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CO2-induced changes in Antarctic phytoplankton communities using pigments

The data reports the pigment concentrations and results of CHEMTAX analysis for 2 summer seasons in Antarctic. In 2008/09 three experiments in which 6 x 650 l minicosms (polythene tanks) were used to incubate natural microbial communities (less than 200 um diameter) at a range of CO2 concentrations while maintained at constant light, temperature and mixing. The communities were pumped from ice-free water ~60 m offshore on 30/12/08, 20/01/09 and 09/02/09. These experiments received no acclimation to CO2 treatment. A further experiment was performed in 2014/15 using water helicoptered from ~ 1 km offshore amongst decomposing fast ice on 19/11/14. This experiment included a 5 day period during which the community was exposed top low light and the CO2 was gradually raised to the target value for each tank, followed by a two day period when the light was raised to an irradiance that was saturating but not inhibitory for photosynthesis.

A range of coincident measurements were performed to quantify the structure and function of the microbial community (see Davidson et al. 2016 Mar Ecol Prog Ser 552: 93–113, doi: 10.3354/meps11742 and Thomson et al 2016 Mar Ecol Prog Ser 554: 51–69, 2016, doi: 10.3354/meps11803).

The data provides a matrix of samples against component pigment concentration and the output from CHEMTAX that best explained the phytoplankton composition of the community based on the ratios of the component pigments.

For the 2008/09 experiments, samples were obtained every 2 days for 10, 12 and 10 days in experiments 1, 2 and 3 respectively. In 2014/15 samples were obtained from each incubation tank on days 1,3, 5, and 8 during th acclimation period and every 2 days until day 18 thereafter. For each sample a measured volume was filtered through 13 mm Whatman GF/F filters for 20 mins. Filters were folded in half, blotted dry, and immediately frozen in liquid nitrogen for analysis in Australia. Pigments were extracted, analysed by HPLC, and quantified following the methods of Wright et al. (2010). Pigments (including Chl a) were extracted from filters with 300 micro l dimethylformamide plus 50 micro l methanol, containing 140 ng apo-8'-carotenal (Fluka) internal standard, followed by bead beating and centrifugation to separate the extract from particulate matter. Extracts (125 micro l) were diluted to 80% with water and analysed on a Waters HPLC using a Waters Symmetry C8 column and a Waters 996 photodiode array detector. Pigments were identified by comparing retention times and spectra to a mixed standard sample from known cultures (Jeffrey and Wright, 1997), run daily before samples. Peak integrations were performed using Waters Empower software, checked manually for corrections, and quantified using the internal standard method (Mantoura and Repeta, 1997).

Simple

Identification info

Alternate title
CO2-induced changes in Antarctic phytoplankton communities using pigments
Date (Publication)
2022-09-20
Edition
1
Citation identifier
Dataset DOI

Title
Information and documentation - Digital object identifier system
Date (Publication)
2012-04-23
Citation identifier
ISO 26324:2012

Citation identifier
doi:10.4225/15/59928292a447f

Originator

Mckinlay, J., Davidson, A.T., Westwood, K.

Publisher

Australian Antarctic Division

Principal investigator

MCKINLAY, JOHN
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
03 62323622

Principal investigator

DAVIDSON, ANDREW TIMOTHY
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Principal investigator

WESTWOOD, KAREN
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
03 262323156
Name
CAASM Metadata
Website
https://data.aad.gov.au/metadata/records/AAS_4026_Pigments_CHEMTAX

Purpose
The data set enables direct comparison of the sensitivity of Coastal Antarctic phytoplankton communities to a range of CO2 concentrations within and among seasons.
Status
Completed

Custodian

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
+61 3 6232 3244
+61 3 6232 3351 (facsimile)
Topic category
  • Biota
  • Elevation
  • Oceans

Extent

N
S
E
W


Extent

Description
Temporal Coverage

Temporal extent

TimePeriod
2008-07-01 2015-06-30
Title
Effects of ocean acidification on rates of primary and bacterial production by natural Antarctic microbial communities.
Date (Publication)
2017

Author

Westwood K, Davidson A, Thomson P, van den Enden R, Ihnken S, Hollier M, Maher L, Wright S, Berry K
Name
J Exp Mar Biol Ecol
Title
Ocean acidification of a coastal Antarctic marine microbial community reveals a critical threshold for CO2 tolerance in phytoplankton productivity.
Date (Publication)
2017

Author

Deppeler S, Petrou K, Westwood K, Pearce I, McKinlay , Kai Schulz, K Andrew Davidson A
Name
Biogeochemistry
Title
Enhanced CO2 concentrations change the structure and function of Antarctic marine microbial communities.
Date (Publication)
2017
Citation identifier
552

Citation identifier
DOI: 10.3354/meps11742

Author

Davidson AT, McKinlay J, Westwood K, Thomson PG, van den Enden R, de Salas M, Wright S, Johnson R, Berry K
Name
Mar Ecol Prog Ser
Page
93-113
Title
Increased CO2 changes community composition of pico- and nano-sized protists and prokaryotes at a coastal Antarctic site.
Date (Publication)
2016
Citation identifier
554

Author

Thomson PG, Davidson AT, Maher L
Name
Mar Ecol Prog Ser
Page
51-69
Title
Ocean acidification changes the structure of an Antarctic coastal protistan community.
Date (Publication)
2017

Author

Hancock A, Davidson AT, McKinlay J, McMinn A, Schulz K, van den Enden R
Name
Biogeochemistry
NASA/GCMD Earth Science Keywords
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PIGMENTS > CHLOROPHYLL
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > PLANKTON > PHYTOPLANKTON
  • EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
Keywords
  • Minicosm
  • CHEMTAX
NASA/GCMD Earth Science Keywords
  • HPLC > High-Performance Liquid Chromatograph
NASA/GCMD Earth Science Keywords
  • LABORATORY
NASA/GCMD Earth Science Keywords
  • AMD/AU
  • AMD
  • CEOS
NASA/GCMD Earth Science Keywords
  • GEOGRAPHIC REGION > POLAR
  • OCEAN > SOUTHERN OCEAN
  • CONTINENT > ANTARCTICA > Davis Station

Resource constraints

Use limitation
This metadata record is publicly available.

Resource constraints

Access constraints
licence
Other constraints
These data are publicly available for download from the provided URL.

Resource constraints

File type
Portable Network Graphic
Linkage
Creative Commons by Attribution logo

Title
Attribution 4.0 International (CC BY 4.0)
Website
https://creativecommons.org/licenses/by/4.0/legalcode

Legal code for Creative Commons by Attribution 4.0 International license

Use constraints
licence
Other constraints
This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4026_Pigments_CHEMTAX when using these data.
Language
English
Character encoding
UTF8

Distribution Information

Distributor

Distributor

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
+61 3 6232 3244
+61 3 6232 3351 (facsimile)

Distributor

Fees
Free
Planned available datetime
2022-09-20T00:00:00
Units of distribution
MB
Transfer size
4.7
Distribution format
  • Excel

OnLine resource
EXTENDED METADATA

See the CHEMTAX metadata record

OnLine resource
GET DATA > DIRECT DOWNLOAD

Download the data

OnLine resource
PROJECT HOME PAGE

Public information for AAS project 4026

OnLine resource
VIEW RELATED INFORMATION

Citation reference for this metadata record and dataset.

Resource lineage

Statement
The data is of the highest quality using methods and interpretation overseen by Dr S. Wright
Hierarchy level
Dataset
Maintenance and update frequency
As needed
Maintenance note
2017-06-14 - record created by Andrew Davidson. 2022-09-20 - record updated by Dave Connell to publicly release the data.

Metadata

Metadata identifier
string/AAS_4026_Pigments_CHEMTAX

Language
English
Character encoding
UTF8

Sponsor

Australian Antarctic Division

Owner

AADC

Type of resource

Resource scope
Dataset

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
9016a674-fa1c-44ea-be91-2abf8156bf49

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Date (Last Revision)
2022-09-20T15:51:44

Identifier

Description
metadata.extraction_date

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
8.6

Metadata linkage
http://data.aad.gov.au/metadata/records/AAS_4026_Pigments_CHEMTAX

Point of truth for the metadata record

Date info (Creation)
2017-06-14T00:00:00
Date info (Last Update)
2022-09-20

Metadata standard

Title
ISO 19115-3
Edition
2014
Other citation details
Version 1
Title
DIF to ISO 19115-1 Profile
 
 

Overviews

Spatial extent

N
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Keywords


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