Toxicity of single metals and metal mixtures to Antarctic Marine Copepods

This metadata record contains the results of two bioassays testing the response of Antarctic marine copepods to both individual and combined metals via 14 day toxicity tests. The tests were conducted during the 2012-2013 season at Davis Station, East Antarctica.

Three metals (cadmium, copper and zinc) were tested singularly and in metal mixture combinations. The concentrations used in the two tests are outlined in the excel spreadsheet (AAS4100_12-13_MixedMetalTox.xlsx). Tests were carried out in 70 mL plastic vials (exposure vials) that contained 50 mL of test solutions. Test solutions were prepared by mixing stock solutions with filtered (0.45 microns) sea water and were stored in a constant temperature cabinet at 0 plus or minus 1 degree C for at least 2 hours prior to the start of tests in order to get to the required test temperature. Each treatment included four replicates and each test included eight controls. Within each replicate vial, 9-12 copepods were carefully added. No additional air, food or water was provided over the test period. At five days a water change was completed by removing the old test solution and replacing it with freshly prepared test solution at the same concentration. The tests were carried out in a constant temperature cabinet set at 0 plus or minus 1 degree C on a 16:8 light:dark photoperiod over 14 days. The number of surviving copepods were counted in each test container, at the same time each day, for 10 days and then a final count was completed on day 14. Mortality was determined by observing the copepods over 20 seconds and if there was no movement they were considered dead.

Test solutions were sampled four times during the tests for measurement of metal concentrations. Samples were collected at day 0, day 5 pre-water change, day 5 post-water change and at day 10. Concentrations of the three test metals were determined in theses samples using Inductively-Coupled Plasma Optical Emission Spectrometer (ICP-OES) with appropriate matrix matched standards and blanks to ensure quality control.

For all analyses, measured metal concentrations (as opposed to nominal concentrations) were used. Point estimates, including LC10 and LC50 values, were determined using the maximum likelihood-probit method using the software ToxCalc (version 5.0.26 Tidepool Scientific Software). Point estimates were calculated at 4, 7, 10 and 14 days of exposure. Whenever the assumptions for the maximum likelihood-probit method were not met then the Trimmed Spearman-Karber Analysis was used.

Data are provided in an Excel workbook (filename: AAS4100_12-13_MixedMetalTox.xlsx). The first worksheet ("/Test Conditions") provides descriptive details for the tests and a key to abbreviations and units. Each worksheet includes a "This worksheet provides..." statement to assist interpretation of the data. A second data file is provided (filename: AAS4100_12-13_ToxCalc.xlsx) containing relevant test data from AAS4100_12-13_MixedMetalTox.xlsx, for input to ToxCalc software for analysis. This file also contains subsequent ToxCalc outputs, with key data (LC10 and LC50 values) provided in a summary worksheet.

Other support files provided are seven images of the test species (images by Frances Alexander) and two figures showing copepod response to test solutions (% survival) over the exposure period of the two tests.

Copepod samples were collected from the nearshore environment of Prydz Bay, offshore from Davis Station, on two days: 20 December 2012 and 9 January 2013. The 20 December collection was composed of Tisbe sp., collected from benthic habitats and the 9 January collection was composed of Paralabidocera Antarctica, collected from surface waters.

Two 14-day laboratory-based toxicity tests were conducted in the Davis laboratories. The test dates were: 2 - 16 January 2013 (test 01; using Tisbe sp., collected 20 December 2012) and 10 - 24 January 2013 (test 02; using P. Antarctica, collected 9 January 2013).

Identification info

Alternate title: Toxicity of single metals and metal mixtures to Antarctic Marine Copepods

Date (Publication): 2018-02-28

Edition: 1

Citation identifier

Dataset DOI

Title: Information and documentation - Digital object identifier system

Date (Publication): 2012-04-23

Citation identifier: ISO 26324:2012

Citation identifier: doi:10.4225/15/5518845AD05F7

Originator

Barnes, C., King, C.K. and Virtue, P.

Publisher

Australian Antarctic Data Centre

Principal investigator

BARNES, CASSANDRA

Institute of Marine and Antarctic Studies

Hobart

Tasmania

7001

Australia

0417386010

Principal investigator

KING, CATHERINE K.

Australian Antarctic Division

203 Channel Highway

Kingston

Tasmania

7050

Australia

+61 3 6232 3515

Principal investigator

VIRTUE, PATTI

Institute for Marine and Antarctic Studies

Private Bag 129

Hobart

Tasmania

7001

Australia

+61 3 6226 7470

Collaborator

BARNES, CASSANDRA

Institute of Marine and Antarctic Studies

Hobart

Tasmania

7001

Australia

0417386010

Collaborator

KING, CATHERINE K.

Australian Antarctic Division

203 Channel Highway

Kingston

Tasmania

7050

Australia

+61 3 6232 3515

Collaborator

VIRTUE, PATTI

Institute for Marine and Antarctic Studies

Private Bag 129

Hobart

Tasmania

7001

Australia

+61 3 6226 7470

Name: CAASM Metadata

Website: https://data.aad.gov.au/metadata/records/AAS_4100_Toxicity_Copepods

Status: Completed

Custodian

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)

Australian Antarctic Division

203 Channel Highway

Kingston

Tasmania

7050

Australia

+61 3 6232 3244

+61 3 6232 3351 (facsimile)

Topic category

Biota
Environment
Oceans

Extent

Description: Temporal Coverage

Temporal extent

TimePeriod: 2012-12-20 2012-12-20

Extent

Description: Temporal Coverage

Temporal extent

TimePeriod: 2013-01-02 2013-01-16

Extent

Description: Temporal Coverage

Temporal extent

TimePeriod: 2013-01-09 2013-01-09

Extent

Description: Temporal Coverage

Temporal extent

TimePeriod: 2013-01-10 2013-01-24

Title: Mixed Metal Combinations Increase Toxicity in Antarctic Marine Benthic Copepods.

Date (Publication): 2013

Author

Barnes, C.