Project 3054 Season 1 Hydrocarbon ecotoxicological experiments Paramoera walkeri

Ecotoxicological tests were done at Davis and Casey Stations in 2009/10, 2010/11 and 2011/12 summer seasons under AAS Project 3054 to test the sensitivity of near-shore marine invertebrates to fuels in seawater. The three fuel types used in this project were: Special Antarctic Blend diesel (SAB), Marine Gas Oil diesel (MGO) and an intermediate grade (180) of marine bunker fuel oil (IFO).

This dataset contains the results of tests with the near-shore amphipod species Paramoera walkeri exposed to WAFs of SAB, MGO and IFO 180 (specified below) conducted at Davis Station in 2009/10 summer (Season 1).

Test treatments were obtained by experimentally mixing fuel and seawater in temperature control cabinets at -1&#176;C to prepare a mixture of fuel hydrocarbons in filtered seawater (FSW) termed the water accommodated fraction (WAF). WAF was produced by adding fuel to seawater in 5 L or 10 L Pyrex glass bottles using a ratio of 1:40 fuel : FSW. This mixture was stirred at slow speed with minimal vortex on a magnetic stirrer. The water portion was then drawn from beneath the fuel. Test treatments consisted of undiluted 100% WAF and dilutions of 10% and 1% of WAFs in FSW.

Toxicity tests were conducted in open glass vessels in temperature controlled cabinets. Mortality and/or sub-lethal effects were observed at endpoints of 24 h, 48 h, 96 h, 7 d, 14 d, and 21 d. Treatments were renewed at 7 d intervals. Water quality data was collected at each water change.

Hydrocarbon concentrations in WAFs were determined from replicate experiments to measure THC in WAFs over time (Dataset AAS_3054_THC_WAF).

WAF exposure concentrations for each test endpoint were derived from these hydrocarbon tests to account for depletion of hydrocarbons from test treatments and any renewal of treatments. An integrated concentration was calculated from measured hydrocarbon concentrations weighted to time. These integrated THC concentrations for endpoints from 24h to 21d are contained in dataset AAS_3054_THC_WAF_integrated_conc_09_10 and are the exposure concentrations used for analysis of sensitivity.

Species tested;

Paramoera walkeri amphipod; adults

This dataset consists of Excel spreadsheets. The file name code for invertebrate tests is;

Project number_Season_Taxa_Test name

Eg AAS_3054_09_10_amphipod_1PWA1

Project number : AAS_3054

Season : 2009/10 season

Taxa: amphipod

Test name: 1 for Season 1, PW for genus and species, A for adult, 1 for Test 1

Spreadsheets contain the results of tests with this species. Where replicate tests were conducted, each experiment is on a separate spreadsheet. The worksheet labelled 'Test conditions' shows details of Test name, dates, animal collection details, laboratory holding conditions, details of water accommodated fractions (WAF), test conditions, scoring criteria and water quality data. The worksheet labelled 'Counts' has columns for Replicate number and columns with the Score for all the animals in that replicate at every time endpoint. A full description of the scoring criteria is on the 'Test conditions' worksheet. Totals, means and standard deviations are calculated for each treatment. The worksheet labelled 'Totals, means, percent, StDev' has calculations of Survival, Unaffected, including mean and standard deviation, Percent Survival and Unaffected including means and standard deviation. Amphipod tests also show the Total number of moults in each treatment.

Samples were collected at the following locations:

- Airport Beach, Davis, Vestfold Hills

Identification info

Alternate title: Project 3054 Season 1 Hydrocarbon ecotoxicological experiments Paramoera walkeri

Date (Publication): 2018-04-05

Edition: 1

Citation identifier

Dataset DOI

Title: Information and documentation - Digital object identifier system

Date (Publication): 2012-04-23

Citation identifier: ISO 26324:2012

Citation identifier: doi:10.4225/15/54EFE0F5EA37A

Originator

Brown, K.E., King, C.K., Harrison, P.

Publisher

Australian Antarctic Data Centre

Principal investigator

BROWN, KATHRYN EUNICE

Marine Ecology Research Centre

Southern Cross University

PO Box 157

Lismore

NSW

2480

Australia

Principal investigator

KING, CATHERINE K.

Australian Antarctic Division

203 Channel Highway

Kingston

Tasmania

7050

Australia

+61 3 6232 3515

Principal investigator

HARRISON, PETER

Southern Cross University

Lismore

New South Wales

2480

Australia

+61 2 6620 3774

Collaborator

BROWN, KATHRYN EUNICE

Marine Ecology Research Centre

Southern Cross University

PO Box 157

Lismore

NSW

2480

Australia

Name: CAASM Metadata

Website: https://data.aad.gov.au/metadata/records/AAS_3054_09_10_ecotox

Status: Completed

Custodian

AU/AADC > Australian Antarctic Data Centre, Australia - AADC, DATA OFFICER (DATA CENTER CONTACT)

Australian Antarctic Division

203 Channel Highway

Kingston

Tasmania

7050

Australia

+61 3 6232 3244

+61 3 6232 3351 (facsimile)

Topic category

Biota
Environment
Oceans

Extent

Description: Temporal Coverage

Temporal extent

TimePeriod: 2010-01-20 2010-06-02

Title: Assessing toxicity of diesel and fuel oil on early life stages of Antarctic marine invertebrates

Date (Publication): 2012

Author

Brown K., King C., George S., Lane A., Harrison P.

Name: Proceedings of the 2nd Society for Environmental Toxicology and Chemistry (SETAC) Australasia Conference

Other citation details: Brisbane, Australia, 4-6 July 2012.

Title: Assessing toxicity of diesel and fuel oil on early life stages of Antarctic marine invertebrates

Date (Publication): 2012

Author

Brown K., King C., George S., Lane A., Harrison P.

Name: XXXII Open Science Conference of the Scientific Committee on Antarctic Research

Other citation details: Portland, Oregon, USA, 15-19 July 2012

Title: Sensitivity of the Antarctic amphipod Paramoera walkeri to diesel and fuel oil: Lethal and behavioural impacts

Date (Publication): 2013