RNA sequence data from krill CO2 exposure experiment

We use RNA sequencing to investigate which genetic/physiological pathways in Antarctic krill are affected by increased CO2 levels. We carried out larval CO2 exposure experiments in March 2012 at the AAD aquarium. Two developmental stages were used (Calyptopis I and Furcilia V) and three CO2 levels (control, 1000 and 2000 ppm).


These were short term experiments (2 days) - since initial longer experiments starting with fertilized eggs resulted in differences in developmental stages between treatments and control which could confound the data. RNA was extracted from larvae and high-throughput RNA sequencing (RNA-seq) was carried out on 6 samples (2 stages * 3 treatments). Sequencing was carried out on an Illumina sequencer (Genome Analyzer II). We collected ~ 60 million sequence reads per sample (Data in FASTA format each read gives 100 base pairs of sequence), so a total of ~360 million reads (36 billion bp of data).

Simple

Identification info

Alternate title
RNA sequence data from krill CO2 exposure experiment
Date (Publication)
2016-07-01
Edition
1
Citation identifier
Dataset DOI

Title
Information and documentation - Digital object identifier system
Date (Publication)
2012-04-23
Citation identifier
ISO 26324:2012

Citation identifier
doi:10.4225/15/5775D60F1CB0D

Originator

Deagle, B.

Publisher

Australian Antarctic Data Centre

Principal investigator

DEAGLE, BRUCE
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Collaborator

DEAGLE, BRUCE
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Name
CAASM Metadata
Website
https://data.aad.gov.au/metadata/records/AAS_4015_krill_RNA

Status
Completed

Custodian

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Topic category
  • Biota
  • Oceans
  • Environment

Extent

N
S
E
W


Extent

Description
Temporal Coverage

Temporal extent

TimePeriod
2012-03-01 2012-09-30
NASA/GCMD Earth Science Keywords
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHAUSIIDS (KRILL)
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE
Keywords
  • OCEAN ACIDIFICATION
  • RNA
  • GENETICS
NASA/GCMD Earth Science Keywords
  • ADS > Automated DNA Sequencer
NASA/GCMD Earth Science Keywords
  • LABORATORY
NASA/GCMD Earth Science Keywords
  • AMD/AU
  • AMD
  • CEOS
NASA/GCMD Earth Science Keywords
  • GEOGRAPHIC REGION > POLAR
  • CONTINENT > ANTARCTICA
  • OCEAN > SOUTHERN OCEAN
  • CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA

Resource constraints

Use limitation
This metadata record is publicly available.

Resource constraints

Access constraints
licence
Other constraints
These data are publicly available for download. However, owing to their size, they will be placed on an FTP server at a user's request.

Resource constraints

File type
Portable Network Graphic
Linkage
Creative Commons by Attribution logo

Title
Attribution 4.0 International (CC BY 4.0)
Website
https://creativecommons.org/licenses/by/4.0/legalcode

Legal code for Creative Commons by Attribution 4.0 International license

Use constraints
licence
Other constraints
This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4015_krill_RNA when using these data.
Language
English
Character encoding
UTF8

Distribution Information

Distributor

Distributor

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Distributor

Fees
Free
Planned available datetime
2016-07-01T00:00:00
Units of distribution
GB
Transfer size
25
Distribution format
  • Text

OnLine resource
GET DATA

Request point for the data

OnLine resource
PROJECT HOME PAGE

Public information for AAS project AAS_4015

OnLine resource
VIEW RELATED INFORMATION

Citation reference for this metadata record and dataset.

Maintenance and update frequency
As needed
Maintenance note
2016-07-01 - record created by Dave Connell from a template completed by Bruce Deagle.

Metadata

Metadata identifier
string/AAS_4015_krill_RNA

Language
English
Character encoding
UTF8

Author

DEAGLE, BRUCE
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Sponsor

Australian Antarctic Division

Owner

AADC

Type of resource

Resource scope
Dataset

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
837d5822-51b3-4e44-81d8-a641cf0ec261

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Date (Last Revision)
2016-07-01T00:16:26

Identifier

Description
metadata.extraction_date

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
8.6

Metadata linkage
http://data.aad.gov.au/metadata/records/AAS_4015_krill_RNA

Point of truth for the metadata record

Date info (Creation)
2016-07-01T00:00:00
Date info (Last Update)
2017-04-26

Metadata standard

Title
ISO 19115-3
Edition
2014
Other citation details
Version 1
Title
DIF to ISO 19115-1 Profile
 
 

Overviews

Spatial extent

N
S
E
W


Keywords


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