-A and erythemal UV-B [14]. A National Institute of Standards and Technology intercomparison package (NIST Test #534/240436-88) was used to calibrate each light sensor. Unialgal cultures of the diatoms Odontella weissflogii and Chaetoceros simplex were isolated from sea ice collected in Prydz Bay, Antarctica during the 1990/91 austral summer. Phaeocystis antarctica was isolated from Prydz Bay in 1982/83 summer. Cultures of diatoms and Phaeocystis antarctica were maintained in 2 l glass flasks using f/2 growth medium [32] and GP5 medium [33] respectively at a temperature of 2 plus or minus 1 degrees C. Cool white fluorescent lights provided photosynthetically active radiation (PAR) intensity of 17.08 J.m-2.s-1 (84.7 micro E.m-2.s-1), with no UV-B enhancement, on a 12 h light : 12 h dark cycle. Immediately before experimental irradiation, three replicate subsamples of approximately 15 ml were obtained from each parental culture and fixed with Lugols iodine, a known sample volume sedimented, and cells counted over 15 replicate fields using a Labovert inverted microscope. Mean cell concentration and standard deviation were then computed. Each exponential growth phase parental culture was thoroughly mixed and 3 replicate 300 ml Costar polystyrene culture flasks (which completely absorbed wavelengths below 295 nm) established for each light treatment (control, low and high UV exposures). Cultures were irradiated for 24 hours in a 48 hour experimental period (6 h light : 12 h dark : 12 h light : 12 h dark : 6 h light) [14, 23]. Exposures were conducted in a Thermoline controlled environment cabinet at 2 plus or minus 1 degrees C with cool white fluorescent tubes to provide PAR and UV-A (320-400 nm), with UV-B provided by FS20T 12 UV-B Westinghouse sunlamps. PAR and UV-A irradiances were 16.3 plus or minus 0.7 W.m-2 (81.3 plus or minus 3.4 micro E.m-2.s-1) and 4.39 plus or minus 0.20 W.m-2 respectively. The spectral distribution and UV-B irradiance were varied by attenuation with glass filters [5] to provide low (0.37 W.m-2) or high UV-B (1.59 W.m-2). Sensors were each covered by an attenuating glass screen and a single layer of Costar culture flask to measure the experimental irradiances to which the algae were exposed. UV-B irradiances were chosen to reflect less than (74%) and greater than (318%) peak UV-B exposure as measured at an Antarctic coastal site (Casey station, 66 degrees S, [34]). Following irradiation each culture was well mixed and approximately 15 ml was fixed with Lugols Iodine for subsequent estimation of cell concentration (as above). Chlorotic and greatly vesicularised cells were considered to be dead [23]. The remainder of each experimental culture was filtered through Whatman GF/F filters. On completion of filtration, the filters were stored at -20C overnight before extraction of lipids the following day.

Antarctic lake cores record a history of precipitation in the preservation of climate sensitive microbial communities. Comprehensive integration of our precipitation records with other climate proxies such as ice core temperature records and historical climate data are dependent upon accurate dating of this lake sediment. Fourteen lakes and ponds of the Windmill Islands were sampled in 1998 for diatoms and in 1999 for water chemistry. The waterbodies included in this study fall into one of 3 broad categories: saline lake (greater than 5m deep; greater than, or equal to, 3 parts per thousand - salinity), saline pond (less than 5m deep; greater than, or equal to, 3 parts per thousand - salinity) or freshwater pond (less than 5m deep; less than 3 parts per thousand - salinity). Saline Lakes Beall Lake, the largest lake on Beall Island, is situated in a rocky catchment with evidence of breeding penguin pairs nearby. Outflow into the small lake on the northwestern point of Beall Lake occurs at elevated lake levels. Holl Lake, the largest lake on Holl Island, is contained by ridges to the NE and SW with an obvious outflow to the SE. At the time of sampling (20 Dec 1998), penguin feathers were observed in the sediment. In 2001 large numbers of penguins were observed behind the NE ridge in addition to the numerous skuas nesting on most nearby peaks. Lake A is the westernmost lake on Browning Peninsula. This large closed saline lake has a very thick ice cover (~2.5 m) and very little evidence of birdlife. Lake M is the easternmost lake sampled on Browning Peninsula. This large closed saline lake had a very thick ice cover (3.0 m) at the time of sampling. Saline Ponds Lake Warrington is the largest waterbody on Warrington Island. Found in the centre of Warrington Island, this small shallow (1.9 m) saline pond was almost completely frozen (ice cover of 1.6 m), with ca. 0.3 m of water below the ice at the time of sampling. The lake catchment is muddy with runoff towards Robertson Channel (to the NE) and the ice cover showed signs of sediment entrapment giving a gritty texture. Lake G is located on northeastern Peterson Island. This very saline (greater than 60 ppt) shallow (1.0 m) pond was almost completely frozen (ice cover of 0.8 m), with ca. 0.1 m of water below the ice at the time of sampling. Lake G is close to breeding penguin sites and there was a noticeable discolouration of the surface water at the time of sampling. Lake I is the easternmost of the three sites visited on southern Peterson Island. This shallow (0.3 m) saline pond is very close to breeding penguin sites and was sampled by hand as the ice cover (0.1 m) was almost as thick as the lake depth. Lake K is approx. 400 m to the west of Lake I on central southern Peterson Island. This completely frozen saline pond is also very close to breeding penguin sites. Lake L is the southernmost pond sampled on Peterson Island. This almost completely frozen shallow (~0.8 m/0.8 m ice cover) saline pond is very close to breeding penguin sites with noticeable discolouration of the top ca. 0.2 - 0.3 m of water at the time of sampling. Freshwater Ponds Lake B, a shallow (0.9 m) freshwater pond, is located on the western side of Browning Peninsula, approx. 500 m to the south of Lake A. Lake C is a shallow (1.0 m) freshwater pond in the central valley of Browning Peninsula. Lake D is a shallow (0.5 m) freshwater pond in the central valley of Browning Peninsula approx. 500 m to the north of Lake C. This lake was sampled by hand as the ice cover (~0.5 m) was almost as thick as the lake depth. Lake E is a shallow (3.1 m) freshwater pond in the central valley of Browning Peninsula approx. 250 m to the north of Lake D. Lake F is the northernmost pond sampled from the central valley of Browning Peninsula. This freshwater pond is approx. 500 m to the north-west of Lake E. The sediment/species samples were collected in November and December 1998, the water samples were collected in December 1999. The fields in this dataset are: Lake Name Code Location Latitude Longitude Lake Depth Ice Depth Water Sample Salinity Lake Area Catchment Elevation Nitrite Nitrate Silicon Phosphate pH Species The numbers given in the species spreadsheet are for percentage abundance, ie the relative abundance of each species in the community.

Ellis Fjord is a small, fjord-like marine embayment in the vestfold Hills, eastern Antarctica. Modern sediment input is dominated by a biogenic diatom rain, although aeolian, fluvial, ice-rafted, slumped and tidal sediments also make a minor contribution. In areas where bioturbation is significant relict glaciogenic sediments are reworked into the fine-grained diatomaceous sediments to produce poorly sorted fine sands and silts. Where the bottom waters are anoxic, sediments remain unbioturbated and have a high biogenic silica component. Three depositional and non-depositional facies can be recognised in the fjord: an area of non-deposition around the shoreline; a relict morainal facies in areas of low sedimentation and high bioturbation; and a basinal facies in the deeper areas of the fjord.

A sediment core was collected from the western side of Pidgeon Island, (66.3216 S, 110.445 E) at a water depth of 82.0 m. This sediment core (PG 1411-2) was recovered using a release-controlled piston corer, with a length of 3 m, using the coring technique described in Melles et al., (1994). The total core length was 240 cm. This core was stored in the dark, at 0 degrees C until required. Samples were taken for diatom analyses and radiocarbon (14C) dating. Prior to sub-sampling the core was split in half, along its length. One half was used for sampling, the other kept intact and stored at IASOS (University of Tasmania). To reduce potential contamination, resulting from the disturbance of sediments during the core-splitting procedure, a thin layer of sediment was removed from the exposed surface immediately prior to sampling. In order to obtain samples for diatom analysis, a toothpick was inserted into the core segment, and used to gouge a small amount of sediment from the middle of the core. Samples for diatom analyses were initially collected every 5 mm, however, sampling frequency progressively decreased down the core. Samples for radiocarbon data consisted of at least 1 cm 3 of sediment, collected from the middle of the core. These samples were collected from between 0-1 cm, 12-13 cm, 59-60 cm, 77-78 cm, 117-118 cm, and 229-230 cm depth. Diatom data are presented as raw counts, benthic abundances, the ratio of benthic to plankton species, and as the benthic index. Calculated ages (in years) are also given for all samples. The sedimentological core log is given as a powerpoint presentation. This work was completed as part of ASAC project 1130 (ASAC_1130) and project 2201 (ASAC_2201). Public summary from project 1130: Algal mats grow on sea floor in most shallow marine environments. They are thought to contribute more than half of the total primary production in many of these areas, making them a critical food source for invertebrates and some fish. We will establish how important they are in Antarctic marine environments and determine the effects of local sewerage and tip site pollution. We will also investigate the impact on the algal mats of the additional UV radiation which results from the ozone hole. Public summary from project 2201: As a signatory to the Protocol on Environmental Protection to the Antarctic Treaty Australia is committed to comprehensive protection of the Antarctic environment. This protocol requires that activities in the Antarctic shall be planned and conducted on the basis of information sufficient to make prior assessments of, and informed judgements about, their possible impacts on the Antarctic environment. Most of our activities in the Antarctic occur along the narrow fringe of ice-free rock adjacent to the sea and many of our activities have the potential to cause environmental harm to marine life. The Antarctic seas support the most complex and biologically diverse plant and animal communities of the region. However, very little is known about them and there is certainly not sufficient known to make informed judgements about possible environmental impacts. The animals and plants of the sea-bed are widely accepted as being the most appropriate part of the marine ecosystem for indicating disturbance caused by local sources. Attached sea-bed organisms have a fixed spatial relationship with a given place so they must either endure conditions or die. Once lost from a site recolonisation takes some time, as a consequence the structure of sea-bed communities reflect not only present conditions but they can also integrate conditions in the past. In contrast, fish and planktonic organisms can move freely so their site of capture does not indicate a long residence time at that location. Because sea-bed communities are particularly diverse they contain species with widely differing life strategies, as a result different species can have very different levels of tolerance to stress; this leads to a range of subtle changes in community structure as a response to gradually increasing disturbance, rather than an all or nothing response. This project will examine sea-bed communities near our stations to determine how seriously they are affected by human activities. This information will be used to set priorities for improving operational procedures to reduce the risk of further environmental damage. The fields in this dataset are: Species Site Benthic % Planktonic % Depth (cm) Age (years) Radiocarbon Age Corrected Age Benthic Index