TemperateReefBase Geonetwork Catalogue
Voyage 4 of the Aurora Australis, 2018 environmental DNA samples analysed with group specific metabarcoding markers
In March 2018, 23 environmental DNA (eDNA) samples (2 L of filtered seawater) were collected between Hobart, Tasmania and subantarctic Macquarie Island. These samples were processed using six different genetic metabarcoding markers targeting different taxonomic groups within the metazoan clade: A broad cytochrome c oxidase subunit I (COI) marker targeting all metazoans, and five different 16S markers targeting fish, cephalopods and crustaceans (one degenerate marker), fish (two markers of different lengths), cephalopods (one marker) and crustaceans (one marker). The aim of this study was to identify an ideal set of molecular markers to identify as many metazoan species as possible from small environmental samples, with a particular focus on vertebrates, crustaceans and cephalopods.
The data and methods are described in the word file "V4 2018 eDNA group specific markers.docx", results are summarised in the excel file "Marker.detection.xlsx" and additional sample information is in the excel files "2018_11_07_eDNA-sample-info.xls" and "sample.map.csv". Each genetic marker used in this study has its own folder, containing the raw FASTQ sequencing data, the processed FASTA sequencing data, the bioinformatics processing pipeline, the zOTU fasta file, BLAST output, MEGAN output and curated zOTU table. For further explanations please refer to the word file "V4 2018 eDNA group specific markers.docx".
Simple
Identification info
- Alternate title
- Voyage 4 of the Aurora Australis, 2018 environmental DNA samples analysed with group specific metabarcoding markers
- Date (Publication)
- 2023-02-28
- Edition
- 1
- Citation identifier
-
Dataset DOI
- Title
- Information and documentation - Digital object identifier system
- Date (Publication)
- 2012-04-23
- Citation identifier
- ISO 26324:2012
- Citation identifier
- doi:10.26179/0byh-s246
Originator
Publisher
Principal investigator
Principal investigator
Principal investigator
Collaborator
- Name
- CAASM Metadata
- Other citation details
- Unreleased data
- Purpose
- The aim of this study was to identify an ideal set of molecular markers to identify as many metazoan species as possible from small environmental samples, with a particular focus on vertebrates, crustaceans and cephalopods.
- Status
- Completed
Custodian
- Topic category
-
- Biota
- Oceans
Extent
))
Extent
- Description
- Temporal Coverage
Temporal extent
- TimePeriod
- 2018-03-09 2018-03-23
- NASA/GCMD Earth Science Keywords
-
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS
- Keywords
-
- PCR
- MISEQ
- ENVIRONMENTAL DNA
- METABARCODING
- BIODIVERSITY MONITORING
- NASA/GCMD Earth Science Keywords
-
- ADS > Automated DNA Sequencer
- NASA/GCMD Earth Science Keywords
-
- R/V AA > R/V Aurora Australis
- LABORATORY
- NASA/GCMD Earth Science Keywords
-
- AMD/AU
- AMD
- CEOS
- NASA/GCMD Earth Science Keywords
-
- OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND
- OCEAN > SOUTHERN OCEAN
- GEOGRAPHIC REGION > POLAR
Resource constraints
- Use limitation
- This metadata record is publicly available.
Resource constraints
- Access constraints
- licence
- Other constraints
- These data are not yet publicly available for download.
Resource constraints
- File type
- Portable Network Graphic
- Title
- Attribution 4.0 International (CC BY 4.0)
- Website
-
https://creativecommons.org/licenses/by/4.0/legalcode
Legal code for Creative Commons by Attribution 4.0 International license
- Use constraints
- licence
- Other constraints
- This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_group_specific_eDNA_V4_2018 when using these data.
- Language
- English
- Character encoding
- UTF8
Distribution Information
Distributor
Distributor
Distributor
- Fees
- Free
- Planned available datetime
- 2023-02-28T00:00:00
- Units of distribution
- GB
- Transfer size
- 7.24
- Distribution format
-
- FASTQ, Word, Excel, Text, csv
- OnLine resource
-
GET DATA > DIRECT DOWNLOAD
Download the dataset.
- OnLine resource
-
PROJECT HOME PAGE
Public information for AAS project AAS_4556
- OnLine resource
-
VIEW RELATED INFORMATION
Citation reference for this metadata record and dataset.
- Maintenance and update frequency
- As needed
- Maintenance note
- 2021-02-03 - record created by Leonie Suter.
Metadata
- Metadata identifier
- string/AAS_4556_group_specific_eDNA_V4_2018
- Language
- English
- Character encoding
- UTF8
Author
Sponsor
Owner
Type of resource
- Resource scope
- Dataset
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Citation identifier
- 3923f0b6-1de0-4b42-a3a2-d154a8cf33cc
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Date (Last Revision)
- 2021-02-04T11:31:54
Identifier
- Description
- metadata.extraction_date
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Citation identifier
- 8.6
- Metadata linkage
-
http://data.aad.gov.au/metadata/records/AAS_4556_group_specific_eDNA_V4_2018
Point of truth for the metadata record
- Date info (Creation)
- 2021-02-03T00:00:00
- Date info (Last Update)
- 2021-02-04
Metadata standard
- Title
- ISO 19115-3
- Edition
- 2014
- Other citation details
- Version 1
- Title
- DIF to ISO 19115-1 Profile
Overviews
Spatial extent
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