Antifreeze molecules in Nototheniid fish around Davis Station, Antarctica

A report completed as part of this project is available for download from the URL given below. Extracts of the report are presented in the metadata record.


See the report for full details.


Several species of Antarctic fish were collected from the shallow waters off Davis Station during the 2000-01 season as part of a study examining the properties of 'antifreeze' proteins contained within the blood of these animals. Fish were sampled at regular intervals from a range of depths and various sites near the station.


The main objectives of the study were to collect serum and selected tissues from Nototheniid (cod) and Channichthyid (ice fish) species. Over 170 fish were collected throughout the calendar year. Samples were taken as required, processed and the fish preserved for further analysis on return to Australia. In Australia the serum will be tested for special antifreeze molecules that allow these animals to live in water that is colder than the usual freezing point of their body fluids. Such molecules, once identified, may be synthesised in a laboratory, and have numerous potential practical applications, from the preservation of frozen foods, to preservation of blood plasma and organs for human transplant. Analyses of this nature will be undertaken at the University of Sydney.

Simple

Identification info

Alternate title
Antifreeze molecules in Nototheniid fish around Davis Station, Antarctica
Date (Publication)
2007-11-13
Edition
1

Originator

Haymet, T. and Williams, D.

Publisher

Australian Antarctic Data Centre

Principal investigator

HAYMET, TONY
University of Houston
Houston
Texas
USA

Principal investigator

WILLIAMS, DICK
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Collaborator

WILLIAMS, DICK
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Name
CAASM Metadata
Website
https://data.aad.gov.au/metadata/records/ASAC_2050

Status
Completed

Custodian

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Topic category
  • Biota
  • Oceans

Extent

N
S
E
W


Extent

Description
Temporal Coverage

Temporal extent

TimePeriod
2000-01-01 2001-12-31
Title
Valine substituted winter flounder 'antifreeze': preservation of ice growth hysteresis.
Date (Publication)
1998
Citation identifier
430

Author

Haymet A.D.J., Ward L.G., Harding M.M., Knight C.A.
Name
FEBS Letters
Page
301-306
Title
Winter flounder 'antifreeze' proteins: Synthesis and ice growth inhibition of analogues that probe the relative importance of hydrophobic and hydrogen bonding interactions.
Date (Publication)
1999
Citation identifier
121

Author

Haymet A.D.J., Ward L.G., Harding M.M.
Name
Allen J.Bard Journal of the American Chemical Society
Page
941-948
Title
Type 1 alpha-helical fish antifreeze proteins
Date (Publication)
1999
Citation identifier
264

Author

Ward L.G., Harding M.M., Haymet A.D.J.
Name
European Journal of Biochemistry
Page
653-665
Other citation details
Edited by Professor Richard Perham
Title
Why Polar Fish don't Freeze
Date (Publication)
1999
Citation identifier
66

Author

Harding M.M., Ward L.G., Haymet A.D.J.
Name
Chemistry in Australia
Page
31-33
NASA/GCMD Earth Science Keywords
  • EARTH SCIENCE > OCEANS > SEA ICE > ICE DEPTH/THICKNESS
  • EARTH SCIENCE > OCEANS > SEA ICE > PACK ICE
  • EARTH SCIENCE > OCEANS > SEA ICE
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH
  • EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > COASTAL
  • EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > PELAGIC
  • EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS
Keywords
  • Fish
  • Nototheniid fish
  • antifreeze
  • fishing
NASA/GCMD Earth Science Keywords
  • FIELD SURVEYS
  • SHIPS
  • LABORATORY
  • FIELD INVESTIGATION
NASA/GCMD Earth Science Keywords
  • AMD/AU
  • CEOS
  • AMD
NASA/GCMD Earth Science Keywords
  • CONTINENT > ANTARCTICA > Davis
  • GEOGRAPHIC REGION > POLAR

Resource constraints

Use limitation
This metadata record is publicly available.

Resource constraints

Access constraints
licence
Other constraints
A PDF copy of the report is available for download from the provided URL. The report also contains several tables of data.

Resource constraints

File type
Portable Network Graphic
Linkage
Creative Commons by Attribution logo

Title
Attribution 4.0 International (CC BY 4.0)
Website
https://creativecommons.org/licenses/by/4.0/legalcode

Legal code for Creative Commons by Attribution 4.0 International license

Use constraints
licence
Other constraints
This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_2050 when using these data.
Language
English
Character encoding
UTF8

Distribution Information

Distributor

Distributor

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Distributor

Fees
free
Planned available datetime
2007-11-13T00:00:00
Units of distribution
MB
Transfer size
3.3
Distribution format
  • pdf

OnLine resource
GET DATA

Download point for the data - PDF copy of the report

OnLine resource
PROJECT HOME PAGE

Public information for ASAC project 2050

OnLine resource
VIEW RELATED INFORMATION

Citation reference for this metadata record and dataset

Resource lineage

Statement
During the summer season, fishing was carried out on a regular basis (several days per week) principally from inflatable rubber boats (IRBs) using rods, handlines and/or fish traps. Fishing was also undertaken from the shoreline. Fishing from the shore proved to be most successful when traps were deployed. Casting from the shore, more often than not, resulted in lines becoming snagged on rocks and macroalgae. During winter, nearshore fishing sites were accessed by travel on foot or by skis. Traps were deployed through tide cracks or drilled holes. Fishing holes were drilled with a manual ice corer or a petrol powered jiffy drill. Fishtraps were used as the main means of collecting fish during winter as it was often too cold to sit outside for long periods with a rod and line. Blood samples were obtained from the caudal vein of the fish collected. Samples were taken in the field where possible. Blood samples were then transferred from the syringe to an eppendorf tube and stored overnight in a refrigerator to stimulate the red blood cells to settle out and clot. On the following day, tubes were transferred to a centrifuge and spun at 10,000 revolutions/sec for 4 to 5 minutes. The serum was then removed, transferred to a cryovial and stored in a -84 degrees C freezer. Muscle tissue samples were also taken. Small samples were removed from the anterior section of the tail. Tissue was excised between the lateral lines of the fish. Tissue was then transferred to a cryovial and stored in a -84 degrees C freezer. Both blood sera and muscle tissue samples were later transferred to a dewar of liquid nitrogen for transport back to Australia. Body mass, total body length and measures of snout-vent length were also obtained as were the location and depth of the fished site. All fish were then preserved in 10% formalin for a period of approximately 2 weeks and later transferred to 70% ethanol.
Hierarchy level
Dataset
Maintenance and update frequency
As needed
Maintenance note
2007-11-13 - record updated by Dave Connell - data report added. 2015-09-08 - record updated by Dave Connell. Basic updates.

Metadata

Metadata identifier
string/ASAC_2050

Language
English
Character encoding
UTF8

Author

CONNELL, DAVE J.
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Sponsor

Australian Antarctic Division

Owner

AADC

Type of resource

Resource scope
Dataset

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
9d686e58-ddec-4e42-9c6a-8bda4bde7b5e

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Date (Last Revision)
2015-11-29T18:22:04

Identifier

Description
metadata.extraction_date

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
8.6

Metadata linkage
http://data.aad.gov.au/metadata/records/ASAC_2050

Point of truth for the metadata record

Date info (Creation)
1999-10-07T00:00:00
Date info (Last Update)
2017-04-26

Metadata standard

Title
ISO 19115-3
Edition
2014
Other citation details
Version 1
Title
DIF to ISO 19115-1 Profile
 
 

Overviews

Spatial extent

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