TemperateReefBase Geonetwork Catalogue
Toxicity of copper, cadmium and zinc to Macquarie Island marine invertebrates
We investigated the toxicity of copper, zinc and cadmium to the following taxa: copepods Tigriopus angulatus (Lang) and Harpacticus sp. (Order Harpacticoida, Family Harpacticidae); flatworm Obrimoposthia ohlini (Bergendal) (Order Seriata, Family Procerodidae); bivalve Gaimardia trapesina (Lamarck) (Order Veneroida, Family Gaimardiidae); sea cucumber Pseudopsolus macquariensis (Dendy) (Order Dendrochirotida, Family Cucumriidae); sea star Anasterias directa (Koeler) (Order Forcipulatida, Family Asteriidae).
Sites chosen for the collection of invertebrates for this study were free of obvious signs of metal contamination, as verified by the analysis of seawater samples from collection sites by inductively coupled plasma optical emission spectrometry (ICP-OES).
Six invertebrate species were selected for toxicity tests to represent a range of taxa and ecological niches. Individuals of the copepod Tigriopus angulatus were collected using fine mesh dip nets from rock pools high on the intertidal zone. Individuals of the flatworm Obrimoposthia ohlini were collected from the undersides of boulders, high in the intertidal zone. The copepod Harpacticus sp. and bivalve Gaimardia trapesina were collected from several macroalgae species at high energy locations in the intertidal zone. Individuals of the sea cucumber Pseudopsolus macquariensis were collected from rocks from high energy locations from the intertidal to subtidal zones. Juveniles of the sea star Anasterias directa were collected from rocks in deep pools, low in the intertidal zone.
All experimental tests using O. ohlini, T. angulatus, P. macquariensis and A. directa were conducted at the AAD Kingston laboratories, while some tests with Harpacticus sp. and all tests with G. trapesina were conducted in the laboratory facilities on Macquarie Island. Adult life-stages were tested for all species except for P. macquairensis and A. directa in which juvenile stages were tested. Psedopsolus macquariensis released eggs in the aquarium which developed into juveniles prior to being used in tests, and juvenile A. directa were collected from the field.
Each test involved exposure to copper, zinc or cadmium solution under a static non-renewal test regime over 14 days. Five metal concentrations plus a control were used for each test, with 3-5 replicates of each concentration. Where possible, tests were replicated. Concentrations used in replicate tests sometimes varied, as species sensitivity information accrued in tests was used to optimise subsequent tests. Metal test solutions in seawater were prepared 24 hours prior to the addition of animals, using 500 micrograms/L CuSO4, 500 micrograms/L ZnCl2 and 500 micrograms/L Cd SO4 MilliQ stock solutions. Seawater was filtered to 0.45 microns and water quality parameters were measured using a TPS 90-FL multimeter at the start and end of tests. Dissolved oxygen (DO) was greater than 80% saturation, salinity 35 ppt plus or minus 0.5, and pH was ~8.1-8.3 at the start of tests. All experimental vials and glassware were acid washed with 10% nitric acid and rinsed with MilliQ three times before use. Metal concentrations were determined using ICP-OES; samples of test solutions were taken at the start (day 0) and end of tests (day 14), filtered through a 0.45 microns syringe filter and acidified with 1% ultra-pure nitric acid. Measured concentrations at the start of tests were within 96% of nominal concentrations. In order to estimate exposure concentrations, the measured concentrations at days 0 and 14 were averaged.
Tests were conducted in lidded plastic vials of varying sizes, depending on the size and number of individuals in the test. For both copepod species, there were 10 individuals per 50 mL in 70 mL vials; for P. macquariensis there were 8 individuals per 50 mL in 70 mL vials; and for O. ohlini, A. directa and G. trapesina, 10 individuals per 100 mL in 120 mL vials. Tests were conducted under a light-dark regime (at 2360 lux) of 18:6h light:dark in summer, 12:12 for tests for the rest of the year. Tests were kept in controlled temperature cabinets set at 6 degrees C, and temperatures within cabinets were monitored throughout the test using data loggers.
Vials were checked daily and survival recorded on days 1, 2, 4, 7, 10 and 14. Individuals were considered dead, and removed from test vials, when for G. trapesina adductor muscles no longer closed shell; O. ohlini were inactive and covered in mucous; P. macquariensis and A. directa tube feet were no longer moving; T. angulatus and Harpacticus sp. urosomes were perpendicular to prosomes.
Data are provided in a series of excel workbooks; one workbook per test species.
Simple
Identification info
- Alternate title
- Toxicity of copper, cadmium and zinc to Macquarie Island marine invertebrates
- Date (Publication)
- 2016-05-26
- Edition
- 2
- Citation identifier
-
Dataset DOI
- Title
- Information and documentation - Digital object identifier system
- Date (Publication)
- 2012-04-23
- Citation identifier
- ISO 26324:2012
- Citation identifier
- doi:10.4225/15/56369D520A118
Originator
Publisher
Principal investigator
Principal investigator
Principal investigator
Collaborator
Collaborator
- Name
- CAASM Metadata
- Status
- Completed
Custodian
- Topic category
-
- Biota
- Environment
- Oceans
Extent
))
Extent
- Description
- Temporal Coverage
Temporal extent
- TimePeriod
- 2012-04-01 2015-04-30
- NASA/GCMD Earth Science Keywords
-
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > COPEPODS
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > FLATWORMS/FLUKES/TAPEWORMS
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > MOLLUSKS > BIVALVES
- EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOTOXICOLOGY
- EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOTOXICOLOGY > BIOAVAILABILITY
- EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOTOXICOLOGY > SPECIES BIOACCUMULATION
- EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOTOXICOLOGY > TOXICITY LEVELS
- EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > TRACE ELEMENTS
- Keywords
-
- Toxicology
- Toxicity
- NASA/GCMD Earth Science Keywords
-
- ICP-ES > INDUCTIVELY COUPLED PLASMA EMISSION SPECTROSCOPY
- NASA/GCMD Earth Science Keywords
-
- FIELD INVESTIGATION
- FIELD SURVEYS
- NASA/GCMD Earth Science Keywords
-
- AMD/AU
- AMD
- CEOS
- NASA/GCMD Earth Science Keywords
-
- GEOGRAPHIC REGION > POLAR
Resource constraints
- Use limitation
- This metadata record is publicly available.
Resource constraints
- Access constraints
- licence
- Other constraints
- These data are publicly available for download from the provided URL.
Resource constraints
- File type
- Portable Network Graphic
- Title
- Attribution 4.0 International (CC BY 4.0)
- Website
-
https://creativecommons.org/licenses/by/4.0/legalcode
Legal code for Creative Commons by Attribution 4.0 International license
- Use constraints
- licence
- Other constraints
- This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4100_MI_marine_CuZnCd when using these data.
- Language
- English
- Character encoding
- UTF8
Distribution Information
Distributor
Distributor
Distributor
- Fees
- Free
- Planned available datetime
- 2016-05-26T00:00:00
- Units of distribution
- kb
- Transfer size
- 297
- Distribution format
-
- Excel
- OnLine resource
-
GET DATA
Download point for the data
- OnLine resource
-
PROJECT HOME PAGE
Public information for AAS project 4100
- OnLine resource
-
VIEW RELATED INFORMATION
Citation reference for this metadata record and dataset.
Resource lineage
- Statement
- No problems with the dataset. 2018-09-24 - The original datasheet was reformatted to fit IPT Biodiversity.AQ standard. The new datasheet "toxicityTestMarineOrganisms.csv" provides the datasetID, occurrenceID, country, countryCode, decimalLatitude, decimaLongitude, island, localitionID, eventDate, year, month, day, identificationQualifier, occurrenceStatus, and basisOfRecord. The lowest taxonomical rank of the species identified that could be determined is provided, after matched in WoRMS (World Register of Marine Species).
- Hierarchy level
- Dataset
- Maintenance and update frequency
- As needed
- Maintenance note
- 2015-10-30 - record created by Jessica Holan. 2016-05-26 - record updated by Dave Connell to release the data. 2018-09-24 - record updated by Dave Connell after a reformatted dataset was provided by Daniela Farias for GBIF/OBIS compliance.
Metadata
- Metadata identifier
- string/AAS_4100_MI_marine_CuZnCd
- Language
- English
- Character encoding
- UTF8
Author
Sponsor
Owner
Type of resource
- Resource scope
- Dataset
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Citation identifier
- ae70f0c2-9cc5-4f06-8d06-4b10058a9743
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Date (Last Revision)
- 2015-11-30T04:00:41
Identifier
- Description
- metadata.extraction_date
Alternative metadata reference
- Title
- gov.nasa.gsfc.gcmd
- Citation identifier
- 8.6
- Metadata linkage
-
http://data.aad.gov.au/metadata/records/AAS_4100_MI_marine_CuZnCd
Point of truth for the metadata record
- Date info (Creation)
- 2015-10-30T00:00:00
- Date info (Last Update)
- 2018-09-24
Metadata standard
- Title
- ISO 19115-3
- Edition
- 2014
- Other citation details
- Version 1
- Title
- DIF to ISO 19115-1 Profile
Overviews
Spatial extent
Provided by
