Nella Dan: SIBEX II Cruise Phytoplankton Data

This dataset contains results from the Second International BIOMASS Experiment II (SIBEX II) cruise of the Nella Dan, taken in January 1985. This cruise was the fourth cruise in a series of six. Phytoplankton samples were taken off Antarctica in the Australian sector (Mawson to Davis region) and Prydz Bay in January 1985. Taxonomic identity, distribution and abundance data were obtained, together with an extensive range of pigment analysis, using high performance liquid chromatography (HPLC). Over 60 pigments were analysed (only the major ones are listed here). The major phytoplankton investigated were diatoms, dinoflagellates and flagellates. This dataset is a subset of the full cruise.


An excel spreadsheet containing the full pigment analysis obtained from the cruise is available for download from the URL given below. The spreadsheet is a digital version of the data presented in ANARE Research Notes 58, which was a report written based on this dataset. There are three worksheets to the spreadsheet:


1) Abbrev. - details the abbreviations used in worksheets 2 and 3.

2) Table 3 - Table 3 data entered from ANARE Research Notes 58.

3) Transposed Table 3 - The same data as worksheet 2, but arranged differently.


A pdf copy of ANARE Research Notes 58 is also available for download at the URL given below. A paper written in 2006 about pigments in microalgae, which provides some up-to-date explanations about pigmentation, is also available for download, but owing to copyright restrictions, is only available for download by Australian Antarctic Division personnel.


The fields in this dataset are:


Date

Time (GMT)

Latitude

Longitude

Depth (metres)

Pigment concentration (nanograms per litre)

chlorophyllide a

chlorophyll c

methyl chlorophyllide a

phaeophorbide a

peridinin

19'-butanoyloxyfucoxanthin

fucoxanthin

19'-hexanoyloxyfucoxanthin

Neoxanthin

Prasinoxanthin

Violaxanthin

Diadinoxanthin

Alloxanthin

diatoxanthin

Zeaxanthin

Canthaxanthin

Unknown

Chlorophyll b

Chlorophyll a allomer

Chlorophyll a

Chlorophyll a epimer

Phaeophytin a derivative

Phaeophytin b

Phaeophytin a

Chlorophyll a total

% Degradation

Pigment total


This work was completed as part of ASAC project 40 (ASAC_40).

Simple

Identification info

Alternate title
Nella Dan: SIBEX II Cruise Phytoplankton Data
Date (Publication)
2007-04-19
Edition
1
Citation identifier
Dataset DOI

Title
Information and documentation - Digital object identifier system
Date (Publication)
2012-04-23
Citation identifier
ISO 26324:2012

Citation identifier
doi:10.26179/5c8ecd198c0e4

Originator

Wright, S.

Publisher

Australian Antarctic Data Centre

Principal investigator

WRIGHT, SIMON
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Collaborator

WRIGHT, SIMON
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Name
CAASM Metadata
Website
https://data.aad.gov.au/metadata/records/SIBEX_II_phyto

Status
Completed

Custodian

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Topic category
  • Biota
  • Oceans

Extent

N
S
E
W


Extent

Description
Temporal Coverage

Temporal extent

TimePeriod
1985-01-03 1985-01-26

Extent

Description
Vertical Extent - Depth

Vertical element

Minimum value
0
Maximum value
602
Identifier
urn:x-ogc:def:cs:EPSG:6498
Name
Vertical CS. Axis: depth (D). Orientation: down. UoM: m.
Identifier
urn:x-ogc:def:axis:EPSG:113
Name
depth
Title
Phytoplankton pigment data: Prydz Bay region - SIBEX II, MV Nella Dan , January 1985.
Date (Publication)
1987

Author

Wright, S.W.
Name
ANARE Research Notes
Issue identification
58
Page
102
Title
Photosynthetic pigments in marine microalgae: Insights from cultures and the sea.
Date (Publication)
2006

Author

Jeffrey, S.W., Wright, S.W.
Page
34-90
Other citation details
In: Subba Rao, D.V. (ed.) Algal Cultures, Analogues of Blooms and Applications
NASA/GCMD Earth Science Keywords
  • EARTH SCIENCE > BIOSPHERE > VEGETATION > CHLOROPHYLL
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > FLAGELLATES
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > FLAGELLATES > DINOFLAGELLATES
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE > DIATOMS
  • EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PIGMENTS > CHLOROPHYLL
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE > DINOFLAGELLATES
Keywords
  • Date
  • Time
  • Latitude
  • Longitude
  • Depth
  • Pigment concentration
  • chlorophyllide a
  • chlorophyll c
  • methyl chlorophyllide a
  • phaeophorbide a
  • peridinin
  • 19'-butanoyloxyfucoxanthin
  • fucoxanthin
  • 19'-hexanoyloxyfucoxanthin
  • Prasinoxanthin
  • Neoxanthin
  • Diadinoxanthin
  • Violaxanthin
  • Alloxanthin
  • diatoxanthin
  • Zeaxanthin
  • Canthaxanthin
  • Unknown
  • Chlorophyll b
  • Chlorophyll a allomer
  • Chlorophyll a
  • Chlorophyll a epimer
  • Phaeophytin a derivative
  • Phaeophytin b
  • Phaeophytin a
  • Chlorophyll a total
  • % Degradation
  • Pigment total
NASA/GCMD Earth Science Keywords
  • CTD > Conductivity, Temperature, Depth
  • HPLC > High-Performance Liquid Chromatograph
  • NISKIN BOTTLES
NASA/GCMD Earth Science Keywords
  • SHIPS
NASA/GCMD Earth Science Keywords
  • CEOS
  • AMD
  • AMD/AU
NASA/GCMD Earth Science Keywords
  • OCEAN > SOUTHERN OCEAN
  • GEOGRAPHIC REGION > POLAR

Resource constraints

Use limitation
This metadata record is publicly available.

Resource constraints

Access constraints
licence
Other constraints
An excel spreadsheet of data are available for download from the URL given below. A pdf copy of ANARE Research Notes 58 is also available for download from the URL given below. A pdf copy of a 2006 paper detailing pigmentation is available for download by Australian Antarctic Division personnel only.

Resource constraints

File type
Portable Network Graphic
Linkage
Creative Commons by Attribution logo

Title
Attribution 4.0 International (CC BY 4.0)
Website
https://creativecommons.org/licenses/by/4.0/legalcode

Legal code for Creative Commons by Attribution 4.0 International license

Use constraints
licence
Other constraints
This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SIBEX_II_phyto when using these data.
Language
English
Character encoding
UTF8

Distribution Information

Distributor

Distributor

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Distributor

Fees
free
Planned available datetime
2007-04-19T00:00:00

Distributor

Fees
free
Planned available datetime
2007-04-19T00:00:00

Distributor

Fees
free
Planned available datetime
2007-04-19T00:00:00
Units of distribution
kb
Transfer size
142
Distribution format
  • Excel

Units of distribution
MB
Transfer size
10.4
Distribution format
  • pdf

Units of distribution
MB
Transfer size
4.4
Distribution format
  • pdf

OnLine resource
GET DATA

Download point for the excel spreadsheet

OnLine resource
PROJECT HOME PAGE

Public information for ASAC project 40

OnLine resource
VIEW RELATED INFORMATION > PUBLICATIONS

Download point for ANARE Research Notes 58

OnLine resource
VIEW RELATED INFORMATION > PUBLICATIONS

Download point for the 2006 Jeffrey and Wright Paper

OnLine resource
VIEW RELATED INFORMATION

Citation reference for this metadata record and dataset

Resource lineage

Statement
This voyage is one of six which studied the abundance and distribution of both phytoplankton and Euphausia superba. They provided a good coverage of the area at varying season times and conditions. Data collection: Seven north-south transects were made between 58 degrees and 93 degrees east, with water samples collected at intervals of 5 degrees longitude. A General Oceanics rosette sampler equipped with twelve 5L Niskin botles was used for water collection in the top 200m of the water column. Standard depths for pigment analysis were used; 0, 10, 25, 35, 50, 75, 100, 200m. Temperature and salinity in the vertical profile were also measured, using a Neil Brown Mark 3 CTD. Ship-board processing: The water samples were analysed for oxygen, salinity, nutrients, and phytoplankton species. The remaining 2L to 4L of the water sample were filtered under a slight vacuum (approx. 0.5 atm.) using Whatman GF/F filters (0.7 micro-metre nominal pore size, 47mm diameter), and the filters were then immediately frozen in liquid nitrogen. Post-ship processing: The frozen filter was broken into 5mm diameter pieces, and sonicated in 4mL methanol using a Braun Labsonic 1510 sonicator equipped with a 4mm diameer needle probe (30 seconds at 50W). It was then filtered using a simple centrifugal system, described in Wright, S. and Shearer, J. (1984) Rapid extraction and high-performance liquid chromatography of chlorophylls and cartenoids from marine plankton. Journal of Chromatography 294:281-295. The filter debris was washed with 0.5mL methanol and recentrifuged. The combined extract was filtered through a Millex-HV filter unit (0.5 micro-metre pore size, Millipore Corp.). High performance liquid chromatography (HPLC) was used for pigment analysis. A 200 micro-litre sample of the extract was injected directly into a Waters Associates liquid chromatograph. Two RCM-100 radial compression modules were used in series. The pigments were eluted using a linear gradient from 90:10 acetonitrile:water to 100% ethyl acetate over 20 minutes at 2mL/min. They were detected using a Waters 440 absorbance detector and integrated using a Waters Data Module. Small peak heights were measured manually and converted to pigment abundance using individual calibration curves, as the Data Module was found to be inaccurate when the signal-to-noise ratio was low. Absorption spectra were taken using a Hewlett-Packard 8450A spectrophotometer. Data are logically consistent, as oceanographic data (CTD measurements) were also obtained at the same sampling sites as the phytoplankton water collection sites. Large variations were found in the vertical distribution of the marker pigments along each north-south transect. In the major pigments, this represented patchiness in the distribution of phytoplankton in the water column, as the accuracy of the HPLC technique is within 5%. In minor pigments, experimental errors would have been more significant, and would have been superimposed on this patchiness. These data were manually typed in from ANARE Research Notes 58 by AADC staff. It is possible that some typing errors occurred. Several inconsitencies in the printed data were also discovered.
Hierarchy level
Dataset
Maintenance and update frequency
As needed
Maintenance note
2019-03-18 - record updated by Dave Connell for ISO compliance.

Metadata

Metadata identifier
string/SIBEX_II_phyto

Language
English
Character encoding
UTF8

Author

WRIGHT, SIMON
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Sponsor

Australian Antarctic Division

Owner

AADC

Type of resource

Resource scope
Dataset

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
51b14997-c5ed-4aec-9a3f-bf71e61ce4d6

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Date (Last Revision)
2019-03-18T09:35:14

Identifier

Description
metadata.extraction_date

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
8.6

Metadata linkage
http://data.aad.gov.au/metadata/records/SIBEX_II_phyto

Point of truth for the metadata record

Date info (Creation)
1999-10-07T00:00:00
Date info (Last Update)
2019-03-18

Metadata standard

Title
ISO 19115-3
Edition
2014
Other citation details
Version 1
Title
DIF to ISO 19115-1 Profile
 
 

Overviews

Spatial extent

N
S
E
W


Keywords


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