Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates

These spreadsheets provide the sequences counts for the DNA groups found in the scats of Shy Albatross at Albatross Island, Tasmania, Australia.


Samples were collected during three stages of the breeding season: Incubation (Late September 2014), Brood (December 2014) and Chick-rearing (March 2014 and 2015). Scat samples were collected from breeding birds, chicks and non-breeders. 'Breeders' were identified as individuals incubating an egg or attending a chick, whereas 'non-breeders' were usually pairs that had reoccupied the colony and were building new practice nests with no chick present. Non-breeders in the colony include immature birds that have not yet bred and mature birds of breeding age that did not breed in a particular season (e.g. no partner or insufficient body condition)


Details of the molecular methods and synthesis of this data can be found in: McInnes, J.C., Alderman, R., Deagle, B.E. Lea, M-A., Raymond, B. and Jarman, S.N. 2016. Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates. Methods in Ecology and Evolution.

Simple

Identification info

Alternate title
Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates
Date (Publication)
2016-09-30
Edition
1
Citation identifier
Dataset DOI

Title
Information and documentation - Digital object identifier system
Date (Publication)
2012-04-23
Citation identifier
ISO 26324:2012

Citation identifier
doi:10.4225/15/57EDACF067ADF

Originator

McInnes, J.C., Deagle, B.E., Alderman, R., Jarman, S.N., Lea, M-A. and Raymond, B.

Publisher

Australian Antarctic Data Centre

Principal investigator

MCINNES, JULIE C.
Australian Antarctic Division
203 Channel Highway
Institute for Marine and Antarctic Studies
University of Tasmania
Private Bag 129
Kingston
Tasmania
7050
Australia

Principal investigator

DEAGLE, BRUCE E.
Australian Antarctic Division
203
Kingston
Tasmania
7050
Australia

Principal investigator

ALDERMAN, RACHAEL
Wildlife and Marine Conservation Section
Department of Primary Industry and Water
GPO BOX 44
Hobart
Tasmania
7001
Australia

Principal investigator

JARMAN, SIMON N.
CIBIO-InBIO
Research Center in Biodiversity and Genetic Resources
Universidade do Porto
Vairao
4485-661
Portugal

Principal investigator

LEA, MARY-ANNE
Institute for Marine and Antarctic Studies
University of Tasmania
Private Bag 129
Hobart
Tasmania
7001
Australia

Principal investigator

RAYMOND, BEN
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Collaborator

MCINNES, JULIE C.
Australian Antarctic Division
203 Channel Highway
Institute for Marine and Antarctic Studies
University of Tasmania
Private Bag 129
Kingston
Tasmania
7050
Australia

Collaborator

DEAGLE, BRUCE E.
Australian Antarctic Division
203
Kingston
Tasmania
7050
Australia

Collaborator

JARMAN, SIMON N.
CIBIO-InBIO
Research Center in Biodiversity and Genetic Resources
Universidade do Porto
Vairao
4485-661
Portugal
Name
CAASM Metadata
Website
https://data.aad.gov.au/metadata/records/AAS_4014_McInnes_et_al_2016

Purpose
Samples were collected to determine the affect that sample age, collection substrate, breeding stage and incubation duration had on the DNA amplification success and the proportion of food DNA. Using these data, optimised scat collection protocols were developed.
Status
Completed

Custodian

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Topic category
  • Biota
  • Oceans

Extent

N
S
E
W


Extent

Description
Temporal Coverage

Temporal extent

TimePeriod
2014-03-28 2015-03-31
Title
Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates.
Date (Publication)
2016

Author

McInnes, J.C., Alderman, R., Deagle, B.E. Lea, M-A., Raymond, B. and Jarman, S.N.
Name
Methods in Ecology and Evolution
NASA/GCMD Earth Science Keywords
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > BIRDS > ALBATROSSES/PETRELS AND ALLIES
Keywords
  • DNA METABARCODING
  • MISEQ GENOME SEQUENCING
  • SHY ALBATROSS
  • DIET
  • THALASSARCHE CAUTA
  • ALBATROSS ISLAND
NASA/GCMD Earth Science Keywords
  • AMD/AU
  • AMD
  • CEOS
NASA/GCMD Earth Science Keywords
  • GEOGRAPHIC REGION > POLAR
  • OCEAN > SOUTHERN OCEAN
  • CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA > Tasmania

Resource constraints

Use limitation
This metadata record is publicly available.

Resource constraints

Access constraints
licence
Other constraints
These data are publicly available for download from the provided URL.

Resource constraints

File type
Portable Network Graphic
Linkage
Creative Commons by Attribution logo

Title
Attribution 4.0 International (CC BY 4.0)
Website
https://creativecommons.org/licenses/by/4.0/legalcode

Legal code for Creative Commons by Attribution 4.0 International license

Use constraints
licence
Other constraints
Please contact the chief investigator of this work if you wish to use this data set for future analysis. This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4014_AAS_4014_McInnes_et_al_2016 when using these data.
Language
English
Character encoding
UTF8

Distribution Information

Distributor

Distributor

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

Distributor

Fees
Free
Planned available datetime
2016-09-30T00:00:00
Units of distribution
kb
Transfer size
75
Distribution format
  • Excel

OnLine resource
GET DATA

Download point for the data

OnLine resource
PROJECT HOME PAGE

Public information for AAS project AAS_4014

OnLine resource
VIEW RELATED INFORMATION

Citation reference for this metadata record and dataset.

Resource lineage

Statement
See the referenced paper for more information.
Hierarchy level
Dataset
Maintenance and update frequency
As needed
Maintenance note
2016-09-28 - record created by Julie McInnes.

Metadata

Metadata identifier
string/AAS_4014_McInnes_et_al_2016

Language
English
Character encoding
UTF8

Sponsor

Australian Antarctic Division

Owner

AADC

Type of resource

Resource scope
Dataset

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
654d1f2d-2d12-47a1-a423-27dffbef2e43

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Date (Last Revision)
2021-11-17T12:22:29

Identifier

Description
metadata.extraction_date

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
8.6

Metadata linkage
http://data.aad.gov.au/metadata/records/AAS_4014_McInnes_et_al_2016

Point of truth for the metadata record

Date info (Creation)
2016-09-28T00:00:00
Date info (Last Update)
2017-04-26

Metadata standard

Title
ISO 19115-3
Edition
2014
Other citation details
Version 1
Title
DIF to ISO 19115-1 Profile
 
 

Overviews

Spatial extent

N
S
E
W


Keywords


Provided by

Share on social sites

Access to the record in catalogue

Associated resources

Not available