Molecular Characterisation of Cold-Adapted Metallo-Oxotransferases from the Dimethylsulfoxide Reductase Family Expressed by Antarctic Bacteria
Metadata record for data from ASAC Project 2547
See the link below for public details on this project.
Pue (greater than 90% as determined by SDS-PAGE) samples of nitrate reductase have been isolated from the Antarctic bacterium, Shewanella gelidimarina (ACAM 456T; Accession number U85907 (16S rDNA)). The protein is ~90 kDa (similar to nitrate reductase enzymes characterised from alternate bacteria) and stains positive in an in-situ nitrate reduction (native) assay technique. The protein may be N-terminal blocked, although further sequencing experiments are required to confirm this.
This work is based upon phenotyped Antarctic bacteria (S. gelidimarina; S.frigidimarina) that was collected during other ASAC projects. (Refer: Psychrophilic Bacteria from Antarctic Sea-ice and Phospholipids of Antarctic sea ice algal communities new sources of PUFA [ASAC_708] and Biodiversity and ecophysiology of Antarctic sea-ice bacteria [ASAC_1012]).
The download file contains 4 scientific papers produced from this work - one of these papers also contains a large set of accession numbers for data stored at GenBank.
Simple
Identification info
- Alternate title
- Molecular Characterisation of Cold-Adapted Metallo-Oxotransferases from the Dimethylsulfoxide Reductase Family Expressed by Antarctic Bacteria
- Date (Publication)
- 2005-03-02
- Edition
- 1
Originator
Publisher
Principal investigator
Collaborator
- Name
- CAASM Metadata
- Other citation details
- Restricted access
- Status
- Completed
Custodian
- Topic category
-
- Biota
- Oceans
Extent
Extent
- Description
- Temporal Coverage
Temporal extent
- TimePeriod
- 2004-07-01 2005-07-31
Extent
- Description
- Vertical Extent - Altitude
Vertical element
- Minimum value
- 0
- Maximum value
- 100
- Identifier
- urn:x-ogc:def:cs:EPSG:6499
- Name
- Vertical CS. Axis: height (H). Orientation: up. UoM: m.
- Identifier
- urn:x-ogc:def:axis:EPSG:114
- Name
- height
Extent
- Description
- Vertical Extent - Depth
Vertical element
- Minimum value
- 0
- Maximum value
- 10
- Identifier
- urn:x-ogc:def:cs:EPSG:6498
- Name
- Vertical CS. Axis: depth (D). Orientation: down. UoM: m.
- Identifier
- urn:x-ogc:def:axis:EPSG:113
- Name
- depth
- Title
- Life on Earth. Extremophiles Continue to Move the Goal Posts
- Date (Publication)
- 2006
- Citation identifier
- 3
Author
- Name
- Environ. Chem.
- Page
- 77-93
- Title
- Studies of Iron-Uptake Mechanisms in Two Bacterial Species of the Shewanella Genus Adapted to Middle-Range (Shewanella putrefaciens) or Antarctic (Shewanella gelidimarina) Temperatures
- Date (Publication)
- 2008
- Citation identifier
- 5
Author
- Name
- Chemistry and Biodiversity
- Page
- 2113-2123
- Title
- The periplasmic nitrate reductase in Shewanella: the resolution, distribution and functional implications of two NAP isoforms, NapEDABC and NapDAGHB
- Date (Publication)
- 2010
- Citation identifier
- 156
Author
- Name
- Microbiology
- Page
- 302-312
- Title
- Resolution of two native monomeric 90 kDa nitrate reductase active proteins from Shewanella gelidimarina and the sequence of two napA genes
- Date (Publication)
- 2010
- Citation identifier
- 398
Author
- Name
- Biochemical and Biophysical research Communications
- Page
- 13-18
- NASA/GCMD Earth Science Keywords
-
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA
- Keywords
-
- ACAM
- BACTERIA
- NASA/GCMD Earth Science Keywords
-
- MICROSCOPES
- NASA/GCMD Earth Science Keywords
-
- LABORATORY
- NASA/GCMD Earth Science Keywords
-
- AMD/AU
- AMD
- CEOS
- NASA/GCMD Earth Science Keywords
-
- CONTINENT > ANTARCTICA
- GEOGRAPHIC REGION > POLAR
Resource constraints
- Use limitation
- This metadata record is publicly available.
Resource constraints
- Access constraints
- licence
- Other constraints
- Copies of the publications, plus a list of accession numbers are available for download from the provided URL to AAD staff only.
Resource constraints
- File type
- Portable Network Graphic
- Title
- Attribution 4.0 International (CC BY 4.0)
- Website
-
https://creativecommons.org/licenses/by/4.0/legalcode
Legal code for Creative Commons by Attribution 4.0 International license
- Use constraints
- licence
- Other constraints
- This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_2547 when using these data.
- Language
- English
- Character encoding
- UTF8
Distribution Information
Distributor
Distributor
Distributor
- Fees
- Free
- Units of distribution
- kb
- Transfer size
- 980
- Distribution format
-
- OnLine resource
-
GET DATA
Download point for the papers, plus a list of accession numbers - AAD Staff Only
- OnLine resource
-
GET DATA
GenBank home page
- OnLine resource
-
PROJECT HOME PAGE
Public information for ASAC project 2547
- OnLine resource
-
VIEW RELATED INFORMATION
Citation reference for this metadata record and dataset
Resource lineage
- Statement
- The latitudes and longitudes provided in spatial coverage are taken from the metadata records for ASAC projects 708 and 1012.
- Hierarchy level
- Dataset
- Maintenance and update frequency
- As needed
- Maintenance note
- 2011-02-18 - record updated by Dave Connell to link papers provided by Rachel Codd. 2016-04-29 - record updates by Dave Connell - basic updates. 2019-04-04 - record updated by Dave Connell for ISO compliance.
Metadata
- Metadata identifier
- string/ASAC_2547
- Language
- English
- Character encoding
- UTF8
Author
Sponsor
Owner
Type of resource
- Resource scope
- Dataset
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Citation identifier
- ae7e4d73-d044-488a-91e0-e5dd434c5bba
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Date (Last Revision)
- 2019-04-04T12:00:48
Identifier
- Description
- metadata.extraction_date
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Citation identifier
- 8.6
- Metadata linkage
-
http://data.aad.gov.au/metadata/records/ASAC_2547
Point of truth for the metadata record
- Date info (Creation)
- 2005-03-02T00:00:00
- Date info (Last Update)
- 2019-04-04
Metadata standard
- Title
- ISO 19115-3
- Edition
- 2014
- Other citation details
- Version 1
- Title
- DIF to ISO 19115-1 Profile