Climate change and urchin fertilisation and the effect on the growth rate of juvenile Abatus sp.

The effect of pH, temperature and sperm concentration on the fertilisation of Sterechinus neumayeri was investigated. Adult Sterechinus neumayeri were collected from Ellis Fjord Narrows between December and January 2011-12 and held in the Ecotox Field Aquarium Module until used. Between 3-4 male and female individuals were spawned using 0.5M KCl and gametes were collected separately before being fertilised in treatment.

The data set shows the percentage of fertilised and non-fertilised eggs of Sterechinus neumayeri scored at 20h post-fertilisation. Eggs were fertilised in various combinations of pH, temperature and sperm concentration treatments (pH: 8.0 (Control), 7.8 and 7.6; Temperature: 1 degrees C (Control), 3 degrees C and 5 degrees C; Sperm concentration (sperm:egg ratio): 1000:1 (Control), 750:1, 250: 1, 50:1 and 5:1). At 20h post fertilisation, 5 ml aliquot was removed from fertilisation vials and eggs were counted and determined if they were fertilised or not. Seawater parameters of treatments were measured at the start and end of the experiment.

Detailed information of the spreadsheets are as follows:

Seawater Parameters column headings:

Temperature - measured in degrees C , shows the temperature treatments used

pH - shows the pH levels used

Subheading pH - pH level measured for the day using NIST certified buffers

Subheading MV - pH level measured for the day in millivolts

Subheading Total pH - total pH level in seawater obtained from MV measurements

Subheading Temp - temperature of seawater measured for the day

1 deg C column headings:

Experiment - number of experiments

pH - shows the pH for each treatment

Sperm Concentration - shows the sperm concentration used for each treatment in a egg:sperm ratio

Rep - shows the number of replicates per experiment

Unfertilised eggs - eggs without visible fertilisation envelope and no cleavage after 20h

Fertilised eggs - eggs with visible fertilisation envelope and/or cleavage after 20h

Fertilised deformed eggs - eggs with visible fertilisation envelope but deformed

Total eggs - total eggs scored (whether fertilised or unfertilised)

% Fertilised - fertilised eggs (deformed and non-deformed)/Total eggs

3 deg C and 5 deg C have the same column headings as 1 deg C.

AAS3134 Abatus sp Growth Experiment Davis 2011-12:

The effect of pH and temperature on the growth rate of juvenile Abatus ingens and Abatus shackletoni were investigated. Adult Abatus were collected off Airport Beach in waters 4-5m depth.

Data set shows the growth rate of juveniles of Abatus ingens and Abatus shackletoni after a 4-week exposure to various combinations of pH and temperature. Juveniles of each species was removed from maternal pouches and photographed on the oral side before being exposed to combinations of pH (8.0 (Control), 7.8 and 7.6) and temperature (-1 degrees C (Control) and 1 degrees C) levels. They were incubated in treatments for 4 weeks before being removed and rephotographed. The lengths of 10 spines per juvenile were measured in the pre- and post-experiment photographs using ImageJ and the difference calculated to get a growth rate per juvenile. Seawater parameters of treatments were measured at the beginning of the experiment and subsequently once a day until the end of the experiment.

Detailed information of the spreadsheets are as follows:

A ingens (pre-exp) i.e. juvenile Abatus ingens spine lengths measured before exposure to experimental treatments. Column headings are:

Spine number and length (mm): Length of each spine (1 - 10) measured per juvenile in mm.

R1 - R12: Number of juveniles

A ingens (post-exp) i.e. juvenile Abatus ingens spine lengths measured after 4-week exposure to experimental treatments. Column headings are identical to the above.

A shackletoni (pre-exp) i.e. juvenile Abatus shackletoni spine lengths measured before exposure to experimental treatments. Column headings are identical to the above.

A shackletoni (post-exp) i.e. juvenile Abatus shackletoni spine lengths measured after 4-week exposure to experimental treatments. Column headings are identical to the above.

2011-12 Aquarium pH and temp main headings show different treatment parameters. Column sub-headings are:

Date - Date of measured seawater parameters

Salinity - salinity of seawater measured

Ppm - Amount of CO2 gas pumped into water recorded in parts per million

pH - measured pH of seawater using NIST-certified buffers

MV - pH of seawater recorded in millivolts

Total pH - total pH of seawater derived from MV

Temp - Temperature of seawater measured in degrees C.

Identification info

Alternate title: Climate change and urchin fertilisation and the effect on the growth rate of juvenile Abatus sp.

Date (Publication): 2013-03-21

Edition: 2

Citation identifier

Dataset DOI

Title: Information and documentation - Digital object identifier system

Date (Publication): 2012-04-23

Citation identifier: ISO 26324:2012

Citation identifier: doi:10.4225/15/514A7E3CA9CEB

Originator

Ho, M.

Publisher

Australian Antarctic Data Centre

Principal investigator

HO, MELANIE

University of Sydney

Department of Anatomy and Histology F13

Sydney

NSW

2006

Australia

Collaborator

HO, MELANIE

University of Sydney

Department of Anatomy and Histology F13

Sydney

NSW

2006

Australia

Name: CAASM Metadata

Website: https://data.aad.gov.au/metadata/records/AAS_3134_urchins_climate_change

Status: Completed

Custodian

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)

Australian Antarctic Division

203 Channel Highway

Kingston

Tasmania

7050

Australia

+61 3 6232 3244

+61 3 6232 3351 (facsimile)

Topic category

Biota
Environment
Oceans

Extent

Description: Temporal Coverage

Temporal extent

TimePeriod: 2011-12-01 2012-02-28