Molecular comparison of bacterial diversity in uncontaminated and hydrocarbon contaminated marine sediment

Sediment samples which were originally collected as part of ASAC 868 (ASAC_868) are now being investigated using molecular microbial techniques as part of ASAC 1228 (ASAC_1228). Samples were collected in a nested survey design in two hydrocarbon impacted areas and two unimpacted areas. Denaturing gradient gel electrophoresis (DGGE) of a region of the 16S RNA gene was used to investigate the microbial community structure. Banding patterns obtained from the DGGE were transformed into a presence / absence matrix and analysed with a multivariate statistical approach.


The download file contains an excel spreadsheet, a csv version of the data, plus a readme file.

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Identification info

Alternate title
Molecular comparison of bacterial diversity in uncontaminated and hydrocarbon contaminated marine sediment
Date (Publication)
2011-01-11
Edition
1
Citation identifier
Dataset DOI

Title
Information and documentation - Digital object identifier system
Date (Publication)
2012-04-23
Citation identifier
ISO 26324:2012

Citation identifier
doi:10.26179/5c776b13016c4

Originator

Bowman, J. and Powell, S.

Publisher

Australian Antarctic Data Centre

Principal investigator

BOWMAN, JOHN
School of Agricultural Science
University of Tasmania
Private Bag 54
Hobart
Tasmania
7001
Australia

Principal investigator

POWELL, SHANE
Tasmanian Institute of Agricultural Research
University of Tasmania
Private Bag 54
Hobart
Tasmania
7001
Australia

Collaborator

POWELL, SHANE
Tasmanian Institute of Agricultural Research
University of Tasmania
Private Bag 54
Hobart
Tasmania
7001
Australia
Name
CAASM Metadata
Website
https://data.aad.gov.au/metadata/records/Nested_DGGE

Status
Completed

Custodian

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Topic category
  • Biota
  • Environment
  • Oceans

Extent

N
S
E
W


Extent

Description
Temporal Coverage

Temporal extent

TimePeriod
1997-11-01 1998-11-30
Title
Long-Term Fate of Petroleum Contaminations at Casey Station, Antarctica.
Date (Publication)
1997

Author

Guille D., Revill A.T., Bowman J.
Page
79
Title
A comparison of the short term effects of diesel fuel and lubricant oils on Antarctic benthic microbial communities.
Date (Publication)
2005
Citation identifier
322

Author

Powell S.M., Snape I., Bowman J.P., Thompson B.A.W., Stark J.S., McCammon S.A., Riddle M.J.
Name
Journal of Experimental Marine Biology and Ecology
Issue identification
1
Page
53-65
Title
Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community.
Date (Publication)
2005
Citation identifier
17

Author

Powell S., Riddle M.J., Snape I., Stark J.S.
Name
Antarctic Science
Issue identification
3
Page
353-360
Title
Degradation of nonane by bacteria from Antarctic marine sediment.
Date (Publication)
2004
Citation identifier
27

Author

Powell S.M., Bowman J.P., Snape I.
Name
Polar Biology
Page
573-578
Title
Microbial community variation in pristine and polluted nearshore Antarctic sediments.
Date (Publication)
2003
Citation identifier
45

Author

Powell S.M., Bowman J.P., Snape I., Stark J.S.
Name
FEMS Microbiology Ecology
Page
135-145
Title
Antarctic microbial communities that degrade hydrocarbons at different temperatures.
Date (Publication)
2004

Author

Powell S.M., Ferguson S.H., Snape I., Bowman J.P., Franzmann P.D., Gibson J.A.E.
Name
Filler D.M., Barnes D.L. Abstract Proceedings of the 4th International Conference. Contaminants in Freezing Ground. Fairbanks, Alaska. 30 May - 3 June 2004
Page
22
Title
Microbial ecology of hydrocarbon - contaminated Antarctic sites.
Date (Publication)
2006

Author

Powell S.M., Bowman J.P., Ferguson S.H., Snape I., Stark J.S., Sicilano S.D., Riddle M.J.
Name
Abstracts of the SCAR Open Science Conference, Hobart, 12-14 July 2006
Title
Short term effects of four different oils on Antarctic benthic microbial communities.
Date (Publication)
2003

Author

Powell S.M., Thompson B., Stark J.S., Bowman J.P., Snape I., Riddle M.J.
Name
SETAC-ASE Asia-Pacific Conference, Christchurch 2003
NASA/GCMD Earth Science Keywords
  • EARTH SCIENCE > HUMAN DIMENSIONS > ENVIRONMENTAL IMPACTS > CONTAMINANT LEVELS/SPILLS
  • EARTH SCIENCE > HUMAN DIMENSIONS > ENVIRONMENTAL IMPACTS > OIL SPILLS
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA
  • EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > COMMUNITY DYNAMICS > COMMUNITY STRUCTURE
Keywords
  • BACTERIAL DIVERSITY
  • BACTERIAL COMMUNITY STRUCTURE
  • DENATURING GRADIENT GEL ELECTROPHORESIS
  • HYDROCARBONS
  • NON-METRIC MULTI DIMENSIONAL SCALING PLOTS
NASA/GCMD Earth Science Keywords
  • SEDIMENT CORERS
NASA/GCMD Earth Science Keywords
  • FIELD SURVEYS
NASA/GCMD Earth Science Keywords
  • AMD/AU
  • CEOS
  • AMD
NASA/GCMD Earth Science Keywords
  • CONTINENT > ANTARCTICA
  • CONTINENT > ANTARCTICA > Casey
  • CONTINENT > ANTARCTICA > Brown Bay
  • CONTINENT > ANTARCTICA > O'Brien Bay
  • CONTINENT > ANTARCTICA > Sparkes Bay
  • CONTINENT > ANTARCTICA > Newcomb Bay
  • GEOGRAPHIC REGION > POLAR

Resource constraints

Use limitation
This metadata record is publicly available.

Resource constraints

Access constraints
licence
Other constraints
These data are publicly available for download at the provided URL.

Resource constraints

File type
Portable Network Graphic
Linkage
Creative Commons by Attribution logo

Title
Attribution 4.0 International (CC BY 4.0)
Website
https://creativecommons.org/licenses/by/4.0/legalcode

Legal code for Creative Commons by Attribution 4.0 International license

Use constraints
licence
Other constraints
This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=Nested_DGGE when using these data.
Language
English
Character encoding
UTF8

Distribution Information

Distributor

Distributor

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Distributor

Fees
Free
Planned available datetime
2011-01-11T00:00:00
Units of distribution
kb
Transfer size
11
Distribution format
  • Excel, CSV, Text

OnLine resource
GET DATA

Download point for the data.

OnLine resource
PROJECT HOME PAGE

Public information for ASAC project 1228

OnLine resource
VIEW RELATED INFORMATION

Citation reference for this metadata record and dataset

Maintenance and update frequency
As needed
Maintenance note
2011-01-11 - record updated by Dave Connell after data were submitted by Shane Powell. 2019-02-28 - record updated by Dave Connell - basic updates.

Metadata

Metadata identifier
string/Nested_DGGE

Language
English
Character encoding
UTF8

Author

POWELL, SHANE
Tasmanian Institute of Agricultural Research
University of Tasmania
Private Bag 54
Hobart
Tasmania
7001
Australia

Sponsor

Australian Antarctic Division

Owner

AADC

Type of resource

Resource scope
Dataset

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
23aa063e-4589-4b04-82eb-97d68a4512b0

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Date (Last Revision)
2019-02-28T12:14:48

Identifier

Description
metadata.extraction_date

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
8.6

Metadata linkage
http://data.aad.gov.au/metadata/records/Nested_DGGE

Point of truth for the metadata record

Date info (Creation)
2002-01-03T00:00:00
Date info (Last Update)
2019-02-28

Metadata standard

Title
ISO 19115-3
Edition
2014
Other citation details
Version 1
Title
DIF to ISO 19115-1 Profile
 
 

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