TemperateReefBase Geonetwork Catalogue
BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006
This data set contains concentrations of phytoplankton, protozoa, total bacteria and metabolically active bacteria assessed by flow cytometry on transects 12, 1, 3, 5, 7, 9 and 11 of the BROKE-West survey of the Southern Ocean between January and March 2006. Only total bacterial concentrations were assessed for transect 11.
Between 4 and 12 depths were sampled for marine microbes and concentrations were assessed using FACScan flowcytometer. Phytoplankton were identified and counted based on the autofluorescense of chlorophyll a when excited by the 488 nm laser of the FACScan. Protozoa were identified and counted after staining with the acid vacuole stain Lysotracker Green. Total bacteria were identified and counted using the cell permeant SYTO 13 nucleic stain. Metabolically active bacteria were identified and counted after staining for intracellular esterases with the esterase stain 6CFDA.
The fields in this dataset are:
Latitude
Longitude
Transect Number
CTD number, flow file
Depth (m)
Total bacteria (per millilitre)
Active bacteria (per millilitre)
Dead bacteria (per millilitre)
Protozoa (per millilitre)
Phytoplankton (per millilitre)
This work was completed as part of ASAC project 40 (ASAC_40).
Simple
Identification info
- Alternate title
- BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006
- Date (Publication)
- 2010-08-20
- Edition
- 1
- Citation identifier
-
Dataset DOI
- Title
- Information and documentation - Digital object identifier system
- Date (Publication)
- 2012-04-23
- Citation identifier
- ISO 26324:2012
- Citation identifier
- doi:10.4225/15/59890669dda1a
Originator
Publisher
Principal investigator
Collaborator
- Name
- CAASM Metadata
- Status
- Completed
Custodian
- Topic category
-
- Biota
- Oceans
Extent
))
Extent
- Description
- Temporal Coverage
Temporal extent
- TimePeriod
- 2006-01-10 2006-02-27
Extent
- Description
- Vertical Extent - Depth
Vertical element
- Minimum value
- 5
- Maximum value
- 150
- Identifier
- urn:x-ogc:def:cs:EPSG:6498
- Name
- Vertical CS. Axis: depth (D). Orientation: down. UoM: m.
- Identifier
- urn:x-ogc:def:axis:EPSG:113
- Name
- depth
- NASA/GCMD Earth Science Keywords
-
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA
- EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
- EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > PELAGIC
- Keywords
-
- BROKE-West
- Flow cytometry
- bacteria
- FACScan Flow Cytometer
- Latitude
- Longitude
- Transect Number
- CTD
- Depth
- Total bacteria
- Active bacteria
- Dead bacteria
- Protozoa
- Phytoplankton
- NASA/GCMD Earth Science Keywords
-
- FLOW CYTOMETRY
- NASA/GCMD Earth Science Keywords
-
- SHIPS
- R/V AA > R/V Aurora Australis
- NASA/GCMD Earth Science Keywords
-
- AMD/AU
- CEOS
- AMD
- ACE/CRC
- NASA/GCMD Earth Science Keywords
-
- OCEAN > SOUTHERN OCEAN
- CONTINENT > ANTARCTICA
- GEOGRAPHIC REGION > POLAR
Resource constraints
- Use limitation
- This metadata record is publicly available.
Resource constraints
- Access constraints
- licence
- Other constraints
- These data are publicly available for download from the provided URL.
Resource constraints
- File type
- Portable Network Graphic
- Title
- Attribution 4.0 International (CC BY 4.0)
- Website
-
https://creativecommons.org/licenses/by/4.0/legalcode
Legal code for Creative Commons by Attribution 4.0 International license
- Use constraints
- licence
- Other constraints
- This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=BROKE-West_flow_cytometry when using these data.
- Language
- English
- Character encoding
- UTF8
Distribution Information
Distributor
Distributor
Distributor
- Fees
- free
- Planned available datetime
- 2010-08-20T00:00:00
- Units of distribution
- kb
- Transfer size
- 10
- Distribution format
-
- csv
- OnLine resource
-
GET DATA
Download point for the data - csv spreadsheet
- OnLine resource
-
PROJECT HOME PAGE
Download point for the data - CTD Bottle Water Data Index page
- OnLine resource
-
PROJECT HOME PAGE
Public information for ASAC project 40
- OnLine resource
-
VIEW RELATED INFORMATION
Citation reference for this metadata record and dataset
- Maintenance and update frequency
- As needed
- Maintenance note
- 2010-08-20 - record updated by Dave Connell to publicly release the data. 2017-08-08 - record updated by Dave Connell - basic updates.
Metadata
- Metadata identifier
- string/BROKE-West_flow_cytometry
- Language
- English
- Character encoding
- UTF8
Author
Sponsor
Owner
- Title
- Parent Metadata Record
- Citation identifier
- BROKE-West
Type of resource
- Resource scope
- Dataset
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Citation identifier
- f9477397-0e4d-46bf-b9ef-b514e34df3d9
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Date (Last Revision)
- 2015-11-30T07:08:23
Identifier
- Description
- metadata.extraction_date
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Citation identifier
- 8.6
- Metadata linkage
-
http://data.aad.gov.au/metadata/records/BROKE-West_flow_cytometry
Point of truth for the metadata record
- Date info (Creation)
- 2006-05-19T00:00:00
- Date info (Last Update)
- 2017-08-08
Metadata standard
- Title
- ISO 19115-3
- Edition
- 2014
- Other citation details
- Version 1
- Title
- DIF to ISO 19115-1 Profile
Overviews
Spatial extent
Provided by
