TemperateReefBase Geonetwork Catalogue
Identity, composition and abundance of protists - data collected from the BROKE-West voyage of the Aurora Australis, 2006
The BROKE-West survey was conducted from 30 degrees E and 80 degrees E between January and March 2006. It consisted of 1 east-west transect at the northernmost limit of the survey between 60 degrees S and 62 degrees S between the 10 and 19 of January, followed by 11 meridional transects separated by 5 degrees of longitude and extending from approximately 62 degrees S to the Antarctic continental shelf between the 19 January and 3 March.
This dataset details research undertaken to determine the identity, composition and abundance of protists in the survey area.
Some explanations for terms used in the dataset are as follows:
1. Group is the taxonomic Phylum, Class and occasionally Order to which the taxa belongs. The abbreviations are:
a. Diatom - Diatomophyceae/Bacillariophyceae. These are subdivided into the taxonomic Orders Centrales (centric) and Pennales (pennate)
b. Chryso - Chrysophyceae
c. Dino - Dinophyceae
d. Eugleno - Euglenophyceae
e. Hapto - Haptophyceae
f. Prasino - Prasinophyceae
g. Silico - Dictyochales (silicoflagellates)
h. Choano - Craspedophyceae /Choanoflagellida
i. Ciliate - Phylum Ciliophora
j. Tintinnid - Phylum Ciliophora , Order Spirotrichea, Class Tintinnida
k. Protozoa - refers to grouped rare taxa belonging to a number of Classes
2. Autotroph/Heterotroph refers to the trophic status of the taxa, indicating whether they are autotrophic (plant) or heterotrophic (animal).
3. CTD Station Number refers to the station at which samples were collected. Type indicates whether the samples were collected at the surface "surf" or at the point at which there was maximum chlorophyll fluorescence detected from the CTD flurometer trace "ChlMax".
The data are all cell concentration numbers.
For more information, see the other metadata records related to ASAC project 40 (ASAC_40), ASAC project 2655 (ASAC_2655) and ASAC project 2679 (ASAC_2679).
Simple
Identification info
- Alternate title
- Identity, composition and abundance of protists - data collected from the BROKE-West voyage of the Aurora Australis, 2006
- Date (Publication)
- 2010-08-20
- Edition
- 1
- Citation identifier
-
Dataset DOI
- Title
- Information and documentation - Digital object identifier system
- Date (Publication)
- 2012-04-23
- Citation identifier
- ISO 26324:2012
- Citation identifier
- doi:10.4225/15/598d40bd3adda
Originator
Publisher
Principal investigator
Collaborator
Collaborator
Collaborator
Collaborator
- Name
- CAASM Metadata
- Status
- Completed
Custodian
- Topic category
-
- Biota
- Oceans
Extent
))
Extent
- Description
- Temporal Coverage
Temporal extent
- TimePeriod
- 2006-01-10 2006-02-27
Extent
- Description
- Vertical Extent - Depth
Vertical element
- Minimum value
- 4.6
- Maximum value
- 102
- Identifier
- urn:x-ogc:def:cs:EPSG:6498
- Name
- Vertical CS. Axis: depth (D). Orientation: down. UoM: m.
- Identifier
- urn:x-ogc:def:axis:EPSG:113
- Name
- depth
- NASA/GCMD Earth Science Keywords
-
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS
- EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CHLOROPHYLL
- EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PIGMENTS > CHLOROPHYLL
- Keywords
-
- Protists
- Autotrophs
- Heterotrophs
- BROKE-West
- chlorophyll
- CTD
- NASA/GCMD Earth Science Keywords
-
- CTD > Conductivity, Temperature, Depth
- NISKIN BOTTLES
- SCANNING ELECTRON MICROSCOPES
- TEM > TRANSMISSION ELECTRON MICROSCOPY
- NASA/GCMD Earth Science Keywords
-
- R/V AA > R/V Aurora Australis
- SHIPS
- NASA/GCMD Earth Science Keywords
-
- AMD/AU
- CEOS
- AMD
- ACE/CRC
- NASA/GCMD Earth Science Keywords
-
- CONTINENT > ANTARCTICA
- OCEAN > SOUTHERN OCEAN
- GEOGRAPHIC REGION > POLAR
Resource constraints
- Use limitation
- This metadata record is publicly available.
Resource constraints
- Access constraints
- licence
- Other constraints
- These data are publicly available for download at the provided URL.
Resource constraints
- File type
- Portable Network Graphic
- Title
- Attribution 4.0 International (CC BY 4.0)
- Website
-
https://creativecommons.org/licenses/by/4.0/legalcode
Legal code for Creative Commons by Attribution 4.0 International license
- Use constraints
- licence
- Other constraints
- This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=BROKE-West_Protists when using these data.
- Language
- English
- Character encoding
- UTF8
Distribution Information
Distributor
Distributor
Distributor
- Fees
- free
- Planned available datetime
- 2010-08-20T00:00:00
- Units of distribution
- kb
- Transfer size
- 21
- Distribution format
-
- Excel
- OnLine resource
-
GET DATA
Download point for the data
- OnLine resource
-
PROJECT HOME PAGE
Public information for ASAC project 40
- OnLine resource
-
PROJECT HOME PAGE
Public information for ASAC project 2655
- OnLine resource
-
PROJECT HOME PAGE
Public information for ASAC project 2679
- OnLine resource
-
VIEW RELATED INFORMATION
Citation reference for this metadata record and dataset
Resource lineage
- Statement
- Sea water samples for determining the identity, composition and abundance of protists were obtained using 20 l Niskin bottles (General Oceanics) mounted on a CTD rosette at 30 sites in surface waters (3-12 m depth) and 26 sites at the Chl max (11-80 m) over a total of 34 locations during the BROKE-West survey. Samples were preserved with 1% vol:vol Lugol's iodine and stored in glass bottles, in the dark at 4 degrees C until processing for either light microscopy or electron microscopy. Permanent slides were prepared according to the 2-hydroxypropyl methacrylate (HPMA) (Sigma) method of Crumpton (1981), further modified by Sung-Ho Kang (KORDI, pers. comm.). Each sample was filtered at less than 5 kPa onto a 0.45 micron pore size, 25 mm diameter, GN-6 cellulosic membrane filter (Gelman). The filter was then rinsed by filtering a further 15 ml of MilliQ water, removed from the filtration apparatus and placed face down on a cover glass. A few drops of HPMA were placed on the back of the filter and the sample was then transferred to a 60 degrees C cabinet for 12 to 24 h to clear the filter and polymerise the HPMA. Further drops of HPMA were then placed on the back of the filter, a slide placed on top and the sample again polymerised as above for 6-12 h. Identification of cryptic species was aided by electron microscopy. One litre samples were fixed with 1% glutaraldehyde and stored at 4 degrees C until processed. Each sample was concentrated over a 47 mm diameter 0.8 microns polycarbonate membrane (Poretics) at a vacuum of less than 50 mm Hg and the concentrate resuspended into the sea water remaining above the filter by gentle agitation. For scanning electron microscopy (SEM) cells were settled onto a polylysine-coated glass coverslips, dehydrated with a graded series of acetone, critical point dried, sputter coated with gold and examined using a JEOL JSM 840 SEM. For transmission microscopy (TEM), approximately 40 microlitre aliquots of concentrate were placed on polylysine treated formvar-coated copper grids (Marchant and Thomas, 1983) and fixed for 60 s with 2% OsO4 vapour. The aliquot was allowed to evaporate and the grid was gently rinsed with distilled water. The grids were either negative-stained with 2% uranyl acetate for 30 sec or shadow-cast with platinum/palladium or chromium and examined with a Phillips CM 100 TEM. Prepared slides were examined using a Zeiss Axioskop microscope equipped with Nomarski interference optics at 400x magnification. Mean concentrations of each protistan taxa were calculated from the number of cells observed over 20 randomly selected quadrats (Whipple grids) for each sample.
- Hierarchy level
- Dataset
- Maintenance and update frequency
- As needed
- Maintenance note
- 2009-08-25 - record created by Dave Connell from a word document submitted by Andrew Davidson. 2010-08-20 - record updated by Dave Connell to publicly release the data. 2012-06-21 - record updated by Dave Connell to add a parent DIF. 2017-08-11 - record updated by Dave Connell - basic updates.
Metadata
- Metadata identifier
- string/BROKE-West_Protists
- Language
- English
- Character encoding
- UTF8
Author
Sponsor
Owner
- Title
- Parent Metadata Record
- Citation identifier
- BROKE-West
Type of resource
- Resource scope
- Dataset
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Citation identifier
- 923eb4e8-e37d-48ed-847a-0e5fbeeafe78
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Date (Last Revision)
- 2015-11-30T07:08:45
Identifier
- Description
- metadata.extraction_date
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Citation identifier
- 8.6
- Metadata linkage
-
http://data.aad.gov.au/metadata/records/BROKE-West_Protists
Point of truth for the metadata record
- Date info (Creation)
- 2009-08-25T00:00:00
- Date info (Last Update)
- 2017-08-11
Metadata standard
- Title
- ISO 19115-3
- Edition
- 2014
- Other citation details
- Version 1
- Title
- DIF to ISO 19115-1 Profile
Overviews
Spatial extent
Provided by
