Update - 2013-11-14 - data from the cruise now included in the download file. Two extra excel spreadsheets have been added to the download file - one is a summary file, and the other contains pigment data. Sampling sites with a list of activities at each site for the HIPPIES cruise of the Aurora Australis - December to January 2003-2004. Aurora V4 Tube Labelour use Sample#our use Date(UTC) Time(UTC)Shorthand entry code - ignore Time(UTC)Formatted Use this Long Degdegrees Long Minminutes Long Decdecimaldegrees Lat Degdegrees Lat Minminutes LatDecdecimaldegrees Local time (dec hrs)actual solar local time (decimal hours) calc from longitude DOES NOT EQUAL TIME ZONE Sea Temp Ice present/absent Lugol's#microscope sample number for phytoplankton ID (Our use only) HPLC Volvolume filtered for HPLC pigment analysis (Our use only) Cocco Volvolume filtered for coccolithophorid counts (Our use only) Cocco tray No.(Our use only) Location:DCM: Deep chlorophyll maximum Thermo: Thermocline

The data reports the pigment concentrations and results of CHEMTAX analysis for 2 summer seasons in Antarctic. In 2008/09 three experiments in which 6 x 650 l minicosms (polythene tanks) were used to incubate natural microbial communities (less than 200 um diameter) at a range of CO2 concentrations while maintained at constant light, temperature and mixing. The communities were pumped from ice-free water ~60 m offshore on 30/12/08, 20/01/09 and 09/02/09. These experiments received no acclimation to CO2 treatment. A further experiment was performed in 2014/15 using water helicoptered from ~ 1 km offshore amongst decomposing fast ice on 19/11/14. This experiment included a 5 day period during which the community was exposed top low light and the CO2 was gradually raised to the target value for each tank, followed by a two day period when the light was raised to an irradiance that was saturating but not inhibitory for photosynthesis. A range of coincident measurements were performed to quantify the structure and function of the microbial community (see Davidson et al. 2016 Mar Ecol Prog Ser 552: 93–113, doi: 10.3354/meps11742 and Thomson et al 2016 Mar Ecol Prog Ser 554: 51–69, 2016, doi: 10.3354/meps11803). The data provides a matrix of samples against component pigment concentration and the output from CHEMTAX that best explained the phytoplankton composition of the community based on the ratios of the component pigments. For the 2008/09 experiments, samples were obtained every 2 days for 10, 12 and 10 days in experiments 1, 2 and 3 respectively. In 2014/15 samples were obtained from each incubation tank on days 1,3, 5, and 8 during th acclimation period and every 2 days until day 18 thereafter. For each sample a measured volume was filtered through 13 mm Whatman GF/F filters for 20 mins. Filters were folded in half, blotted dry, and immediately frozen in liquid nitrogen for analysis in Australia. Pigments were extracted, analysed by HPLC, and quantified following the methods of Wright et al. (2010). Pigments (including Chl a) were extracted from filters with 300 micro l dimethylformamide plus 50 micro l methanol, containing 140 ng apo-8'-carotenal (Fluka) internal standard, followed by bead beating and centrifugation to separate the extract from particulate matter. Extracts (125 micro l) were diluted to 80% with water and analysed on a Waters HPLC using a Waters Symmetry C8 column and a Waters 996 photodiode array detector. Pigments were identified by comparing retention times and spectra to a mixed standard sample from known cultures (Jeffrey and Wright, 1997), run daily before samples. Peak integrations were performed using Waters Empower software, checked manually for corrections, and quantified using the internal standard method (Mantoura and Repeta, 1997).

Sampling sites with a list of activities at each site for the Tangaroa cruise - March to April 2004. Tangaroa Tube Label our use Sample#our use Date(UTC) Time(UTC)Shorthand entry code - ignore Time(UTC)Formatted Use this Long Degdegrees Long Minminutes Long Decdecimaldegrees Lat Degdegrees Lat Minminutes Lat Decdecimaldegrees Local time (dec hrs)actual solar local time (decimal hours) calc from longitude DOES NOT EQUAL TIME ZONE Sea Temp Ice present/absent Lugol's#microscope sample number for phytoplankton ID (Our use only) HPLC Volvolume filtered for HPLC pigment analysis (Our use only) Cocco Volvolume filtered for coccolithophorid counts (Our use only) Cocco tray No.(Our use only) Location:DCM: Deep chlorophyll maximum Thermo: Thermocline

Locations of sampling sites for ASAC project 40 on voyage 4 of the Aurora Australis in the 2009/2010 season. Samples were collected during March of 2010. The final dataset will contain information on chlorophyll, carotenoids, coccolithophorids and species identification and counts. Public Summary from the project: This program ... aims to determine the role of single celled plants, animals, bacteria and viruses in Antarctic waters. We quantify their vital role as food for other organisms, their potential influence in moderating global climate change through absorption of CO2 and production of DMS, and determine their response to effect of climate change. For more information, see the other metadata records related to ASAC project 40 (ASAC_40). The fields in this dataset are: Tube Label Date (UTC) Time (UTC) Latitude Longitude Sea Temperature Ice (Presence or Absence) Lugols Bottle Fluorometer

Locations of sampling sites for ASAC project 40 on voyage 6 of the Aurora Australis in the 1997/1998 season. Samples were collected during March of 1998. The final dataset will contain information on chlorophyll, carotenoids, coccolithophorids and species identification and counts. Public Summary from the project: This program ... aims to determine the role of single celled plants, animals, bacteria and viruses in Antarctic waters. We quantify their vital role as food for other organisms, their potential influence in moderating global climate change through absorption of CO2 and production of DMS, and determine their response to effect of climate change. For more information, see the other metadata records related to ASAC project 40 (ASAC_40). The fields in this dataset are: Tube Label Date (UTC) Time (UTC) Latitude Longitude Sea Temperature Ice (Presence or Absence) Lugols Bottle Fluorometer

Locations of sampling sites for ASAC project 40 on voyage 3 of the Aurora Australis in the 2006/2007 season. Samples were collected during January and February of 2007. The final dataset will contain information on chlorophyll, carotenoids, coccolithophorids and species identification and counts. Public Summary from the project: This program ... aims to determine the role of single celled plants, animals, bacteria and viruses in Antarctic waters. We quantify their vital role as food for other organisms, their potential influence in moderating global climate change through absorption of CO2 and production of DMS, and determine their response to effect of climate change. For more information, see the other metadata records related to ASAC project 40 (ASAC_40). The fields in this dataset are: Tube Label Date (UTC) Time (UTC) Latitude Longitude Sea Temperature Ice (Presence or Absence) Lugols Bottle Fluorometer

Locations of sampling sites for ASAC project 40 on rotation 4 of the French polar supply ship L'Astrolabe in the 2004/2005 season. Samples were collected between February and March of 2005. The final dataset will contain information on chlorophyll, carotenoids, coccolithophorids and species identification and counts. Public Summary from the project: This program aims to determine the role of single celled plants, animals, bacteria and viruses in Antarctic waters. We quantify their vital role as food for other organisms, their potential influence in moderating global climate change through absorption of CO2 and production of DMS, and determine their response to effect of climate change. For more information, see the other metadata records related to ASAC project 40 (ASAC_40). The fields in this dataset are: Tube label Date(UTC) Time(UTC) LongDecdecimaldegrees LatDecdecimaldegrees Local time (dec hrs)actual solar local time (decimal hours) calc from longitude DOES NOT EQUAL TIME ZONE Sea Tempdegrees Celsius Ice present/absent Lugol's#microscope sample number for phytoplankton ID (Our use only) HPLC Vol volume filtered for HPLC pigment analysis (Our use only) Cocco Vol volume filtered for coccolithophorid counts (Our use only) Cocco tray No.(Our use only) Net Sample Time

This dataset contains locations of sampling sites for ASAC project 40 on rotation 0 of the French polar supply ship L'Astrolabe in the 2007/2008 season. Samples were collected between October and November of 2007. It also contains information on chlorophyll, carotenoids, coccolithophorids and species identification and counts. Public Summary from the project: This program aims to determine the role of single celled plants, animals, bacteria and viruses in Antarctic waters. We quantify their vital role as food for other organisms, their potential influence in moderating global climate change through absorption of CO2 and production of DMS, and determine their response to effect of climate change. For more information, see the other metadata records related to ASAC project 40 (ASAC_40). The fields in this dataset are: Tube label Date(UTC) Time(UTC) Local time (dec hrs)actual solar local time (decimal hours) calc from longitude DOES NOT EQUAL TIME ZONE Longitude decimal degrees/degrees, minutes Latitude decimal degrees/degrees, minutes Sea Temperature degrees Celsius Ice present/absent Lugol's bottle # - microscope sample number for phytoplankton ID (Our use only) HPLC Volume remaining - volume filtered for HPLC pigment analysis (Our use only) Volume filtered for HPLC Volume remaining for Coccoliths Coccolith tray No.(Our use only) Coccolith tray position number Net Sample Time Fluorescence

This dataset contains locations of sampling sites for ASAC project 40 on rotation 0 of the French polar supply ship L'Astrolabe in the 2009/2010 season. Samples were collected between October and November of 2009. It also contains information on chlorophyll, carotenoids, coccolithophorids and species identification and counts. Public Summary from the project: ... This program aims to determine the role of single celled plants, animals, bacteria and viruses in Antarctic waters. We quantify their vital role as food for other organisms, their potential influence in moderating global climate change through absorption of CO2 and production of DMS, and determine their response to effect of climate change. For more information, see the other metadata records related to ASAC project 40 (ASAC_40). The fields in this dataset are: Tube Label Date (UTC) Time (UTC) Latitude Longitude Sea Temperature Ice (Presence or Absence) Coccolithophorid sample (yes or no) Plankton Net Sample Chlorophyll a (micro grams per litre)

This is a parent metadata record for work carried out as part of ASAC/AAS project 40. See the child metadata records for further information. More than 95% of the biomass in the Southern Ocean is microscopic - single celled plants, animals, bacteria and viruses. We are studying the factors that control their distribution and abundance - oceanographic and seasonal conditions, their physiology, and grazing - in order to model their vital roles as food for other organisms and their influence in moderating global climate change through absorption of CO2 and production of DMS. We are also addressing the changes expected in microbial communities through effects of climate change - global warming, sea ice retreat, ocean acidification and enhanced ultraviolet radiation. This project aims to determine the role of microorganisms in the Southern Ocean. The major objectives are to: * Identify and quantify key protistan components of the Southern Ocean ecosystem and study their autoecology. * Identify environmental and ecological processes that control abundance of key microbial components. * Determine interactions between key microbial components to quantify major pathways of carbon flow. * Determine the activity and viability of bacterioplankton and protists in the Southern Ocean. * Distinguish different microbial communities by identifying key taxa and associations so that processes such as primary production, respiration, grazing and particle flux can be readily parameterised in ecological models. * Determine the effect of elevated CO2 concentrations on microbial populations and processes. Taken from the 2008-2009 Progress Report: Progress against objectives: 1. Ongoing sampling from Astrolabe has continued, with 3 return voyages being sampled for phytoplankton species, chlorophyll a and other pigments, coccolithophorid counts and DNA profiles, in conjunction with measurements of CO2, ocean structure, fluorescence and ocean colour by CSIRO / CRC colleagues. 2. Three sets of minicosm experiments were conducted at Davis station with 7 staff spending 4.5 - 5.5 months on site. These experiments consistently found that acidification caused blooms of nanoplanktonic diatoms and increased bacterial activity, apparently by inhibition of microheterotroph grazers, at the expense of larger cells that are more readily ingested by grazers such as krill. We showed for the first time in Antarctic waters that pCO2 affects the structure and function Antarctic microbial communities in a way that may reduce food availability to large grazers. Over 100 cultures of "winners and losers" from such experiments were isolated and returned to Australia. These will form the basis for further physiological experiments including molecular assays. 3. Submission and acceptance of 8 papers from the BROKE-West cruise (5 as senior author). These showed the interactions between bottom-up (micronutient) top-down (grazing) control in structuring microbial populations in the marginal ice zone. Five biogeographic zones were identified on the basis of species composition, and the productivity was measured for each zone. Microzooplankton grazing experiments found that grazing within that microbial loop consumed a significant proportion of new productivity. In some areas later in the season, all productivity was consumed by microheterotrophs, rather than metazoans such as krill. A time sequence was identified for seeding and development of components of ice edge blooms, subsequent grazing and decline and a mechanism postulated for export of micronutrients (e.g. iron) by grazing and sedimentation that prevents subsequent development of surface water blooms and constrains populations to a deep chlorophyll maximum below the level of a nutricline. 4. Detailed analysis of greater than 30 strains of keystone species Emiliania huxleyi of two morphotypes in conjunction with Clara Hoppe (Masters student, Alfred Wegener Institute) and Suellen Cook (PhD student, University of Tasmania) showed consistent differences between strains in terms of pigmentation, responses to light and genetics. The two morphotypes appear to be adapted to different mixing regimes north and south of the Polar Front; the southern form may represent a new species. For a full list of references associated with this project, see the project link at the provided URL.