Phenotypic plasticity in habitat-forming seaweeds subject to latitudinal transplant

The phenotypic plasticity of habitat-forming seaweeds was investigated with a transplant experiment in which juvenile Ecklonia radiata and Phyllospora comosa were transplanted from NSW (warm conditions) to Tasmania (cool conditions) and monitored for four months. We used multiple performance indicators (growth, photosynthetic characteristics, pigment content, chemical composition, stable isotopes, nucleic acids) to assess the ecophysiology of seaweeds before and following transplantation between February 2012 and June 2012.

Identification info

Date (Creation): 2016-02-22

Citation identifier

doi:10.4226/77/572171cf5940f

Title: Information and documentation - Digital object identifier system

Date (Publication): 2016-04-28

Citation identifier: ISO 26324:2012

Citation identifier: https://doi.org/10.4226/77/572171cf5940f

Principal investigator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Flukes, Emma

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS)

Private Bag 129

Hobart

Tasmania

7001

Australia

ORCID ID >

Collaborator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Johnson, Craig, Prof.

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS)

Private Bag 129

Hobart

Tasmania

7001

Australia

+61 3 6226 2582

ORCID ID >

Collaborator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Wright, Jeffrey, Dr

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS)

Private Bag 1370

Launceston

Tasmania

7250

Australia

+61 3 6324 3848

ORCID ID >

Credit: Australian Research Council (ARC) grant DP1096573

Status: Completed

Principal investigator

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Flukes, Emma, Dr

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS)

Private Bag 129

Hobart

Tasmania

7001

Australia

ORCID ID >

Temporal resolution: P0Y1M0DT0H0M0S

Topic category

Biota

Extent

Temporal extent

Time period: 2012-02-25 2012-06-30

Vertical element

Minimum value: 5

Maximum value: 10

Identifier: EPSG:5715

Name: MSL depth

Maintenance and update frequency: Not planned

Resource format

Title: CSV

Date

Edition: n/a

Keywords (Theme)

phycology
kelp
PAM fluorometry
ecophysiology
RNA:DNA
transplantation
ecosystem engineer
phenotypic plasticity
in situ measurements

Keywords (Taxon)

Ecklonia radiata
Phyllospora comosa

NASA/GCMD Keywords, Version 8.5

Keywords (Discipline)

Temperate Reef

Australian and New Zealand Standard Research Classification (ANZSRC): Fields of Research

AODN Platform Vocabulary

diver

Keywords (Theme)

Relative electron transport rate
Alpha
Minimum saturating irradiance
Maximum quantum yield (dark-acclimated sample) - also Fv/Fm
Tissue concentration of fucoxanthin
Tissue concentration of chlorophyll a
Tissue concentration of chlorophyll c
Ratio of tissue RNA to DNA concentration
Tissue concentration of RNA
Tissue concentration of DNA
Tissue percentage carbon
Tissue percentage nitrogen
Ratio of tissue carbon to nitrogen
δ13C
δ15N
Blade area

AODN Instrument Vocabulary

Resource constraints

Classification: Unclassified

Resource constraints

Linkage: http://i.creativecommons.org/l/by/4.0/88x31.png
License Graphic

Title: Creative Commons Attribution 4.0 International License

Website: http://creativecommons.org/licenses/by/4.0/
License Text

Other constraints: Cite data as: Flukes, E.B., Johnson, C.R. & Wright, J.T. (2016). Phenotypic plasticity in habitat-forming seaweeds subject to latitudinal transplant. Institute for Marine and Antarctic Studies, University of Tasmania. doi:10.4226/77/572171cf5940f

Other constraints: The data described in this record are the intellectual property of the University of Tasmania through the Institute for Marine and Antarctic Studies.

Language: English

Character encoding: UTF8

Content Information

Content type: Physical measurement

Description: measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name: Relative electron transport rate

Name: rETRmax

Name: no units (relative units)

Description: measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name: Alpha

Name: Alpha

Name: nil

Description: measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name: Minimum saturating irradiance

Name: Ek

Name: µmol photons m-2 s-1

Description: measured using blue diving-PAM fluorometer (contact researcher for PAM settings)

Name: Maximum quantum yield (dark-acclimated sample) - also Fv/Fm

Name: Fv.Fm

Name: nil (ratio)

Name: Tissue concentration of fucoxanthin

Name: Fucoxanthin

Name: mg g-1 wet weight

Name: Tissue concentration of chlorophyll a

Name: ChlA

Name: mg g-1 wet weight

Name: Tissue concentration of chlorophyll c

Name: ChlC

Name: mg g-1 wet weight

Description: Both RNA and DNA concentrations were extracted from a single sample of tissue

Name: Ratio of tissue RNA to DNA concentration

Name: RNA.DNA

Name: nil (ratio)

Description: measured using extraction method developed specifically for this data

Name: Tissue concentration of RNA

Name: RNA

Name: µg g wet weight-1

Description: measured using extraction method developed specifically for this data

Name: Tissue concentration of DNA

Name: DNA

Name: µg g wet weight-1

Name: Tissue percentage carbon

Name: per_C

Identifier: http://vocab.nerc.ac.uk/collection/P06/current/UPCT

Name: Percent

Name: Tissue percentage nitrogen

Name: per_N

Identifier: http://vocab.nerc.ac.uk/collection/P06/current/UPCT

Name: Percent

Description: measured using mass spectrometry

Name: Ratio of tissue carbon to nitrogen

Name: C.N

Name: nil (ratio)

Description: Pee Dee Belemnite as standard

Name: δ13C

Name: d13C

Name: ‰

Description: Air as standard

Name: δ15N

Name: d15N

Name: ‰

Description: measured as length x width of blades/lamina

Name: Blade area

Name: Blade_area

Name: mm^2

Distribution Information

Distribution format

CSV (.csv)

OnLine resource: DATA - Ecophysiological measurements before and after 4 mo. transplant (.csv) [direct download]

OnLine resource: DATA - Growth of seaweeds at monthly intervals (.csv) [direct download]

OnLine resource: DATA - Raw PAM fluorometry RLC outputs at monthly intervals (zipped .csv) [direct download]

Resource lineage

Statement: Juvenile Ecklonia radiata and Phyllospora comosa were transplanted from NSW-Tas and Tas-Tas. Seaweeds were first collected and kept overnight in the laboratory where they were attached to lengths of 6 mm diameter polypropylene rope by inserting holdfasts into the weave of the rope. Phyllospora were outplanted at a depth of ~5m and Ecklonia at ~10m in Fortescue Bay, Tasmania on 28th/29th February 2012. Measurements of growth and PAM fluorometry were made monthly, while other destructive ecophysiological measurements were made in situ at the time of collection, and 123 days following transplantation. The following parameters were measured: growth, PAM fluorometry photosynthetic characteristics, pigment content (chlorophyll a, c, fucoxanthin), tissue chemistry (δ13C, δ15N, % C, % N, C:N ratios), and nucleic acid characteristics (absolute RNA and DNA, RNA:DNA ratios). Terminal measurements were made on 30th June 2012. Pigments were measured using spectrophotometry and HPLC. Tissue chemistry was measured using mass spectrometry. Nucleic acids were measured using a novel technique described in Flukes et al. 2016. See Flukes et al. 2016 for a full description of all methodologies. NOTE: in data file "otherparamdata.csv", "start" sampling refers to in situ samples, "end" sampling refers to terminal measurements at t = 123 days. In data files "growthdata.csv" and contents of "raw_PAM_RLC_output", sample times refer to time since transplantation (days).

Hierarchy level: Dataset

Hierarchy level: Dataset

Platform

Identifier

Code: diver

Instrument

Identifier

Code: fluorometers

Hierarchy level: Dataset

Instrument

Identifier

Code: high performance liquid chromatographs

Hierarchy level: Dataset

Instrument

Identifier

Code: spectrophotometers

Hierarchy level: Dataset

Instrument

Identifier

Code: gas chromatograph mass spectrometers

Metadata

Metadata identifier: 6cbf0c73-1ade-4c1d-a83f-555118cf15ea

Language: English

Character encoding: UTF8

Author

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - Flukes, Emma

Point of contact

Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) - IMAS Data Manager

Type of resource

Resource scope: Dataset

Metadata linkage: https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/6cbf0c73-1ade-4c1d-a83f-555118cf15ea
Point of truth URL of this metadata record

Date info (Creation): 2020-08-14T12:02:23

Date info (Revision): 2020-08-14T12:02:23

Metadata standard

Title: ISO 19115-3:2018

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