BAS Adelie Penguin Diet

June 2018

Adélie penguin scats were collected from Signy Island (South Orkney Islands) during crèche (December/January) 2014/15 and 2015/16 and stored in 80% Ethanol. DNA was extracted from ~30 mg of faecal material using a Promega ‘Maxwell 16' instrument and a Maxwell® 16 Tissue DNA kit.

A total of 450 samples were analysed: 30 extractions per week for 2015 and 60 per week for samples collected in 2016.


Three DNA markers providing different taxonomic information were amplified from penguin faecal DNA. First, ALL faecal DNA samples were characterised using a highly conserved metazoan primer set that amplifies a region of the nuclear 18S gene. In addition, a subset of faecal samples from each year were also characterised with two other primer pairs that amplify a region of the mtDNA 16S gene to allow species-level identification for most fish (16S_Fish) and krill (16S_Krill) species respectively. During amplification of markers, the products were tagged with a unique pair of index primers allowing samples to be pooled and sequenced (2x150bp) on a MiSeq high-throughput DNA sequencer.

- See Adelie Pengiun Diet CCAMLR paper for all of the primer/PCR details

- See BAS Adelie 18s Krill and Fish subset excel spreadsheet for sample details.

- See BAS Adelie 18s ALL samples fastq for 18s fastq files

- See BAS Adelie 16s Krill subset fastq for 16s krill fastq files

- See BAS Adelie Fish subset fastq for 16s fish fastq files

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November 2018

In addition we also amplified all 450 samples with the 16S_Fish marker.

- See Adelie Experiment Details 16s Fish for sample details, plate layout, first and second round PCR and miseq sheet.

- See BAS Adelie Fish ALL Samples fastq for 16s fish fastq files

Simple

Identification info

Alternate title
BAS Adelie Penguin Diet
Date (Publication)
2020-12-31
Edition
1
Citation identifier
Dataset DOI

Title
Information and documentation - Digital object identifier system
Date (Publication)
2012-04-23
Citation identifier
ISO 26324:2012

Citation identifier
doi:10.26179/5eb4e8619bf96

Originator

Deagle, B., Emmerson, L., McInnes, J.C., Polanowski, A., Waluda, C. and Dunn, M.

Publisher

Australian Antarctic Data Centre

Principal investigator

DEAGLE, BRUCE
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Principal investigator

EMMERSON, LOUISE
203 Channel Highway
Australian Antarctic Division
Kingston
Tasmania
7050
Australia

Principal investigator

MCINNES, JULIE C.
Australian Antarctic Division
203 Channel Highway
Institute for Marine and Antarctic Studies
University of Tasmania
Private Bag 129
Kingston
Tasmania
7050
Australia

Principal investigator

WALUDA, CLAIRE
British Antarctic Survey
High Cross, Madingley Road
CAMBRIDGE
CB3 0ET
United Kingdom

Principal investigator

DUNN, MICHAEL
British Antarctic Survey
High Cross, Madingley Road
CAMBRIDGE
CB3 0ET
United Kingdom

Collaborator

MCINNES, JULIE C.
Australian Antarctic Division
203 Channel Highway
Institute for Marine and Antarctic Studies
University of Tasmania
Private Bag 129
Kingston
Tasmania
7050
Australia

Collaborator

POLANOWSKI, ANDREA
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Name
CAASM Metadata
Website
https://data.aad.gov.au/metadata/records/AAS_4556_BAS_Adelie_Diet

Purpose
By monitoring the diets of key predators over a number of years, we can examine the relative availability of different prey items, as well as the particular characteristics of their foraging behaviour. Penguin diet variability is a key CEMP parameter and with the reduction in lavage sampling it is important to identify and assess alternative, less invasive methods as potential supplementary approaches for studying penguin diet. Diet analysis using DNA in faecal material is one alternative method which eliminates the need to handle the birds and is non-invasive.
Status
Completed

Custodian

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Topic category
  • Biota
  • Oceans

Extent

N
S
E
W


Extent

Description
Temporal Coverage

Temporal extent

TimePeriod
2014-12-29 2016-01-20
Title
Adelie penguin diet: a pilot study directly comparing data from stomach flushing with faecal DNA analysis.
Date (Publication)
2018

Author

B. Deagle, J McInnes, L. Emmerson, M.Dunn, S. Adlard and C. Waluda.

Publisher

CCAMLR
Hobart
Name
WG-EMM-18/29
NASA/GCMD Earth Science Keywords
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > BIRDS > PENGUINS
  • EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > USE/FEEDING HABITATS
  • EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > COMMUNITY DYNAMICS > INDICATOR SPECIES
Keywords
  • ADELIE PENGUIN
  • DNA DIET ANALYSIS
NASA/GCMD Earth Science Keywords
  • ADS > Automated DNA Sequencer
NASA/GCMD Earth Science Keywords
  • LABORATORY
  • FIELD INVESTIGATION
NASA/GCMD Earth Science Keywords
  • AMD
  • CEOS
  • AMD/AU
NASA/GCMD Earth Science Keywords
  • OCEAN > ATLANTIC OCEAN > SOUTH ATLANTIC OCEAN > SOUTH ORKNEY ISLANDS
  • GEOGRAPHIC REGION > POLAR
  • CONTINENT > ANTARCTICA > SIGNY ISLAND
  • OCEAN > SOUTHERN OCEAN

Resource constraints

Use limitation
This metadata record is publicly available.

Resource constraints

Access constraints
licence
Other constraints
These data are not yet publicly available for download.

Resource constraints

File type
Portable Network Graphic
Linkage
Creative Commons by Attribution logo

Title
Attribution 4.0 International (CC BY 4.0)
Website
https://creativecommons.org/licenses/by/4.0/legalcode

Legal code for Creative Commons by Attribution 4.0 International license

Use constraints
licence
Other constraints
This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_BAS_Adelie_Diet when using these data.
Language
English
Character encoding
UTF8

Distribution Information

Distributor

Distributor

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Distributor

Fees
Free
Planned available datetime
2020-12-31T00:00:00
Units of distribution
GB
Transfer size
11.1
Distribution format
  • fastq, Excel, PDF

OnLine resource
GET DATA > DIRECT DOWNLOAD

Download the dataset.

OnLine resource
PROJECT HOME PAGE

Public information for AAS project AAS_4556

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VIEW RELATED INFORMATION

Citation reference for this metadata record and dataset.

Maintenance and update frequency
As needed
Maintenance note
2020-05-07 - record created by Andrea Polanowski.

Metadata

Metadata identifier
string/AAS_4556_BAS_Adelie_Diet

Language
English
Character encoding
UTF8

Author

POLANOWSKI, ANDREA
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Sponsor

Australian Antarctic Division

Owner

AADC

Type of resource

Resource scope
Dataset

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
6f00b92f-13e0-409c-8f14-5ab94d852c75

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Date (Last Revision)
2020-05-21T11:47:58

Identifier

Description
metadata.extraction_date

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
8.6

Metadata linkage
http://data.aad.gov.au/metadata/records/AAS_4556_BAS_Adelie_Diet

Point of truth for the metadata record

Date info (Creation)
2020-05-07T00:00:00
Date info (Last Update)
2020-05-21

Metadata standard

Title
ISO 19115-3
Edition
2014
Other citation details
Version 1
Title
DIF to ISO 19115-1 Profile
 
 

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