TemperateReefBase Geonetwork Catalogue
Single metal toxicity to the Antarctic marine microalga Cryothecomonas armigera
This metadata record contains the results from 3 bioassays conducted with the Antarctic marine microalgae Cryothecomonas armigera (incertae sedis). These tests assessed the toxicity of copper, cadmium, lead, zinc and nickel.
Test conditions for both algae are described in the excel spreadsheets. In summary, tests for P. antarctica and C.armigera, were carried out at 0 plus or minus 2 degrees C, 20:4 h light:dark (60-90 micromol/m2/s, cool white 36W/840 globes), in 80 mL natural filtered (0.22 microns) seawater (salinity - 35 ppt, pH - 8.1 plus or minus 0.2). Filtered seawater was supplemented with 1.5 mg/L NO3- and 0.15 mg/L of PO43-. All tests were carried out in silanised 250-mL glass flasks, with glass lids.
Tests 1 and 2 consisted of metal treatments, with 3 replicates per treatment, alongside 3 replicate controls (natural filtered seawater). Test 3 consisted of metal treatments in an increasing series (no replicates) alongside 3 replicate controls. Seawater was spiked with metal solutions to achieve required concentration. Concentrations tested are recorded in excel datasheets as dissolved metal concentrations measured on day 0, and day 24. The average of the dissolved metal concentrations were used for further statistical analysis.
The age of C.armigera at test commencement was 25-30 days. Algal cells were centrifuged and washed to remove nutrient rich media, and test flasks were inoculated with between 1-3 x10^3 cells/mL. Cell densities in all toxicity tests were determined by flow cytometry. Toxicity tests with C. armigera were carried out over 23-24 days, with cell densities determined twice a week. The growth rate (cell division; u) was calculated as the slope of the regression line from a plot of log10 (cell density) versus time (h). Growth rates for all treatments were expressed as a percentage of the control growth rates.
The flow cytometer was also used to simultaneously measure changes in the following cellular parameters: chlorophyll a autofluorescence intensity (FL3), cell size (FSC) and cell complexity (SSC). The molecular stain BODIPY 493/503, was used to measure neutral lipid concentrations. Changes in cellular parameters were measured by applying a gate that captured greater than 95% of control cells in a region, R2. Changes in cellular parameters were observed in metal treatments as a shift of the cell population from the R2 region to R1 (for relative decreases) or to R3 (for relative increases). The proportion of cells in each region is expressed as a percentage of the total cell population.
The pH was measured on the first and last day of the test. Sub-samples (5 mL) for analysis of dissolved metal concentrations were taken from each treatment on 24. Sub-samples were filtered through an acid washed (10% HNO3, Merck) 0.45-microns membrane filter and syringe, and acidified to 0.2% with Tracepur nitric acid (Merck). Metal concentrations were determined using inductively coupled plasma-atomic emission spectrometry (ICP-AES; Varian 730-ES) for Cu, Cd, Pb, Ni and Zn. Detection limits for Cu, Cd, Pb, Ni and Zn were 1.0, 0.3, 3.2, 1.4, and 1.0 micrograms per litre, respectively.
Calculations of effect concentrations (EC 10 and 50) were made using the 'Dose Response Curve' package of R statistical analysis software. Concentration-response curves had several models applied to them, and were tested for best fit by comparing residual standard errors and Akaike's 'An Information Criterion' function . Generally, log-logistic models with 3 parameters provided the best fit.
Data for each toxicity test is combined in a single excel spreadsheet, "Cryothecomonas armigera single metal toxicity". The first worksheet is titled "Test Conditions" which provides information on the toxicity test, e.g. species and metals tested, dates, test conditions, as well as explanation of abbreviations, definitions of toxicity values etc. The second worksheet includes the raw cell densities determined in each flask, the calculated growth rates, and measured metal concentrations. The third worksheet contains the measured physiological parameters: Neutral lipid concentrations (BODIPY 493/503), chlorophyll a autofluorescence (FL3), cell complexity (SSC), and cell size (FSC). The final worksheet contains the output of statistical analysis; dose-response curves for each metal with applied log-logistic model and 95% confidence interval, a table summarising the effect concentrations (EC10 and EC50), and No Effect Concentration (NEC) is also provided.
The file "C. armigera combined.csv" contains rows representing individual exposures with columns for the metal treatment (Metal), averaged dissolved metal concentration for each exposure (Conc), growth rate (Growth), and growth rate as a percent of the control (Pcon). This data was used for data analysis in R statistics. Note that this contains data from all bioassays conducted with C. armigera, including those conducted by Francesca Gissi (doi:10.4225/15/551B2B65A73F3)
The script used for data analysis is provided in the document "R statistics script for C. armigera single metal.docx"
Simple
Identification info
- Alternate title
- Single metal toxicity to the Antarctic marine microalga Cryothecomonas armigera
- Date (Publication)
- 2017-06-21
- Edition
- 1
- Citation identifier
-
Dataset DOI
- Title
- Information and documentation - Digital object identifier system
- Date (Publication)
- 2012-04-23
- Citation identifier
- ISO 26324:2012
- Citation identifier
- doi:10.4225/15/5746938EC8C8B
Originator
Publisher
Principal investigator
Principal investigator
Principal investigator
Principal investigator
Collaborator
Collaborator
Collaborator
Collaborator
- Name
- CAASM Metadata
- Status
- Completed
Custodian
- Topic category
-
- Biota
- Environment
- Oceans
Extent
))
Extent
- Description
- Temporal Coverage
Temporal extent
- TimePeriod
- 2015-05-01 2016-02-29
- Title
- Chronic toxicity of five metals to the polar marine microalga Cryothecomonas armigera - Application of a new bioassay
- Date (Publication)
- 2017
- Citation identifier
- 228
- Citation identifier
- https://doi.org/10.1016/j.envpol.2017.05.034.
Author
- Name
- Environmental Pollution
- Page
- 211-221
- NASA/GCMD Earth Science Keywords
-
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE
- EARTH SCIENCE > OCEANS > WATER QUALITY > OCEAN CONTAMINANTS
- EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOTOXICOLOGY > TOXICITY LEVELS
- EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOTOXICOLOGY
- EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > TRACE ELEMENTS
- Keywords
-
- TOXICITY
- NASA/GCMD Earth Science Keywords
-
- FLOW CYTOMETRY
- NASA/GCMD Earth Science Keywords
-
- LABORATORY
- NASA/GCMD Earth Science Keywords
-
- AMD/AU
- AMD
- CEOS
- NASA/GCMD Earth Science Keywords
-
- GEOGRAPHIC REGION > POLAR
- OCEAN > SOUTHERN OCEAN
Resource constraints
- Use limitation
- This metadata record is publicly available.
Resource constraints
- Access constraints
- licence
- Other constraints
- These data are publicly available for download from the provided URL.
Resource constraints
- File type
- Portable Network Graphic
- Title
- Attribution 4.0 International (CC BY 4.0)
- Website
-
https://creativecommons.org/licenses/by/4.0/legalcode
Legal code for Creative Commons by Attribution 4.0 International license
- Use constraints
- licence
- Other constraints
- This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4326_microalga_toxicity when using these data.
- Language
- English
- Character encoding
- UTF8
Distribution Information
Distributor
Distributor
Distributor
- Fees
- Free
- Planned available datetime
- 2017-06-21T00:00:00
- Units of distribution
- kb
- Transfer size
- 193
- Distribution format
-
- Excel, csv, word
- OnLine resource
-
GET DATA
Download point for the data
- OnLine resource
-
PROJECT HOME PAGE
Public information for AAS project AAS_4326
- OnLine resource
-
VIEW RELATED INFORMATION
Citation reference for this metadata record and dataset.
Resource lineage
- Statement
- Data were collected in an Australian laboratory (CSIRO Land and Water, Centre for Environmental Contaminants Research, Lucas Heights, 2234, NSW) during May 2015 - February 2016. The tests used microalgal strains that had been previously collected from the Southern Ocean and are cultured within the microalgal collection at the Australian Antarctic Division (AAD). Daughter daughter cultures were transferred to CSIRO, where they were cultured for this work. Samples were analysed at the CSIRO Land and Water Laboratories, Lucas Heights, Sydney, NSW, Australia - using microalgal cultures sourced from the Southern Ocean, exact location not known.
- Hierarchy level
- Dataset
- Maintenance and update frequency
- As needed
- Maintenance note
- 2016-04-01 - record created by Dave Connell from a template completed by Darren Koppel. 2017-06-21 - record updated by Dave Connell to publicly release the data.
Metadata
- Metadata identifier
- string/AAS_4326_microalga_toxicity
- Language
- English
- Character encoding
- UTF8
Author
Sponsor
Owner
Type of resource
- Resource scope
- Dataset
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Citation identifier
- 42eb46ae-a929-4c8f-87ab-e3405f2d1bb3
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Date (Last Revision)
- 2016-04-01T01:14:42
Identifier
- Description
- metadata.extraction_date
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Citation identifier
- 8.6
- Metadata linkage
-
http://data.aad.gov.au/metadata/records/AAS_4326_microalga_toxicity
Point of truth for the metadata record
- Date info (Creation)
- 2016-04-01T00:00:00
- Date info (Last Update)
- 2017-06-21
Metadata standard
- Title
- ISO 19115-3
- Edition
- 2014
- Other citation details
- Version 1
- Title
- DIF to ISO 19115-1 Profile
Overviews
Spatial extent
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