Sampling of planktonic microorganisms for metagenomic, metaproteomic and metatranscriptomic analyses
Large volumes of water (200 - 500 L) were filtered and fractionated by size for various planktonic components: eukaryotic phytoplankton, prokaryotic picoplankton, and marine viruses. Sample sites were chosen to generate the widest diversity, and included planktonic blooms, oligotrophic zones, small polynyas near sea ice, nearshore areas, and Antarctic bottom water from coastal, canyon and deepwater areas. Half of each sample will be used for DNA library construction, and the other half will be used for meta-proteomic analysis. Random shotgun sequencing of the marine genomic libraries should produce a metagenomic snapshot of planktonic life in a variety of marine habitats.
This work was completed as part of ASAC project 2899 (ASAC_2899).
Simple
Identification info
- Alternate title
- Sampling of planktonic microorganisms for metagenomic, metaproteomic and metatranscriptomic analyses
- Date (Publication)
- 2012-04-24
- Edition
- 1
Originator
Publisher
Principal investigator
Collaborator
Collaborator
- Name
- CAASM Metadata
- Website
- https://data.aad.gov.au/metadata/records/CEAMARC_CASO_200708_V3_PLANKTON_GENOMICS_PROTEOMICS
- Status
- Completed
Custodian
- Topic category
-
- Biota
- Oceans
Extent
))
Extent
- Description
- Temporal Coverage
Temporal extent
- TimePeriod
- 2007-12-17 2008-01-28
- NASA/GCMD Earth Science Keywords
-
- EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
- Keywords
-
- phytoplankton
- NASA/GCMD Earth Science Keywords
-
- SHIPS
- R/V AA > R/V Aurora Australis
- NASA/GCMD Earth Science Keywords
-
- IPY > INTERNATIONAL POLAR YEAR
- CASO > Climate of Antarctica and the Southern Ocean
- NASA/GCMD Earth Science Keywords
-
- AMD/AU
- CEOS
- AMD
- NASA/GCMD Earth Science Keywords
-
- OCEAN > SOUTHERN OCEAN
- GEOGRAPHIC REGION > POLAR
Resource constraints
- Use limitation
- This metadata record is publicly available.
Resource constraints
- Access constraints
- licence
- Other constraints
- These data are publicly available from the provided URL.
Resource constraints
- File type
- Portable Network Graphic
- Title
- Attribution 4.0 International (CC BY 4.0)
- Website
-
https://creativecommons.org/licenses/by/4.0/legalcode
Legal code for Creative Commons by Attribution 4.0 International license
- Use constraints
- licence
- Other constraints
- This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=CEAMARC_CASO_200708_V3_PLANKTON_GENOMICS_PROTEOMICS when using these data.
- Language
- English
- Character encoding
- UTF8
Distribution Information
Distributor
Distributor
Distributor
- Fees
- free
- Planned available datetime
- 2012-04-24T00:00:00
- OnLine resource
-
GET DATA
Download point for the data - excel spreadsheet
- OnLine resource
-
GET DATA
Genomic data obtained during V3 (2008). This is the primary data portal for genomic metadata, and stands for Community cyber-infrastructure for the Advancement of Microbial Ecology Research and Analysis (CAMERA). All genomic data obtained during this cruise will be curated and posted for public consumption at this site, in addition to being deposited in the major public genomic databases.
- OnLine resource
-
GET DATA
Genbank/NCBI website
- OnLine resource
-
PROJECT HOME PAGE
Public information for ASAC project 2899
- OnLine resource
-
VIEW RELATED INFORMATION
Citation reference for this metadata record and dataset.
Resource lineage
- Statement
- No known quality issues. GOS stands for Global Ocean Sampling. TFF- Tangential Flow Filtration.
- Hierarchy level
- Dataset
- Maintenance and update frequency
- As needed
- Maintenance note
- 2008-02-25 - Preliminary metadata record created by Alicja Mosbauer. 2008-04-08 - Related URL fields slightly altered by Dave Connell 2008-05-20 - record updated by Dave Connell - link to excel spreadsheet added and link to ASAC project 2899 added. 2009-03-20 - record updated by Dave Connell to correct broken URL. 2009-09-11 - record updated by Dave Connell to include "Oceans" ISO Topic Category for inclusion in the IMOS Oceans Portal. 2012-04-24 - record updated by Dave Connell to add a link to Genbank at the request of Henk Brolsma. 2013-02-19 - record updated by Dave Connell. Minor corrections. 2017-09-06 - record updated by Dave Connell - basic updates.
Metadata
- Metadata identifier
- string/CEAMARC_CASO_200708_V3_PLANKTON_GENOMICS_PROTEOMICS
- Language
- English
- Character encoding
- UTF8
Author
Sponsor
Owner
Type of resource
- Resource scope
- Dataset
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Citation identifier
- ee6b8c30-ddac-4d73-ae31-929e75b44679
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Date (Last Revision)
- 2015-11-29T19:39:48
Identifier
- Description
- metadata.extraction_date
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Citation identifier
- 8.6
- Metadata linkage
-
http://data.aad.gov.au/metadata/records/CEAMARC_CASO_200708_V3_PLANKTON_GENOMICS_PROTEOMICS
Point of truth for the metadata record
- Date info (Creation)
- 2008-02-24T00:00:00
- Date info (Last Update)
- 2017-09-06
Metadata standard
- Title
- ISO 19115-3
- Edition
- 2014
- Other citation details
- Version 1
- Title
- DIF to ISO 19115-1 Profile
Overviews
Spatial extent
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