Oxygen and nitrogen isotopic composition of nitrate in the Antarctic ice-edge, sea-ice and underlying seawater data collected during the SIPEX II voyage of the Aurora Australis, 2012

Overview of the project and objectives:


To investigate whether nitrate uptake and processes other than nitrate uptake by phytoplankton are significant and show spatial variability possibly induced by varying availability of Fe and other parameters in the region, seawater was collected from CTD (Conductivity, Temperature and Depth) and TMR (Trace Metals Rosette) casts jointly with the nutrient sampling, as well as well as sea-ice collected from Bio ice-core types on Ice Station, for analysis of nitrate d15N, d18O isotopic composition. Results have been interpreted in the light of prevailing nitrate-nutrient concentrations (Belgian team) and N-uptake regimes for the Ice Stations (new vs. regenerated production and nitrification; see Silicon, Carbon and Nitrogen in-situ incubation Metadata file).


Methodology and sampling strategy:


Samples for isotopic composition of nitrate were collected from the CTD rosette, TMR and Bio ice-core jointly with the nutrient sampling. Sea-ice sampling: sampling strategy follows ice stations deployment via Bio ice-core type. Most of the time we worked close to / directly on the Trace Metal site following precautions concerning TM sampling (clean suits etc.). When we worked close to the TM site, precautions were not such important because we don't need the same drastic precautions for our own sampling. We work together because we want to propose a set of data which helps to characterize the system of functioning in close relation with TM availability (for that, sampling location have to be as close as possible). All samples were filtered on 0.2 microns acrodiscs and kept at -20 degrees C till analysis in the home-based laboratory. We applied the denitrifier method elaborated by Sigman et al. (2001) and Casciotti et al. (2002). This method is based on the isotopic analysis of delta 15N and delta 18O of nitrous oxide (N2O) generated from nitrate by denitrifying bacteria lacking N2O-reductase activity. As a prerequisite the nitrate concentrations need to be known (nutrients analysis in the home lab.) as this sets sample amount provided to the denitrifier community. Briefly, sample nitrate is reduced by a strain of denitrifying bacteria (Pseudomonas aureofaciens) which transform nitrate into N2O, but lack the enzyme to produce N2. N2O is then analysed for N, O isotopic composition by IRMS (Delta V, Thermo) after elimination of CO2, volatile organic carbon and further cryogenic focusing of N2O (Mangion, 2011).


Casciotti K.L., D.M.Sigman, M.G. Hastings, J.K. Bohlke and A. Hilkert, 2002. Measurement of the oxygen isotopic composition of nitrate in seawater and freshwater using the denitrifier method, Analytical Chemistry, 74 (19): 4905-4912.


Mangion P., 2011. Biogeochemical consequences of sewage discharge on mangrove environments in East Africa, PhD Thesis, Vrije Universiteit Brussel, 208 pp.


Sigman D.M., Casciotti K.L., Andreani M., Barford C., Galanter M. and J.K. Bohlke, 2001. A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater, Analytical Chemistry, 73: 4145-4153.

Simple

Identification info

Alternate title
Oxygen and nitrogen isotopic composition of nitrate in the Antarctic ice-edge, sea-ice and underlying seawater data collected during the SIPEX II voyage of the Aurora Australis, 2012
Date (Publication)
2013-05-08
Edition
1

Originator

Dehairs, F., Roukaerts, A., Cavagna, A., Lannuzel, D. and Meiners, K.

Publisher

Australian Antarctic Data Centre

Principal investigator

DEHAIRS, FRANK
Vrije Universiteit Brussel, Pleinlaan 2
B-1050 Brussels
Belgium

Principal investigator

ROUKAERTS, ARNOUT
Vrije Universiteit Brussels
Laboratory of Analytical and Environmental Chemistry
Pleinlaan 2
Brussels
B-1050
Belgium

Principal investigator

CAVAGNA, ANNE-JULIE
Vrije Universiteit Brussel
Laboratory of Analytical and Environmental Chemistry
Pleinlaan 2
Brussels
B-1050
Belgium

Principal investigator

LANNUZEL, DELPHINE
University of Tasmania
Hobart
Tasmania
7001
Australia

Principal investigator

MEINERS, KLAUS
Sea-ice Ecologist
Antarctic Climate and Ecosystems Cooperative Research Centre (ACE CRC)
Private Bag 80
Hobart
Tasmania
7001
Australia

Collaborator

DEHAIRS, FRANK
Vrije Universiteit Brussel, Pleinlaan 2
B-1050 Brussels
Belgium

Collaborator

ROUKAERTS, ARNOUT
Vrije Universiteit Brussels
Laboratory of Analytical and Environmental Chemistry
Pleinlaan 2
Brussels
B-1050
Belgium

Collaborator

CAVAGNA, ANNE-JULIE
Vrije Universiteit Brussel
Laboratory of Analytical and Environmental Chemistry
Pleinlaan 2
Brussels
B-1050
Belgium

Collaborator

LANNUZEL, DELPHINE
University of Tasmania
Hobart
Tasmania
7001
Australia

Collaborator

MEINERS, KLAUS
Sea-ice Ecologist
Antarctic Climate and Ecosystems Cooperative Research Centre (ACE CRC)
Private Bag 80
Hobart
Tasmania
7001
Australia
Name
CAASM Metadata
Website
https://data.aad.gov.au/metadata/records/SIPEX_II_Stable_Isotopes_NO3

Status
Completed

Custodian

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Topic category
  • Oceans

Extent

N
S
E
W


Extent

Description
Temporal Coverage

Temporal extent

TimePeriod
2012-09-25 2012-11-01
NASA/GCMD Earth Science Keywords
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITRATE
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITROGEN
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > OXYGEN
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > STABLE ISOTOPES
Keywords
  • Isotopic composition
NASA/GCMD Earth Science Keywords
  • CTD > Conductivity Temperature Depth
  • CORING DEVICES
NASA/GCMD Earth Science Keywords
  • R/V AA > R/V Aurora Australis
  • LABORATORY
NASA/GCMD Earth Science Keywords
  • AMD
  • AMD/AU
  • CEOS
  • ACE/CRC
NASA/GCMD Earth Science Keywords
  • CONTINENT > ANTARCTICA
  • OCEAN > SOUTHERN OCEAN
  • GEOGRAPHIC REGION > POLAR

Resource constraints

Use limitation
This metadata record is publicly available.

Resource constraints

Access constraints
licence
Other constraints
These data are linked to the parent record SIPEX_II_Stable_Isotopes

Resource constraints

File type
Portable Network Graphic
Linkage
Creative Commons by Attribution logo

Title
Attribution 4.0 International (CC BY 4.0)
Website
https://creativecommons.org/licenses/by/4.0/legalcode

Legal code for Creative Commons by Attribution 4.0 International license

Use constraints
licence
Other constraints
This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SIPEX_II_Stable_Isotopes_NO3 when using these data.
Language
English
Character encoding
UTF8

Distribution Information

Distributor

Distributor

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
OnLine resource
PROJECT HOME PAGE

Public information for ASAC project 4051

OnLine resource
PROJECT HOME PAGE

Public information for ASAC project 4073

OnLine resource
VIEW RELATED INFORMATION

Citation reference for this metadata record and dataset

Resource lineage

Statement
Each eventual parameter important for global comprehension of the dataset is highlighted as comment, when needed, in the Final Sample List.xls file (See parent record).
Hierarchy level
Dataset
Maintenance and update frequency
As needed
Maintenance note
2013-05-09 - record created by Dave Connell from a template completed by Anne-Julie Cavagna. 2019-07-15 - record updated by Dave Connell - basic updates.

Metadata

Metadata identifier
string/SIPEX_II_Stable_Isotopes_NO3

Language
English
Character encoding
UTF8

Author

CAVAGNA, ANNE-JULIE
Vrije Universiteit Brussel
Laboratory of Analytical and Environmental Chemistry
Pleinlaan 2
Brussels
B-1050
Belgium

Sponsor

Australian Antarctic Division

Owner

AADC
Title
Parent Metadata Record
Citation identifier
SIPEX_II,SIPEX_II_Stable_Isotopes

Type of resource

Resource scope
Dataset

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
7cd43845-bdc7-49a1-ae27-9d160c5fb349

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Date (Last Revision)
2019-07-15T14:23:54

Identifier

Description
metadata.extraction_date

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
8.6

Metadata linkage
http://data.aad.gov.au/metadata/records/SIPEX_II_Stable_Isotopes_NO3

Point of truth for the metadata record

Date info (Creation)
2013-05-09T00:00:00
Date info (Last Update)
2019-07-15

Metadata standard

Title
ISO 19115-3
Edition
2014
Other citation details
Version 1
Title
DIF to ISO 19115-1 Profile
 
 

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Spatial extent

N
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