K-Axis mesopelagic fish DNA-based diet analysis

High-throughput DNA-sequencing data for mesopelagic fish stomach contents sampled during the Kerguelen Axis voyage (January-Februay 2016).


Mesopelagic fish form an important link between zooplankton and higher trophic levels in Southern Ocean food webs, however their diets are poorly known. Most of the dietary information available comes from morphological analysis of stomach contents and to a lesser extent fatty acid and stable isotopes. DNA sequencing could substantially improve our knowledge of mesopelagic fish diets, but has not previously been applied. We used high-throughput DNA sequencing (HTS) of the 18S ribosomal DNA and mitochondrial cytochrome oxidase I (COI) to characterise stomach contents of four myctophid and one bathylagid species collected at the southern extension of the Kerguelen Plateau (southern Kerguelen Axis), one of the most productive regions in the Indian sector of the Southern Ocean. Diets of the four myctophid species were dominated by amphipods, euphausiids and copepods, whereas radiolarians and siphonophores contributed a much greater proportion of HTS reads for Bathylagus sp. Analysis of mitochondrial COI showed that all species preyed on Thysanoessa macrura, but Euphausia superba was only detected in the stomach contents of myctophids. Size-based shifts in diet were apparent, with larger individuals of both bathylagid and myctophid species more likely to consume euphausiids, but we found little evidence for regional differences in diet composition for each species over the survey area. The presence of DNA from coelenterates and other gelatinous prey in the stomach contents of all five species suggests the importance of these taxa in the diet of Southern Ocean mesopelagics has been underestimated to date. Our study demonstrates the use of DNA-based diet assessment to determine the role of mesopelagic fish and their trophic position in the Southern Ocean and inform the development of ecosystem models.

For more detail, see Clarke LJ, Trebilco R, Walters A, Polanowski AM, Deagle BE (2018). DNA-based diet analysis of mesopelagic fish from the southern Kerguelen Axis. Deep Sea Research Part II: Topical Studies in Oceanography. DOI: 10.1016/j.dsr2.2018.09.001.

Simple

Identification info

Alternate title
K-Axis mesopelagic fish DNA-based diet analysis
Date (Publication)
2019-04-09
Edition
1
Citation identifier
Dataset DOI

Title
Information and documentation - Digital object identifier system
Date (Publication)
2012-04-23
Citation identifier
ISO 26324:2012

Citation identifier
doi:10.26179/5cac19b608754

Originator

Deagle, B.E., Trebilco, R.

Publisher

Australian Antarctic Data Centre

Principal investigator

DEAGLE, BRUCE E.
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Principal investigator

TREBILCO, ROWAN
ACE CRC
Private Bag 80
Hobart
Tasmania
7050
Australia

Collaborator

DEAGLE, BRUCE E.
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Name
CAASM Metadata
Website
https://data.aad.gov.au/metadata/records/AAS_4313_KAXIS_FISH_DIET

Purpose
Characterise Southern Ocean mesopelagic fish diets using DNA data. In addition, test for differences in diet between fish species and regions (north and south of Kerguelen Axis voyage).
Status
Completed

Custodian

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Topic category
  • Biota
  • Oceans

Extent

N
S
E
W


Extent

Description
Temporal Coverage

Temporal extent

TimePeriod
2016-02-03 2016-03-07

Extent

Description
Vertical Extent - Depth

Vertical element

Minimum value
0
Maximum value
1000
Identifier
urn:x-ogc:def:cs:EPSG:6498
Name
Vertical CS. Axis: depth (D). Orientation: down. UoM: m.
Identifier
urn:x-ogc:def:axis:EPSG:113
Name
depth
Title
DNA-based diet analysis of mesopelagic fish from the southern Kerguelen Axis.
Date (Publication)
2018
Citation identifier
DOI:10.1016/j.dsr2.2018.09.001

Author

Clarke LJ, Trebilco R, Walters A, Polanowski AM, Deagle BE
Name
Deep Sea Research Part II: Topical Studies in Oceanography.
NASA/GCMD Earth Science Keywords
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > AMOEBOIDS > RADIOLARIANS
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHAUSIIDS (KRILL)
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > COPEPODS
Keywords
  • DNA
  • 18S RIBOSOMAL DNA
  • COI (CYTOCHROME OXIDASE I)
  • ILLUMINA MISEQ
  • MIDOC
NASA/GCMD Earth Science Keywords
  • ADS > Automated DNA Sequencer
NASA/GCMD Earth Science Keywords
  • R/V AA > R/V Aurora Australis
NASA/GCMD Earth Science Keywords
  • AMD
  • AMD/AU
  • CEOS
NASA/GCMD Earth Science Keywords
  • GEOGRAPHIC REGION > POLAR
  • OCEAN > SOUTHERN OCEAN > SOUTHERN KERGUELEN AXIS
  • OCEAN > SOUTHERN OCEAN > PRINCESS ELIZABETH TROUGH

Resource constraints

Use limitation
This metadata record is publicly available.

Resource constraints

File type
Portable Network Graphic
Linkage
Creative Commons by Attribution logo

Title
Attribution 4.0 International (CC BY 4.0)
Website
https://creativecommons.org/licenses/by/4.0/legalcode

Legal code for Creative Commons by Attribution 4.0 International license

Use constraints
licence
Other constraints
This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4313_KAXIS_FISH_DIET when using these data.
Language
English
Character encoding
UTF8

Distribution Information

Distributor

Distributor

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Distributor

Fees
free
Planned available datetime
2019-04-09T00:00:00
Units of distribution
kb
Transfer size
93
Distribution format
  • text, fasta, csv

OnLine resource
GET DATA

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OnLine resource
PROJECT HOME PAGE

Public information for AAS project 4313, 4344

OnLine resource
VIEW RELATED INFORMATION

Citation reference for this metadata record and dataset.

Resource lineage

Statement
Many COI samples have too few sequencing reads (counts) to reliably estimate diet based on this data. See detail in Clarke et al. (2018), Deep Sea Research II, https://doi.org/10.1016/j.dsr2.2018.09.001.
Hierarchy level
Dataset
Maintenance and update frequency
As needed
Maintenance note
2019-04-09 record created by Laurence Clarke

Metadata

Metadata identifier
string/AAS_4313_KAXIS_FISH_DIET

Language
English
Character encoding
UTF8

Author

CLARKE, LAURENCE
Australian Antarctic Division
203 Channel Highway
Kingston
TAS
7050
Australia

Sponsor

Australian Antarctic Division

Owner

AADC

Type of resource

Resource scope
Dataset

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
900f9d69-6453-4f05-83ad-cd911585fc7b

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Date (Last Revision)
2019-04-09T13:45:29

Identifier

Description
metadata.extraction_date

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
8.6

Metadata linkage
http://data.aad.gov.au/metadata/records/AAS_4313_KAXIS_FISH_DIET

Point of truth for the metadata record

Date info (Creation)
2019-04-09T00:00:00
Date info (Last Update)
2019-04-09

Metadata standard

Title
ISO 19115-3
Edition
2014
Other citation details
Version 1
Title
DIF to ISO 19115-1 Profile
 
 

Overviews

Spatial extent

N
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