TemperateReefBase Geonetwork Catalogue
Enzyme analysis of long spined sea urchin (Centrostephanus rodgersii) on the south eastern seaboard of Australia
Sixty animals were collected from each of Bass Pt, New South Wales (lat 34°35' S, long 150°54' E; August 2000); south side of East Cove, Deal Is, Bass St. (lat 39°28.4' S, long 147°18.4' E; June 2000) and Fortescue Bay, Tasmania (lat 43°8.5' S, long 148°0.0' E; October 2000 and April 2001). To examine the genetic relationship between the three site populations of Centrostephanus rodgersii, allelic diversity and heterozygosity among the three sites was compared using BIOSYS.
Simple
Identification info
- Date (Creation)
- 2007-11-05T10:00:00
Principal investigator
School of Zoology, University of Tasmania (UTAS) - Johnson, Craig, Prof.
Private Bag 5
Hobart
TAS
7001
Australia
Collaborator
School of Zoology, University of Tasmania (UTAS) - Miller, Karen, Dr
Private Bag 5
Hobart
TAS
7001
Australia
- Purpose
- To determine whether newly established populations of the long spined sea urchin (Centrostephanus rodgersii) in Tasmania displayed lower levels of genetic variation relative to populations from mainland Australia, as might be expected if Tasmanian populations arose from a single or small number of founder events with little subsequent gene flow from other populations.
- Credit
- Funding: FRDC project 2001/044
- Status
- Completed
Principal investigator
Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Johnson, Craig, Prof.
IMAS, University of Tasmania
Private Bag 129
Hobart
TAS
7001
Australia
Collaborator
Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Miller, Karen, Dr
IMAS - Sandy Bay
Private Bag 129
Hobart
TAS
7001
Australia
- Topic category
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- Biota
Extent
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W
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Temporal extent
- Time period
- 2000-06-01T00:00:00 2001-04-01T00:00:00
Vertical element
- Minimum value
- 5
- Maximum value
- 18
- Identifier
- EPSG::5715
- Name
- MSL depth
- Maintenance and update frequency
- Not planned
Resource format
- Title
- Microsoft Excel (xls)
- Date
- Edition
- 2003
- CAAB - Codes for Australian Aquatic Biota v2.
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- 25 211001
- Centrostephanus rodgersii
- Keywords (Theme)
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- isozymes
- genetics
- Global Change Master Directory (GCMD) Earth Science Keywords Version 8.0
- Keywords (Discipline)
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- Temperate Reef
- Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research
Resource constraints
- Classification
- Unclassified
Resource constraints
- Use limitation
- The data described in this record are the intellectual property of the University of Tasmania through the School of Zoology and the Tasmanian Aquaculture and Fisheries Institute.
Resource constraints
- Linkage
-
http://i.creativecommons.org/l/by/2.5/au/88x31.png
License Graphic
- Title
- Creative Commons Attribution 2.5 Australia License
- Website
-
http://creativecommons.org/licenses/by/2.5/au/
License Text
- Other constraints
- The citation in a list of references is: citation author name/s (year metadata published), metadata title. Citation author organisation/s. File identifier and Data accessed at (add http link).
- Other constraints
- Consult attached report for a summary of the results. For further information please contact K. Miller.
- Language
- English
- Character encoding
- UTF8
- Environment description
- Microsoft Excel spreadsheet (xls)
- Supplemental Information
- Johnson, C., Ling, S., Ross, J., Shepherd, S. and Miller, K., 2005. Establishment of the long-spined sea urchin (Centrostephanus rodgersii) in Tasmania: First assessment of potential threats to fisheries. FRDC Final Report, Project No. 2001/044. University of Tasmania, Tasmanian Aquaculture and Fisheries Institute, School of Zoology. Johnson, CR and Banks, SC and Barrett, NS and Cazassus, FM and Dunstan, PK and Edgar, GJ and Frusher, SD and Gardner, C and Haddon, M and Helidoniotis, F and Hill, KL and Holbrook, NJ and Hosie, GW and Last, PR and Ling, SD and Melbourne-Thomas, J and Miller, KJ and Pecl, GT and Richardson, AJ and Ridgway, KR and Rintoul, SR and Ritz, DA and Ross, DJ and Sanderson, JC and Shepherd, SA and Slotwinski, A and Swadling, KM and Taw, N, Climate change cascades: Shifts in oceanography, species' range and subtidal marine community dynamics in eastern Tasmania, Journal of Experimental Marine Biology and Ecology, 400, (1-2) pp. 17-32. ISSN 0022-0981 (2011)
Distribution Information
- Distribution format
-
- Microsoft Excel (xls)
Distributor
- OnLine resource
- REPORT - FRDC final report [direct download]
- OnLine resource
-
imas:FR1_CRJohnson_Analysis_enzyme_long_spined_sea_urchin_GV
MAP - Sites of urchin collection
Resource lineage
- Statement
- Samples of gonad (free of other tissue) were excised from live animals, snap frozen in liquid nitrogen and stored at -80° C. Samples were screened initially for 18 enzyme systems. Five (ADH, AK, GPDH, LDH and 6PGDH) were eliminated from further investigation due to failure to produce detectable banding. Of the remaining 13, seven (AAT, APK, IDH, MDH, MPI, PGM and PGI) provided clearly interpretable and consistently repeatable patterns. Of these, three (APK, IDH, MDH) were monomorphic. The remaining four enzyme systems (AAT, MPI, PGM and PGI) were selected for use in the primary study. MPI, PGM and PGI were all run in the tris-glycene buffer (pH 8.5) and provided one polymorphic locus each. Electrophoretic runs using a tris-glyine buffer were carried out at room temperature over 30 minutes, while those using tris-citrate were run at 4°C for 65 minutes. Protocols for cellulose acetate electrophoresis were after Richardson et al (1986).
- Hierarchy level
- Dataset
Metadata
- Metadata identifier
- c12bac10-8b33-11dc-8a3c-00188b4c0af8
- Language
- English
- Character encoding
- UTF8
Point of contact
Type of resource
- Resource scope
- Dataset
- Metadata linkage
-
https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/c12bac10-8b33-11dc-8a3c-00188b4c0af8
Point of truth URL of this metadata record
- Date info (Creation)
- 2021-03-30T21:10:36
- Date info (Revision)
- 2021-03-30T21:10:36
Metadata standard
- Title
- ISO 19115-3:2018
Overviews
Spatial extent
N
S
E
W
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S
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W
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S
E
W
Provided by
Associated resources
Not available