This video is supplementary data for the publication entitled 'Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques'. The video is high resolution microscopy video of a live krill captured in the krill containment trap placed within the water bath. File size: 1.8 GB, 32 s duration. The optical microscopy was carried out using a Leica M205C dissecting stereomicroscope with a Leica DFC 450 camera and Leica LAS V4.0 software to collect high-resolution video. The experimental krill research project is designed to focus on obtaining life history information of use in managing the krill fishery - the largest Antarctic fishery. In particular, the project will concentrate on studies into impacts of climate change on key aspects of krill biology and ecology.

Aerial surveys of southern right whales (Eubalaena australis) were undertaken off the southern Australian coast to monitor the recovery of this endangered species following extreme 19th and 20th Century commercial whaling. The aerial survey was undertaken in the coastal waters from Perth (Western Australia) to Ceduna (South Australia) between the 12th and 17th August 2021, to maintain the annual series of surveys and inform the long-term population trend. The maximum whale counts for each leg of the survey flights between Cape Leeuwin and Ceduna, and consisted of a total 643 southern right whales sighted across the survey area (270 cow-calf pairs and 103 unaccompanied whales). The subsequent population estimate for the Australian ‘south-western’ population is 2,549 whales, which represents the majority of the Australian population given the very low numbers in the ‘south-eastern’ subpopulation. The population long-term trend data is indicating recent years (from 2007) are showing greater inter-annual variation in whale counts. To evaluate the recovery of the southern right whale population, it will be critical to collect long-term data on the annual variability in whale numbers related to the non-annual female breeding cycle and identify possible impacts on this by short-term climate dynamics, longer-term climate change and/or anthropogenic threats.

Metadata record for data from ASAC Project 1212. See the link below for public details on this project. ---- Public Summary from Project ---- This project aims to improve ship-based sea-ice thickness measurements made using an electromagnetic induction device by performing a theoretical analysis of the sensitivity of the electromagnetic instrument to factors such as instrument height and orientation, ice conductivity and thickness, and seawater conductivity. The results of the theoretical study will be used to assist the interpretation of an existing sea-ice thickness data set from the Mertz Glacier polynya cruise (V1, 1999/2000). The data set consists of the results of numerical modelling of the response of the EM31 electromagnetic instrument to typical one- and three-dimensional sea ice structures. One-dimensional model calculations were performed using software written specifically for the project. Three-dimensional model calculations were performed using Marco_air version 2.3, written by Z. Xiong and A. Raiche, CSIRO Mathematical Geophysics Group. Technical descriptions of this program are given in the preceding References section. The download file below contains some numerical output from the models, as well as a detailed description of the models used.

Tracking spring and summer migration of humpbacks from Eden NSW, Australia to Antarctica. Dataset can be found here: https://data.aad.gov.au/aadc/argos/display_campaign.cfm?campaign_id=70 Satellite tags were deployed on adult humpback whales with a modified version of the Air Rocket Transmitter System (ARTS, Restech) and a purpose-designed projectile carrier at a pressure of 7.5 – 10 bar. A custom-designed, 80mm anchor section is attached to a stainless steel cylindrical housing containing a location-only transmitter (SPOT-5 by Wildlife Computers, Redmond, Washington, USA and Kiwisat 202 Cricket by Sirtrack, Havelock North, New Zealand). This superseded anchor design resulted in the anchor section disarticulating upon deployment in order to achieve improved tag retention times while minimising impact. The tags were sterilised with ethylene oxide prior to deployment and implanted up to 290mm into the skin, blubber, interfacial layers and outer muscle mass of the whale. Tags were programmed to transmit to the Argos satellite system at various duty cycles and repetition rates for a maximum of 720 transmissions per day. These transmissions are relayed to processing centres which calculate the transmitter’s location by measuring the Doppler Effect on transmission frequency.

These aerial survey data of southern right whales (Eubalaena australis) off southern Australia were collected in August 2019. Such annual flights in winter/spring between Cape Leeuwin (Western Australia) and Ceduna (South Australia) have now been conducted over a 27-year period 1993-2019. These surveys have provided evidence of a population trend of around 6% per year, and a current (at 2014) population size of approximately 2300 of what has been regarded as the 'western' Australian right whale subpopulation. With estimated population size in the low thousands, it is presumed to be still well below carrying capacity. No trend information is available for the 'eastern' subpopulation of animals occurring around the remainder of the southern Australian Coast, to at least as far as Sydney, New South Wales and the populations size is relatively small, probably in the low hundreds. A lower than expected 'western' count in 2015 gives weak evidence that the growth rate may be starting to show signs of slowing, though an exponential increase remains the best description of the data. If the low 2015 count is anomalous, future counts may be expected to show an exponential increase, but if it is not, modelling growth as other than simple exponential may be useful to explore in future.

These aerial survey data of southern right whales (Eubalaena australis) off southern Australia were collected in August 2016. Such annual flights in winter/spring between Cape Leeuwin (Western Australia) and Ceduna (South Australia) have now been conducted over a 23-year period 1993-2016. These surveys have provided evidence of a population trend of around 6% per year, and a current (at 2014) population size of approximately 2300 of what has been regarded as the 'western' Australian right whale subpopulation. With estimated population size in the low thousands, it is presumed to be still well below carrying capacity. No trend information is available for the 'eastern' subpopulation of animals occurring around the remainder of the southern Australian Coast, to at least as far as Sydney, New South Wales and the populations size is relatively small, probably in the low hundreds. A lower than expected 'western' count in 2015 gives weak evidence that the growth rate may be starting to show signs of slowing, though an exponential increase remains the best description of the data. If the low 2015 count is anomalous, future counts may be expected to show an exponential increase, but if it is not, modelling growth as other than simple exponential may be useful to explore in future. A data update was provided in August, 2020 to correct some incorrectly given longitude values.

These aerial survey data of southern right whales (Eubalaena australis) off southern Australia were collected in September 2015. Such annual flights in winter/spring between Cape Leeuwin (Western Australia) and Ceduna (South Australia) have now been conducted over a 23-year period 1993-2015. These surveys have provided evidence of a population trend of around 6% per year, and a current (at 2014) population size of approximately 2300 of what has been regarded as the 'western' Australian right whale subpopulation. With estimated population size in the low thousands, it is presumed to be still well below carrying capacity. No trend information is available for the 'eastern' subpopulation of animals occurring around the remainder of the southern Australian Coast, to at least as far as Sydney, New South Wales and the populations size is relatively small, probably in the low hundreds. A lower than expected 'western' count in 2015 gives weak evidence that the growth rate may be starting to show signs of slowing, though an exponential increase remains the best description of the data. If the low 2015 count is anomalous, future counts may be expected to show an exponential increase, but if it is not, modelling growth as other than simple exponential may be useful to explore in future. A data update was provided in August, 2020 to correct some incorrectly given longitude values.

This study assessed the performance of diffusive gradients in thin-films (DGT) with a binding resin that used Chelex-100 (iminodiacetic acid functional groups) to measure cadmium, copper, nickel, lead, and zinc contaminants in Antarctic marine conditions. To do this, three sets of experiments were done: (I) the uptake of metals to DGT samplers was assessed over time when deployed to three metal mixtures of known concentrations (DGT performance page). This allowed for the determination of metal diffusion coefficients in Antarctic marine conditions and demonstrated when metal competition for binding sites were likely to occur. (II) the DGT were deployed in the presence of the microalga Phaeocystis antarctica at a concentration of 1000-3000 cells/mL to investigate how environmentally realistic concentrations of an Antarctic marine microalgae affect the uptake of metals (DGT uptake with algae page). Finally, the DGT-labile concentrations from part (II) were used in reference toxicity mixture models to predict toxicity to the microalgae so they could be compared to a previous study that investigated the toxicity of metal mixtures to Phaeocystis antarctica and Cryothecomonas armigera (DGT toxicity modelling page).

We use RNA sequencing to investigate which genetic/physiological pathways in Antarctic krill are affected by increased CO2 levels. We carried out larval CO2 exposure experiments in March 2012 at the AAD aquarium. Two developmental stages were used (Calyptopis I and Furcilia V) and three CO2 levels (control, 1000 and 2000 ppm). These were short term experiments (2 days) - since initial longer experiments starting with fertilized eggs resulted in differences in developmental stages between treatments and control which could confound the data. RNA was extracted from larvae and high-throughput RNA sequencing (RNA-seq) was carried out on 6 samples (2 stages * 3 treatments). Sequencing was carried out on an Illumina sequencer (Genome Analyzer II). We collected ~ 60 million sequence reads per sample (Data in FASTA format each read gives 100 base pairs of sequence), so a total of ~360 million reads (36 billion bp of data).

This data record has been compiled for a statistical methods study, conducted by Abigael Proctor as part of her PhD research in 2018. The data in this record have been used to showcase a new statistical method for determining no effect concentration (NEC). The study uses the data in this record to compare NEC and LCx estimates for copper in four Antarctic marine invertebrate species. The data associated with this record are a subset of four existing larger datasets: 1. amphipod: AAS_2933_Orchomenella_pinguides_Sensitivity_metals_Davis_2010-11 2. copepod: AAS_4100_Toxicity_Copepods 3. gastropod: AAS_2933_MetaToxicityMarine_JuvenileGastropods_Kingston2007 4. ostracod: AAS_2933_MetalToxicityMarine_BrownOstracods_Kingston2007 Subset details are described in the excel file provided.