Advection shapes Southern Ocean microbial assemblages independent of distance and environment effects

See the referenced paper for additional details.


Sampling. Sampling was conducted on board the RSV Aurora Australis during cruise V3 from 20 January to 7 February 2012. This cruise occupied a latitudinal transect from waters north of Cape Poinsett, Antarctica (65_ S) to south of Cape Leeuwin, Australia (37_ S) within a longitudinal range of 113-115_ E. Sampling was performed as described in ref. 29, with sites and depths selected to provide coverage of all major SO water masses. At each surface station, E250-560 l of seawater was pumped from E1.5 to 2.5m depth. At some surface stations, an additional sample was taken from the Deep Chlorophyll Maximum (DCM), as determined by chlorophyll fluorescence measurements taken from a conductivity, temperature and depth probe (CTD) cast at each sampling station. Samples of mesopelagic and deeper waters (E120-240 l) were also collected at some stations using Niskin bottles attached to the CTD. Sampling depths were selected based on temperature, salinity and dissolved oxygen profiles to capture water from the targeted water masses. Profiles were generated on the CTD descent, and samples were collected on the ascent at the selected depths. Deep water masses were identified by the following criteria: CDW 1/4 oxygen minimum (Upper Circumpolar Deep) or salinity maximum (Lower Circumpolar Deep); AABW 1/4 deep potential temperature minimum; AAIW 1/4 salinity minimum 18. The major fronts of the SO, which coincide with strong horizontal gradients in temperature and salinity 19,30, separate regions with similar surface water properties. The AZ lies south of the Polar Front (which was at 51_ S during sampling), whereas the PFZ lies between the Polar Front and the Subantarctic Front. In total, 25 samples from the AZ, PFZ, SAMW, AAIW, CDW and AABW were collected for this study (Fig. 1, Supplementary Data 1). Seawater samples were prefiltered through a 20-mm plankton net, biomass captured on sequential 3.0-, 0.8- and 0.1-mm 293-mm polyethersulphone membrane filters and filters immediately stored at _80 _C31,32.


DNA extraction and sequencing. DNA was extracted with a modified version of the phenol-chloroform method 31. Tag pyrosequencing was performed by Research and Testing Laboratory (Lubbock, USA) on a GS FLXb platform (Roche, Branford, USA) using a modification of the standard 926F/1392R primers targeting the V6-V8 hypervariable regions of bacterial and archaeal 16S rRNA genes (926wF: 50-AAA-CTY-AAA-KGA-ATT-GRC-GG-30 , 1,392 R: 50-ACG-GGCGGT-GTG-TRC-30). Denoising, chimera removal and trimming of poor quality read ends were performed by the sequencing facility.

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Identification info

Alternate title
Advection shapes Southern Ocean microbial assemblages independent of distance and environment effects
Date (Publication)
2013-10-07
Edition
1

Originator

Wilkins, D., van Sebille, E., Rintoul, S., Lauro, F.M., Cavicchioli, R., Yau, S. and Williams, T.J.

Publisher

Australian Antarctic Data Centre

Principal investigator

WILKINS, DAVID
The University of New South Wales
Sydney
NSW
Australia

Principal investigator

VAN SEBILLE, ERIK
Climate Change Research Centre
ARC Centre of Excellence for Climate System Science
The University of New South Wales
Sydney
NSW
2052
Australia

Principal investigator

RINTOUL, STEVE
CSIRO Marine and Atmospheric Research
GPO BOX 1538
Hobart
Tasmania
7001
Australia

Principal investigator

LAURO, FEDERICO M.
School of Biotechnology and Biomolecular Sciences
University of New South Wales
Sydney
NSW
2052
Australia

Principal investigator

CAVICCHIOLI, RICK
School Of Microbiology and Immunology
University Of New South Wales
Sydney
NSW
2052
Australia

Principal investigator

YAU, SHEREE
School of Biotechnology and Biomolecular Sciences
Cavicchioli Research Lab
University of New South Wales
Sydney
NSW
Australia

Principal investigator

WILLIAMS, TIM
School of Biotechnology and Biomolecular Sciences
Cavicchioli Research Lab
University of New South Wales
Sydney
NSW
Australia

Collaborator

CAVICCHIOLI, RICK
School Of Microbiology and Immunology
University Of New South Wales
Sydney
NSW
2052
Australia
Name
CAASM Metadata
Website
https://data.aad.gov.au/metadata/records/AAS_3145_Advection

Status
Completed

Custodian

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia
Topic category
  • Biota
  • Oceans

Extent

N
S
E
W


Extent

Description
Temporal Coverage

Temporal extent

TimePeriod
2012-01-20 2012-02-07
Title
Advection shapes Southern Ocean microbial assemblages independent of distance and environment effects.
Date (Publication)
2013
Citation identifier
4

Citation identifier
doi: 10.1038/ncomms3457.

Author

Wilkins, D., van Sebille, E., Rintoul, S.R., Lauro, F.M., and Cavicchioli, R.
Name
Nature Communications
Issue identification
2457
NASA/GCMD Earth Science Keywords
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CHLOROPHYLL
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PIGMENTS > CHLOROPHYLL
Keywords
  • Microbes
  • Microbial assemblages
  • Advection
  • DNA
NASA/GCMD Earth Science Keywords
  • NISKIN BOTTLES
  • CTD > Conductivity, Temperature, Depth
NASA/GCMD Earth Science Keywords
  • R/V AA > R/V Aurora Australis
NASA/GCMD Earth Science Keywords
  • AMD
  • AMD/AU
  • CEOS
NASA/GCMD Earth Science Keywords
  • OCEAN > SOUTHERN OCEAN
  • CONTINENT > ANTARCTICA
  • GEOGRAPHIC REGION > POLAR

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This metadata record is publicly available.

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Attribution 4.0 International (CC BY 4.0)
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Legal code for Creative Commons by Attribution 4.0 International license

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This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_3145_Advection when using these data.
Language
English
Character encoding
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AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)
Australian Antarctic Division
203 Channel Highway
Kingston
Tasmania
7050
Australia

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Fees
Free
Planned available datetime
2013-10-07T00:00:00

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Fees
Free
Planned available datetime
2013-10-07T00:00:00
Units of distribution
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Transfer size
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Distribution format
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Units of distribution
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Transfer size
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Distribution format
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Maintenance and update frequency
As needed
Maintenance note
2013-10-07 - record created by Dave Connell from a template completed by Rick Cavicchioli.

Metadata

Metadata identifier
string/AAS_3145_Advection

Language
English
Character encoding
UTF8

Author

CAVICCHIOLI, RICK
School Of Microbiology and Immunology
University Of New South Wales
Sydney
NSW
2052
Australia

Sponsor

Australian Antarctic Division

Owner

AADC

Type of resource

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Dataset

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
98aa0138-b8e7-40df-ba54-1469e2cfe61c

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Date (Last Revision)
2015-11-30T03:58:56

Identifier

Description
metadata.extraction_date

Alternative metadata reference

Title
gov.nasa.gsfc.gcmd
Citation identifier
8.6

Metadata linkage
http://data.aad.gov.au/metadata/records/AAS_3145_Advection

Point of truth for the metadata record

Date info (Creation)
2013-10-06T00:00:00
Date info (Last Update)
2017-04-26

Metadata standard

Title
ISO 19115-3
Edition
2014
Other citation details
Version 1
Title
DIF to ISO 19115-1 Profile
 
 

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